A glycine-arginine domain in control of the human MRE11 DNA repair protein.

Human MRE11 is a key enzyme in DNA double-strand break repair and genome stability. Human MRE11 bears a glycine-arginine-rich (GAR) motif that is conserved among multicellular eukaryotic species. We investigated how this motif influences MRE11 function. Human MRE11 alone or a complex of MRE11, RAD50, and NBS1 (MRN) was methylated in insect cells, suggesting that this modification is conserved during evolution. We demonstrate that PRMT1 interacts with MRE11 but not with the MRN complex, suggesting that MRE11 arginine methylation occurs prior to the binding of NBS1 and RAD50. Moreover, the first six methylated arginines are essential for the regulation of MRE11 DNA binding and nuclease activity. The inhibition of arginine methylation leads to a reduction in MRE11 and RAD51 focus formation on a unique double-strand break in vivo. Furthermore, the MRE11-methylated GAR domain is sufficient for its targeting to DNA damage foci and colocalization with gamma-H2AX. These studies highlight an important role for the GAR domain in regulating MRE11 function at the biochemical and cellular levels during DNA double-strand break repair.

COMP3016 Web Technology - Strand 3 "History" Lecture 1

Lecture 1: The Pioneers and History of Hypertext (pre-WWW) Contains Powerpoint Lecture slides and Hypertext Research Papers: Bush: As We may Think; Engelbart: NLS and A Framework for Augmenting Human Intelligence; Nelson: Xanalogical Structure; Conklin: A Survey of Hypertext; Halasz 1987: Reflections on NoteCards: Seven Issues for the Next Generation of Hypermedia Systems; Berners-Lee 1994 The World-Wide Web.

COMP3016 Web Technology - Strand 3 "History" Lecture 2

Lecture 1: Contributions of Pre WWW Research: Open Hypermedia Systems Contains Powerpoint Lecture slides and Hypertext Research Papers: Industrial Strength Hypermedia: Requirements for a Large Engineering Enterprise (Malcolm et al. 1991); Towards An Integrated Information Environment With Open Hypermedia Systems (Davis et al. 1992); Unifying Strategies for Web Augmentation (Bouvin 1999); Hyper-G (Adapted from Lowe and Hall); OHP:A Draft Proposal for a Standard Open Hypermedia Protocol (Davis et al. 1996); XML Linking (DeRose 99)

COMP3016 Web Technology - Strand 3 "History" Lecture 3

Lecture 3: Contributions of Pre WWW Research: Spatial Hypertext and Temporal Hypertext Contains Powerpoint Lecture slides and Hypertext Research Papers: Spatial [SPATIAL] VIKI: spatial hypertext supporting emergent structure (Marshall, 94); Towards Geo-Spatial Hypermedia: Concepts and Prototype Implementation, (Gronbaek et al. 2002); Cyber Geography and Better Search Engines; [TEMPORAL] Anticipating SMIL 2.0: The Developing Cooperative Infrastructure for Multimedia on the Web (Rutledge 1999); Its About Time: Link Streams as Continuous Metadata (Page et al., 2001); Everything You Wanted to Know About MPEG-7:Part 1 (Nack & Lindsay 1999)

COMP3016 Web Technology - Strand 3 "History" Lecture 4

Lecture 4: Ontological Hypertext and the Semantic Web Contains Powerpoint Lecture slides and Hypertext Research Papers: Conceptual linking: Ontology-based Open Hypermedia (Carr et al. 2001); CS AKTiveSpace: Building a Semantic Web Application (Glaser et al., 2004); The Semantic Web Revisited (Shadbolt, Hall and Berners-Lee, 2006); Mind the Semantic Gap (Millard et al., 2005).

COMP3016 Web Technology - Strand 3 "History" Lecture 5

Lecture 5: Web 2.0 and Social Hypertext Contains Powerpoint Lecture slides and Hypertext Research Papers: What Is Web 2.0 Design Patterns and Business Models for the Next Generation of Software . Tim O'Reilly (2005); Web 2.0: Hypertext by Any Other Name? (Millard & Ross, 2006)

COMP3016 Web Technology - Strand 3 "History" Lecture 6

Lecture 6: Where are all the links taking us: Web Science Contains Powerpoint Lecture slides and Hypertext Research Papers: The Literati (The Cyberspace and critical theory website) (Eastgate website); Pervasive Hypertext at Southampton and at Aarhus; Adaptive Hypertext - The Next Big Thing: (De Bra & Chepegin, 2004); Web Science: Creating a Science of the Web (Berners-Lee, Hall, Hendler, Shadbolt & Weitzner, 2006).

COMP3016 Web Technology - Strand "XML" Lecture 1

Lecture 1: Basic XML & HTML5 Lecture slides and exercises for reading and writing basic XML (without DTDs).

COMP3016 Web Technology - Strand "XML" Lecture 2

Lecture 1: DTDs and XML Structure Verification Lecture slides and exercises for validating XML with DTDs.

COMP3016 Web Technology - Strand "XML" Lecture 3

Lecture 3: DOM and XPath Lecture slides and exercises for using DOM and XPath to access material within an XML database or document.

COMP3016 Web Technology - Strand "XML" Lecture 5

Lecture 5: XLink and XPointer Lecture slides and exercises for using XLink and XPointer to link to and control material within an XML database or document.

COMP3016 Web Technology - Strand "XML" Lecture 6

Lecture 6: RDF and Metadata Lecture slides and exercises for using RDF to describe Web resources