Operator's manual : water purification unit, reverse osmosis, 600 GPH trailer mounted ... model 0996108001 (4610-01-234-2190).

Includes index.

A practical treatise on railway curves and location : for young engineers : containing a full description of the instruments, the manner of adjusting them, and the methods of proceeding in the field, new and simple formulae for compound and reverse curving, rules for calculating excavation and embankment, staking out work, &c., together with tables of natural sines and tangents, radii, chords, ordinates, and others of general use in the profession /

On spine: Shunk on railway curves &c.

Organizational, direct support and general support maintenance repair parts and special tool list for water purification unit, reverse osmosis, 600 GPH trailer mounted, flatbed cargo, 5 ton 4 wheel tandem model Rowpu 600-1, (4610-01-093-2380).

"September 1983."

Operator, organizational, direct and general support, and depot maintenance manual (including repair parts) : welding machine, arc, inert shielded, transformer, 300 amp, 10 to 400 amp AC straight polarity, 10 to 00 amp DC reverse polarity (Midstates model MAG 00 AC/DC T 14) FSN 3431-862-6670.

"February 1967."

Organizational, direct support, and general support maintenance manual : water purification unit, reverse osmosis, 600 GPH trailer mounted, flatbed cargo, 5 ton 4 wheel tandem model Rowpu 600-1 (4610-01-093-2380) and 600 GPH skid mounted model Rowpu 600-3 (4610-01-113-8651).

Includes index.

Engine crew with log train, one man raising hat (names inscribed on reverse)

from left: 1 - Wm. Tate, conductor, on pilot [others unidentified]

Second Lieutenant William B. McCreery, July 21, 1861 (date of battle), On reverse: First Battle of Bull Run on the battlefield, Description: three soldiers under tree with swords drawn

Scene showing a group of men, women, and children. The men appear to be members of the Grand Army of the Republic, possibly the Gov. Crapo Post #145

California digest supplement, 1915-1919; a digest of the cases decided by the Supreme and Appellate courts of California and by the federal courts on questions of California law since Kerr's California digest, as contained in Raymond's 1916 supplement and in the 1917-1919 California current digests, inclusive, up to volume 178 California Supreme court reports, volume 37 California Appellate reports, and up to 58 California decisions, page 137; California Appellate decisions, page 258; 182 Pacific reporter (California cases), page 991; 258 Federal reporter (California cases), page 161; 39 Supreme court reporter (California cases), page 568. With references to Ruling case law, and important notes in American decisions, American state reports, Annotated cases, Lawyers' reports annotated, and American law reports, also an alphabetical table, direct and reverse, of all cases digested, showing on what page and under what subject each case is found digested in this digest, and also where the various California cases are cited in annotations in "The American series of annotated reports".

Mode of access: Internet.

A sensitive, specific, and cost-effective multiplex reverse transcriptase-PCR assay for the detection of seven common respiratory viruses in respiratory samples

Cell culture and direct fluorescent antibody (DFA) assays have been traditionally used for the laboratory diagnosis of respiratory viral infections. Multiplex reverse transcriptase polymerase chain reaction (m-RT-PCR) is a sensitive, specific, and rapid method for detecting several DNIA and RNA viruses in a single specimen. We developed a m-RT-PCR assay that utilizes multiple virus-specific primer pairs in a single reaction mix combined with an enzyme-linked amplicon hybridization assay (ELAHA) using virus-specific probes targeting unique gene sequences for each virus. Using this m-RT-PCR-ELAHA, we examined the presence of seven respiratory viruses in 598 nasopharyngeal aspirate (NPA) samples from patients with suspected respiratory infection. The specificity of each assay was 100%. The sensitivity of the DFA was 79.7% and the combined DFA/culture amplified-DFA (CA-DFA) was 88.6% when compared to the m-RT-PCR-ELAHA. Of the 598 NPA specimens screened by m-RT-PCR-ELAHA, 3% were positive for adenovirus (ADM), 2% for influenza A (Flu A) virus, 0.3% for influenza B (Flu B) virus, 1% for parainfluenza type I virus (PIV1), 1% for parainfluenza type 2 virus (PIV2), 5.5% for parainfluenza type 3 virus (PIV3), and 21% for respiratory syncytial virus (RSV). The enhanced sensitivity, specificity, rapid result turnaround time and reduced expense of the m-RT-PCR-ELAHA compared to DFA and CA-DFA, suggests that this assay would be a significant improvement over traditional assays for the detection of respiratory viruses in a clinical laboratory.

The inhibitory effect of pentobarbitone on reverse transcription-PCR

Pentobarbitone sodium (Sodium 5-ethyl-5[1-methylbutyl]-pentobarbitone) is a short-acting barbiturate that is commonly used to euthanase animals. As part of our studies into the molecular genetics of copper toxicosis in Bedlington terrier dogs, reverse-transcription (RT)-PCR was noted to always fail on RNA samples collected from livers of dogs sacrificed by pentobarbitone injection. When samples were collected without pentobarbitone, however, RTPCR was always successful. We suspected the possible inhibition by pentobarbitone sodium of either reverse transcriptase or Taq polymerase. In vitro studies showed that pentobarbitone interference of PCR occurred at >4 mug/mul. To identify if pentobarbitone produced competitive inhibition, each components (Taq polymerase, MgCl2, dNTP, etc.) of the PCR was individually altered. However, inhibition still persisted, suggesting that multiple PCR components may be affected. Also it was shown that pentobarbitone interference was not dependent on the PCR product size. Simple dilution of pentobarbitone contaminated DNA solutions, and the addition of bovine serum albumin (BSA) to the PCR mix overcame pentobarbitone interference. In vivo, PCR by pentobarbitone was found to be compounded by high DNA concentration and pentobarbitone contamination. In addition, both high DNA concentration and pentobarbitone contamination could be overcome through dilution and the addition of BSA. Further work is required to quantify pentobarbitone concentration in the liver-extracted DNA and RNA samples before this inhibition effect on PCR can be fully elucidated. (C) 2004 Elsevier B.V. All rights reserved.

Real-time reverse transcriptase PCR for the endogenous koala retrovirus reveals an association between plasma viral load and neoplastic disease in koalas

Koala retrovirus (KoRV) is a newly described endogenous retrovirus and is unusual in that inserts comprise a full-length replication competent genome. As koalas are known to suffer from an extremely high incidence of leukaemia/lymphoma, the association between this retrovirus and disease in koalas was examined. Using quantitative real-time reverse transcriptase PCR it was demonstrated that KoRV RNA levels in plasma are significantly increased in animals suffering from leukaemia or lymphoma when compared with healthy animals. Increased levels of KoRV were also seen for animals with clinical chlamydiosis. A significant positive association between viral RNA levels and age was also demonstrated. Real-time PCR demonstrated as much as 5 log variation in KoRV proviral DNA levels in genomic DNA extracted from whole blood from different animals. Taken together these data indicate that KoRV is an active endogenous retrovirus and suggests that it may be causally linked to neoplastic disease in koalas.