Nucleator-dependent intercellular assembly of adhesive curli organelles in Escherichia coli.

Bacterial adhesion to other bacteria, to eukaryotic cells, and to extracellular matrix proteins is frequently mediated by cell surface-associated polymers (fimbriae) consisting of one or more subunit proteins. We have found that polymerization of curlin to fimbriae-like structures (curli) on the surface of Escherichia coli markedly differs from the prevailing model for fimbrial assembly in that it occurs extracellularly through a self-assembly process depending on a specific nucleator protein. The cell surface-bound nucleator primes the polymerization of curlin secreted by the nucleator-presenting cell or by adjacent cells. The addition of monomers to the growing filament seems to be driven by mass action and guided only by the diffusion gradient between the source of secreted monomer and the surface of monomer condensation.

Numerical evaluation of the vibration reduction index for structural joints

The present paper addresses the analysis of structural vibration transmission in the presence of structural joints. The problem is tackled from a numerical point of view, analyzing some scenarios by using finite element models. The numerical results obtained making use of this process are then compared with those evaluated using the EN 12354 standard vibration reduction index concept. It is shown that, even for the simplest cases, the behavior of a structural joint is complex and evidences the frequency dependence. Comparison with results obtained by empirical formulas reveals that those of the standards cannot accurately reproduce the expected behavior, and thus indicate that alternative complementary calculation procedures are required. A simple methodology to estimate the difference between numerical and standard predictions is here proposed allowing the calculation of an adaptation term that makes both approaches converge. This term was found to be solution-dependent, and thus should be evaluated for each structure.

A new approach for semi-automatic rock mass joints recognition from 3D point clouds

Rock mass characterization requires a deep geometric understanding of the discontinuity sets affecting rock exposures. Recent advances in Light Detection and Ranging (LiDAR) instrumentation currently allow quick and accurate 3D data acquisition, yielding on the development of new methodologies for the automatic characterization of rock mass discontinuities. This paper presents a methodology for the identification and analysis of flat surfaces outcropping in a rocky slope using the 3D data obtained with LiDAR. This method identifies and defines the algebraic equations of the different planes of the rock slope surface by applying an analysis based on a neighbouring points coplanarity test, finding principal orientations by Kernel Density Estimation and identifying clusters by the Density-Based Scan Algorithm with Noise. Different sources of information —synthetic and 3D scanned data— were employed, performing a complete sensitivity analysis of the parameters in order to identify the optimal value of the variables of the proposed method. In addition, raw source files and obtained results are freely provided in order to allow to a more straightforward method comparison aiming to a more reproducible research.

The pastrycook & confectioner's guide : for hotels, restaurants, and the trade in general adapted also for family use : including a large variety of modern recipes for bread, cakes, fancy biscuits, ice creams and water ices, jellies, pies, puddings and custards, joints, meat pies and dishes, poultry and game, ornamental sugar work & butter-work etc. : with useful hints and instructions /

"To build an ice-house": p. [57]-58.

Modern British permanent way. Treating of rails, chairs, fishbolts, fishplates, keys, sleepers, ballast, rail-joints, points and crossings, &c.

"Originally written as a series of articles for ʻThe Railway Newsʾ, in which they appeared at various periods between the years 1911 and 1914."--Pref.

Survey and correlation report on continuously reinforced pavements without joints, by W. H. Jacobs.

Mode of access: Internet.

Fatigue tests of plate joints and bolted lugs. ARO project. Final report, September 30, 1954.

Mode of access: Internet.

Studies of the fatigue behavior of butt-welded joints in HY-80 and HY-100 steels /

"AD no. 452191."

American adhesive index.

Bibliography: p. 149.

Liquid rocket disconnects, couplings, fittings, fixed joints, and seals /

Cover title.

A treatise on the theory and management of ulcers : with a dissertation on white swellings of the joints. To which is prefixed an essay on the chirurgical treatment of inflammation and its consequences /

Mode of access: Internet.

An account of a new method of treating diseases of the joints of the knee and elbow : : in a letter to Mr. Percival Pott /

Mode of access: Internet.

Adhesive bonding of titanium and its alloys /

"August 1965."

Methods of preparing horizontal construction joints in mass concrete /

Mode of access: Internet.