Evolution of the black muntjac (Muntiacus crinifrons) karyotype revealed by comparative chromosome painting

The black muntjac (Muntiacus crinifrons) has an unusual karyotype of 2n = 8 in females and 2n = 9 in males. We have studied the evolution of this karyotype by hybridising chromosome-specific paints derived from flow-sorted chromosomes of the Chinese muntjac (M. reevesi, 2n = 46) to chromosomes of the black muntjac. The hybridisation pattern allowed us to infer chromosomal homologies between these two species. Tandem and centromeric fusions, reciprocal translocations, and insertions are involved in the reduction of the diploid number from 2n = 46 to 2n = 8, 9. The painting patterns further show complex chromosomal rearrangements in the male black muntjac which involve more than half the karyotype, including both sex chromosomes. Since early meiosis is reported to be normal without any visible inversion loops of the synaptonemal complex, the observed chromosomal rearrangements would lead to heterosynapsis and, therefore, leave a large fraction of the male black muntjac karyotype balanced between the two sexes.

Reproductive biology, artificial propagation and larval rearing of two freshwater eels, Monopterus cuchia and Mastacembelus armatus

Studies on reproductive biology and artificial propagation including larval rearing of freshwater mud eel, Monopterus cuchia and spiny eel, Mastacembelus armatus were attempted. The gonadosomatic index (GSI) of mud eel ranged from 0.41 (August) to 5.52 (June) in males and 0.53 (August) to 7.61 (June) in females. In both cases the GSI showed a peak in June. Fecundity ranged from 228 (TL - 396 mm; W - 78g) to 5510 (TL - 865 mm; W - 630 g). In case of spiny eel, the GSI varied from 0.65 (August) to 8.30 (July) in males and 0.70 (August) to 10.46 (July) in females. GSI showed single peak in July. Fecundity ranged from 570 (TL - 240 mm; W - 30 g) to 10870 (TL - 601; W - 350g). Histology of the testes and ovaries of the eels were carried out to investigate the gonadal development stages during the reproductive months (August to November 2003). In case of male M. cuchia, the secondary primordial germ cells, primary spermatogonium, some spermatogonia A and clone of spermatogonium B in testis were observed in September. In October-males different sized lobules having spermatogonia, spermatocytes and spermatids were observed. In the ovary of M. cuchia, polygonal shaped oocytes were seen during September. The oogonia were reduced with dense and irregular shaped during October. Numerous pycnotic cells were visible during November. In male M. armatus numerous broken lobule walls were found in testes during September. In October, abundant primary germ cells, pycnotic nests of degenerating cells, spermatogonia and spermatids were observed. In females, ovaries had distinct yolk vesicles stage and yolk granules stages in August. In September, the follicular cells of the oogonia were ruptured, shrunk forming irregular shaped in October. Oogonia were also shrunk with thin, irregular shaped structure but broken parts of the ruptured follicular cells were scattered in case of M. armatus. Experimental attempts on artificial propagation indicated that both freshwater eels were difficult to breed using inducing agents like pituitary glands (PG) of 10, 20, 50, 100 and 150 mg per kg of body weight. Same doses were used for both sexes with equal sex-ratio. In both cases, brood fish died at higher doses of injection given at 100 and 150 mg PG/kg bodyweight. However, M. cuchia breed naturally in cisterns when provided with water hyacinths and tunnel in muddy bottom. M. cuchia fed with chopped cooked fish attained a mean weight of 18.75 ± 2.3 g and cent percent survival. While in case of M. armatus best growth by weight (12.0 ± 2.48 g) and cent percent survival were achieved using chopped raw fish. Car tyre was observed as best shelter for attaining the mean weight gain 22.53 ± 2.24 g and cent percent survival of M. cuchia. While PVC pipe was found to be the best shelter for M. armatus, where it attained the mean weight of 12.73 ± 1.88 g and cent percent survival.

