Culicídeos vetores em uma unidade de conservação da caatinga na região do seridó no Rio Grande do Norte aspectos da transmissão de doenças

The scarcity of occurrence records of culicid species, transmitting insects of etiologic agents for arboviruses, protozoan diseases and philarioses, and lack of information about their interactions with the forest environment make the Caatinga one of the most unknown biomes in Brazil. The aim of this study was to identify culicid species in a conservation unit located in a preserved area of the Caatinga and the possible associations with local abiotic factors, as well as characterize the natural and artificial ecotopes present in the Caatinga of Rio Grande do Norte state. The Ecological Station of Serido (ESEC-Seridó) is a Caatinga Conservation Unit located in a rural area of Rio Grande do Norte. The hot and dry climate of the Caatinga, along with a short rainy season, favor the adaptation and resistance of plants, resulting in completely distinct landscapes over the course of the year. The study took place over the course of one year, with monthly collections between 10h-12h, 14h-16h and 18h-20h. Adult mosquitoes were collected in a forest area using a manual suction trap with human bait as attractant at the three times and a Shannon trap for night collections. During the rainy season ovitraps were randomly distributed at different sites. The different capture sites were observed and photographed. Immature and adult insects captured were taken to the laboratory for identification. Of the 5081 insects collected in human-bait and Shannon traps, 75% were Mansonia wilsoni. In addition, Aedeomyia, Aedes, Anopheles, Coquillettidia, Culex, Haemagogus, Mansonia and Psorophora were identified. Of the 92 Haemagogus eggs found, 71 were collected on the banks of a reservoir. Mosquito larvae were found and photographed in an artificial breeding site of a neighboring rural area. Humidity had the greatest influence on culicid density in the Caatinga. Rain was important in the appearance of Haemagogus, Anopheles, Aedes and Psorophora. Temperature had a negative influence on Anopheles triannulatus. The occurence of important vector species of diseases in a little known preserved area of the Caatinga as well as environmental and sociocultural questions are factors that favor the emergence of transmitting insects, making these areas potential subject to the resurgence of diseases

Diretrizes para conservação da espécie Mimosa Caesalpiniifolia Benth., Macaíba-RN

About 40% of the earth is occupied by tropical and subtropical forests, including 42% of dry forests, where there is Caatinga Bioma, contemplating tree forests and shrubs, with xerophytic characteristics. Study and conservations of Caatinga biologic diversity is one of the greatest challenges of Brazilian science because those are, proportionally, the less studied among natural areas, with most of the scientific effort centered in very few points around the main cities in the area and also because it is the less protected natural Brazilian area. The environmental degradation is constantly increasing and has its rhythm accelerated by the men appropriation to meet or not their own needs. Therefore, species conservation should be based in three principles: the use of natural resources by present generation, waste prevention and use of the natural resources to benefit the majority of the citizens. Among the strategies to species conservation, we can mention the ex situ conservation , in which the conservation of genetic resources may be realized outside of the natural environment in which the species occur, and in situ conservation , or, in other words, in the places where the species occur. In ex situ conservation, the germplasm collections are maintained in the field and/or in laboratories (conservation chambers), and this mainly conserves intraspecific diversity (genetic variance), the ex situ collections are continuously enriched by collection activities, introduction and germplasm interchange; the in situ conservation preserving ecosystems and habitats, maintaining and recovering native population of species of interest. So, the objective of this paper is the search for strategies to the conservation of Mimosa caesalpiniifolia B. (sabiá) using instruments of environmental perception and plant biotechnology, as mechanisms of in situ and ex situ conservation. To environmental perception, were realized open, semi-structured and qualitative interviews. The questions included socioeconomic data and knowledge of Sabiá specie. To plant biotechnology, Sabiá seed collection were realized in different location to formation of a germplasm bank. The specie micropropagation was made from nodal segment of plants from the matrizeiro. About the knowledge of rural populations and the use of Sabiá plant, some preferences occurred from speeches that the plant possesses a firm wood, not attacked by termites, legalized for exploration by the Brazilian environmental organ (IBAMA), and is a native specie. This research found the rural population has knowledge about Sabiá specie and the natural resources are exhausting. The proposal that the rural community brought was the donation of the Sabiá specie seeding initiating on the rain season, in which the seeding would be plated between the lots, in individual plantations. To the formation of a matrix bank, plant biothecnology brought answers favorable to Sabiá specie seeding, with the formation of multiple shoots

