Application of MLP and Kohonen networks for recognition of wear patterns of single-point dressers

Grinding is a parts finishing process for advanced products and surfaces. However, continuous friction between the workpiece and the grinding wheel causes the latter to lose its sharpness, thus impairing the grinding results. This is when the dressing process is required, which consists of sharpening the worn grains of the grinding wheel. The dressing conditions strongly affect the performance of the grinding operation; hence, monitoring them throughout the process can increase its efficiency. The objective of this study was to estimate the wear of a single-point dresser using intelligent systems whose inputs were obtained by the digital processing of acoustic emission signals. Two intelligent systems, the multilayer perceptron and the Kohonen neural network, were compared in terms of their classifying ability. The harmonic content of the acoustic emission signal was found to be influenced by the condition of dresser, and when used to feed the neural networks it is possible to classify the condition of the tool under study.

The Mode of Action of Recombinant Mycobacterium tuberculosis Shikimate Kinase: Kinetics and Thermodynamics Analyses

Tuberculosis remains as one of the main cause of mortality worldwide due to a single infectious agent, Mycobacterium tuberculosis. The aroK-encoded M. tuberculosis Shikimate Kinase (MtSK), shown to be essential for survival of bacilli, catalyzes the phosphoryl transfer from ATP to the carbon-3 hydroxyl group of shikimate (SKH), yielding shikimate-3-phosphate and ADP. Here we present purification to homogeneity, and oligomeric state determination of recombinant MtSK. Biochemical and biophysical data suggest that the chemical reaction catalyzed by monomeric MtSK follows a rapid-equilibrium random order of substrate binding, and ordered product release. Isothermal titration calorimetry (ITC) for binding of ligands to MtSK provided thermodynamic signatures of non-covalent interactions to each process. A comparison of steady-state kinetics parameters and equilibrium dissociation constant value determined by ITC showed that ATP binding does not increase the affinity of MtSK for SKH. We suggest that MtSK would more appropriately be described as an aroL-encoded type II shikimate kinase. Our manuscript also gives thermodynamic description of SKH binding to MtSK and data for the number of protons exchanged during this bimolecular interaction. The negative value for the change in constant pressure heat capacity (ΔCp) and molecular homology model building suggest a pronounced contribution of desolvation of non-polar groups upon binary complex formation. Thermodynamic parameters were deconvoluted into hydrophobic and vibrational contributions upon MtSK:SKH binary complex formation. Data for the number of protons exchanged during this bimolecular interaction are interpreted in light of a structural model to try to propose the likely amino acid side chains that are the proton donors to bulk solvent following MtSK:SKH complex formation. © 2013 Rosado et al.

On the influence of the mode-shapes of a marine propulsion shafting system on the prediction of torsional stresses

Torsional vibration predictions and measurements of a marine propulsion system, which has both damping and a highly flexible coupling, are presented in this paper. Using the conventional approach to stress prediction in the shafting system, the numerical predictions and the experimental torsional vibration stress curves in some parts of the shafting system are found to be quite different. The free torsional vibration characteristics and forced torsional vibration response of the system are analyzed in detail to investigate this phenomenon. It is found that the second to fourth natural modes of the shafting system have significant local deformation. This results in large torsional resonant responses in different sections of the system corresponding to different engine speeds. The results show that when there is significant local deformation in the shafting system for different modes, then multi-point measurements should be made, rather than the conventional method of using a single measurement at the free end of the shaft, to obtain the full torsional vibration characteristics of the shafting system.

Neural networks models for wear patterns recognition of single-point dresser

Grinding is a workpiece finishing process for advanced products and surfaces. However, the constant friction between workpiece and grinding wheel causes the latter to lose its sharpness, thereby impairing the result of the grinding process. When this occurs, the dressing process is essential to sharpen the worn grains of the grinding wheel. The dressing conditions strongly influence the performance of the grinding operation; hence, monitoring them throughout the process can increase its efficiency. The purpose of this study was to classify the wear condition of a single-point dresser using intelligent systems whose inputs were obtained by digitally processing acoustic emission signals. Two multilayer perceptron (MLP) neural networks were compared for their classification ability, one using the root mean square (RMS) statistics and another the ratio of power (ROP) statistics as input. In this study, it was found that the harmonic content of the acoustic emission signal is influenced by the condition of the dresser, and that the condition of the tool under study can be classified by using the aforementioned statistics to feed a neural network. © IFAC.