Determination of length-weight relationship of squid (Loligo duvauceli) found along Pakistan coast

Common squid (Loligo duvauceli) is caught as by-catch of shrimp trawlers in shallow coastal. waters off Pakistan. Size frequency data of squid for sexes combined collected from Karachi Fish Harbour were analyzed. The length-weight relationship of the form W = a.L b was determined and to mean length of squid sample measured compared with mean length derived from inverse equation was tested for any significant differences, none were observed and it was inferred that the equation W = 0.243xL 2.2424 describe the relationship.

Seasonal and sex related haematological variations of the freshwater spiny eel, Mastacembelus armatus reared in the cemented cisterns

The seasonal and sex related haematological vanatlons in the freshwater spiny eel Mastacembelus armatuswere studied during summer from April to June 2001 and winter from November 2001 to January 2002. In total 60 fish (30 males and 30 females) ranging from 30- 55.6 cm in total length and from 55-95.5 g in weight were collected from the culture stock maintained in the cemented cisterns. It was observed that the values of the total erythrocyte count (TEC), haemoglobin concentration (Hb ), haematocrit values, packed cell volume (PCV) and neutrophil count were significantly higher (P< 0.05) in males than the females during summer (April to June- on-set of breeding season). Females were observed to have remarkably higher values (P< 0.01) of erythrocyte sedimentation rate (ESR), total leucocyte count (TLC) and thrombocyte count during summer. On the other hand, insignificant variations in the various haematological parameters were noticed during winter (November to January) between the two sexes of M. armatus.

Natural diet of penaeid prawns in the coastal waters of Mumbai

Foregut contents of eight commercially important species of penaied prawns namely Penaeus merguiensis, Metapenaeus affinis, M. monoceros, M. brevicornis, Parapenaeopsis stylifera, P. hardwickii, P. sculptilis and Solenocera crassicornis were investigated from inshore, nearshore and offshore fishing grounds of Mumbai. Feeding intensity and index of preponderance (IP) of the dietary items were compared statistically for the species, sexes, fishing areas and maturity condition of females. All the species except M. monoceros and P. sculptilis showed that females were better fed than males. The feeding intensity in the three depth-zones was different for M. affinis, M. brevicornis, M. monoceros, P. hardwickii and S. crassicornis, and uniform for P. merguiensis, P. stylifera and P. sculptilis. Acetes spp., prawn remains, polychaetes, benthic crustaceans, foraminifers and fish remains were the important food items of the prawns. Dietary comparison between the two sexes of the species did not show any difference, but mature females of M. monoceros and P. sculptilis had different diets. Comparison of food items for all the species together showed significant difference between the three areas. Crustacean diet was the favorite in the inshore and nearshore, and polychaetes in the offshore waters. All the species except P. hardwickii showed difference in their dietary composition in the three depth-zones. It is concluded that these coexisting species are primarily carnivorous and exhibit diverse food preferences in different depth-zones by browsing on interstitial organisms, chasing epipelagic prey, raptorial predation, scavenging on dead organisms or adopting different temporal abundance to avoid inter-specific competition for food.

Suggestive role of eye-stalk of Macrobrachium gangeticum Bate, 1868 in regulation of its reproductive behaviour

Role of eye-stalk of Macrobrachium gangeticum Bate, 1868 in its reproductive behaviour has been examined by conducting deletion and addition experiments. Eye-stalk ablation induced gonadal maturity in both sexes, leading to change in colour and size of ovaries and increase in GSI and oocyte diameter in females and increased length of testes and diameters of seminiferous tubules in males. Injection of eye-stalk extracts tended to at least partly restrict the effects in both sexes. The experiments thus suggested that the eye-stalk of M. gangeticum released some gonad inhibiting factors.

On certain allometric relations of the spiny lobster Panulirus polyphagus (Herbst)

Certain allometric relations of the spiny lobster Panulirus polyphagus are derived. While the relation between tail length and tail weight is made for both the sexes together, separate relations are derived for the sexes in the case of tail length versus total length. For conservation and economic purposes, it appears that the undersized ones are to be released alive.

Fat and water contents of the muscle and gonad of Otolithus argenteus in relation to gonad growth

Fat and water contents of the muscles and gonads in relation to gonad growth in Otolithus argentes from Karwar are reported. Water content showed an inverse relationship to lipid in both the sexes. Variation in somatic body weight was found associated with the fluctuations in lipid contents.