Conservação dos invertebrados em área de proteção ambiental litorânea no nordeste do Brasil: contribuições da educação ambiental para a sustentabilidade

Today's society is in a real collapse of an environmental nature. The populations are lost due to a dream of development without thinking of the consequences that said development can bring to human populations. Thus, it is necessary that strategies be developed for the purpose of protecting the flora and fauna that is at risk from suffering the pressure to achieve this development. Thus important issues involving the need to conserve nature and the creation of protected areas as these strategies are increasingly being developed in research, whether in the biological and / or social. In this sense, the aim of this research through environmental perception social actors for the formation of significant elements for understanding the relationship between man and nature, and from there to provide actions for sensitization. As well as changing attitudes towards environmental issues, to thereby provide analysis based on Environmental Education in order to provide the production of environmental knowledge as a tool that provides value shift. This area of research was to study the Environmental Protection Area Jenipabu - APA Jenipabu, located in northeastern Brazil. Where, from the environmental perception of students from schools within and around this Nature Conservation Unit notes were made regarding the value and meaning given by students, and how this, the feeling of belonging to these groups. This dissertation is composed of two chapters, the first is titled Environmental perception and feeling of belonging in the area of environmental protection in coastal RN - Brazil, where it makes a diagnosis of how these groups understands and realizes the Unity of Nature Conservation. The second, which is titled Construction of environmental knowledge and conservation of invertebrates in the Environmental Protection Area in the northeast coast of Brazil, specifically developed in the school from within the APA Jenipabu, in order to promote a sense of belonging for those students who become multipliers, in order to realize the importance and necessity of having this unit for Nature Conservation. Looking to the degree of importance of environmental education as a tool to raise awareness on conservation of invertebrates and is all the fauna and flora exists, whether in a conservation of nature or not

Conservação de espécies nativas em um assentamento rural do município de Apodi-RN: impactos socioambientais e econômicos e o uso da biotecnologia como alternativa promissora

The Caatinga biome has a high diversity of potential and their conservation constitutes one of the greatest challenges of Brazilian science. The sustainable management of the Caatinga emerges as an alternative that through the formation of systems agrossilvipastoris, enables the use of forest resources sustainably, ensuring their conservation, regeneration and recovery. In RN this technique has been developed mainly in settlements of Agrarian Reform, such as P. A. Moaci Lucena, and their impacts go beyond the environmental aspect and reverberate socially and economically on the quality of life of family farmers. Despite the efficiency of the Sustainable Management of the Caatinga in the conservation of native species, many forests species of this biome faces serious problems of propagation and for this reason have become vulnerable to extinction, as is the case of Mimosa caesalpiniifolia Benth . Thus , it is evident the need to use sustainable alternatives to overcome the difficulties of propagation of this species and enable their replacement in areas where their existence is threatened. The Plant Biotechnology is considered as a promising alternative in this sense, considering that by micropropagation enables the large-scale production of seedlings with high health genetics status. This work has the following objectives: evaluate the perception of family farmers of P. A. Moaci Lucena in relation to social, environmental and economic impacts of the Sustainable Management of the Caatinga and check the conditions of germination and in vitro propagation of Mimosa caesalpiniifolia Benth that enabling the production of seedlings of this specie on a large scale. To achieve the first objective, semi-structured interviews showed that in the perception of farmers PA Moaci Lucena, the Sustainable Management of the Caatinga was responsible for generating many social, environmental and economic impacts that affected directly in the improvement in the quality of life of the families of the Settlement Project Moaci Lucena. Have to achieve the second objective, were investigated the influence of different substrates and concentrations of growth regulator BAP in the germination and shoot induction in vitro of Mimosa caesalpiniifolia Benth. The vermiculite was presented as the most suitable substrate for germination of this species, because it provided a more rapid germination, higher growth rates and higher dry matter accumulation. Regarding micropropagation, the concentration of 17.76 μmol/L of BAP presented a more responsive in relation to multiplication rate and the number of shoots in M. caesalpiniifolia, thus constituting the most suitable concentration for the in vitro propagation of this specie

