Philopatry of male marine turtles inferred from mitochondrial DNA markers

Recent studies of mitochondrial DNA (mtDNA) variation among marine turtle populations are consistent with the hypothesis that females return to beaches in their natal region to nest as adults. In contrast, less is known about breeding migrations of male marine turtles and whether they too are philopatric to natal regions. Studies of geographic structuring of restriction fragment and microsatellite polymorphisms at anonymous nuclear loci in green turtle (Chelonia mydas) populations indicate that nuclear gene flow is higher than estimates from mtDNA analyses. Regional populations from the northern and southern Great Barrier Reef were distinct for mtDNA but indistinguishable at nuclear loci, whereas the Gulf of Carpentaria (northern Australia) population was distinct for both types of marker. To assess whether this result was due to reduced philopatry of males across the Great Barrier Reef, we determined the mtDNA haplotypes of breeding males at courtship areas for comparison with breeding females from the same three locations. We used a PCR-restriction fragment length polymorphism approach to determine control region haplotypes and designed mismatch primers for the identification of specific haplotypes. The mtDNA haplotype frequencies were not significantly different between males and females at any of the three areas and estimates of Fst among the regions were similar for males and females (Fst = 0.78 and 0.73, respectively). We conclude that breeding males, like females, are philopatric to courtship areas within their natal region. Nuclear gene flow between populations is most likely occurring through matings during migrations of both males and females through nonnatal courtship areas.

Unblocking of chain-terminated primer by HIV-1 reverse transcriptase through a nucleotide-dependent mechanism

HIV-1 replication is inhibited by the incorporation of chain-terminating nucleotides at the 3′ end of the growing DNA chain. Here we show a nucleotide-dependent reaction catalyzed by HIV-1 reverse transcriptase that can efficiently remove the chain-terminating residue, yielding an extendible primer terminus. Radioactively labeled 3′-terminal residue from the primer can be transferred into a product that is resistant to calf intestinal alkaline phosphatase and sensitive to cleavage by snake venom phosphodiesterase. The products formed from different nucleotide substrates have unique electrophoretic migrations and have been identified as dinucleoside tri- or tetraphosphates. The reaction is inhibited by dNTPs that are complementary to the next position on the template (Ki ≈ 5 μM), suggesting competition between dinucleoside polyphosphate synthesis and DNA polymerization. Dinucleoside polyphosphate synthesis was inhibited by an HIV-1 specific non-nucleoside inhibitor and was absent in mutant HIV-1 reverse transcriptase deficient in polymerase activity, indicating that this activity requires a functional polymerase active site. We suggest that dinucleoside polyphosphate synthesis occurs by transfer of the 3′ nucleotide from the primer to the pyrophosphate moiety in the nucleoside di- or triphosphate substrate through a mechanism analogous to pyrophosphorolysis. Unlike pyrophosphorolysis, however, the reaction is nucleotide-dependent, is resistant to pyrophosphatase, and produces dinucleoside polyphosphates. Because it occurs at physiological concentrations of ribonucleoside triphosphates, this reaction may determine the in vivo activity of many nucleoside antiretroviral drugs.

The origin of the Na-Dene

Linguistic evidence indicates that the Yeniseian family of languages, spoken in central Siberia, is most closely related to the Na-Dene family of languages spoken, for the most part, in northwestern North America. This hypothesis locates the source of one of the three migrations responsible for the peopling of the Americas.

Asian genotypes of JC virus in Native Americans and in a Pacific Island population: Markers of viral evolution and human migration

The human polyomavirus JC (JCV) causes the central nervous system demyelinating disease progressive multifocal leukoencephalopathy. Previously, we showed that 40% of Caucasians in the United States excrete JCV in the urine as detected by PCR. We have now studied 68 Navaho from New Mexico, 25 Flathead from Montana, and 29 Chamorro from Guam. By using PCR amplification of a fragment of the VP1 gene, JCV DNA was detected in the urine of 45 (66%) Navaho, 14 (56%) Flathead, and 20 (69%) Chamorro. Genotyping of viral DNAs in these cohorts by cycle sequencing showed predominantly type 2 (Asian), rather than type 1 (European). Type 1 is the major type in the United States and Hungary. Type 2 can be further subdivided into 2A, 2B, and 2C. Type 2A is found in China and Japan. Type 2B is a subtype related to the East Asian type, and is now found in Europe and the United States. The large majority (56–89%) of strains excreted by Native Americans and Pacific Islanders were the type 2A subtype, consistent with the origin of these strains in Asia. These findings indicate that JCV infection of Native Americans predates contact with Europeans, and likely predates migration of Amerind ancestors across the Bering land bridge around 12,000–30,000 years ago. If JCV had already differentiated into stable modern genotypes and subtypes prior to first settlement, the origin of JCV in humans may date from 50,000 to 100,000 years ago or more. We conclude that JCV may have coevolved with the human species, and that it provides a convenient marker for human migrations in both prehistoric and modern times.

