Tractatus de in litem iurando, siue aurea, et solennis Repetitio L. Si quando C. vnde vi /

Mode of access: Internet.

Sparking : a comedietta, in one act and one scene : founded on L'Enticelle of Edouard Paileron /

Mode of access: Internet.

Allgemeines bu̇cher-lexikon, oder Vollstȧndiges alphabetisches verzeichniss der von 1700 bis zu ende ... [1892] erschienenen bu̇cher, welche in Deutschland und in den durch sprache und literatur damit verwandten lȧndern gedruckt worden sind. Nebst angabe der druckorte, der verleger und der preise.

Vols. 8-19 have added t.-p.: Allgemeines deutsches bücher-lexikon, oder Vollstȧndiges alphabetisches verzeichniss derjenigen schriften, welche in Deutschland und in den angrenzenden, mit der deutschen sprache und lite-

A manual of the principal instruments used in American engineering and surveying : manufactured by W. & L. E. Gurley, Troy, N.Y.

Cover title: Gurley's manual: American engineers' and surveyors' instruments.

A manual of the principal instruments used in American engineering and surveying, manufactured by W. & L.E. Gurley, Troy, N.Y.

Mode of access: Internet.

Final opinion on rehearing, filed on April 20, 1927 by the Supreme Court of Illinois in the O'Gara Coal Company vs. Louis L. Emerson, secretary of state.

Published by the Corporation Trust Company, Chicago.

Objections to Calvinism as it is : in a series of letters addressed to N. L. Rice /

Mode of access: Internet.

Shift Scheduling in Services When Employees Have Limited Availability: An L. P. Approach

This paper compares two linear programming (LP) models for shift scheduling in services where homogeneously-skilled employees are available at limited times. Although both models are based on set covering approaches, one explicitly matches employees to shifts, while the other imposes this matching implicitly. Each model is used in three forms—one with complete, another with very limited meal break placement flexibility, and a third without meal breaks—to provide initial schedules to a completion/improvement heuristic. The term completion/improvement heuristic is used to describe a construction/ improvement heuristic operating on a starting schedule. On 80 test problems varying widely in scheduling flexibility, employee staffing requirements, and employee availability characteristics, all six LP-based procedures generated lower cost schedules than a comparison from-scratch construction/improvement heuristic. This heuristic, which perpetually maintains an explicit matching of employees to shifts, consists of three phases which add, drop, and modify shifts. In terms of schedule cost, schedule generation time, and model size, the procedures based on the implicit model performed better, as a group, than those based on the explicit model. The LP model with complete break placement flexibility and implicitly matching employees to shifts generated schedules costing 6.7% less than those developed by the from-scratch heuristic.

La gestione del portafoglio brevettuale del Dipartimento Energia e Trasporti

Prima della presente riforma del CNR, esisteva all’interno dell’Ente un Ufficio Brevetti, che supportava i propri ricercatori, a livello nazionale, nelle fasi di primo deposito, estensione all’estero, nella stipula di contratti di cessione e di licenza. Dal momento in cui è entrato in vigore il decreto di riordino dell’Ente (D.L. 4 giugno 2003, n. 127) e si sono creati e avviati gli 11 Dipartimenti, l’ufficio brevetti è stato soppresso e il portafoglio brevettuale è stato affidato ai Dipartimenti, ognuno con la propria macro area di competenza.

Proteomic and phosphoproteomic analysis of polyethylene glycol-induced osmotic stress in root tips of common bean (Phaseolus vulgaris L.)

Previous studies have shown that polyethylene glycol (PEG)-induced osmotic stress (OS) reduces cell-wall (CW) porosity and limits aluminium (Al) uptake by root tips of common bean (Phaseolus vulgaris L.). A subsequent transcriptomic study suggested that genes related to CW processes are involved in adjustment to OS. In this study, a proteomic and phosphoproteomic approach was applied to identify OS-induced protein regulation to further improve our understanding of how OS affects Al accumulation. Analysis of total soluble proteins in root tips indicated that, in total, 22 proteins were differentially regulated by OS; these proteins were functionally categorized. Seventy-seven per- cent of the total expressed proteins were involved in metabolic pathways, particularly of carbohydrate and amino acid metabolism. An analysis of the apoplastic proteome revealed that OS reduced the level of five proteins and increased that of seven proteins. Investigation of the total soluble phosphoproteome suggested that dehydrin responded to OS with an enhanced phosphorylation state without a change in abundance. A cellular immunolocalization analysis indicated that dehydrin was localized mainly in the CW. This suggests that dehydrin may play a major protective role in the OS-induced physical breakdown of the CW structure and thus maintenance of the reversibility of CW extensibility during recovery from OS. The proteomic and phosphoproteomic analyses provided novel insights into the complex mechanisms of OS-induced reduction of Al accumulation in the root tips of common bean and highlight a key role for modification of CW structure.

Construção de modelos de interação in silico e in vitro do inibidor do tipo Kunitz de Adenanthera pavonina L. para as enzimas cisteínicas e serínicas

Serines proteinases inhibitors (PIs) are widely distributed in nature and are able to inhibit both in vitro and in vivo enzymatic activites. Seed PIs in than leguminous are classified in seven families, Bowman-Birk and Kunitz type families that most studied representing an important role in the first line of defense toward insects pests. Some Kunitz type inhibitors possess activities serine and cysteine for proteinases named bifunctional inhibitor, as ApTKI the inhibitor isolate from seed of Adenanthera pavonina. The A. pavonina inhibitor presenting the uncommon property and was used for interaction studies between proteinases serine (trypsin) and cysteine (papain). In order to determinate the in vitro interaction of ApTKI against enzymes inhibitor purification was carried cut by using chromatographic techniques and inhibition assays. The 3D model of the bifunctional inhibitor ApTKI was constructed SWISS-MODEL program by homology modeling using soybean trypsin inhibitor (STI, pdb:1ba7), as template which presented 40% of identity to A. pavonina inhibitor. Model quality was evaluated by PROCHECK program. Moreover in silico analyzes of formed complex between the enzymes and ApTKI was evaluated by HEX 4.5 program. In vitro results confirmed the inhibitory assays, where the inhibitor presented the ability to simultaneously inhibit trypsin and papain. The residues encountered in the inhibitor model of folder structural three-dimensional that make contact to enzymes target coud explain the specificity pattern against serine and cysteine proteinases