Agroindústria canavieira e unidade de conservação: Impactos Sociais na comunidade de pescadores de Baía Formosa (RN)

The conservation unities emerged in response to the environmental damages. In the Atlantic Forest of the Brazilian Northeast, the most of the damages come from the sugar-cane agro-industry, especially by the deforestation for the introduction of sugar-cane fields and installation of the industry structure. Besides the damages over the biodiversity, there are critical social problems that affect the communities which survive using directly or indirectly the biome from the Atlantic Forest. The objective of this research is to analyze the social impacts on the fishermen of Baía Formosa/RN generated by the changes and socio-environmental transformations occurred starting the installation of a sugar-cane factory and the creation of a conservation area in the municipality of Baía Formosa/RN community. The research is based on a qualitative and diachronic perspective, for both approach, it was used the method of oral history in conjunction with the literature search and some technical and research tools such as direct observation, interview, records images and recordings . The results show that social and symbolic practices of the community in the conservation unit Mata Estrela last more strongly presented. Today fewer natural resources are used, due to factors such as limit access to forest and disappearance of some natural resources by deforestation. It is believed that the loss of free access to space for the community is the most significant negative impact, as promoted changes in the relationship of the community with the Star Forest that contributed to the fading of their social and symbolic practices. The loss of free access caused the decline in the practice of agriculture, which resulted in the loss of one of the means of social reproduction of the community

Proposta de critérios adicionais para definição de Área Prioritária para Conservação no Semiárido brasileiro

Environmental changes and their consequences on the global level have challenged the different fields of study to integrate towards effective solutions to minimize and /or equate the negative impacts of these changes in different types of environments. In this context, the Environmental Perception has been a widely used and relevant in studies that consider the relationship between the environment and human actions, by allowing analysis of perceptions, attitudes and values, key influencers of topophilia that resonates in conservation tool. Allied to Environmental Perception, Integrated Analysis of the Landscape is relevant because it allows to analyze in a systematic way the geographical space where all its elements are interrelated in a way that supports needed to understand the complex physical and human environment of a given environment. In this perspective, we have studied the João do Vale Serrano Complex, located in semiarid of Rio Grande do Norte state, which features a set of landscapes with different faces, which are being replaced by various economic activities and disordered population growth, with consequent exploitation the potential of natural resources. This thesis main goal was to combine the Environmental Perception of rural communities to the of Serrano Complex Landscape Analysis as additional criteria for the definition of Priority Areas for Conservation. The perception data were collected through direct observation, questioning, interviews and application forms to 240 people (100 % of occupied households in the mountain community) during the months of february and august 2011, with theoretical and methodological basis Environmental Perception. Integrated Landscape Analysis was performed by GTP (Geosystem - Territory - Landscape) method, using the Geographic Information System (GIS), using the technique of GIS for mapping the landscape. The results showed that respondents have a sense of topophilia by where they live, hold a vast knowledge of the natural resources in this Serrano Complex, and responded positively regarding the choice of an exclusive area for conservation. The Integrated Analysis of Landscape possible to identify the different forms of existing uses and occupations in Serrano Complex, have caused significant changes in space, especially on the plateau where vegetation was virtually replaced by human dwellings and cashew plantations. Through the maps of slope and environmental vulnerability was identified that areas with high slopes (gullies) are limiting factor for occupation by communities and therefore relevant and amenable to conservation, including by being Permanent Preservation Areas. These results, together, made possible to define a map of Priority Areas for Conservation in Serrano Complex, with three priority categories: low, medium and high. Therefore, the use of these additional criteria are relevant for the definition /designation of Priority Areas for Conservation

