Gain of chromosome 3q defines the transition from severe dysplasia to invasive carcinoma of the uterine cervix.

We have chosen tumors of the uterine cervix as a model system to identify chromosomal aberrations that occur during carcinogenesis. A phenotype/genotype correlation was established in defined regions of archived, formalin-fixed, and hematoxylin/eosin-stained tissue sections that were dissected from normal cervical epithelium (n = 3), from mild (n = 4), moderate (n = 6), and severe dysplasias/carcinomas in situ (CIS) (n = 13), and from invasive carcinomas (n = 10) and investigated by comparative genomic hybridization. The same tissues were analyzed for DNA ploidy, proliferative activity, and the presence of human papillomavirus (HPV) sequences. The results show that an increase in proliferative activity and tetraploidization had occurred already in mildly dysplastic lesions. No recurrent chromosomal aberrations were observed in DNA extracted from normal epithelium or from mild and moderate dysplasias, indicating that the tetraploidization precedes the loss or gain of specific chromosomes. A gain of chromosome 3q became visible in one of the severe dysplasias/CIS. Notably, chromosome 3q was overrepresented in 90% of the carcinomas and was also found to have undergone a high-level copy-number increase (amplification). We therefore conclude that the gain of chromosome 3q that occurs in HPV16-infected, aneuploid cells represents a pivotal genetic aberration at the transition from severe dysplasia/CIS to invasive cervical carcinoma.

Contactus adherens, a special type of plaque-bearing adhering junction containing M-cadherin, in the granule cell layer of the cerebellar glomerulus.

In the glomeruli of the granule cell layer of mammalian cerebellum, neuronal extensions are interconnected by numerous small, nearly isodiametric (diameters up to 0.1 micron), junctions previously classified as puncta adherentia related to the vinculin-containing, actin microfilament-anchoring junctions of the zonula adherens of epithelial and certain other cells. Using immunofluorescence and immunoelectron microscopy, we have found, however, that these junctions are negative for E- and VE-cadherin, for desmosomal cadherins, and also for vinculin, alpha-actinin, and desmoplakin, but they do contain, in addition to the protein plakoglobin common to all forms of adhering junctions, the plaque proteins alpha- and beta-catenin and the transmembrane glycoprotein M-cadherin previously found as a spread--i.e., not junction bound--plasma membrane protein in certain fetal and regenerating muscle cells and in satellite cells of adult skeletal muscle. We conclude that these M-cadherin-containing junctions of the granule cell layer represent a special type of adhering junction, for which we propose the term contactus adherens (from the Latin contactus, for touch, site of bordering upon, also influence), and we discuss the differences between the various adhering junctions on the basis of their molecular constituents.

Mathematical modelling of the lower urinary tract

The lower urinary tract is one of the most complex biological systems of the human body as it involved hydrodynamic properties of urine and muscle. Moreover, its complexity is increased to be managed by voluntary and involuntary neural systems. In this paper, a mathematical model of the lower urinary tract it is proposed as a preliminary study to better understand its functioning. Furthermore, another goal of that mathematical model proposal is to provide a basis for developing artificial control systems. Lower urinary tract is comprised of two interacting systems: the mechanical system and the neural regulator. The latter has the function of controlling the mechanical system to perform the voiding process. The results of the tests reproduce experimental data with high degree of accuracy. Also, these results indicate that simulations not only with healthy patients but also of patients with dysfunctions with neurological etiology present urodynamic curves very similar to those obtained in clinical studies.

Water-quality assessment of the Lower Susquehanna River Basin, Pennsylvania and Maryland : sources, characteristics, anaylsis, and limitations of nutrient and suspended-seditment data, 1975-90 /

"National Water-Quality Assessment Program"

Certain aboriginal remains of the Black Warrior River : certain aboriginal remains of the lower Tombigbee river : certain aboriginal remains of Mobile Bay and Mississippi Sound ; miscellaneous investigation in Florida /

Volumes are paged continuously.

Value of flood height reduction from Tennessee valley authority reservoirs to the alluvial valley of the lower Mississippi river.

"This report has been prepared by Charles W. Okey, principal civil engineer on the staff of the Tennessee vally authority."--p.v.

The Roman Wall : an historical and topographical description of the barrier of the lower isthmus extending from the Tyne to the Solway deduced from numerous personal surveys /

Interleaved with many insertions and manuscript notes ; partly mutilated.

Conference in the matter of Pollution of the Interstate Waters of the Lower Mississippi River : proceedings : May 5-6, 1964, New Orleans, Louisiana.

"Held under the provisions of Section 8 of the Federal Water Pollution Control Act"--v. 1, p. 3.

The hand-book to the Roman wall: a guide to tourists traversing the barrier of the lower isthmus.

Mode of access: Internet.

On the Theraphosidae of the lower Amazons: being an account of the new genera and species of this group of spiders discovered during the expedition of the steamship 'Faraday' up the river Amazons.

Reprinted from the Proceedings of the Zoological Society of London, June 16, 1896.

Cathlamet on the Columbia; recollections of the Indian people and short stories of early pioneer days in the valley of the lower Columbia River,

Mode of access: Internet.

Periodicals in the Central library as currently received in the Periodical reading room and kept bound on the shelves of the Lower hall and Bates hall.

Caption title.

A Pageant of the lower Cape Fear,

Designed and written by members of the North Carolina Sorosis.

Report on condition of the lower classes of population in Bengal.

Mode of access: Internet.