Calcium hydroxide root canal sealers - Histopathologic evaluation of apical and periapical repair after endodontic treatment

Eighty root canals of the premolars of 4 dogs, with vital pulp, were instrumented and filled during the same session with the Sealapex, CRCS, Sealer 26, and Apexit sealers, the animals were sacrificed 180 days after root canal filling and their maxillae and mandibles were removed and fixed in 10% formalin, After routine histologic processing, the sections were stained with hematoxylin-eosin and Mallory trichrome, Histopathologic analysis showed that Sealapex was the sealer that best permitted the deposition of mineralized tissue at the apical level and was the only sealer that provided complete sealing (37.5% of cases), With the use of Sealapex, no inflammatory infiltrate occurred and there was no reabsorption of mineralized tissues, In contrast, partial sealing and a moderate inflammatory infiltrate occurred with the use of CRCS, When Apexit and Sealer 26 were used the absence of sealing was frequent and active reabsorption of mineralized tissues occurred in most cases, the inflammatory infiltrate predominating with the use of Apexit was of the severe type, whereas with the use of Sealer 26 the inflammatory infiltrate was mild or absent.

Distribution and systematics of the freshwater genus Sirodotia (Batrachospermales, Rhodophyta) in North America

Multivariate morphometrics and image analysis were used to determine the number of well-delineated infrageneric taxa of Sirodotia in North America. Three groupings were distinguished from 25 populations examined from Newfoundland and Quebec in the north to central Mexico in the south. These groupings were statistically related to 10 type specimens, and the following species were recognized: Sirodotia huillensis (Welwitsch ex W. et G. S. West) Skuja (syn. S. ateleia Skuja), S. suecica Kylin (syn. S. acuminata Skuja ex Flint and S. fennica Skuja), and S. tenuissima (Collins) Skuja ex Flint. These species are differentiated on the basis of whorl shape and degree of separation at maturity (S. suecica, rounded and appressed; S. huillensis and S. tenuissima, truncated apex and separated), the density of spermatangia (S. huillensis, dense clusters, S. suecica and S. tenuissima, sparsely aggregated), and the mode of germination of the gonimoblast initial (S. suecica and S. tenuissima,from the nonprotuberant side of the fertilized carpogonium; S. huillensis from the protuberant side). Sirodotia huillensis was found only in the desert-chaparral, whereas S. suecica and S. tenuissima occurred from south-temperate to boreal regions in cool (temperature 8-18-degrees-C), low ion (specific conductance 10-99 muS.cm-1), and mildly acidic to neutral (pH 5.7-7.3) waters.

Zirconia-based dental crown to support a removable partial denture: a three-dimensional finite element analysis using contact elements and micro-CT data

Veneer fracture is the most common complication in zirconia-based restorations. The aim of this study was to evaluate the mechanical behavior of a zirconia-based crown in a lower canine tooth supporting removable partial denture (RPD) prosthesis, varying the bond quality of the veneer/coping interface. Microtomography (μCT) data of an extracted left lower canine were used to build the finite element model (M) varying the core material (gold core - MAu; zirconia core - MZi) and the quality of the veneer/core interface (complete bonded - MZi; incomplete bonded - MZi-NL). The incomplete bonding condition was only applied for zirconia coping by using contact elements (Target/Contact) with 0.3 frictional coefficients. Stress fields were obtained using Ansys Workbench 10.0. The loading condition (L = 1 N) was vertically applied at the base of the RPD prosthesis metallic support towards the dental apex. Maximum principal (σmax) and von Mises equivalent (σvM) stresses were obtained. The σmax (MPa) for the bonded condition was similar between gold and zirconia cores (MAu, 0.42; MZi, 0.40). The incomplete bonded condition (MZi-NL) raised σmax in the veneer up to 800% (3.23 MPa) in contrast to the bonded condition. The peak of σvM increased up to 270% in the MZi-NL. The incomplete bond condition increasing the stress in the veneer/zirconia interface.

