Mouse mesenchymal stem cells inhibit high endothelial cell activation and lymphocyte homing to lymph nodes by releasing TIMP-1.

Mesenchymal stem cells (MSC) represent a promising therapeutic approach in many diseases in view of their potent immunomodulatory properties, which are only partially understood. Here, we show that the endothelium is a specific and key target of MSC during immunity and inflammation. In mice, MSC inhibit activation and proliferation of endothelial cells in remote inflamed lymph nodes (LNs), affect elongation and arborization of high endothelial venules (HEVs) and inhibit T-cell homing. The proteomic analysis of the MSC secretome identified the tissue inhibitor of metalloproteinase-1 (TIMP-1) as a potential effector molecule responsible for the anti-angiogenic properties of MSC. Both in vitro and in vivo, TIMP-1 activity is responsible for the anti-angiogenic effects of MSC, and increasing TIMP-1 concentrations delivered by an Adeno Associated Virus (AAV) vector recapitulates the effects of MSC transplantation on draining LNs. Thus, this study discovers a new and highly efficient general mechanism through which MSC tune down immunity and inflammation, identifies TIMP-1 as a novel biomarker of MSC-based therapy and opens the gate to new therapeutic approaches of inflammatory diseases.

Evaluation of freeway high occupancy vehicle lanes and ramp metering. Volume I. Final report.

Transportation Department, Office of the Assistant Secretary for Policy and International Affairs, Washington, D.C.

Evaluation of freeway high occupancy vehicle lanes and ramp metering. Volume II: bibliography and abstracts.

Transportation Department, Office of the Assistant Secretary for Policy and International Affairs, Washington, D.C.

Oversight hearing on Council on Wage and Price Stability : hearing before the Subcommittee on Economic Stabilization of the Committee on Banking, Finance and Urban Affairs, House of Represenatives, Ninety-sixth Congress, first session, October 10, 1979.

"Serial no. 96-33."

The Bath physicians of former times. A paper read before the Bath Literary and Philosophical Association, October 20th, 1882.

Mode of access: Internet.

Literary institutions : their relation to public opinion : an essay written for the London Literary and Scientific Institution.

Mode of access: Internet.

Forms of judgments and orders in the high court of justice and court of appeal : having especial reference to the Chancery division, with practical notes /

Includes bibliographical references and index.

Wet-weather high-hazard locations - identification and evaluation. Final report.

Federal Highway Administration, Washington, D.C.