Pós-colheita de pêssegos (Prunus pérsica L. Bastsch) revestidos com filmes a base de amido como alternativa à cera comercial

O presente trabalho objetivou prolongar a conservação póscolheita de pêssegos, armazenando-os à temperatura ambiente. Inicialmente selecionou-se uma microemulsão à base de fécula de mandioca e cera de abelha. Posteriormente ela foi testada, aplicando-a na superfície dos frutos em comparação com Fruit wax (cera comercial), com o intuito de se verificar o efeito dos diferentes tratamentos na composição química, física e físico-química dos mesmos. Utilizaram-se pêssegos 'Biuti' colhidos manualmente em 14/01/1999, ao atingirem o ponto de maturação fisiológica. do lote colhido foram selecionados 120 frutos sendo os mesmos analisados quanto a perda de massa fresca, taxa respiratória, textura, sólidos solúveis totais, acidez total titulável e pH, a cada 3 dias. Os frutos receberam os tratamentos: Testemunha, Fruit Wax, Fécula e Microemulsão. Os tratamentos Fruit Wax e Microemulsão proporcionaram melhor eficiência em relação à perda de massa fresca que os frutos dos tratamentos Testemunha e Fécula. Quanto à taxa de respiração, verificou-se picos da ordem de 40mg de CO2.kg-1.h-1 . Quanto aos açúcares, verificou-se que a sacarose foi o açúcar encontrado em maior quantidade, com apenas traços de glicose e frutose em algumas amostras. Quanto aos teores de sólidos solúveis totais, os frutos tratados com Fruit Wax apresentaram valores inferiores aos do tratamento Testemunha. O efeito da Microemulsão mostrou-se similar ao da cera Fruit Wax em todos os atributos e, superior ao dos tratamentos Testemunha e Fécula na redução da perda de massa fresca.

Leite cru de quatro regiões leiteiras brasileiras: perspectivas de atendimento dos requisitos microbiológicos estabelecidos pela Instrução Normativa 51

Evidências que o leite produzido e consumido no Brasil nem sempre apresenta a qualidade desejada têm gerado a discussão e desenvolvimento de novas políticas de incentivo à produção leiteira, resultando no desenvolvimento do Programa Nacional de Melhoria da Qualidade do Leite. em complementação, em 2002 o Ministério da Agricultura publicou a Instrução Normativa 51 (IN51), com importantes inovações em relação à conservação e transporte do leite cru, além de estabelecimento de um padrão de qualidade para esse tipo de leite (10(6) UFC/mL), a ser implantado em diferentes prazos nas diferentes regiões do país, a partir de 2005. O presente trabalho teve como objetivo verificar se o leite cru produzido em quatro áreas de quatro estados produtores de leite no Brasil estaria, nesse momento, em condições de cumprir o estabelecido na IN 51, especialmente quanto ao atendimento dos padrões microbiológicos previstos. Amostras de leite cru, coletadas em 210 diferentes propriedades nas regiões de Viçosa, MG (47), Pelotas, RS (50), Londrina, PR (63) e Botucatu, SP (50), foram analisadas quanto aos níveis de contaminação por aeróbios mesófilos, utilizando o PetrifilmTM AC. Parcela significativa das amostras (48,6%) apresentaram contagens acima do determinado pela IN51, sendo 21,3% na região de Viçosa (MG), 56,0% na região de Pelotas (RS), 47,6% na região de Londrina (PR) e 68,0% na região de Botucatu (SP). Considerando as diferenças de cada região, foi possível observar a importância da refrigeração na conservação e transporte da produção, bem como da implantação de boas práticas e assistência técnica nas propriedades. Os resultados obtidos permitem concluir que a adequação às normas estabelecidas pela IN51 pode ser mais difícil em algumas regiões do que em outras, sendo fundamental a adoção da refrigeração na conservação e no transporte da produção, e de programas regionais de assistência a produtores leiteiros.

