Caracterização físico-mecânica de painéis de partículas de bambu com adição de casca de café

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A substituição do meio TCM pelo meio BARC, suplementado com SFB, BSA ou PVA, para maturação de oócitos bovinos in vitro, não incrementa a subsequente produção de blastocistos

This study was carried out to assess the influence of bovine embryo culture medium Beltsville Agriculture Research Center (BARC), supplemented with FCS, BSA or PVA, on the in vitro oocyte maturation, evidenced by cleavage rate and blastocysts production at different developmental stages. Three experiments were performed, as follows: exp.1: addition of FCS to BARC medium at concentrations of 0, 5 and 10%; exp. 2: addition of BSA to BARC medium at concentrations of 0, 4 and 8 mg/ml; exp. 3: addition of PVA to BARC medium at concentrations of 0, 0.5 and 1.0 mg/ml. TCM 199 supplemented with bicarbonate, pyruvate, gentamicin sulfate, FSH, LH and FCS was used as control group. Oocytes obtained from cow ovaries at slaughterhouse were selected in PBS, and then matured in BARC medium supplemented with FSH, LH and gentamicin sulfate, according to the experimental design. Percoll gradient was used for sperm selection and TALP medium for IVF. In vitro embryo culture was in SOF-m medium; a humidified atmosphere with 5% CO2, in air, at 38.7oC was used for all steps. The number of oocytes reaching blastocyst, expanded blastocyst, and hatched blastocyt stages was recorded, respectively at 72 and 168 h post-insemination. ANOVA and Bonferroni t test were used to determine differences among groups. Differences of P<0.05 were taken as significant. Higher percentage (P<0.05) of cleaved oocytes was observed in group TCM + FCS than for the other groups matured in BARC supplemented with FCS or BSA, regardless the concentration used. However, the cleavage rate was similar between groups BARC plus PVA with 1 mg/ml (85.7%) and TCM + FCS (90.8%). Significant difference was found among groups for the production of blastocysts, with the control group yielding a higher number of blastocysts (results ranging from 47.4 to 51.4%, in comparison with groups using BARC + FCS (4.1 to 19.7%), BSA (1.4 to 5.6%) and PVA (5.7 to 10.6%). In conclusion, BARC medium supplemented with different macromolecules did not promote a beneficial effect on in vitro oocyte maturation, resulting in lower rate of cleavage and blastocyst production when compared with TCM + FCS medium.

Efeitos da adição do IGF-1 ou IGF-LongR3 sobre aspectos celulares e moleculares de complexos cumulus-oócito durante a maturação oocitária in vitro em bovinos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização física e química da carne de peito de aves de diferentes idades submetida à maturação

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito da adição de vesículas extracelulares intrafoliculares de vacas holandesas submetidas ao estresse térmico em meio de maturação oocitária in vitro

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Adição de ácido linolênico e L-carnitina na maturação oocitária: efeitos sobre o metabolismo celular, potencial de desenvolvimento e criotolerância de embriões bovinos produzidos in vitro

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Criança não é manga, não amadurece: conceito de maturação na teoria histórico-cultural

The idea of "mature development" is based, frequently, on explanations of school failure: the student does not learn because he is immature and the school has to wait until he gets "mature". When one says that a child is not mature compared to the development already attained by an adult, one focuses only the quantitative differences between them and forgets that these new qualities of the adult did not arise by the maturation, but by the permanent appropriation process of the human culture. Thus, this idea of "maturity of development" expresses a deep biologization of the human being, reducing social and educational problems explanation to the biological apparatus of the individual. The purpose of this essay is to analyze the relationship between maturation and development, pointing out the limits of biologists’ explanations of human phenomena and the possibilities of explanation formulated by the historical-cultural theory to the organization of pedagogical work. This concept gives a new configuration to the role of maturation in the learning process and gives the school education a central role in the development of higher psychological functions. Thus, the school does not have to wait for the child’s maturation. Rather, it is its duty to create conditions for his/her maturation to become effective.