Immunolocalization of aquaporins 1 and 9 in the ram efferent ducts and epididymis

Aquaporins (AQPs) are essential membrane protein channels for the transport of water across membranes. Fluid movement in the epididymis is important for modulation of the luminal environment, in which sperm mature and reside. This study was designed to understand the morphology and localization of AQPs in ram efferent ducts (ED) and epididymis. For this purpose, the epididymis of seven animals were removed for histologic and immunohistochemical analyses. AQP1 immunoreactivity was observed in the apex of the ED, and AQP9 was found adjacent to the nuclei of the epithelial cells of the ED. The epithelial lining of ram epididymis is pseudostratified columnar and presents principal, basal, apical and narrow cells. In the initial segment (IS), a moderate reaction for AQP1 was observed in the apical cytoplasm of epithelial cells. An intense reactivity for AQP1 was noted over the microvilli of principal cells and in spermatozoa in the caput. In the corpus and cauda, AQP1 was noted only over the endothelial cells of vascular channels located in intertubular spaces. A weak-to-moderate reaction for AQP9 was observed in the nuclei of epithelial cells in the IS, caput and corpus of the epididymis. In the cauda, an intense reaction to AQP9 was observed in the epithelial border. In the IS, caput and corpus, the reactivity for AQP9 differed from those observed in domestic animals. The cauda showed a pattern similar to that previously described. These results indicate that AQPs 1 and 9 have reversed locations and roles in rams, suggesting activity variations related with fluid and solute absorption throughout the epididymis.

Estudo in vitro da efetividade do hidróxido de cálcio e da polimixina B na eliminação das endotoxinas presentes nos canais radiculares

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação da perda óssea alveolar em pacientes encaminhados à especialista em periodontia: estudo radiográfico

The aim of this study was to evaluate the mean of alveolar bone loss (%) in radiographies of patients referred to specialist for periodontal treatment. Full mouth series of periapical radiographies (paralelism technique) were used. A total of 9808 proximal surfaces in 4804 teeth of 213 patients (90 males and 123 females), mean age, 44.3 years, were assessed. The alveolar bone loss was measured in relation to root lenght expressed as an average similar to Schei 's technique (1959) without the use of ruler. The references used were the distances cement-enamel juntion (CEJ) - alveolar crest (AC) and CEJ- dental apex taken with a digimatic caliper. The results showed that the mean values of alveolar bone loss (%ABL) was 22.39%. Men showed higher mean values of %ABL than women; the %ABL increased with the age, but the difference among age groups was no statistical significant (p>0.05); the mean values of %ABL of distal surfaces was higher than mesial surfaces (p<0.01), ríght quadrants exhibited higher %ABL values than left quadrants (p<0.01); maxillary sites showed mean values of %ABL higher than mandibular sites; the highest mean values of %ABL was found in molar group, followed by incisor groups, and premolar group; the canine group exhibited lower mean values of %ABL; the differences among the groups was considered statitistically significant (p<0.01). lt was suggested the usage of this technique in the dentistry offices and radiological services for the patient 's documentation and assortment and for later comparison about alveolar bone level

Influence of morphological variables in photoelastic models with implants submitted to axial loading

Purpose: This study used 12 photoelastics models with different height and thickness to evaluate if the axial loading of 100N on implants changes the morphology of the photoelastic reflection. Methods: For the photoelastic analysis, the models were placed in a reflection polariscope for observation of the isochromatic fringes patterns. The formation of these fringes resulted from an axial load of 100N applied to the midpoint of the healing abutment attached to the implant with 10.0mm x 3.75mm (Conexão, Sistemas de Próteses, Brazil). The tension in each photoelastic model was monitored, photographed and observed using the software Phothoshop 7.0. For qualitative analysis, the area under the implant apex was measured including the green band of the second order fringe of each model using the software Image Tool. After comparison of the areas, the performance generated by each specimen was defined regarding the axial loading. Results: There were alterations in area with different height and thickness of the photoelastic models. It was observed that the group III (30mm in height) presented the smallest area. Conclusion: There was variation in the size of the areas analyzed for different height and thickness of the models and the morphology of the replica may directly influence the result in researches with photoelastic models.

