Avaliação gastroprotetora da epicatequina e verificação de seus mecanismos de ação

The stomach is an exceptional organ, which functions are sterilize food ingested, form the primitive bolus, digest lipids and proteins, and to store food temporarily in the gastrointestinal tract. Its capacity of digesting food without digesting itself is amazing. This fact occurs due to innumerous protective substances adjacent to the gastric mucosa. When aggressive factors overwhelm the protective factors, a lesion in the gastric mucosa is formed. Lesions that reach the lamina propria are called gastric ulcers, which are classified macroscopically as openings on the gastric wall and; microscopically, as a gastric injury characterized with epithelial desquamation, mucosal hemorrhage, glandular damage and eosinophilic infiltration. The current therapy available is effective, although it causes collateral effects, therefore researching new drugs is necessary. This work aim to evaluate the gastroprotective effect of epicatechin against gastric lesions induced by absolute ethanol and non steroidal anti-inflammatory drugs which are the main causes of this disease currently, yet we aim to study the main mechanisms of action responsible for the gastroprotective effect. The results show that epicatechin has a significant macroscopic and microscopic gastroprotective effect against gastric injuries induced by ethanol and indomethacin, acting locally by augmenting gastric mucus secretion and it also acts via antioxidant system by holding total glutathione levels. Epicatechin’s gastroprotective mechanisms depend on the activation of sulfhydryl compounds and doesn’t depend on the NO-synthase enzyme

Jejum no pré-abate de suínos: bem estar animal e qualidade da carne

In the pre-slaughter management of pigs there are stressors, which depending on the duration or severity, are able to alter the quality of meat. One of the factors considered critical to the welfare is the fasting period in the farm. This practice is fundamental and proven relevance in the production chain. Besides the effects on meat quality and welfare of pigs, fasting is among the main factors responsible for the incidence of skin lesions, can significantly influence the incidence of esophagogastric ulcers and is also important to avoid any contamination of carcasses by gastrointestinal contents. When fasting is done right, it has a positive impact on welfare, meat quality and conditions of welfare during the pre-slaughter management of pigs

Expressão de matriz metaloproteinases e PCNA me úlceras corneais profundas, induzidas em coelhos, tratadas com plasma rico em plaquetas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Uso de plasma rico em plaquetas em úlceras de córnea em cães

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Ketorolac eye drops reduce inflammation and delay re-epithelization in response to corneal alkali burn in rabbits, without affecting iNOS or MMP-9

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Aplicação do sistema de biosusceptometria AC para monitoramento de nanopartículas magnéticas na circulação sanguínea

Pós-graduação em Biologia Geral e Aplicada - IBB

Avaliação das atividades cicatrizante e antiinflamatória de Casearia sylvestris Swartz em animais e identificação dos marcadores químicos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Expressão gênica de TLR-2, TLR-4, HMGB1 E VEGF em úlceras abomasais em bovinos de corte

