Gut filling, gut content and fatty acid composition of demersal fish species sampled during POLARSTERN cruise ANT-XXIV/2

The gut contents and fatty acid composition of 49 fish belonging to five Antarctic demersal families (Nototheniidae, Macrouridae, Channichtyidae, Bathydraconidae and Artedidraconidae) sampled at two stations at the Southern Ocean shelf and deep sea (600 and 2150 m) were analysed in order to identify their main food resource by linking trophic biomarkers with the dietary items found in the fish guts. Main food items of most fish analysed were amphipod crustaceans (e.g. in 63% of Trematomus bernachii guts) and polychaetes (e.g. in 80% of Bathydraco sp. guts), but other food items including fish, other crustaceans and gastropods were also ingested. The most prominent fatty acids found were 20:5(n-3), 16:0, 22:6(n-3) and 18:1(n-9). The results of gut content and fatty acid analyses indicate that all fish except the Channichthyidae share similar food resources irrespective of their depth distribution, i.e. benthic amphipods and polychaetes. A difference of the dietary spectrum can be observed with ontogenetic phases rather than between species, as high values of typical calanoid copepod marker fatty acids as 22:1(n-11) indicate that younger (smaller) specimens include more zooplankton in their diet.

Grain sizes, carbon content and clay minerals of sediments obtained from north Germany and the Baltic Sea

Clay mineralogy and geotechnical properties of Tarras clay, basin clays and tills from some parts of Schleswig-Holstein: Tarras clay of lower Eocene age, Quaternary till containing various admixtures of Tarras clay as well as basin clay and varve-clay from Schleswig-Holstein were investigated. Grain size distribution and soil mechanic characteristics were determined, which indicated different geotechnical properties for each sediment type.

(Table 1) Vertical distribution of relative contents of the main planktonic groups in the water column of the Kuril-Kamchatka region at Station VITYZ5635

Determinations were made of contents of carbon, lipids, nitrogen and, in some material, protein, carbohydrates, elementary composition of lipids and their spectral composition in total plankton samples from different depths (from the surface to 3000 m) and in several species of macroplanktonic deep-water crustaceans (decapods and mysids) living at different depths. Content of organic carbon and lipids in total plankton is high (40 to 60 and 35 to 70% of dry weight, respectively) and it does not change significantly with increasing depth. Deep-water macroplanktonic crustaceans have extremely high content of organic carbon and lipids, but there are no significant differences in this respect between species that live in different layers of the deep-water zone. Elementary composition of lipids indicates that they are highly saturated, with a marked predominance of unsaponifiable fraction, about 20% of which consists of methane hydrocarbons.

(Table 2) Fauna Harpacticoida and its distribution in the Kandalaksha Bay (White Sea) in July 1998 and 1999

Dependence of the faunal composition and species structure of the White Sea littoral Harpacticoida on sediment properties was studied. Three groups of species could be distinguished according to their relationship with sediment properties: (1) species typical of silty sediments, (2) species preferring sediments with high gravel content, and (3) species inhabiting well-sorted washed sands. Vertical distribution of crustaceans within sediments of different types was studied. Vertical migrations of harpacticoids (3) during the tidal cycle were described. Data on interannual variability of harpacticoid fauna are presented.

Gut content of holothurians from the NE Atlantic

This paper describes inter-specific differences in the distribution of sediment in the gut compartments and in the enzyme and bacterial profiles along the gut of abyssal holothurian species - Oneirophanta mutabilis, Psychropotes longicauda and Pseudostichopus villosus sampled from a eutrophic site in the NE Atlantic at different times of the year. Proportions of sediments, relative to total gut contents, in the pharynx, oesophagus, anterior and posterior intestine differed significantly in all the inter-species comparisons, but not between inter-seasonal comparisons. Significant differences were also found between the relative proportions of sediments in both the rectum and cloaca of Psychropotes longicauda and Oneirophanta mutabilis. Nineteen enzymes were identified in either gut-tissue or gut-content samples of the holothurians studied. Concentrations of the enzymes in gut tissues and their contents were highly correlated. Greater concentrations of the enzymes were found in the gut tissues suggesting that they are the main source of the enzymes. The suites of enzymes recorded were broadly similar in each of the species sampled collected regardless of the time of the year, and they were similar to those described previously for shallow-water holothurians. Significant inter-specific differences in the gut tissue concentrations of some of the glycosidases suggest dietary differences. For example, Psychropotes longicauda and Pseudostichopus villosus contain higher levels of chitobiase than Oneirophanta mutabilis. There were no seasonal changes in bacterial activity profiles along the guts of O. mutabilis and Pseudostichopus villosus. In both these species bacterial activity and abundance declined between the pharynx/oesophagus and anterior intestine, but then increased along the gut and became greatest in the rectum/cloaca. Although the data sets were more limited for Psychropotes longicauda, bacterial activity increased from the anterior to the posterior intestine but then declined slightly to the rectum/cloaca. These changes in bacterial activity and densities probably reflect changes in the microbial environment along the guts of abyssal holothurians. Such changes suggest that there is potential for microbial breakdown of a broader range of substrates than could be otherwise be achieved by the holothurian itself. However, the present study found no evidence for sedimentary (microbial) sources of hydrolytic enzymes.

