Discurso pronunciado por el Excmo. Sr. D. Rafael Portaencasa Baeza, Rector Magnífico de la Universidad Politécnica de Madrid, con motivo de la apertura del curso académico 1992-93 e investidura de Doctor Honoris Causa de los Profesores Dres. Carlo Rubbia y James L. Flanagan

Discurso en el que el Rector D. Rafael Portaencasa inaugura oficialmente el comienzo del curso académico 1992-93 y repasa los principales méritos de los dos nuevos doctores honoris causa, investidos durante este acto académico.

La Traca : semanari pa la gent de tro: Año III Número 93 - 12 septiembre 1886

Recurso electrónico. Valencia: BVNP, 2014

Relative role of heme nitrosylation and β-cysteine 93 nitrosation in the transport and metabolism of nitric oxide by hemoglobin in the human circulation

To quantify the reactions of nitric oxide (NO) with hemoglobin under physiological conditions and to test models of NO transport on hemoglobin, we have developed an assay to measure NO–hemoglobin reaction products in normal volunteers, under basal conditions and during NO inhalation. NO inhalation markedly raised total nitrosylated hemoglobin levels, with a significant arterial–venous gradient, supporting a role for hemoglobin in the transport and delivery of NO. The predominant species accounting for this arterial–venous gradient is nitrosyl(heme)hemoglobin. NO breathing increases S-nitrosation of hemoglobin β-chain cysteine 93, however only to a fraction of the level of nitrosyl(heme)hemoglobin and without a detectable arterial–venous gradient. A strong correlation between methemoglobin and plasma nitrate formation was observed, suggesting that NO metabolism is a primary physiological cause of hemoglobin oxidation. Our results demonstrate that NO–heme reaction pathways predominate in vivo, NO binding to heme groups is a rapidly reversible process, and S-nitrosohemoglobin formation is probably not a primary transport mechanism for NO but may facilitate NO release from heme.