Distribution of 5'-nucleotidase activity in the tissues of a freshwater teleost, Ophicephalus striatus Bloch

Homogenates of tissues have been analysed for the presence of 5'-nucleotidase activity. Sexes are not treated individually since no significant differences were observed. All organs showed marked activity, the highest being in the cardiac tissues, then the kidney, spleen, brain, and low values in the liver and muscles. Comparison with data from other fish studied shows a marked difference between O. striatus and O. punctatus.

Length-weight and tail length-total length relationship in Panulirus homarus Linnaeus on the south-west coast of India

Length-weight relationships of Panulirus homarus caught from the Kanniyakumari coast was found to be W=0.000566 L super(2-50). The relationship between tail length and total length was also investigated and compared with that of Panulirus polyphagus. Same relationship did not hold good for the two sexes as in the case of P. polyphagus. For a given tail length, the head length, the total length and the weight appear to be relatively larger for females of small sizes of P. homarus.

Ecology and life history of penaeid shrimps

Fourteen species of penaeid shrimps with commercial value in Batan Bay and Tigbauan-Guimbal waters were identified as follows: Penaeus monodon, P.semisulcatus, P.japonicus, Metapenaeus ensis, M.burkenroadi, M.endeavouri, Metapenaeopsis palmensis, M.stridulans, Trachypenaeus fulvus, and Parapenaeus longipes. Among the 14 penaeids, P.semisulcatus, M.ensis and M.palmensis were found to be the dominant species within each genus. There are seven existing fishing gears for shrimping in the Batan Bay and Tigbauan-Guimbal waters: fish corrals, lift net, filter net, gill net, skimming net, baby trawler and commercial trawler. In general, female penaeids are larger than males. The largest P.monodon female measured was 81 mm in carapace length with 23 g in body weight. The largest male measuring 59 mm in carapace length with 119 g of body weight was caught in Batan Bay. Judging from spermatozoa occurrence on both sexes of P.monodon, the biological minimum size for male is 37 mm in carapace length and 49 mm for female. A total of 133 Penaeus postlarvae obtained from the offshore were identified by comparison with those reared in the laboratory. The postlarvae of P.japonicus-latisulcatus complex were quite dominant (60 . 2%), followed by P.semisulcatus (18 . 0%), and P.merguiensis-indicus complex (17 . 3%). The number of P.monodon postlarvae was relatively small (4 . 5%). The modal carapace length of P.monodon postlarvae from the offshore was 1 . 3 mm with three or four dorsal and no ventral spines on the rostrum, while P.monodon fry from the shoreline had 2 . 3 mm with five or six dorsal and one or two ventral spines.

Notes on the external sex characters of Chanos chanos Forskal spawners

In the present study, no visible differences between the sexes of C. chanos with reference to external features such as colouration, shape of head, snout and operculum, presence of tubercles or nasal pores, length, size and shape as well as any roughness in the various fins, could be found. However, the anal region of the mature milkfish (sabalo) exhibits discernible anatomical differences in the male and female. The male has two main openings visible externally: the anterior anus and the posterior urogenital opening at the tip of the urogenital papilla. The female has three main openings instead of two: the anteriormost anus, followed by the genital pore and the urinary pore located posterior to the genital pore at the tip of the urogenital papilla. Internal examinations were also made on both sexes. In ripe sabalo, it is easier to distinguish the sexes since milk oozes out of the urogenital pore by pressing the abdomen of the ripe male fish. Gravid females are identified by their distended abdomens.

Length-weight relationship of two palaemonid prawns, Macrobrachium idella and M. scabriculum: a comparative study

Length-weight relationships of two commercially important freshwater prawns, Macrobrachium idella and M. scabriculum, have been worked out separately for the two sexes of the two species. 't' test showed that growth departs significantly from the isometric growth in the case of both sexes of M. idella and in the female of M. scabriculum. Therefore, the cubic formula W = CL^3 may be applied only in the case of males of M. scabriculum. In all the other cases W = CL^n may be followed.