Ensemble prediction distribution maps of macroalgae for current conditions and four climate change scenarios and high resultion bathymetry for Potter Cove, WAP, Antarctica

Species distribution models (SDM) predict species occurrence based on statistical relationships with environmental conditions. The R-package biomod2 which includes 10 different SDM techniques and 10 different evaluation methods was used in this study. Macroalgae are the main biomass producers in Potter Cove, King George Island (Isla 25 de Mayo), Antarctica, and they are sensitive to climate change factors such as suspended particulate matter (SPM). Macroalgae presence and absence data were used to test SDMs suitability and, simultaneously, to assess the environmental response of macroalgae as well as to model four scenarios of distribution shifts by varying SPM conditions due to climate change. According to the averaged evaluation scores of Relative Operating Characteristics (ROC) and True scale statistics (TSS) by models, those methods based on a multitude of decision trees such as Random Forest and Classification Tree Analysis, reached the highest predictive power followed by generalized boosted models (GBM) and maximum-entropy approaches (Maxent). The final ensemble model used 135 of 200 calculated models (TSS > 0.7) and identified hard substrate and SPM as the most influencing parameters followed by distance to glacier, total organic carbon (TOC), bathymetry and slope. The climate change scenarios show an invasive reaction of the macroalgae in case of less SPM and a retreat of the macroalgae in case of higher assumed SPM values.

(Table 1) Content of sand fraction components in sediments of the interglacial MIS 7 optimum from Core LV28-42-4

Distribution of diatoms, radiolarians, planktonic and benthic foraminifers, and sediment components in fraction >0.125 mm was analyzed in a core obtained from the central Sea of Okhotsk within frameworks of the Russian-German KOMEX Project. The core section characterizes the period 190-350 ka, which corresponds to marine-isotopic stages (MIS) 7 to 10. During glacial MIS 10 and MIS 8, the basin accumulated terrigenous material lacking microfossils or containing them in low abundance, which reflects, along with their composition, heavy sea-ice conditions, suppressed bioproductivity, and bottom environment aggressive toward calcium carbonate. Interglacial MIS 9 was characterized by elevated bioproductivity with accumulation of diatomaceous ooze during the climatic optimum (328 to 320 ka). Water exchange with the Pacific was maximal from 328 to 324 ka ago. Environment became moderate and close to the present-day one at the end of the optimum exhibiting possible existence of a dichothermal layer with substantial amounts of surface Pacific water still flowing into the basin. Similar to interglacial MIS 5e and MIS 1, ''old'' Pacific water determined near-bottom environment in the central Sea of Okhotsk during that period, although influx of terrigenous material was higher, probably reflecting more humid climate of the region. Slight warming marked the terminal MIS 8 (approximately 260 ka ago). Paleoceanographic situation during the interglacial MIS 7 was highly variable: from warm-water to almost glacial. The main climatic optimum of MIS 7 occurred within 220-210 ka, when subsurface stratification increased and the dichothermal layer developed. Bottom environment during the studied time interval, except for the optimum of interglacial MIS 9, resembled those characteristic of glacial periods: actively formed ''young'' Okhotsk water displaced ''old'' Pacific deep water.

(Table 1) Deuterium ratios, chloride content and hydrate volume of pore water and hydrate meltwater from ODP Site 164-996

