Larval rearing of Macrobrachium rosenbergii (De Man) in artificial sea water

Larvae of Macrobrachium rosenbergii were successfully reared in artificial sea water prepared in fresh ground water. The water was circulated through a biological filter by means of air-lift pumps for a period of one week to remove the undissolved particles prior to use in the hatchery operation. The experiments were initiated during 1989 and the hatchery has been working on pilot scale since June, 1990. The larvae in all the experiments were fed with egg-custard, Mona and Artemia nauplii. The survival rate varied from 5 to 52% in the 12 experiments. These findings can add to the development of hatcheries in the inland areas which can further boost the popularization of giant freshwater prawn farming.

The myogenic automatism of the systemic heart of Octopus vulgaris (Cephalopoda: Coleoidea): evidence for localized stretch sensitivity and pacing activity

The localization of the stretch sensitivity and myogenic automatism in the systemic heart of Octopus vulgaris has been studied on an isolated preparation in which the ventricle was zoned by ligatures. Each region has been submitted to two different levels of internal hydrostatic pressure (1 and 2 kPa). Only the two atrio-ventricular regions were able to contract regularly when submitted to internal pressure, with a frequency dependent from the pressure value, while the ventricle-aortic region was insensitive to the stretching by internal pressure. This result supports the hypothesis that the automatism in this heart is localized. Electrocardiogram recordings from different areas of an isolated and perfused preparation of the systemic heart ventricle are also reported, which suggest that the electrical activity of the ventricle originates in two narrow areas near the atrio-ventricular valves.

Determination of ubiquinone and tocopherol in heart, liver and muscle tissues of fresh water fish, Rohu (Labeo rohita, Hamilton)

The liver, heart and muscle tissues of Rohu, (Labeo rohita, Hamilton) were examined for their ubiquinone (UQ) and tocopherol contents. These three tissues contained respectively 11.60, 3.94 and 0.19 mg of ubiquinone and 10.16, 5.32 and 3.58 mg of tocopherol per 100 g. The 4% (V/V) of diethyl ether (EE) in light petroleum ether (PE) fractions of all three tissues on paper chromatographic separation gave spots having the same Rf value as standard ubiquinone-50 (UQ 10). Both the 4% and 6% (V/V) diethyl ether in light petroleum ether fraction of liver, heart and muscle tissues gave a single spot with the same Rf value as α – tocopherol.

Effects of artificial feeds on production of fishes in polyculture

A study on the effects of artificial feeds on the growth and production of fishes in polyculture in 6 ponds along with some limnological conditions was conducted. Species of Indian and Chinese major carps (Labeo rohita, Catla catla, Cirrhinus mrigala, Hypophthalmicthys molitrix) and catfishes (Clarias batrachus, Clarias gariepinus) were stocked in 6 ponds. Stocking rate in both cases were 32044 fingerlings per hectare. Ratio of species of Rui:Catla:Mrigal:Silver carp:African Magur:Local Magur=25%:25%:5%:25%:14%:6%. Fertilization and artificial feeds were given in 3 ponds (treatment I) and only fertilization was done in other 3 ponds (treatment II). Average yield/ha/yr was 7.903 m.ton in case of fertilization and artificial feeding application and 3.374 m.ton in case of only fertilization application. Urea, TSP and cow dung were applied fortnightly at the rates of 400 kg/ha/yr, 2000 kg/ha/yr and 4000 kg/ha/yr respectively. Wheat bran, rice bran and mustard oil cake were given daily as an artificial feed in treatment I. Whereas treatment II was conducted without any artificial feed. Ratio of artificial feed was wheat bran:rice bran:oil cake=2:2:1 (by wt). Absence of artificial feed in 3 ponds under treatment II seriously affected the growth and production of fish.

Biodiversity in floodplains with special reference to artificial stocking

A five years investigation on fish biodiversity in connection with artificial stocking was conducted in three south-western floodplains of Bangladesh from 1992 to 1996. The ten top most available and ten rarest fish species were identified. Puntius sp., Channa punctatus, Mystus sp., Anabus testudinius, Ambasis sp., Colisha sp. and Macrobrachium sp. etc. were the most common available species. On the other hand, Mystus aor, Notopterus chitala, Clupisoma garua, Aplocheilus panchax, Ctenophmyngodon idella etc. were the rarest species. However, the most abundant and the rarest fish species behaved differently in different floodplains in different years. Shannon diversity index was used to assess the extent of diversity in different years. The study revealed that the artificial stocking programme, to some extent, influenced the biodiversity in floodplains.

