Characterization of a unique variant of bat rabies virus responsible for newly emerging human cases in North America.

The silver-haired bat variant of rabies virus (SHBRV) has been identified as the etiological agent of a number of recent human rabies cases in the United States that are unusual in not having been associated with any known history of conventional exposure. Comparison of the different biological and biochemical properties of isolates of this virus with those of a coyote street rabies virus (COSRV) revealed that there are unique features associated with SHBRV. In vitro studies showed that, while the susceptibility of neuroblastoma cells to infection by both viruses was similar, the infectivity of SHBRV was much higher than that of COSRV in fibroblasts (BHK-21) and epithelial cells (MA-104), particularly when these cells were kept at 34 degrees C. At this temperature, low pH-dependent fusion and cell-to-cell spread of virus is seen in BHK-21 cells infected with SHBRV but not with COSRV. It appears that SHBRV may possess an unique cellular tropism and the ability to replicate at lower temperature, allowing a more effective local replication in the dermis. This hypothesis is supported by in vivo results which showed that while SHBRV is less neurovirulent than COSRV when administered via the intramuscular or intranasal routes, both viruses are equally neuroinvasive if injected intracranially or intradermally. Consistent with the above findings, the amino acid sequences of the glycoproteins of SHBRV and COSRV were found to have substantial differences, particularly in the region that contains the putative toxic loop, which are reflected in marked differences in their antigenic composition. Nevertheless, an experimental rabies vaccine based on the Pittman Moore vaccine strain protected mice equally well from lethal doses of SHBRV and COSRV, suggesting that currently used vaccines should be effective in the postexposure prophylaxis of rabies due to SHBRV.

A pre-Columbian Y chromosome-specific transition and its implications for human evolutionary history.

A polymorphic C-->T transition located on the human Y chromosome was found by the systematic comparative sequencing of Y-specific sequence-tagged sites by denaturing high-performance liquid chromatography. The results of genotyping representative global indigenous populations indicate that the locus is polymorphic exclusively within the Western Hemisphere. The pre-Columbian T allele occurs at > 90% frequency within the native South and Central American populations examined, while its occurrence in North America is approximately 50%. Concomitant genotyping at the polymorphic tetranucleotide microsatellite DYS19 locus revealed that the C-->T mutation displayed significant linkage disequilibrium with the 186-bp allele. The data suggest a single origin of linguistically diverse native Americans with subsequent haplotype differentiation within radiating indigenous populations as well as post-Columbian European and African gene flow. The mutation may have originated either in North America at a very early time during the expansion or before it, in the ancestral population(s) from which all Americans may have originated. The analysis of linkage of the DYS199 and the DYS19 tetranucleotide loci suggests that the C-->T mutation may have occurred around 30,000 years ago. We estimate the nucleotide diversity over 4.2 kb of the nonrecombining portion of the Y chromosome to be 0.00014. compared to autosomes, the majority of variation is due to the smaller effective population size of the Y chromosome rather than selective sweeps. There begins to emerge a pattern of pronounced geographical localization of Y-specific nucleotide substitution polymorphisms.

‘Quite destitute and … very desirous of going to North America’ : The Roots and Repercussions of Emigration from Sutherland and Caithness

Peer reviewed

Women in industry: a study in American economic history

by Edith Abbott.

Iniopterygia : a new order of Chondrichthyan fishes from the Pennsylvanian of North America / Rainer Zangerl [and] Gerard R. Case.

v.6(1973)

A seriation of the late prehistoric Santa Maria culture of northwestern Argentina / Ronald L. Weber, Field Museum of Natural History.

v.68:no.2(1968)

Material culture of the Chilcotin Athapaskans of west central British Columbia : collections in the Field Museum of Natural History / James W. VanStone.

n.s. no.20(1993)

Ziphodont crocodiles, Pristichampsus vorax (Troxell), new combination, from the Eocene of North America / Wann Langston, Jr. --.

v.33:no.16(1975)

Catalog of type and referred specimens of fossil ostracodes in the Field Museum of Natural History / [by] Matthew H. Nitecki -- and Diana Z. Handler --.

v.17:no.5(1968)

Ethnographic collections from the Assiniboine and Yanktonai Sioux in the Field Museum of Natural History / James W. VanStone.

n.s. no.26(1996)

Killke and Killke-related pottery from Cuzco, Peru, in the Field Museum of Natural History / Brian S. Bauer, Charles Stanish.

n.s. no.15(1990)

Traditions of the Arapaho, collected under the auspices of the Field Columbian museum and of the American museum of natural history, by George A. Dorsey -- and Alfred L. Kroeber --

v.5(1903)

"And he was beautiful" : contemporary Athapaskan material culture in the collections of Field Museum of Natural History / William E. Simeone, James W. VanStone.

n.s. no.10(1986)