Effect of different feeding regimes on reproduction and survival of ablated Penaeus monodon Fabricius

Results of experiments conducted on ablated Penaeus monodon, fed with 4 different feeds (pellet-pellet, mussel-mussel, mussel-pellet and squid-pellet), on survival, spawning, fecundity and hatching rate are given and discussed.

Aniracetam restores the effects of amyloid-beta protein or ageing on membrane fluidity and intracellular calcium concentration in mice synaptosomes

In the present study, we observed the in vitro effect of aniracetam on membrane fluidity and free calcium concentrations (Ca(2+)i) of frontal cortical (FC) and hippocampal (HP) synaptosomes of aged mice and young mice treated with amyloid-beta protein (A

The effect of various salinity levels and stocking density manipulation methods on the survival of milkfish fry (Chanos chanos Forsskal) during storage

Results of the study indicate that the survival rate and increase in body weight did not differ significantly at different salinity levels or at different stocking density manipulation methods. A significant interaction between salinity and stocking density manipulation could not be demonstrated statistically. There apparently is no need to reduce the salinity of the water used in storing milkfish Chanos chanos fry in order to attain higher survival as commonly believed. Sufficient food and maintenance of good water quality are more important than salinity for higher survival of fry during storage.

Relationship between trichosanthin cytotoxicity and its intracellular concentration

Trichosanthin (TCS) is a type I ribosome-inactivating protein with board spectrum of biological activity. Toxicity of this compound differs in different cell lines and this study examined the cause of such difference. It is generally believed that TCS toxicity is mediated through intracellular ribosome inactivation. Therefore, TCS toxicity should be determined by the amount inside cells rather than outside. Three different cell types IC21, JAR and Vero cell lines were chosen with high, medium and low sensitivity to TCS. Intracellular concentrations of fluorescein isothiocyanate labeled TCS were determined by laser scanning confocal microscopy. A good relationship was demonstrated between intracellular TCS concentration and toxicity. Highest intracellular concentration was found in IC21, followed by JAR, and lowest in Vero cells. When the intracellular TCS concentrations in these cells were reduced by using a competitive inhibitor to block cell entry, cytotoxicity was not observed. In conclusion, there is strong evidence to indicate that cytotoxicity of TCS is dependent on its intracellular concentration. Variation of cytotoxicity in different cells may be related to the mechanisms affecting its internalization. (C) 2002 Published by Elsevier Science Ireland Ltd.

Growth and survival of Penaeus monodon Fabricius zoeae on different diatom feeds

A study was undertaken to determine the most suitable food for Penaeus monodon zoea out of mixed diatoms, Skeletonema costatum, Chaetoceros calcitrans and C. gracilis. Results indicate that mixed diatoms and C. gracilis are the better food. Addition of zooplankton, e.g.rotifers, is also recommended as early as Z3 since the larvae start to be omnivorous at this stage.

Effects of water movement and aeration system on the survival and growth of hatchery bred sugpo (Penaeus monodon Fabricius) in earthen nursery ponds

An experiment was conducted to determine the effects of water movement and airlift aeration on the survival and growth of P. monodon fry reared from P sub(4)P sub(5) to P sub(32)P sub(33) in earthen brackishwater ponds. The high survival rates obtained justify the need for aeration when using the earliest stages of fry (P sub(4)P sub(5)) at higher stocking densities. For older stages regardless of source and at lower stocking densities, nursery operations based on traditional methods could also achieve better survival rates.

Growth and survival rates of hatchery-produced and wild milkfish fry grown to fingerling size in earthen nursery ponds

Growth and survival rates of hatchery-produced and wild milkfish (Chanos chanos ) fry grown to fingerling size were compared. Data show no significant difference between the 2 fry. At a recommended stocking density of 30 fry/m super(2), hatchery-produced milkfish fry could attain fingerling size of almost 2g with a survival of 68%. The study indicates that hatchery-produced fry/fingerlings can equal the culture performance of the wild fry. Comparative performance of hatchery-bred and wild fry should encourage intensified research on milkfish broodstock development and refinement of induced spawning methods.

Survival, growth and production of Penaeus monodon at different stocking densities in earthen ponds with flow-through system and supplementary feeding

The survival, growth and production of Penaeus monodon in earthen ponds were observed. A significant effect of stocking density on the survival rate of prawns was observed. The final average weight obtained varied inversly with the stocking density and the production varied directly with stocking density and survival rates. The maintenance of a clear water environment is recommended as a preventive measure against Vorticella attack.