Site 996 is located above the Blake Diapir where numerous indications of vertical fluid migration and the presence of hydrate existed prior to Ocean Drilling Program (ODP) Leg 164. Direct sampling of hydrates and visual observations of hydrate-filled veins that could be traced 30-40 cm along cores suggest a connection between fluid migration and hydrate formation. The composition of pore water squeezed from sediment cores showed large variations due to melting of hydrate during core recovery and influence of saline water from the evaporitic diapir below. Analysis of water released during hydrate decomposition experiments showed that the recovered hydrates contained significant amounts of pore water. Solutions of the transport equations for deuterium (d2H) and chloride (Cl-) were used to determine maximum (d2H) and minimum (Cl-) in situ concentrations of these species. Minimum in situ concentrations of hydrate were estimated by combining these results with Cl- and d2H values measured on hydrate meltwaters and pore waters obtained by squeezing of sediments, by the means of a method based on analysis of distances in the two-dimensional Cl- d2H space. The computed Cl- and d2H distribution indicates that the minimum hydrate amount solutions are representative of the actual hydrate amount. The highest and mean hydrate concentrations estimates from our model are 31% and 10% of the pore space, respectively. These concentrations agree well with visual core observations, supporting the validity of the model assumptions. The minimum in situ Cl- concentrations were used to constrain the rates of upward fluid migration. Simulation of all available data gave a mean flow rate of 0.35 m/k.y. (range: 0.125-0.5 m/k.y.).

Physical Characteristics and Estimated Hydraulic Conductivity from Grain-Size Distribution for Vashon Till, South-Central Puget Lowland, Washington

Population growth, urban development, and increased commercial and industrial activity in the south-central Puget Lowlands of Washington State has led to an increased demand for groundwater. The Vashon till is a glacially consolidated, low-permeability unit comprising unstratified clay, silt, cobbles and boulders with ubiquitous coarse-grained lenses and is an extensive surficial unit throughout the south-central Puget Lowland. Thus, understanding the physical and hydrological characteristics – specifically, the hydraulic conductivity – of this unit is a necessary component of a groundwater model. This study provides (1) a record of the physical characteristics of Vashon till deposits within the study area; and (2) an estimate of the highest, lowest, and average value of saturated hydraulic conductivity based on the grain-size distribution of Vashon till samples collected from six field sites in the Puyallup River Watershed. Analysis shows that the average moisture content ranges between about 1 and 6%, average dry bulk density is about 2.20 g/cm3, and average porosity is about 17%. Grain-size distributions show that half of the samples analyzed are well graded, while the other half is poorly graded. Grain-size distributions also show an average d10 value of about 0.20 mm, and average ff values ≤ 16%, which are key values in estimating the saturated hydraulic conductivity of over-consolidated glacial deposits. Based on these observed values, the estimates of hydraulic conductivity range from a minimum of 0.02 m/d to a maximum of 1.38 m/d in within the general Vashon till.

Determining Spatial Distribution and Physical Properties of the Vashon Advance Outwash near Mountlake Terrace, Washington

This study is aimed at determining the spatial distribution, physical properties, and groundwater conditions of the Vashon advance outwash (Qva) in the Mountlake Terrace, WA area. The Qva is correlative with the Esperance Sand, as defined at its type section; however, local variations in the Qva are not well-characterized (Mullineaux, 1965). While the Qva is a dense glacial unit with low compressibility and high frictional shear strength (Gurtowski and Boirum, 1989), the strength of this unit can be reduced when it becomes saturated (Tubbs, 1974). This can lead to caving or flowing in excavations, and on a larger scale, can lead to slope failures and mass-wasting when intersected by steep slopes. By studying the Qva, we can better predict how it will behave under certain conditions, which will be beneficial to geologists, hydrogeologists, engineers, and environmental scientists during site assessments and early phases of project planning. In this study, I use data from 27 geotechnical borings from previous field investigations and C-Tech Corporation’s EnterVol software to create three-dimensional models of the subsurface geology in the study area. These models made it possible to visualize the spatial distribution of the Qva in relation to other geologic units. I also conducted a comparative study between data from the borings and generalized published data on the spatial distribution, relative density, soil classification, grain-size distribution, moisture content, groundwater conditions, and aquifer properties of the Qva. I found that the elevation of the top of the Qva ranges from 247 to 477 ft. I found that the Qva is thickest where the modern topography is high, and is thinnest where the topography is low. The thickness of the Qva ranges from absent to 242 ft. Along the northern, east-west trending transect, the Qva thins to the east as it rises above a ridge composed of Pre- Vashon glacial deposits. Along the southern, east-west trending transect, the Qva pinches out against a ridge composed of pre-Vashon interglacial deposits. Two plausible explanations for this ridge are paleotopography and active faulting associated with the Southern Whidbey Fault Zone. Further investigations should be done using geophysical methods and the modeling methods described in this study to determine the nature of this ridge. The relative density of the Qva in the study area ranges from loose to very dense, with the loose end of the spectrum probably relating to heave in saturated sands. I found subtle correlations between density and depth. Volumetric analysis of the soil groups listed in the boring logs indicate that the Qva in the study area is composed of approximately 9.5% gravel, 89.3% sand, and 1.2% silt and clay. The natural moisture content ranges from 3.0 to 35.4% in select samples from the Qva. The moisture content appears to increase with depth and fines content. The water table in the study area ranges in elevation from 231.9 to 458 ft, based on observations and measurements recorded in the boring logs. The results from rising-head and falling-head slug tests done at a single well in the study area indicate that the geometric mean of hydraulic conductivity is 15.93 ft/d (5.62 x 10-03 cm/s), the storativity is 3.28x10-03, and the estimated transmissivity is 738.58 ft2/d in the vicinity of this observation well. At this location, there was 1.73 ft of seasonal variation in groundwater elevation between August 2014 and March 2015.

