Carbon isotope composition as a tool to control the quality of herbs and medicinal plants

Isotope screening is a simple test for determining the photosynthetic pathway used by plants. The scope of this work was to classify the photosynthetic type of some herbs and medicinal plants through studies of the carbon isotope composition (δ13C). Also, we propose the use of carbon isotope composition as a tool to control the quality of herbs and medicinal plants. For studies of δ13C, δ 13C‰ = [R (sample)/R (standard) - 1] × 10-3, dry leaves powdered in cryogenic mill were analyzed in a mass spectrometer coupled with an elemental analyzer for determining the ratio R = 13CO2/12CO2. In investigation of δ13C of 55 species, 23 botanical families, and 44 species possessed a C3 photosynthetic type. Six species found among the botanical families Euphorbiaceae and Poaceae were C4 plants, and 5 species found among the botanical families Agavaceae, Euphorbiaceae, and Liliaceae possessed CAM-type photosynthesis. Carbon isotope composition of plants can be used as quality control of herbs and medicinal plants, allowing the identification of frauds or contaminations. Also, the information about the photosynthetic type found for these plants can help in introducing and cultivating exotic and wild herbs and medicinal plants.

Carbon isotope composition as a tool for quality control of herbs, spices and medicinal plants

Isotope screening is a simple and cheap test for determining the photosynthetic pathway used by plants. The scope of this work was to classify the photosynthetic type of Melissa officinalis L. and Cymbopogon citratus [DC.] Stapf, through studies of the carbon isotope composition (δ13 C), and we are proposing the use of carbon isotope composition results as a tool to control the quality of medicinal plants. For studies of δ 13C (13 C% = [R (sample)/R (standard) - 1] × 10 -3), dried, powdered leaves were analyzed in a mass spectrometer coupled with an elemental analyzer for determining the ratio R (R = 13CO2/12CO2). As results, M. officinalis presented a C3 photosynthetic type, and C. citratus presented a C4 photosynthetic type. The carbon isotope composition from this study can be used as quality control of M. officinalis adulterants.

Experimental study of a methodology for Fission-track Dating without neutron irradiation

To carry out the dating by the Fission Track Method (FTM) the international community that works with this method employs methodologies in which the mineral to be dated must be irradiated with neutrons. Such irradiation, performed in a nuclear reactor, demand a relatively long waiting time so that the activity of the sample attain a proper level for handling. The present work aims to establish a methodology that makes possible the dating by FTM using a mass spectrometer instead of a nuclear reactor. This methodology was applied to apatite samples from Durango, Mexico. © 2009 Elsevier Ltd. All rights reserved.

Indoor air quality assessment of elementary schools in Curitiba, Brazil

The promotion of good indoor air quality in schools is of particular public concern for two main reasons: (1) school-age children spend at least 30% of their time inside classrooms and (2) indoor air quality in urban areas is substantially influenced by the outdoor pollutants, exposing tenants to potentially toxic substances. Two schools in Curitiba, Brazil, were selected to characterize the gaseous compounds indoor and outdoor of the classrooms. The concentrations of benzene, toluene, ethylbenzene, and the isomers xylenes (BTEX); NO2; SO2; O3; acetic acid (HAc); and formic acid (HFor) were assessed using passive diffusion tubes. BTEX were analyzed by gas chromatography-ion trap mass spectrometry and other collected gasses by ion chromatography. The concentration of NO2 varied between 9.5 and 23 μg m-3, whereas SO2 showed an interval from 0.1 to 4.8 μg m-3. Within the schools, BTEX concentrations were predominant. Formic and acetic acids inside the classrooms revealed intermediate concentrations of 1.5 μg m-3 and 1.2 μg m-3, respectively. © Springer Science + Business Media B.V. 2009.