Survival, growth and production of Penaeus monodon and P. indicus at different density combinations with milkfish

Penaeus monodon and P. indicus juveniles were stocked and reared for about 3 months in earthen ponds at different density combinations with Chanos chanos. The presence of either Penaeus species at any density ratio did not affect significantly the C. chanos survival. Survival rates of the penaeids indicated that intraspecific and interspecific competition occurred and were reduced with the reduction in stocking rate. It is concluded that further studies on higher density ratios and feeding and economic consideration would be of help to the development of this kind of fishpond management system.

Effects of temperature on behavior, growth, development and survival of young milkfish, Chanos chanos Forsskal

The effects of 3 temperature treatments on activity, feeding, growth, development and survival of young milkfish (Chanos chanos) were investigated under laboratory conditions. It is believed that the results may be applied to develop a land-based mass production technology in rearing milkfish fry to fingerlings.

Survival and changes in the fine structure of selected tissues of Penaeus monodon Fabricius juveniles fed various carbohydrates

Penaeus monodon juveniles were reared on semipurified diets containing various carbohydrates (maltose, sucrose, dextrin, molasses, cassava starch, corn starch or sago palm starch). Significant differences were observed between the type as well as the level of carbohydrate in the diet on the survival of the juveniles. Results indicate that there does not seem to be any correlation between survival and the complexity of the carbohydrates.

The effect of various protein levels on the growth and survival rates of Penaeus monodon Fabricius

Postlarvae of tiger prawns, P. monodon, were fed with various protein levels of 0; 30; 40 and 50%, fish meal and rice bran were combined and pelletized for 16 wks. Prawns used had an average mean length of 1.2 mm and an average mean weight of 5.5 mg. Nitrite, ammonia, dissolved oxygen content, temperature and pH were monitored. After one month of feeding, growth increments of postlarvae fed with 30% protein diet were significantly different from those given 0; 40 and 50%. Survival rate of the group fed 40% protein was higher (81.7%) but was not significantly different from those fed 30% (68.0%). The highest feed efficiency was obtained from the 30% protein pellets (4.6). At the end of 16 weeks, growth increment of 40% protein level was significantly different from the 30% and 50% protein levels, but the survival rate was not significantly different from the 30% protein level. Feed conversion for the 40% protein diet was 2.8.

Survival, maturation, fecundity and hatching rates of unablated and ablated Penaeus indicus H.M. Edwards from brackishwater ponds

Penaeus indicus harvested after three months of rearing in brackishwater ponds and averaging 6.9 g for females and 5.6 g for males were stocked in two 12 cu m flowthrough ferrocement tanks at 240 females and 200 males per tank. The females were ablated on one eyestalk in one tank and remained unablated in the other tank; all males were unablated. Ablated females spawned up to 7 times per female; unablated females spawned up to only 3 times during the two month duration of the experiment. Ablated females produced a total number of 17.5 x 10⁶ eggs, 6.6 x 10⁶ nauplii, and an average of 23,480 eggs/spawning and 37.8% hatching rate from a total of 757 spawnings. Unablated females produced a total of 2.0 x 10⁶ eggs, 1.1 x 10⁶ nauplii, and an average of 26.990 eggs/spawning and 53.9% hatching rate from a total of 74 spawnings. Survival of ablated females was 53.5% compared to 69.4% for unablated females; males in both tanks averaged more than 90% survival.

Incidence and low temperature survival of Salmonella in fishery products

Salmonella was isolated from 12% of PD shrimps, 10% of HL shrimps, 14% of PUD shrimps, 17% of lobsters, 14% of cuttle fish, 25% of cat fish and 20% of seer fish (all frozen) tested. One percent of the fish meal, 4% of dried non-penaeid prawn and 23% of sea beach sand showed incidence of the organism. Salmonella was also isolated from 2 and 4% of the swab samples of utensil surfaces and the floor surface of the processing hall respectively as well as from 1% of the process water tested. All the serotypes of Salmonella tested were resistant to freezing at -40°C, but during subsequent storage at -20°C, there was some difference between the serotypes with regard to their viability, S. paratyphi B being the most resistant which survived up to 9 months while S. saintpaul the least resistant having survival up to 5 months only.