Climate change is affecting altitudinal migrants and hibernating species

Calendar date of the beginning of the growing season at high altitude in the Colorado Rocky Mountains is variable but has not changed significantly over the past 25 years. This result differs from growing evidence from low altitudes that climate change is resulting in a longer growing season, earlier migrations, and earlier reproduction in a variety of taxa. At our study site, the beginning of the growing season is controlled by melting of the previous winter's snowpack. Despite a trend for warmer spring temperatures the average date of snowmelt has not changed, perhaps because of the trend for increased winter precipitation. This disjunction between phenology at low and high altitudes may create problems for species, such as many birds, that migrate over altitudinal gradients. We present data indicating that this already may be true for American robins, which are arriving 14 days earlier than they did in 1981; the interval between arrival date and the first date of bare ground has grown by 18 days. We also report evidence for an effect of climate change on hibernation behavior; yellow-bellied marmots are emerging 38 days earlier than 23 years ago, apparently in response to warmer spring air temperatures. Migrants and hibernators may experience problems as a consequence of these changes in phenology, which may be exacerbated if climate models are correct in their predictions of increased winter snowfall in our study area. The trends we report for earlier formation of permanent snowpack and for a longer period of snow cover also have implications for hibernating species.

Polynesian origins: Insights from the Y chromosome

The question surrounding the colonization of Polynesia has remained controversial. Two hypotheses, one postulating Taiwan as the putative homeland and the other asserting a Melanesian origin of the Polynesian people, have received considerable attention. In this work, we present haplotype data based on the distribution of 19 biallelic polymorphisms on the Y chromosome in a sample of 551 male individuals from 36 populations living in Southeast Asia, Taiwan, Micronesia, Melanesia, and Polynesia. Surprisingly, nearly none of the Taiwanese Y haplotypes were found in Micronesia and Polynesia. Likewise, a Melanesian-specific haplotype was not found among the Polynesians. However, all of the Polynesian, Micronesian, and Taiwanese haplotypes are present in the extant Southeast Asian populations. Evidently, the Y-chromosome data do not lend support to either of the prevailing hypotheses. Rather, we postulate that Southeast Asia provided a genetic source for two independent migrations, one toward Taiwan and the other toward Polynesia through island Southeast Asia.

Multiple maternal origins and weak phylogeographic structure in domestic goats

Domestic animals have played a key role in human history. Despite their importance, however, the origins of most domestic species remain poorly understood. We assessed the phylogenetic history and population structure of domestic goats by sequencing a hypervariable segment (481 bp) of the mtDNA control region from 406 goats representing 88 breeds distributed across the Old World. Phylogeographic analysis revealed three highly divergent goat lineages (estimated divergence >200,000 years ago), with one lineage occurring only in eastern and southern Asia. A remarkably similar pattern exists in cattle, sheep, and pigs. These results, combined with recent archaeological findings, suggest that goats and other farm animals have multiple maternal origins with a possible center of origin in Asia, as well as in the Fertile Crescent. The pattern of goat mtDNA diversity suggests that all three lineages have undergone population expansions, but that the expansion was relatively recent for two of the lineages (including the Asian lineage). Goat populations are surprisingly less genetically structured than cattle populations. In goats only ≈10% of the mtDNA variation is partitioned among continents. In cattle the amount is ≥50%. This weak structuring suggests extensive intercontinental transportation of goats and has intriguing implications about the importance of goats in historical human migrations and commerce.

Orquestração de migração massiva de máquinas virtuais baseada em análise cíclica para ambientes de computação na nuvem.

Um das principais características da tecnologia de virtualização é a Live Migration, que permite que máquinas virtuais sejam movimentadas entre máquinas físicas sem a interrupção da execução. Esta característica habilita a implementação de políticas mais sofisticadas dentro de um ambiente de computação na nuvem, como a otimização de uso de energia elétrica e recursos computacionais. Entretanto, a Live Migration pode impor severa degradação de desempenho nas aplicações das máquinas virtuais e causar diversos impactos na infraestrutura dos provedores de serviço, como congestionamento de rede e máquinas virtuais co-existentes nas máquinas físicas. Diferente de diversos estudos, este estudo considera a carga de trabalho da máquina virtual um importante fator e argumenta que escolhendo o momento adequado para a migração da máquina virtual pode-se reduzir as penalidades impostas pela Live Migration. Este trabalho introduz a Application-aware Live Migration (ALMA), que intercepta as submissões de Live Migration e, baseado na carga de trabalho da aplicação, adia a migração para um momento mais favorável. Os experimentos conduzidos neste trabalho mostraram que a arquitetura reduziu em até 74% o tempo das migrações para os experimentos com benchmarks e em até 67% os experimentos com carga de trabalho real. A transferência de dados causada pela Live Migration foi reduzida em até 62%. Além disso, o presente introduz um modelo que faz a predição do custo da Live Migration para a carga de trabalho e também um algoritmo de migração que não é sensível à utilização de memória da máquina virtual.