Pós-graduação em Ciência Animal - FMVA

Anestesia peribulbar para enucleação em um felino: relato de caso

Foi atendido no Hospital Veterinário “Luiz Quintiliano de Oliveira”, o felino Nick, SRD, de quatro meses de idade, pesando 700 gramas, com histórico de trauma por arranhadura em bulbo ocular esquerdo. Ao exame físico específico, evidenciou-se úlcera profunda e prolapso de íris, sendo então indicada a enucleação. Ao exame físico geral, observou-se freqüência cardíaca (FC) de 160 batimentos.min-1, frequência respiratória (f) de 80 movimentos.min-1, tempo de preenchimento capilar menor do que dois segundos, temperatura retal (TR) de 39,2oC e mucosas normocoradas. Como medicação pré-anestésica, empregou-se acepromazina (0,04 mg.kg-1) e metadona (0,3 mg.kg-1), administradas pela via intramuscular. A veia femoral esquerda foi cateterizada com cateter 24G para administração de Ringer com Lactato de sódio (10 mL.kg-1.h- 1 ). A indução foi realizada por máscara facial tendo-se como agente o isofluorano em fluxo diluente de 100 mL.kg-1.min-1 de oxigênio a 100%, seguida de intubação orotraqueal com sonda n o 2,5 sem cuff. Seguiu-se a manutenção anestésica com a mesma mistura da indução, administrada por meio de circuito anestésico sem reinalação de gases, do tipo Baraka, mantendose o paciente sob ventilação assistida. Ato contínuo, realizou-se a técnica anestésica peribulbar de punção única inferior, utilizando-se lidocaína 2% com vasoconstritor (3mg.kg-1) associada a bupivacaína 0,5% sem vasoconstritor (0,8mg.kg-1), perfazendo um volume total de 0,3ml.kg-1 . Uma agulha 13x4,5 foi introduzida em todo o seu comprimento com o bisel voltado para a órbita, no terço lateral do fórnice conjuntival inferior da órbita esquerda, administrando-se a associação dos agentes anestésicos locais, seguida de compressão manual da área para facilitar a difusão dos mesmos. Durante o procedimento anestésico, realizou-se a monitoração da FC, f, pressão arterial sistólica (PAS), TR e saturação periférica da hemoglobina (SpO2). O tempo total de anestesia e cirurgia foi de 30 e 20 minutos, respectivamente, e a SpO2, concentração de isofluorano e TR mantiveram-se em 99±1%, 1,7±0,8% e 37,4±1,5oC, respectivamente. O plano anestésico manteve-se estável, sem a necessidade de resgate analgésico. Não houve a ocorrência de reflexo óculo-cardíaco (ROC) frente à manipulação do nervo óptico, o que pode ser atribuída provavelmente ao bloqueio peribulbar. A anestesia regional é frequentemente empregada para cirurgias oftálmicas em humanos, como a facoemulsificação, sendo que o manejo anestésico pode contribuir para o sucesso do procedimento. Pode-se concluir que, também na espécie felina, o bloqueio peribulbar pode ser uma boa alternativa para a realização de protocolos de anestesia balanceada para procedimentos oftálmicos.

Carcinoma de células escamosas de grandes proporções

The oral and oropharyngeal cancer is aggressive and, in Brazil, the incidence is considered one of the world’s tallest, the most common of head and neck. It affects males more intensively and 70% of cases are in adults over 50 years of age. It is located usually on the floor of the mouth and tongue. Therefore, the purpose of this study is to report a case of male patient, 78 years of age, leukoderma who sought hospital care. Reported frequent smoking and alcoholism. On physical examination headgear, there was the presence of lymph node fixed, painless 1.5 to 2 cm in diameter in the submandibular region. Physical examination intraoral ulcer was found at about 5 cm at its greatest extent, localized to the left oral floor region of the oropharynx, reddish, hardened edges, surface and bottom corrugated carton. The differential diagnosis suggested was traumatic ulcer, paracoccidioidomycosis and squamous cell carcinoma. The approach employed was obtained by incisional biopsy and the piece sent to histopathological analysis, confirming the case of squamous cell carcinoma. The treatment consisted in clarifying the patient about the disease and the need to search for an oncology center. It is concluded that the oral clinical examination is accurate in all major injuries to mouth, even if the chief complaint does not focus on this. In individuals at high risk examination should be systematic and individuals with suspicious lesions should be referred to specialist consultation.

Helicobacter pylori em boca: epidemiologia e associação com doenças bucais

The association of Helicobacter pylori with different gastric diseases has been continuously discussed in the literature, whereas the last 20 years a large body of publications was intended to characterize the ecological niches and habitats of this pathogen being the mouth a possible habitat for that rod. The present study aimed to discuss the occurrence of this organism as part of the supplemental or transient microbiota of the mouth. Thus, an extensive review of literature covering the period 1990-2014 was carried out with different databases, yielding 5024 articles discussing transmission and occurrence of H. pylori, in English or Portuguese. Articles were selected according to the inclusion and exclusion criteria and data were tabulated and analyzed. Despite the significant heterogeneity of the literature, it was found this microaerophilic has a worldwide distribution, even in the mouth, which behaves as a reservoir for gastric reinfection. The role of oral hygiene and periodontal disease in the distribution of this microorganism remains unclear, but most studies support the hypothesis that oral biofilms and inflammation of periodontium may facilitate the installation of this pathogen in the mouth. Studies suggest that this microorganism could collaborate as a predisposing factor for oral cancer.