An investigation of contaminant distribution in subsurface marine wood waste at Mill-A South Terminal, Port of Everett, Washington

This project investigates the correlation between contaminants and the wood waste present in marine sediments off the shore of the Port of Everett in the former Weyerhaeuser Mill-A pulp mill site. The investigation includes the results of two field studies, which tested contaminant levels in 22 boreholes as well as several surface samples. The contaminants include heavy metals and wood waste byproducts. These results, along with 14 other bore logs, provide the framework for a three-dimensional site model, interpolating the full extent of the depositional units and organic and inorganic chemicals found at Mill-A. The sediments of interest are divided into five depositional units defined by the percent wood content and type of wood: native material (<5% wood), intermediate (<30% wood), sawdust (<30% wood), woodchips (<30% wood), and poorly sorted sands with silt (SM-SP) (0% wood). The contaminants include arsenic, 2,4-dimethylphenol, and total organic carbon. Three-dimensional modeling software, RockWorks, interpolated the discrete borehole data of sediment and contaminants assuming horizontal continuity between sampling locations. The sediment distribution was calculated within concentration ranges for each contaminant of concern. The lowest detection limits, the screening levels, and the cleanup levels defined these ranges. Total organic carbon served as a proxy to estimate the quantity of wood waste in the sediment. As a known byproduct of wood decomposition, 2,4-dimethylphenol was expected to be more prevalent in the depositional units with more wood waste. Finally, arsenic was a proxy for other contaminants to determine if contaminants at Mill-A are dominant in sediments with high percentages of wood waste. The volumetric distribution established that high levels of total organic carbon are present in the sediment with higher percentages of wood waste. This correlation was stronger in the decomposing sawdust-rich sediment than the woodchip-rich sediment. The 2,4-dimethylphenol concentrations above cleanup standards were dominant in the sawdust-rich, intermediate and native sediments. Concentrations of 2,4-dimethylphenol below cleanup levels characterized the native sediment. The distribution of arsenic showed no statistically significant correlation to wood content in sediment. These results do not support the hypothesis of contaminant-rich wood waste, as many of the high concentrations of contaminants were not in the wood-rich sediments. This suggests that the contaminants are more distributed among all depositional units at Mill-A rather than focused within sediments with a high percent of wood waste. Understanding the distribution of potentially toxic compounds with wood waste is important for restoring the Puget Sound waterways to a more habitable environment. Future studies should include new data to validate these results and to limit the uncertainty of the extent of contaminants. Future studies may also find motive in looking for a correlation between contaminants and grain size based on previous studies linking these characteristics. These investigations will benefit the current cleanup effort as well as future cleanup efforts at similarly contaminated waterways.

Mollusca Non-Grata: The influence of top-down control and residence time on the abundance, distribution, and behavior of non-native marine snails in Washington State

Thesis (Ph.D.)--University of Washington, 2016-06

The effect of the reduction of carbon content on the toughness of high chromium white irons in the as-cast state

Three high chromium white cast irons were examined in the as-cast state to determine the effect of the carbon content on the fracture toughness. The plane strain fracture toughness K-Ic and the fracture strength were measured for each alloy. X-ray mapping was used to identify the phases on the fracture surfaces. Scanning electron fractography and optical microscopy were used to determine the volume fraction of each phase on the fracture surfaces. It was found that most fracture occurred in the eutectic carbides, but that for the alloys with a reduced volume fraction of eutectic carbides, a small amount of crack propagation occurred in the austenitic dendrites. This change in crack path correlated with an increase in fracture toughness. The Ritchie-Knott-Rice model of brittle fracture was applied. It was found to sensibly predict the critical length for fracture for each alloy. Deep etching was employed to examine the distribution of eutectic carbides. It was found that the eutectic carbides formed a continuous network in each case. (C) 2004 Kluwer Academic Publishers.