Cirurgias do Periápice

Apical surgeries are part of a segment of root canal treatment by which we can correct or eliminate iatrogenic deficiencies arising from conventional endodontic treatment. Currently no consensus among authors regarding to the factors linked to the surgery success as the execution of an apex resection followed by retropreparation and retrofilling. The apex resection performed 3mm from apical vertex allows the elimination of most apical ramifications, and it is a consensus part of the treatment. The preparation of cavities using ultrasonic tips has advantages over those made with drills such as better cleaning of the cavity, the possibility of lower cutting angle of the apical resection and deeper retropreparation. Another detail to consider is the type of tip, since the diamond tips in a more angulated inclination have higher cutting efficiency but causing increased formation of debris on the dentin walls. Finally, filling the cavity with an apical filling material allows proper sealing of the intracanal contents favoring apical healing.

Descrição de uma nova espécie de Rhodnius (Hemiptera, Reduviidae, Triatominae) da Amazônia

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Sensitivity of yellow passion fruit plants to ultraviolet-B radiation

The objective of this work was to evaluate the effects of UV-B radiation on the vegetative growth and on the gas exchange characteristics of passion fruit plants (Passiflora edulis) grown in greenhouse. The average unweighted UV-B radiation near the apex of the plants was 8 W m-2 for the UV-B treatment (high UV-B), and 0.8 W m-2 for the control plants (low UV-B). Plants were irradiated with UV-B for 7 hours per day, centered on solar noon, during 16 days. High UV-B radiation resulted in lower shoot dry matter accumulation per plant. The content of UV-B absorbing compounds and anthocyanins was increased in the plants exposed to high UV-B radiation, when compared with the control. UV-B radiation did not affect stomatal conductance or transpiration rate, but reduced photosynthesis and instantaneous water‑use efficiency, and increased intercellular CO2 concentration. The accumulation of UV-B-absorbing compounds and anthocyanins did not effectively shield plants from supplementary UV-B radiation, since the growth and photosynthetic processes were significantly reduced.

A aplicação da abordagem do Tecnógeno na identificação e classificação dos terrenos e depósitos tecnogênicos no ambiente urbano de Guarapuava-PR

Pós-graduação em Geografia - FCT

Efeitos de formulações e intervalos sem chuva na absorção, translocação e eficácia de Glyphosate e 2,4-D

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Poda apical para produção de frutos e sementes de abóbora

The tip pruning stimulates the emission of lateral shoots, thus can produce higher number of flowers, fruits and seeds. This work aimed to evaluate the effect of apex pruning on fruit and seed production in pumpkin. The treatments consisted of plants without pruning, with pruning in the sixth, eighth and tenth node of main stem. The experimental design was a randomized block, with six replications. It was studied a line of pumpkin of the Germplasm Bank of the Universidade Estadual Paulista/Faculdade de Ciências Agronômicas. The characteristics evaluated were: number of branches per plant and fruit position in branches of the plant production (number and weight) of fruits per plant, fruit yield, fruit average weight, fruit length and diameter, seed weight per fruit, seed yield and quality (germination test, first count, thousand seed weight, emergence, speed of emergence and accelerated aging). There was a significant difference only for number of secondary branches per plant and thousand seed weight, with larger values for plants that have not been pruned. It was obtained high average of germination (94%) and good fruit (16.9 t ha-1) and seed (148 kg ha-1) yield. The apex pruning does not influence the production of fruits and seeds, as well as the physiological seed quality in pumpkin.

Interpretação racional do ensaio DPL

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Diferenciação de laticíferos articulados em plantas de Tabernaemontana catharinensis (Apocynaceae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)