Detection of Paracoccidioides spp. in environmental aerosol samples

Taking into account that paracoccidioidomycosis infection occurs by inhalation of the asexual conidia produced by Paracoccidioides spp. in its saprobic phase, this work presents the collection of aerosol samples as an option for environmental detection of this pathogen, by positioning a cyclonic air sampler at the entrance of armadillo burrows. Methods included direct culture, extinction technique culture and Nested PCR of the rRNA coding sequence, comprising the ITS1-5.8S-ITS2 region. In addition, we evaluated one armadillo (Dasypus novemcinctus) as a positive control for the studied area. Although the pathogen could not be isolated by the culturing strategies, the aerosol sampling associated with molecular detection through Nested PCR proved the best method for discovering Paracoccidioides spp. in the environment. Most of the ITS sequences obtained in this investigation proved to be highly similar with the homologous sequences of Paracoccidioides lutzii from the GenBank database, suggesting that this Paracoccidioides species may not be exclusive to mid-western Brazil as proposed so far. © 2013 ISHAM.

Estimativa das radiações ultravioleta (UV), fotossintéticamente ativa (PAR) e infravermelha (IV) em função da razão de insolação

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Sítios assistenciais: modelo de organização e classificação de pacientes em UTI

Pós-graduação em Enfermagem (mestrado profissional) - FMB

Avaliação de variáveis ambientais em ambientes destinados a ocupação comum

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Carbono orgânico solúvel em água no material particulado de regiões canavieiras do Estado de São Paulo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação de sistemas para a aplicação de inseticidas para controle de Aedes aegypti (l.) (Diptera: Culicidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Ocorrência de micobactérias e outros microorganismos nas águas de uma fazenda produtora de leite de búfalas, na região de São Carlos, estado de São Paulo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efetividade da irradiação por micro-ondas e da terapia fotodinâmica na inativação de micro-organismos presentes em próteses totais: estudo clínico

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Propriedades químicas e físicas do material particulado atmosférico e seus efeitos no crescimento de partículas

Pós-graduação em Química - IQ

Proposta de protocolo admissional à equipe de enfermagem sobre o uso de medidas de precaução de contato

Pós-graduação em Enfermagem (mestrado profissional) - FMB

Imagens de satélite na análise da relação entre queimadas, aerossóis atmosféricos e precipitação

The direct and indirect effects of atmospheric aerossols in the amazonic climate have been focus of many published. This work aims to analyze a possible relation among Burns, atmospheric aerossols and precipitation above South America. It uses the avaluable data from the satellites AQUA/TERRA and TRMM and the images furnished by NOAA and GOES. These analysis can provide some explanations about the effects of emission of aerosols by burns on the composition of atmosphere and cloud formation, mainly in Amazon. We use the Aerosol Optical Depth (AOD) from MODIS/TERRA-AQUA, burns index from CPTEC and rain rate from TRMM. The data we use were obtained from 2000 until 2012. The study is divided in two parts. In the first one, it was performed a quantitative analysis between number of burns and aerosols emission. It was identified a great variability in space and time of the AOD on South America. On the north, northeast and center-west, the AOD is significant during the winter period, with peak on August and September. The southeast is affected by aerosols from center-west due to the dynamical transport. In the second part, it was evaluated the relation between AOD and precipitation in a 13-year period. The statistical analysis shows up a negative correlation of 0.72 between August and October, on Legal Amazon. These result indicate an inverse relation between AOD and rain rate. The other months present not significant correlation. These results are in a good agreement with the literature, in which in-situ methods were applied or combined with satellites data. The increasing of aerosols concentration in the atmosphere are reinforced during drier years. It can affect the increasing process of water drops, decreasing the precipitation. We also verified higher values of AOD (0.25 - 0.3) during years with El Niño, than the climatologically average (~0.15 - 0.2), ... (Complete abstract click electronic access below)

Diurnal and annual evolution of UV, PAR and NIR irradiations in Botucatu/Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Biossegurança na clínica de radiologia odontológica

In the practice of dental radiology, although there was no production of aerosols, there is the contamination of equipment and surfaces by contact with blood and saliva. This is making necessary the adoption infection control procedures to protect patients and staff. The objective of this paper is to present the procedures of prevention during the radiographic examination and radiographic processing in the darkroom. The proposed recommendations for the area of Dental Radiology include efficient cleaning and protection of equipment and surfaces, personal protective measures and to avoid contamination of the darkroom. These recommendations are obtained by simple procedures as cover films and radiological equipment with plastic barriers.

Raman Lidar monitors emissions from sugar cane fires in the State of São Paulo: a pilot-project integrating radar, sodar, aerosol and gas observations

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo da distribuição de tamanho e composição iônica de aerossóis e seus efeitos na capacidade de nuclear gotas de nuvens

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)