Impact of the processes of total testicular regression and recrudescence on the epididymal physiology of the bat myotis nigricans (chiroptera: vespertilionidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Morphometric evaluation of the rat testis, epididymis and vas deferens following chemical sympathectomy with guanethidine

Selective chemical sympathectomy of the internal sex organs of adult male rats was undertaken by long term administration of low doses of guanethidine. The spermatogenic activity of the testis was unaffected by treatment. Examination of the vas deferens using morphometric methods revealed a marked increase in luminal area in contrast to a decrease in muscle layer area and in epithelial height. This is morphological evidence of sperm accumulation caused by a disorder in ductal contractile activity. No structural changes were observed in the epididymis. However, the concentration of spermatozoa in the sperm suspension stored in the cauda epididymidis was significantly increased in denervated rats. This result is discussed in terms of a sympathetic control of resorption mechanisms in the epididymis.

Study on the origin and nature of the adenomatoid odontogenic tumor by immunohistochemistry

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Immunolocalization of aquaporins 1 and 9 in the ram efferent ducts and epididymis

Aquaporins (AQPs) are essential membrane protein channels for the transport of water across membranes. Fluid movement in the epididymis is important for modulation of the luminal environment, in which sperm mature and reside. This study was designed to understand the morphology and localization of AQPs in ram efferent ducts (ED) and epididymis. For this purpose, the epididymis of seven animals were removed for histologic and immunohistochemical analyses. AQP1 immunoreactivity was observed in the apex of the ED, and AQP9 was found adjacent to the nuclei of the epithelial cells of the ED. The epithelial lining of ram epididymis is pseudostratified columnar and presents principal, basal, apical and narrow cells. In the initial segment (IS), a moderate reaction for AQP1 was observed in the apical cytoplasm of epithelial cells. An intense reactivity for AQP1 was noted over the microvilli of principal cells and in spermatozoa in the caput. In the corpus and cauda, AQP1 was noted only over the endothelial cells of vascular channels located in intertubular spaces. A weak-to-moderate reaction for AQP9 was observed in the nuclei of epithelial cells in the IS, caput and corpus of the epididymis. In the cauda, an intense reaction to AQP9 was observed in the epithelial border. In the IS, caput and corpus, the reactivity for AQP9 differed from those observed in domestic animals. The cauda showed a pattern similar to that previously described. These results indicate that AQPs 1 and 9 have reversed locations and roles in rams, suggesting activity variations related with fluid and solute absorption throughout the epididymis.

Ultrastructure of the digestive tract in neotropical carnivorous catfish Hemisorubim platyrhynchos (Valenciennes, 1840) (Siluriformes, Pimelodidae)

The surface of the digestive tract of Hemisorubim platyrhynchos was analyzed by scanning electron microscopy. Morphometric studies by transmission electron microscopy were performed to analysis the intestinal microvilli. H. platyrhynchos is a Neotropical carnivorous freshwater catfish featuring a short digestive tract composed of a short esophagus, saccular stomach, and intestine with four regions: anterior, middle, posterior, and rectal. The esophageal surface is constituted by fingerprint-like microridges that anchor the mucosubstances secreted by goblet cells facilitating the passage of food. Goblet cells present the opening to the esophageal lumen, between the microridges. Club cells are in basal epithelium and they do not present the opening to the lumen. The gastric luminal surface shows polygon-shaped epithelial cells which secrete granules by exocytose to protect the gastric surface. The intestinal luminal surface reveals folds that are thicker in the anterior intestine than in the posterior intestine, increasing the absorptive surface area. The intestinal surface presents the microvilli of enterocytes and the opening of goblet cells. The morphometric analysis showed that the microvilli are longer in the anterior intestine, significantly decreasing towards the posterior intestine. The microvilli surface area significantly is greater in the anterior and middle intestine than in the posterior intestine. Numerous openings of goblet cells were observed in the posterior intestine acting in epithelial protection and lubrication. SCANNING 9999:1-8, 2015. © 2015 Wiley Periodicals, Inc.

Eferocitose na presença de PAMP estimula ativação de macrófagos de perfil misto M1/M2

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Predição genômica para o peso ao sobreano via norma de reação em bovinos de corte

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Avaliação da atividade da unidade epidermo-melânica e do dano dérmico no melasma

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A herança genotípica proposta por Wilhelm Ludwig Johannsen

Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq)

Investigação sobre a inclusão do episódio histórico da teoria genotípica de Wilhelm Ludwig Johannsen na formação inicial de professores e pesquisadores

This paper is part of a broader investigation on Epistemology of Biology carried out by a group of researchers from Cascavel/PR. It involves studies and research about biological thought on subjects such as the growing of the concepts of gene, genotype and phenotype. The aim of this research was to gather the undergraduate students of Biological Sciences’ views about the role played by the inclusion of a historical episode in the study group as a trigger for epistemological discussions and didactic reflections. Firstly, the students received Johannsen’s article “The genotype conception of heredity”, published in 1911 in the journal The American Naturalist, in which he proposed the concepts of genotype and phenotype, among other ones. After, there was a discussion held by group about the concepts proposed in the article taking into account the epistemological, historical and didactic aspects. Data collection occurred through the recording of the group discussions and individual interviews. The results suggest that the inclusion of this historical episode, enabled the participants to develop critical thinking through collective reflection on the meaning of biological knowledge in different contexts.

Uma investigação com graduandos de licenciatura em Ciências Biológicas sobre a relação genotipo fenotipo na perspectiva da epistemologia de Gaston Bachelard

This work aims presenting and discussing the conceptual (re)construction of the Biological Sciences undergraduate students on the genotype-phenotype relationship. Data collection occurred through a group of researchers systematic monitoring, at weekly discussion meetings. The data were analyzed and discussed based on Bachelard epistemology foundations. The analysis of the results suggests that the inclusion of discussions of historical and epistemological issues, in that research group, favored reflections that contributed to the expansion of the thinking about the genotype-phenotype relationship.

Estudo genético de populações de soja com fonte de resistência à Heterodera glycines (raça 3) oriundas de topocruzamentos tipo alimento x tipo grão

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Seleção e associação genômica para precocidade sexual em bovinos da raça Nelore

Pós-graduação em Genética e Melhoramento Animal - FCAV

Estudo de genes de Candida albicans com função desconhecida quanto à formação de biofilme, características biológicas e interação patógeno- hospedeiro

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Leveduras patogênicas em fezes de aves de cativeiro: relação fenotípica e genotípica entre os isolados

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)