A high-fat diet increases interleukin-3 and granulocyte colony-stimulating factor production by bone marrow cells and triggers bone marrow hyperplasia and neutrophilia in wistar rats

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Intraocular pressure, specular microscopy, and prostaglandin E-2 concentration in dogs with mature and hypermature cataract

This study aimed to evaluate and correlate intraocular pressure (IOP), endothelial cell density (CD), and hexagonality (HEX), and the aqueous humor prostaglandin E-2 (PGE(2)) concentration in dogs with mature (MG, n = 8) and hypermature (HG, n = 8) cataracts. Eight laboratory beagles with no ocular abnormalities were included as a control group (CG). The IOP was measured using a digital applanation tonometer. Noncontact specular microscopy was used to evaluate CD and HEX. Samples of aqueous humor were used to determine prostaglandin E-2 concentration using enzyme-linked immunoassay. Data were compared by ANOVA and Bonferroni's multiple comparison test, and possible correlations among the PGE(2) aqueous concentration and corneal endothelium cell parameters were assessed by Person's test (P < 0.05). Average values of IOP (P = 0.45) and CD (P = 0.39) were not significantly different between MG, HM, and CG. Average values of HEX were lower, and PGE(2) concentration was increased in the MG and HG in comparison with CG (P < 0.05); however, such parameters did not change significantly between MG and HG (P > 0.05). PGE(2) values did not correlate with IOP, CD, and HEX in any group (P > 0.05). Although there were a small number of dogs studied, our results demonstrated that cataract progression from mature to hypermature did not have a significant change in PGE(2) aqueous concentration, IOP, corneal endothelial cell count, or morphology. In addition, PGE(2) concentration was not correlated with parameters of the corneal endothelium or IOP in dogs with mature or hypermature cataracts.

An��lise comparativa da produ����o e composi����o f��sico-qu��mica do leite considerando a utiliza����o de diferentes volumosos na alimenta����o de vacas Girolando

P��s-gradua����o em Medicina Veterin��ria - FMVZ

Procalcitonina e interleicina-6 em crian��as com diagn��stico de sepse e choque s��ptico

Sepse �� causa comum de mortalidade e morbidade em unidades de tratamento intensivo, sendo atualmente entendida como a resposta sist��mica �� infec����o, resposta esta caracterizada por duas ou mais das seguintes manifesta����es: temperatura corporal > 38��C ou < 36��C; taquicardia; taquipn��ia; e altera����o da contagem de c��lulas brancas (leucocitose ou leucopenia) ou mais de 10% de formas imaturas. Entretanto, tem sido descrito que os elementos que caracterizam a resposta sist��mica s��o muito sens��veis, insuficientemente espec��ficos e n��o levam em considera����o o avan��o do entendimento da fisiopatologia da doen��a. Al��m disso, a sepse tem fisiopatologia complexa, o que dificulta ainda mais o diagn��stico, principalmente em crian��as. O diagn��stico r��pido e a institui����o de tratamento o mais cedo poss��vel s��o imperativos nos pacientes s��pticos. Consequentemente, v��rios marcadores laboratoriais t��m sido estudados, sendo que dos v��rios mediadores envolvidos na sepse, procalcitonina (PCT) e interleucina-6 (IL-6) t��m despertado interesse pela possibilidade de auxiliar no diagn��stico e no estabelecimento da gravidade de pacientes adultos com sepse ou choque s��ptico, sendo que a PCT j�� foi inclu��da entre os crit��rios diagn��sticos de sepse nestes indiv��duos. Em pediatria... (Resumo completo, clicar acesso eletr��nico abaixo)

Efeitos do citrato de sildenafila sobre a neuroprote����o na neuropatia ��ptica, em ratos (Lewis/SsNHsd) com glaucoma agudo

P��s-gradua����o em Cirurgia Veterin��ria - FCAV

Hematological and biochemical changes in a Phrynops geoffroanus (Schweigger, 1812) population in response to environmental stressors

