Assembly of the Escherichia coli RuvABC resolvasome directs the orientation of holliday junction resolution.

Genetic recombination can lead to the formation of intermediates in which DNA molecules are linked by Holliday junctions. Movement of a junction along DNA, by a process known as branch migration, leads to heteroduplex formation, whereas resolution of a junction completes the recombination process. Holliday junctions can be resolved in either of two ways, yielding products in which there has, or has not, been an exchange of flanking markers. The ratio of these products is thought to be determined by the frequency with which the two isomeric forms (conformers) of the Holliday junction are cleaved. Recent studies with enzymes that process Holliday junctions in Escherichia coli, the RuvABC proteins, however, indicate that protein binding causes the junction to adopt an open square-planar configuration. Within such a structure, DNA isomerization can have little role in determining the orientation of resolution. To determine the role that junction-specific protein assembly has in determining resolution bias, a defined in vitro system was developed in which we were able to direct the assembly of the RuvABC resolvasome. We found that the bias toward resolution in one orientation or the other was determined simply by the way in which the Ruv proteins were positioned on the junction. Additionally, we provide evidence that supports current models on RuvABC action in which Holliday junction resolution occurs as the resolvasome promotes branch migration.

Iowa Department of Elder Affairs Strategic Plan, 2013-2014

This report outlines the strategic plan for Iowa Department of Elder Affairs, goals and mission.

Iowa Department for the Blind State Strategic Plan, July 1, 2011-June 30, 2014

The Iowa Department for the Blind is the means for persons who are blind to obtain for themselves universal accessibility and full participation as citizens in whatever roles they may choose, including roles that increase family income, create jobs, improve educational outcomes, and reduce reliance on public services.

Iowa Board of Parole Strategic Plan 2012-2013

This report outlines the strategic plan for Iowa Board of Parole Strategic Plan including,goals and mission.

Iowa Civil Rights Commission Strategic Plan 2012-2015

This report outlines the strategic plan for Iowa Civil Rights Commission Strategic Plan including,goals and mission.

Iowa Alcoholic Beverages Division, Strategic Plan, 2003 (adopted October 2003

This report outlines the strategic plan for Iowa Alcoholic Beverages Division including, goals and mission.

Iowa Credit Union Division, Strategic Plan, 2012

This report outlines the strategic plan for Credit Union Division including, goals and mission.

Iowa Division of Banking, Strategic Plan, 2012-2016

This report outlines the strategic plan for Iowa Division of banking including, goals and mission.

Iowa Insurance Division Strategic Plan, FY2013

This report outlines the strategic plan for Iowa Insurance Division including, goals and mission.

Iowa Department of Cultural Affairs, Strategic Plan 2012-2015

The Department of Cultural Affairs was created in the state government reorganization in 1986. The department’s two divisions, the Iowa Arts Council and the State Historical Society (SHSI) have a much longer history of service to Iowans. With such a rich history, it is fitting that the Department of Cultural Affairs calls the State Historical Museum home, a facility which collects, preserves and showcases Iowa’s treasures that itself can trace its roots in state government back 120 years. Generations of Iowans have been touched by the programs and services provided through this department. Though organized as one department, the DCA has never undergone strategic planning as a single, cohesive organization. With a desire to move forward as a stronger, more unified entity, a departmentwide strategic planning process becomes critical to future success as the department continues to better assess its resources and expertise internally, while ensuring it is focused on the common goal of leveraging cultural resources across the state of Iowa.

Iowa Department of Natural Resources, Strategic Plan, FY2012-2015

This report outlines the strategic plan for Iowa Department of Natural Resources, goals and mission.

Iowa Department of Management, Strategic Plan, 2012-2015

This report outlines the strategic plan for Department of Management, goals and mission.

Iowa National Guard Office of the Adjutant General Strategic Plan, 2008

This report outlines the strategic plan for Iowa National Guard Office of the Adjutant General, goals and mission.

Iowa Department of Human Rights Strategic Plan, 2012-2014

The Iowa Department for Human Rights Strategic Plan.

Iowa Communications Network, Strategic Plan, 2012

This report outlines the strategic plan for Iowa Communications Network, goals and mission.