Valor nutritivo dos alimentos utilizados na formulação de rações para peixes tropicais

A seleção de ingredientes para a formulação de dietas para peixes requer o conhecimento prévio dos coeficientes de digestibilidade aparente da energia e nutrientes. O valor nutritivo de vários alimentos tem sido investigado no Brasil e o uso de fontes alternativas não deve resultar em efeitos negativos sobre o desempenho, saúde dos peixes e qualidade da água. Diversos fatores podem afetar a digestibilidade, sendo que os coeficientes de digestibilidade aparente dos ingredientes devem ser utilizados para a formulação de rações mais precisas e econômicas.

Efeitos da suplementação de fitase sobre a disponibilidade aparente de Mg, Ca, Zn, Cu, Mn e Fe em alimentos vegetais para a tilápia-do-nilo

Cem juvenis de tilápia-do-nilo (Oreochromis niloticus; PV = 100.0 ± 5.0 g) foram distribuídos em 10 tanques-rede com o objetivo de avaliar o efeito da suplementação da enzima fitase (0, 1.000 e 2.000 UFA/kg) sobre a disponibilidade de minerais em alguns alimentos energéticos (milho, milho extrusado, farelo de trigo, farelo de arroz e farelo de sorgo) e protéicos (farelo de soja extrusado, farelo de soja, farelo de girassol, farelo de algodão e glúten de milho) utilizados na alimentação de tilápia-do-nilo (Oreochromis niloticus). Para determinação dos coeficientes de disponibilidade aparente (CDA) do cálcio (Ca), magnésio (Mg), zinco (Zn), cobre (Cu), ferro (Fe) e manganês (Mn), foram confeccionadas 31 rações, marcadas com 0,10% de óxido de crômio III uma referência (ração purificada) e 30 contendo os dez alimentos e os diferentes níveis de suplementação da enzima fitase. O CDA dos nutrientes foi calculado com base no teor de crômio da ração e das fezes. A fitase aumenta, nos vegetais, a disponibilidade do Mg, Cu, Zn e Mn, os quais apresentam tendência diferenciada, em razão do seu valor biológico e do nível de suplementação de enzima.

Disponibilidade aparente do fósforo em alimentos vegetais e suplementação da enzima fitase para tilápia-do-nilo

Este estudo foi realizado com o objetivo de avaliar o efeito da fitase (0, 1.000 e 2.000 uf/kg) na disponibilidade aparente de P de cinco alimentos energéticos (milho, milho extrusado, farelo de trigo, farelo de arroz e sorgo baixo tanino) e cinco alimentos protéicos (soja extrusada, farelo de soja, farelo de girassol, farelo de algodão e glúten de milho) utilizando-se uma ração purificada como controle. Foram utilizados 100 juvenis (100,00 ± 5,00 g) de tilápia-do-nilo (Oreochromis niloticus) alojados em dez gaiolas para manejo de alimentação e coleta de fezes. Para cada alimento e cada nível de fitase avaliado, foram coletadas amostras em cinco gaiolas, totalizando cinco repetições por tratamento. Os resultados obtidos indicaram que a suplementação de até 2.000 uf/kg de fitase não aumentou a disponibilidade aparente de P do farelo de trigo e do farelo de algodão. Entretanto, o nível de 1.000 uf/kg aumentou a disponibilidade aparente de P da soja extrusada e do farelo de girassol, enquanto o nível de 2.000 uf/kg proporcionou essa resposta para o farelo de milho, o milho extrusado, o sorgo baixo tanino, o farelo de arroz, o farelo de soja e o glúten de milho.

Digestibilidade de alimentos protéicos e energéticos para fêmeas de beta

Foram avaliados os coeficientes de digestibilidade aparente de MS, PB, energia bruta (EB) e EE de alimentos protéicos (farelo de soja e farinha de peixe) e energéticos (fubá de milho e farelo de trigo) para beta (Betta splendens). Fêmeas adultas foram alojadas em gaiolas e mantidas em dois aquários de fibra de vidro (30 peixes/aquário) para alimentação e dois para coleta de fezes, ambos de formato cônico e com capacidade para 30 L. Os resultados dos coeficientes de digestibilidade aparente de MS, PB, EB e EE foram, respectivamente, de 69,43; 72,52; 67,91 e 55,50% para farelo de soja; 60,67; 51,15; 75,55 e 58,26% para farinha de peixe; 63,88; 87,16; 77,61 e 50,40% para fubá de milho; e 61,06; 93,37; 58,17 e 65,51% para farelo de trigo. Os resultados obtidos neste estudo permitem otimizar a formulação de dietas práticas balanceadas, economicamente viáveis para a espécie.