Assembling a xylanase-lichenase chimera through all-atom molecular dynamics simulations

Multifunctional enzyme engineering can improve enzyme cocktails for emerging biofuel technology. Molecular dynamics through structure-based models (SB) is an effective tool for assessing the tridimensional arrangement of chimeric enzymes as well as for inferring the functional practicability before experimental validation. This study describes the computational design of a bifunctional xylanase-lichenase chimera (XylLich) using the xynA and bglS genes from Bacillus subtilis. In silico analysis of the average solvent accessible surface area (SAS) and the root mean square fluctuation (RMSF) predicted a fully functional chimera, with minor fluctuations and variations along the polypeptide chains. Afterwards, the chimeric enzyme was built by fusing the xynA and bglS genes. XylLich was evaluated through small-angle X-ray scattering (SAXS) experiments, resulting in scattering curves with a very accurate fit to the theoretical protein model. The chimera preserved the biochemical characteristics of the parental enzymes, with the exception of a slight variation in the temperature of operation and the catalytic efficiency (k cat/Km). The absence of substantial shifts in the catalytic mode of operation was also verified. Furthermore, the production of chimeric enzymes could be more profitable than producing a single enzyme separately, based on comparing the recombinant protein production yield and the hydrolytic activity achieved for XylLich with that of the parental enzymes. © 2013 Elsevier B.V. All rights reserved.

Uma nova metodologia de projeto e controle para o inversor Boost (CSI) monofásico, para o aproveitamento de fontes alternativas e renováveis de energia elétrica

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Célula de comutação de três estados aplicada ao pré-regulador boost de estágio único e elevado fator de potência

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Pré-regulador retificador boost com controle digital por valores médios, para sistema de iluminação fluorescente multi-lâmpadas, utilizando dispositivo FPGA e VHDL

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Progress made in the implementation of the CARICOM single market and economy

Includes bibliography

Retificador entrelaçado boost, no modo de condução descontínua, com técnica de correção da corrente de entrada e elevado fator de potência, para aplicação em sistema trólebus

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Single-pass measurement of the optical quality of the opossum eye

This paper reports the results that are part of a series of experiments designed to evaluate aspects of the spatial resolution of the visual system of the opossum, Didelphis marsupialis aurita. This nocturnal marsupial presents a well-developed eye, displaying features that reflect specialization for operation at low levels of luminosity. The species was shown to be slightly myopic, a feature that may prove to be valuable because of the increased depth of field. Opossum visual acuity has been previously evaluated by means of determining the Contrast Sensitivity Function (CSF). The results indicate rather poor visual acuity compared with other nocturnal animals. In this paper, we describe the results obtained for the optical quality of the opossum's eye using a single-pass method. The results suggest that the opossum's optical system is capable of forming images that can be resolved when separated by an angular distance on the order of 6 minutes of arc.

Riboflavin analgs from Streptomyces davawensisas antiinfectives: mode of action, mechanism of resistance of the producer and metabolization by humans

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estudos teóricos e experimentais da operação conjunta de um supressor eletromagnético e de um filtro ativo de potência

Pós-graduação em Engenharia Elétrica - FEIS

Load Characterization and Revenue Metering Under Non-Sinusoidal and Asymmetrical Operation

This paper proposes an approach to load characterization and revenue metering, which accounts for the influence of supply deterioration and line impedance. It makes use of the Conservative Power Theory and aims at characterizing the load from the measurements done at the point of common coupling. Despite the inherent limitations of a single-point measurement, the proposed methodology enables evaluation of power terms, which clarify the effects of reactivity, asymmetry and distortion, and attempts to depurate the power consumption accounted to the load from those terms deriving from supply nonidealities.

Influence of abutment-to-fixture design on reliability and failure mode of all-ceramic crown systems

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)