Length-weight relationship in Nandus nandus (Hamilton) (Perciformes-Teleostei)

Length-weight relationship of the freshwater fish of Pamba River, Nandus nandus (Ham) has been worked out. The results showed that the slope values and elevations were not significant and hence a combined regression equation has been calculated for both the sexes (Log W=2.4130 Log L -0.3306). The’t’ test analyses were conducted and found that the growth departs significantly from the isometric growth. Thus the formula W=aL super(n) has to be applied in calculating the length-weight relationship of this species.

The study of maturational progress in the whitespotted rabbitfish (Siganus sutor) under different photoperiod and light intensity conditions

The rule of light on the timing of maturation and spawning in tropical and subtropical regions is not clear well, because the reproductive cycle in these systems is lunar synchronized. In this study, thus, the effects of different light regimes were investigated on maturational progress of whitespotted rabbitfish, Siganus sutor, the commercial species in Persian Gulf and Oman Sea. During prespawning season, 50 adult fish were randomly divided into ten 300-l tanks (n=5). The fish in control tank received indoor light condition and the fish in each other tanks were exposed to nine different combinations of photoperiod (8L: 16D, 12L: 12D, 16L: 8D) and light intensity (1000, 2000, 3000 lux). After 60 days, GSI and HSI values, serum levels of estradiol (E2), testosterone (T), 17-α hydroxyprogestrone (OHP), calcium (Ca2+) and gonad histology were evaluated for females and males. In females the GSI mean values of exposed fish increased in comparison with control except for fish were kept under 8L, 2000 lux (tank 8). These differences were significant only for fishes in tank 7 (8L, 3000 lux). In the cases of HSI, the results were converse, so that, the most of thanks showed significant decreasing than fishes reared under indoor condition. Morphology and histology study of Ovaries showed three developmental phases including 3, 4 and chiefly 5 that were parallel with GSI values. Fortheremore the serum levels of E2 was recorded between 0.54-15.8 ng/ml in different fish and their mean values were lower than control in all treatmants (P> 0.05). In males, the similar results were obtained. The GSI and HSI mean values in experimental regimes compared with control were upper and lower, respectively, except for fishes were reared in tank 1 (16L, 3000 lux). Testes histology of fishes were reared under different regimes showed signs of stage 5, since no blood vessels observed and thick milt exuded on slight pressure. The mean values of testosterone consentration in fishes were kept in tanks 1 and 6 (12L, 1000 lux) were higher and in other ones were lower than control group. It is also noted that the OHP and Ca2+ had diverse results including increasing and decreasing mean values than control. So, these factors contrary to E2 and T were not suitable to evaluation of maturity in both sexes. On the basis of ovarian structure in stage 5, oocyte development pattern in this species was group synchronous. So, increased mean of GSI versus decreased values of HSI, E2 and perhaps Ca2+ were the signs of improved maturation. But in males, reduced levels of T and similarity of testes morphology in all samples caused that GSI mean value was the only indicator for differentiating among treatments. These findings suggest that alternations were used in light regimes have been the reason of improved maturity in all treatments except fishes reared in tank 8. The ١٠٧ rule of light intensity on induction of maturity was cleared by comparision between fishes in tanks 4, 5, 6 and control group. Because day length was the same whereas fishes in tanks 4, 5 and 6 were exposed to increased light intensity compared with control. This fact verified by results was obtained from fishes in tanks 9 and especially 7, since photoperiod in this group was lower than control. So, higher intensity was considered as the reason of alternations. Contrasting with indoor condition, Induced maturity was also cleared for fishes were kept in tanks 1, 2 and 3, where both of light duration and intensity were increased. But, the rule of photoperiod was individually demonstrated when obtained results were compared with similar light intensities in other treatments. In conclusion, with comparison among different light regimes it is declared that siganids were kept under light condition of tank 2 including 16h light duration combined with 2000 lux intensity showed the best signs of sexual maturation in both sexes. On the basis of this study, setting up the spring light condition during prespawning season induces maturation in withspotted rabbitfish. This improvement not only by influence of photoperiod or light intensity, separately, but obtained through interaction between them. Thus, determination of threshold and resistance to light be recommended before exposure, although using proper regime during suitable time are necessary to achieve purposes considerably.