Própolis e cera vegetal na conservação de abacate Hass

The aim of this work was to evaluate the quality of Hass avocado submitted to application of propolis and vegetable wax. The selected fruits were submitted to the treatments (1) without wax and without propolis, (2) with vegetable wax, (3) propolis alcoholic extract 100%, (4) wax and etanol 70%, (5) propolis alcoholic extract 2% and wax, (6) propolis alcoholic extract 4% and wax, (7) propolis alcoholic extract 6% and wax, (8) propolis alcoholic extract 8% and wax, and (9) propolis alcoholic extract 10% and wax. The fruits were maintained under refrigeration (10 +/- 1 degrees C and 90 +/- 5% relative humidity), being appraised every three days for 15 days and more 3 days of commercialization simulation at room temperature (23 +/- 1 degrees C and 18% relative humidity). The evaluation parameters were the weight loss, respiratory activity, pH, firmness, tritable acidity (AT), soluble solids (SS), and ratio. The results of weigth loss, respiratory activity and firmness were the most significant to show the difference among the treatments. 'Hass' avocado fruits submitted the application of propolis associated the vegetable wax present smaller weight loss, CO2 production and firmness in relation to the control fruits. The fruits of the treatment with propolis alcoholic extract 2% and wax presented the most promising results.

Uso de reguladores vegetais na conservação refrigerada de acerolas (Malpighia glabra L.)

Com o objetivo de avaliar o uso do ácido giberélico (GA3) e da benzilaminopurina (BAP) na conservação de acerolas (Malpighia glabra L.) colhidas no estádio verde e armazenadas sob refrigeração, acerolas foram submetidas aos seguintes tratamentos, sob imersão por 30 minutos: controle (água), 50 mg L-1 e 100 mg L-1 de GA3, 50 mg L-1 e 100 mg L-1 de BAP. Após os tratamentos, os frutos foram deixados para secar ao ar em local fresco e, então, embalados em bandejas de isopor cobertas com filme de polietileno e armazenados em câmara B.O.D a 8±1ºC, por 14 dias. As avaliações foram realizadas em intervalos de 4 dias. Os frutos amostrados foram submetidos a avaliações de coloração, teor de sólidos solúveis, acidez titulável e teor de ácido ascórbico. A análise dos resultados mostrou que a aplicação dos reguladores não teve efeito no aumento da conservação refrigerada de acerolas e que somente a refrigeração foi suficiente para conservá-las durante 14 dias.

Ocorrência e conservação de Touit melanonotus (Aves, Psittacidae) no sul do Brasil

O apuim-de-costas-pretas [Touit melanonotus (Wied, 1820)] é uma espécie florestal endêmica da Mata Atlântica Brasileira e de relevante interesse conservacionista. Trata-se de uma ave com poucas informações disponíveis acerca de sua história natural e distribuição geográfica e novas observações são importantes fontes de informação para auxiliar na conservação da espécie. Aqui reavaliamos o primeiro registro da espécie e apresentamos novas observações no estado do Paraná, sul do Brasil. As vocalizações atribuídas a T. melanonotus e que consubstanciaram sua ocorrência no estado foram examinadas com auxílio de espectrogramas e identificadas como vozes de Pionopsitta pileata (Scopoli, 1769). Não obstante, T. melanonotus ocorre com certa regularidade no Paraná, como indicado por novos registros da espécie em sete localidades distribuídas por todo o litoral do estado. Esta ave foi registrada principalmente em florestas de terras baixas e, uma vez que esta fisionomia sofre intensas pressões antrópicas, sugerimos que esforços de conservação da espécie devem priorizar a preservação desses hábitats.