Turtles are among the most endangered vertebrate groups, and the main threats to populations are environmental pollution and habitat degradation. The species Phrynops geoffroanus, popularly known as ���Geoffroy���s side-necked turtle���, has proliferated in polluted environments, where adverse conditions could influence their living habits and physiological condition. Studies that monitor the effects of environmental pollution are key to understanding the species��� biology and designing effective conservation strategies. Thus, the analysis of hematological and biochemical parameters has been shown to be important in assessing the health of wild animals and risks for the animal and ecosystem. This study aimed to assess the environmental influence on the physiology of a P. geoffroanus population through the evaluation of antioxidant status and responses to environmental stressors, compared to specimens from a place under controlled conditions. Blood samples of 60 specimens were collected, 30 from the Felicidade Stream, polluted environment, within the city of S��o Jos�� do Rio Preto, and 30 from the ���Reginaldo Uvo Leone��� breeding farm, Tabapu��, SP, a place under controlled conditions, whose samples constituted the control group. They were evaluated by hemogram and by determining thiobarbituric acid reactive species (TBARS), Trolox-equivalent antioxidant capacity (TEAC) and the activities of the antioxidant enzymes catalase and glucose-6-phosphate dehydrogenase (G6PDH). There was a wide variation in hematological parameters of P. geoffroanus from the urban environment. The red blood cell count and hemoglobin values were significantly less than those observed in animals from the breeding farm (P = 0.0004; P = 0.0371, respectively). There was a significant increase in the number of thrombocytes (P < 0.0001) and leukocytes (P < 0.0001) in the animals from Felicidade Stream. The stress indices were similar between the two groups (P = 0.4077). TBARS levels showed the cytotoxic potential of compounds in the urban environment, whose animals had elevated levels of lipid peroxidation (P < 0.0001), despite showing a response to environmental damages with increase in antioxidant capacity, as demonstrated by the TEAC assay (P = 0.0207). The lower catalase enzyme activity noted in individuals from the urban environment (P = 0.000184) could be due to the presence of inhibitory compounds. On the other hand, G6PDH activity was higher (P = 0.002962), where this enzyme acts in the generation of NADPH, which is used in several detoxification pathways. We conclude that environmental contamination can increase oxidative damages and generate physiological changes in this species. These data are very useful for the conservation of P. geoffroanus and turtles in general, and confirm that these techniques are effective in monitoring natural regions and that P. geoffroanus can serve as an environmental contamination bioindicator.

Produ����o e qualidade do leite e da carne de caprinos anglo nubianos suplementados com ��leos vegetais

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Aspectos socioecon��micos, indicadores de qualidade do leite e pesquisa de linhagens toxig��nicas de Staphylococcus em propriedades de agricultura familiar de Botucatu, SP

P��s-gradua����o em Medicina Veterin��ria - FMVZ

Avalia����o dos resultados das contagens de c��lulas som��ticas obtidas pelo equipamento Ekomilk Scan e suas correla����es com outros m��todos de an��lise

P��s-gradua����o em Medicina Veterin��ria - FCAV

Avalia����o colostral de cabras acometidas por mastite e sua influ~encia nos n��veis imunes s��ricos de cabrito: Jefferson Filgueira Alcindo. -

P��s-gradua����o em Ci��ncia Animal - FMVA

Immunohistochemical evaluation of vascular endothelial growth factor (VEGF) in splenic hemangiomas and hemangiosarcomas in dogs

Formation of new blood vessels is paramount for tumour growth and metastatic dissemination and vascular endothelial growth factor (VEGF) is one of the key regulators of this process. The purpose of this study was to evaluate the immunohistochemical expression of VEGF in 23 splenic hemangiosarcomas and 7 splenic hemangiomas in dogs. Blood tests performed previous to splenectomy were analysed for correlation with tumour VEGF expression. Results showed significantly higher VEGF expression in hemangiosarcomas than hemangiomas and lower hematocrit values and red cell count in dogs affected with malignant neoplasia (P < 0.05). These findings suggest the presence of high VEGF levels may be related to the malignant vascular proliferation seen in hemangiosarcomas.

Esplenomegalia em c��es: estudo retrospectivo

The aim of this study was to review the prevalence of canine splenic disorders by means of a retrospectiveanalysis of histological diagnosis after splenectomy. The results were confronted with laboratory findings, clinical signs and presence of cardiac arrhythmia and hemoperitoneum, in an attempt to establish the differentialdiagnosis of splenomegaly. One hundred and nine dogs assisted at the Veterinary Hospital of theSchool of Veterinary Medicine and Zootechny, University of S��o Paulo, were included in the retrospectivestudy, between 2002 and 2009. The average age was 10 years old �� 3, with no sexual predilection. Mixedbreed dogs was the most affected kind, and average weight was 22 kg �� 13. Overall, 52% (57/109) of dogswere splenectomized due to non-neoplastic diseases and 48% (52/109) to neoplastic diseases. Among thelatter, the most common diagnosis was hemangiosarcoma (28 dogs; 54%). Most frequent clinical signs includedanorexia, lethargy and vomiting. Results showed that dogs with low red blood cell count, low hematocritvalues and/or hemoperitoneum were more likely to have splenic malignant neoplasm. On the otherhand, sex, breed, size, age, cardiac arrhythmia and other laboratory parameters were not determining factorsin the differentiation of splenomegaly.

Aditivos alternativos aos antibi��ticos promotores de crescimento no desempenho de frangos de corte sob desafio sanit��rio

P��s-gradua����o em Zootecnia - FCAV

Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Toxicidade do cloridrato de doxorrubicina na dose de 120mg/m��, em cadelas com tumor ven��reo transmiss��vel

P��s-gradua����o em Cirurgia Veterin��ria - FCAV