Aproveitamento do resíduo do processamento do café na composição de painéis MDP

A produção de produtos derivados de madeira e bambu vem crescendo nos últimos anos na busca de um aproveitamento mais racional dessas matériasprimas. Este trabalho tem por finalidade, o estudo da aplicação de resíduos do processamento do café em um painel MDP. Os painéis MDP foram produzidos com partículas de bambu da espécie Dendrocalamus giganteus e adição de partículas de casca de café na camada intermediária do painel, unidas por resina poliuretana à base de mamona. A caracterização físico-mecânica foi realizada baseando-se nas especificações da NBR 14810-3 (2006). Os resultados obtidos foram comparados com a norma brasileira NBR 14810-2 (2006), resultando em valores próximos aos comercialmente encontrados.

Aspiração folicular por videolaparoscopia e maturação oocitária in vitro em pacas (Cuniculus paca – linnaeus, 1766) mantidas em cativeiro e submetidas a protocolos de superestimulação ovariana

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Recolhimento mecanizado do café em função do manejo do solo e da declividade do terreno

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Impacto da maturação esquelética em biomarcadores do metabolismo ósseo e na densidade mineral óssea em adolescentes brasileiros saudáveis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Principais mecanismos envolvidos na maturação oocitária em bovinos: da oogenese a maturação in vitro

Despite the efforts made to improve the production of bovine embryos in vitro, their efficiency is still low, since only 30-40% of developed blastocysts are obtained from oocytes after in vitro maturation (IVM), fertilization and cultured embryos. Assisted reproductive technologies have a limiting impact due a lack of oocytes capable to fertilization.The comprehension of mechanism involved in oocyte maturation are crucial to establish a culture system that allows a larger number production of good quality embryos. The study of the early stages of oocyte and follicle development in vivo is important for a better understanding of the molecular pathways that regulate oogenesis, folliculogenesis and oocyte maturation. Thus the physiological biochemical and molecular mechanisms involved in maturation may contribute to the increased efficiency of in vitro embryo production. Therefore, the aim of this literature review is to understand the basic mechanisms that underlie oocyte maturation in cattle, since oocyte and follicle cells in vivo formation to its use in the in vitro environment.

Efeito do fator de crescimento epidermal (EGF) na maturação in vitro de oócitos caninos

Fundação de Apoio à Pesquisa do Estado de São Paulo (FAPESP)

Eficiência econômica no sistema de café orgânico: estudo de caso dos cooperados da COOPFAM

Aimed at satisfying a market niche, organic coffee production is a competitiveness-oriented system because it has less input costs and higher income. This study estimates the system’s economic efficiency, using production costs end gross income as economic input and output. This model was designed from original survey data, reported by a sample of producers in different stages of production, which enabled the development of average technical coefficients. Results show that annual gross income exceeds operating costs by 21%, pointing therefore to an economically efficient system.

Alterações na composição química e sensorial de café (Coffea arabica L.) nos processos pós-colheita

An interdisciplinary study was conducted to evaluate the effects of drying and storage time on changes in the quality of natural and fully washed coffees beans dried out in the yard and mechanically dried at a temperature of 60/40°C in air dryer machine. The coffee beans (Coffea arabica L.) harvested in cherries were processed by dry and wet methods, being subjected to pre-drying yard, followed by drying yard in the sun with air heated of 60/40°C until it reached the water content of 11% (wb). After reached the thermal equilibrium with the environment, the beans were packed in jute bag with a capacity of five kilograms and stored in uncontrolled environment during the period of one year, and removing material from each treatment every three months. To characterize the effect of drying and storage time on the coffee quality different methodologies was evaluated. It was observed less drying time for the fully washed coffee 60/40°C, and thus less energy consumed in the drying process until the point of storage, for the natural coffee there was significant effect of time on the chemical quality, biochemical and sensory; fully washed coffee proved to be more tolerant to drying than natural coffee, regardless of drying method, showing a better drink quality and less variation in chemical composition and biochemistry.