933 resultados para Mutant Cycles


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Strong diurnal cycles in ambient aerosol mass were observed in a rural region of Southeast Brazil where the trace composition of the lower troposphere is governed mainly by emissions from agro-industry. An optical particle counter was used to record size-segregated aerosol number concentrations between 13 May 2010 and 15 March 2011. The data were collected every 10 min and used to calculate aerosol mass concentrations. Aerosol samples were also collected onto filters during daytime (10:00-16:00 local time) and nighttime (20:00-06:00) periods, for subsequent analysis of soluble ions and water-soluble organic carbon. Biomass burning aerosols predominated during the dry winter, while secondary aerosols were most important in the summer rainy season. In both seasons, diurnal cycles in calculated aerosol mass concentrations were due to the uptake of water by the aerosols and, to a lesser extent, to emissions and secondary aerosol formation. In neither season could the observed mass changes be explained by changes in the depth of the boundary layer. In the summer, nighttime increases in aerosol mass ranged from 2.7-fold to 81-fold, depending on particle size, while in the winter, the range was narrower, from 2.2-fold to 9.5-fold, supporting the possibility that the presence of particles derived from biomass burning reduced the overall ability of the aerosols to absorb water. Key Points Diurnal cycle of agro-industrial aerosol mass governed by humidity Biomass burning emissions act to suppress particle growth Need to consider diurnal mass cycles in aerosol dry deposition models ©2013. American Geophysical Union. All Rights Reserved.

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Paper presented at the LASA Association, 14-16 November 1975, San Francisco, California

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O presente estudo descreve a associação de nematódeos da Baía de Tamandaré (Brasil), praia arenosa tropical típica, durante as marés (baixa, enchente, alta e vazante) de dois ciclos de maré consecutivos, em quatro meses diferentes do ano (Maio, Julho, Setembro e Novembro). A associação de nematódeos foi dominada por Metachromadora e Perepsilonema e variou significativamente entre meses e marés. Densidades foram mais baixas em julho e as mudanças na associação ocorreram durante meses de transição entre chuvoso e seco mostrando a influência do ciclo de chuvas. No ciclo de maré a enchente e vazante pareceram exercer a maior influência, embora os padrões não fossem muito claros. Recomenda-se que os estudos devam ser feitos ao nível de gêneros/espécies para melhor compreensão dos padrões das associações de nematódeos durante ciclos de marés.

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A estrutura populacional (machos, fêmeas e juvenis) e densidade de Mesacanthion hirsutum Gerlach foram estudadas durante as marés (baixa, enchente, alta e vazante) de dois ciclos de maré consecutivos, em quatro meses diferentes do ano (Maio, Julho, Setembro e Novembro). As variações de densidade de Mesacanthion hirsutum mostraram associação com o ciclo de chuvas, com densidades mais baixas durante Julho e Setembro e significativamente maiores em Maio e Novembro. A estrutura populacional constituiu-se em sua maior parte por juvenis indicando uma reprodução continua durante todo o período de estudo. Não foram encontradas diferenças significativas entre os períodos claros e escuros do dia, contudo maiores densidades foram detectadas durante as marés altas e vazantes demonstrando que a espécie pode estar se dispersando através da coluna d'água e/ou migrando dentro do sedimento.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Developing nutritional strategies that can reduce production costs for the fish industry without affecting productive performance is paramount to make the activity sustainable. We investigated if short-term cycles of feed deprivation and refeeding elicit compensatory growth in matrinxa (Brycon amazonicus) juveniles, using three feeding protocols for 60 days (Phase 1): two levels of deprivation (feed deprivation for two days and refeeding for three - D2R3, or four days - D2R4) and a control level (daily feeding). Following, all fish groups were fed daily at satiation for 15 days (Phase 2). At Phase 1, matrinxa achieved full compensatory growth in both deprivation levels by increasing feed intake and feed efficiency. Overall, deprived fish consumed 40% (D2R3) and 36% (D2R4) less feed than fish fed daily. In Phase 2, growth was similar for all fish. Feed intake increased in both deprived fish, but feed efficiency did not differ among groups and was lower than in Phase 1, indicating a reduced efficiency in feed utilization when food was freely available. We propose that intermittent cycles of feeding represent an effective means to reduce production costs. (C) 2014 Elsevier B.V. All rights reserved.