A influência do anel na evolução ponderal após quatro anos da derivação gátrica em Y-de-Roux laparoscópica

Use of ring in Roux-en-Y gastric bypass is still a matter of controversy among bariatric surgeons. There is no consensus on its impact in relation to weight loss and weight maintenance in the long term. AIM: To evaluate the influence of the ring on the evolution of body weight over four years after bariatric surgery. METHODS: Retrospective analyzis of 143 women who underwent laparoscopic Roux-en-Y gastric bypass paired on the use or not use of Silastic® ring. Follow-up time was 48 months. Inclusion criteria were age over 18 years, primary bariatric operation and regular attendance at the clinic during the period of interest for research. The technique kept small gastric reservoir estimated in a volume of 30 ml. The food limb had in average 150 cm and the bile one 40 cm from the duodenojejunal angle. The group "ring" used Silastic® device with length of 6.5 cm, placed 2 cm from gastrojejunal anastomosis. The ring was closed for five polypropylene surgical thread sutures. In the morning after surgery the patients received isotonic fluids; on the second day salty liquid diet and were discharged on the third day. Semisolid diet started from the 20th day and solid on the 30th, with daily tablet of polivitamins. RESULTS: The weight loss was larger on the ring than without ring groups in all periods, respectively 10% and only 5% in the third postoperative year. The proportion of not having reached the 50% excess weight loss expectative was significantly higher in the group without ring than in the group with the ring (31% and 8% respectively in the fourth year). There was no difference between groups in delayed recovery of weight lost with the operation. CONCLUSIONS: The results were favorable to use the ring exclusively when it is analyzed only the weight loss.

Boas práticas de laboratório e biossegurança: prevenção dos riscos ergonômicos

El control de los riesgos ergonómicos es parte del paquete de medidas de Buenas Prácticas de Laboratorio y Bioseguridad. De este modo, la mala postura, la iluminación o ventilación inadecuadas , prolongada jornada de trabajo, la monotonía y la actividad repetitiva, intensa rutina, el control de la productividad , el estrés y el trabajo por la noche son factores a los riesgos ergonómicos. Como se relacionan a los elementos físicos y de organización también pueden interferir con la comodidad y la salud del personal de laboratorio. Riesgos ergonómicos no sólo pueden generar trastornos psicológicos y fisiológicos que causan graves daños a la salud, sino también comprometer la productividad del laboratorio y reducir el equipo de seguridad , ya que producen cambios en el cuerpo y el estado emocional, tales como trastornos o lesiones relacionadas con el cansancio físico producido por el trabajo repetitivo, dolor muscular, alteraciones del sueño, diabetes, trastornos de los nervios , la taquicardia , las enfermedades del aparato digestivo (gastritis y úlcera ), tensión, ansiedad, problemas de espalda y la hipertensión. En este trabajo se propone una secuencia de actividad laboral (gimnasia) en el intervalo de tiempo pequeño, respetando las instalaciones y el espacio físico disponible en el ambiente del trabajo, como una estrategia para mejorar la calidad de vida laboral, para aumentar la productividad, mejorar la disposición a trabajar y para aumentar el conocimiento del cuerpo y de la interacción social.

Polimorfismos genéticos como moduladores do consumo alimentar, peso corporal e comorbidades após um ano de cirurgia bariátrica

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Associação da suplementação de vitamina D3 e do alcoolismo experimental em ratos: efeitos morfológicos e comportamentais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)