Más allá del turismo: movilidad residencial europea y nuevos núcleos urbanos

Desde 1960 se han intensificado los flujos de turismo residencial internacional y las migraciones intra-europeas de jubilados hacia el Mediterráneo. La plasmación territorial de este proceso ha generado nuevas estructuras duales. Se analiza la situación de las comunidades noreuropeas que residen en urbanizaciones separadas de los núcleos urbanos tradicionales, con atención a la provincia de Alicante.

Migraciones interregionales en España (1960-1989): cambios y barreras

En este trabajo se analizan los cambios en el patrón de las migraciones interiores españolas a lo largo del período 1960-1989. De forma preliminar y de acuerdo con las teorías explicativas de los flujos migratorios, se contrasta econométricamente la consistencia de estos cambios con la existencia de sistemas regionales económicamente desequilibrados versus sistemas regionales con diferencias compensadoras. Como resultado, se encuentra que a pesar de la reciente intensificación de las migraciones interiores y a diferencia de lo sucedido en otras etapas y bajo otras circunstancias, ahora no se debería confiar en que los movimientos migratorios puedan contribuir al acercamiento a la media nacional de las regiones con tasas de desempleo más elevadas. Esta vía podría haberse agotado en la medida en que el peso de las barreras procedentes de los mercados de trabajo y de los mercados inmobiliarios es creciente y, paradójicamente, ha generado flujos inte-rregionales equilibrados y no polarizados. Si, además, la posibilidad de que la emigración cumpla un papel dinamizador del ajuste económico se ve afectada por el componente de desempleo de los trabajadores que se desplazan, entonces las políticas públicas no deberían centrarse en la incentivación de los flujos de forma indiscriminada sino, más bien, en los aspectos microeconómicos referentes a la disponibilidad de información, la orientación y la capacitación de los potenciales trabajadores emigrantes.

Migrantes y migraciones: de nuevo la divergencia en las fuentes estadísticas

Este trabajo plantea una serie de deficiencias presentes en la Encuesta de Migraciones (EM), publicada anualmente por el INE a partir de la EPA. Consciente de la subestimación de la movilidad procedente de esta fuente estadística, el INE ha introducido recientemente ciertos cambios metodológicos. Sin embargo, no se han obtenido mejoras significativas y la misma continúa disponible para los investigadores. Las razones que se encuentran detrás de la limitada calidad de la EM son de tipo estadístico y afectan a la precisión y al sesgo de las estimaciones. Se desatienden principios básicos de la estimación para dominios pequeños, se incumplen los supuestos del muestreo por conglomerados y se incurre en graves sesgos debidos, probablemente, a la falta de respuesta. Consideramos que la investigación del hecho migratorio a través de la técnica del muestreo estratificado de poblaciones exige un tratamiento específico en la encuesta, dado el tamaño y las características de este dominio.

El nuevo mapa de las migraciones interiores en España: los cambios en el patrón de los sesenta

En el trabajo se aborda el estudio del espectacular crecimiento de la movilidad interior en la década de los noventa en España describiendo los principales rasgos de los flujos y las tasas migratorias. Además, se establecen las diferencias entre el actual patrón migratorio y el de los años sesenta. A partir del conjunto de las variables migratorias provinciales y utilizando la técnica de análisis cluster se establece una tipología migratoria de las provincias españolas. Con dicha clasificación se comprueba, además, la existencia de una clara correspondencia entre la conducta migratoria y la dinámica de las principales variables socioeconómicas provinciales.

Reemigrar en España: una aproximación a sus determinantes

En este trabajo se investiga sobre las razones que inducen a los migrantes a realizar migraciones múltiples. Tras el análisis descriptivo de las características de estos inmigrantes, se especifica un logit binomial y un probit multinomial en los que la probabilidad de volver a emigrar depende de las características personales y de las cadenas migratorias de orígenes y destinos. La información utilizada procede de los microdatos de la Estadística de Variaciones Residenciales. Los resultados indican que las migraciones repetidas de españoles y extranjeros responden a motivos diferentes. El análisis sugiere que las reemigraciones de los extranjeros obedecerían a los resultados de sus experiencias laborales, mientras que en las de los españoles parecen hallarse otros motivos adicionales.

Migraciones: ¿qué describen el Censo de 2001 y la Estadística de Variaciones Residenciales?

Comunicación presentada en el IX Encuentro de Economía Aplicada, Jaén, 8-10 junio 2006.

¿Son fiables los datos de migraciones del censo de 2001?

El trabajo se centra en explicar las diferencias mostradas por la Estadística de Variaciones Residenciales (EVR) y el Censo de Población de 2001 en la captación de la movilidad de la población española entre 1991 y 2001. Encontramos que las migraciones recogidas en el Censo se han alejado espectacularmente de las declaradas en las variaciones residenciales. Tras analizar en profundidad ambas fuentes estadísticas, pensamos que el Censo de 2001 presenta para esta variable importantes problemas de coherencia y de falta de respuesta, por lo que no resulta una fuente estadística fiable con la que contrastar los actuales niveles de movilidad de la población.