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Feeding strategies that reduce feed and promote compensatory growth could be an interesting tool to reduce costs in the fish production. However, fish health must be monitored to evaluate if their physiological response to adverse conditions, such as parasite infection, does not become compromised. A 12-wk growth trial was conducted to determine the physiological responses of pacu, Piaractus mesopotamicus, that were subjected to different fasting/refeeding cycles and infected with the Dolops carvalhoi. The schemes were: (i) control group fish (FD), (ii) food-restricted and controlled refeeding group (FR/Rc), and (iii) food-restricted and refeeding to satiation group (FR/Rs). After 84 d, the fish were exposed to D. carvalhoi for 30 h. The fish subjected to food restriction did not exhibit compensatory growth. Cortisol levels decreased in all groups within 30 h after infection. Glucose levels increased 6 h after the D. carvalhoi in the FR/Rs and 30 h after infection in the FD. In all of the fish groups, the hematocrit values were reduced after infection, and it was associated with a reduction in the mean corpuscular volume and erythrocytes. At 30 h after infection, the number of erythroblasts increased. The use of the feeding schemes does not indicate a failure of the pacu physiological responses.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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We determine the relation amongst the global Lê cycles and the Milnor classes of analytic hypersurfaces defined by a section of a very ample line bundle over a compact complex manifold. The key point is finding appropriate expressions for the global Lê cycles and for the Milnor classes in terms of polar varieties. Our starting points are an interpretation of the Lê cycles given by T. Gaffney and R. Gassler, a formula by A. Parusinski and P. Pragacz for the Milnor classes via McPherson’s functor, and a conjecture of J.-P. Brasselet, that we prove, stating that Milnor classes can be expressed in terms of polar varieties. We then use the work by R. Piegne for Mather classes, by J. Schürmann and M. Tibăr for MacPherson’s classes for constructible functions, and by D. Massey for an extension of the local Lê cycles for constructible sheaves.

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Glycogen functions as a carbohydrate reserve in a variety of organisms and its metabolism is highly regulated. The activities of glycogen synthase and glycogen phosphorylase, the rate-limiting enzymes of the synthesis and degradation processes, respectively, are regulated by allosteric modulation and reversible phosphorylation. To identify the protein kinases affecting glycogen metabolism in Neurospora crassa, we performed a screen of 84 serine/threonine kinase knockout strains. We identified multiple kinases that have already been described as controlling glycogen metabolism in different organisms, such as NcSNF1, NcPHO85, NcGSK3, NcPKA, PSK2 homologue and NcATG1. In addition, many hypothetical kinases have been implicated in the control of glycogen metabolism. Two kinases, NcIME-2 and NcNIMA, already functionally characterized but with no functions related to glycogen metabolism regulation, were also identified. Among the kinases identified, it is important to mention the role of NcSNF1. We showed in the present study that this kinase was implicated in glycogen synthase phosphorylation, as demonstrated by the higher levels of glycogen accumulated during growth, along with a higher glycogen synthase (GSN) ±glucose 6-phosphate activity ratio and a lesser set of phosphorylated GSN isoforms in strain Ncsnf1KO, when compared with the wild-type strain. The results led us to conclude that, in N. crassa, this kinase promotes phosphorylation of glycogen synthase either directly or indirectly, which is the opposite of what is described for Saccharomyces cerevisiae. The kinases also play a role in gene expression regulation, in that gdn, the gene encoding the debranching enzyme, was down-regulated by the proteins identified in the screen. Some kinases affected growth and development, suggesting a connection linking glycogen metabolism with cell growth and development.