Artificial insemination in penaeid shrimps

Artificial Insemination (AI) is a tool for genetic manipulation in the shrimp stocks. It is seen as one of the means for propagating shrimp culture to new areas by controlled reproduction. Attempts at artificial insemination in the dominant closed-thelycum penaeid shrimps species of the area viz. Metapenaeus affinis and Metapenaeus brevicornis were induced in wild adult stocks collected off Mumbai coast. Female specimens were subjected to unilateral eyestalk ablation by pinching so as to induce moulting and maturation. AI was performed two days after moulting on these females when the cuticle was still soft and flexible. Moulting also ensured rejection of initial spermatophores, if present. Response of males to electrical stimulation for spermatophore expulsion was spontaneous. Use of tissue glue for spermatophore retention was found to be unnecessary. Latency period ranged between10-16 days, while spawning occurred within 10-12 days of spermatophore transfer. Three partial spawning were recorded viz., two in Metapenaeus affinis and one in Metapenaeus brevicornis with an average spawning and hatching rates of 30% and 72.3% respectively. Average survival from first nauplius (N1) to one-day old post-larva (PLI) was a meager 3.43%. Use of AI in genetic manipulation of shrimp stocks for aquacultural purposes is indicated.

Magnetic field and current distributins in melt-textured YBa 2Cu 3O 7-x with an artificial grain boundary

Using a magneto-optical (MO) technique, magnetic field distributions have been measured in a melt-textured YBa 2Cu 3O 7-x bulk superconductor, joined to form an artificial grain boundary (GB), in an external magnetic field perpendicular to the sample surface. The magnetic field at a weak section of the GB shows different values between the field increasing up to 150mT and decreasing down to 0T after zero-field-cooling. Namely, the magnetic field in increasing field is higher than that in decreasing field, even in the same external field. This result supports a model in which such differences in magnetic field at the weak-link GB give rise to the hysteresis behavior in the field dependence of transport critical current density in polycrystalline samples. The field distributions across a well-joined region of the GB behave similarly to the adjoining bulk material and this result indicates the possibility of creating useful artifacts provided that the strongly coupled sections can be reproduced on a larger scale.

Variability of nanoindentation responses of bone and artificial bone-like composites

Nanoindentation is a popular technique for measuring the intrinsic mechanical response of bone and has been used to measure a single-valued elastic modulus. However, bone is a composite material with 20-80 nm hydroxyapatite plates embedded in a collagen matrix, and modern instrumentation allows for measurements at these small length scales. The present study examines the indentation response of bone and artificial gelatin-apatite nanocomposite materials across three orders of magnitude of lengthscale, from nanometers to micrometers, to isolate the composite phase contributions to the overall response. The load-displacement responses were variable and deviated from the quadratic response of homogeneous materials at small depths. The distribution of apparent elastic modulus values narrowed substantially with increasing indentation load. Indentation of particulate nanocomposites was simulated using finite element analysis. Modeling results replicated the convergence in effective modulus seen in the experiments. It appears that the apatite particles are acting as the continuous ("matrix") phase in bone and nanocomposites. Copyright © 2004 by ASME.

Probiotics and nutrient supplement in artificial feed of gold fish (Carassius auratus)

The efficacy of three feeds incorporated with different probiotics -Lactobacil, Vizylac and Cyfolac as nutrient supplement was evaluated in an ornamental fish, Carassius auratus (Linn.). The basal diet (40% protein) was prepared and the probiotics were incorporated at different levels viz. Lactobacil at 8x10 super(7)/100g and 12x10 super(7)/100g, Vizylac at 8x10 super(7)/100g and 12x10 super(7)/100g and Cyfolac at 12x10 super(7)/100g. Feeding trial was conducted for a period of 4 weeks. At the end of the experiment, bio-growth parameters and proximate composition of the fishes were studied. The growth, survival and protein content were improved in all the probiotic fed fishes compared to control. The maximum growth (0.74 g) and survival (85%) were observed in fishes fed with Lactobacil at 12x10 super(7)/100g. It emphasizes that supplementation of feed with probiotics has a positive impact on the growth, survival and the body composition of goldfish.