Análise isotópica (δ13c) e legalidade em néctares de uva

The aim of this work was to develop an isotopic analysis method to quantify the carbon of C3 photosynthesis cycle in grape nectar and to identify the commercial beverages in disagreement to the Brazilian Ministry of Agriculture, Livestock and Food Supply (MAPA) regulation. The nectars were produced in a laboratory, according to the Brazilian Law. Adulterated beverages with quantity of grape juice lower than the legal limit were also produced. Isotopic analysis measured the relative isotopic enrichment of grape nectar and its purified sugar fraction. Based on these results, it was possible to estimated the quantity of source C3 by means of isotopic dilution equation. To determine the existence of adulteration in commercial nectars, it was necessary to create a legal limit according to the Brazilian Law. One of the twelve commercial brands of nectar analyzed was classified as adulterated. The developed methodology proved to be efficient to quantify the carbon of C3 origin and identify the adulterated commercial grape nectar.

Alocação de fotoassimilados marcados e relação fonte-dreno em figueiras cv. Roxo de Valinhos. 1. Relação fonte e dreno

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Alocação de fotoassimilados marcados e relação fonte-dreno em figueiras cv. Roxo de Valinhos. 2. Tempo de alocação

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

ASTER: A Brazilian mission to an asteroid

The first Brazilian mission to an asteroid is being planned. The target is the asteroid 2001 SN263, which has a NEA orbit of class AMOR. Spectral analysis indicated that this is a C-type asteroid. This type of asteroids are dark and difficult to be studied from Earth. They hold clues of the initial stages of planetary formation and also the origin of water and life on Earth. In fact, radar data showed that 2001 SN263 is composed of three bodies with diameters of about 2.8 km, 1.1 km and 0.4 km. Therefore, the spacecraft will have the opportunity to explore three bodies on the same trip. The mission is scheduled to be launched in 2015, reaching the asteroid in 2018. It will be used a small spacecraft (150 kg) with 30 kg for the payload. The set of scientific instruments being considered to explore the target of this mission include an Imaging Camera, a Laser Rangefinder, an Infrared Spectrometer, a Synthetic Aperture Radar and a Mass Spectrometer. The main measurements to be made include the bulk properties (size, shape, mass, density, dynamics, spin state), the internal properties (structure, gravity field) and surface properties (mineralogy, morphology, elemental composition). The mission also opens an opportunity for some relevant experiments, not directly related to the target. Two such experiments will take benefit from being on board of the spacecraft along the journey to the asteroid system, which will take about three years. The first is an astrobiology experiment. The main goal of this experiment is to determine the viability of the microorganisms survival in extraterrestrial environments simulated in laboratory (chemical atmosphere, temperature, desiccation, vacuum, microgravity and radiation). The second experiment is a plasma package. The main objectives of this experiment are to study the structure and electrodynamics of plasma along the trajectory, the plasma instability processes and the density and temperature of plasma of solar wind origin along the trajectory and near the asteroids. This mission represents a great challenge for the Brazilian space program. It is being structured to allow the full engagement of the Brazilian universities and technological companies in all the necessary developments to be carried out. In this paper, we present some aspects of this mission and details of the payload that will be used and the scientific expectations. Copyright ©2010 by the International Astronautical Federation. All rights reserved.

The aster mission: Exploring for the first time a triple system asteroid

2001 SN263 is a triple system asteroid. Although it was discovery in 2001, in 2008 astronomical observation carried out by Arecibo observatory revealed that it is actually a system with three bodies orbiting each other. The main central body is an irregular object with a diameter about 2.8 km, while the other two are small objects with less than 1 km across. This system presents an orbital eccentricity of 0.47, with perihelion of 1.04 and aphelion of 1.99, which means that it can be considered as a Near Earth Object. This interesting system was chosen as the target for the Aster mission - first Brazilian space exploration undertaking. A small spacecraft with 150 kg of total mass, 30 kg of payload with 110 W available for the instruments, is scheduled to be launched in 2015, and in 2018 it will approach and will be put in orbit of the triple system. This spacecraft will use electric propulsion and in its payload it will carry image camera, laser rangefinder, infrared spectrometer, mass spectrometer, and experiments to be performed in its way to the asteroid. This mission represents a great challenge for the Brazilian space program. It is being structured to allow the full engagement of the Brazilian universities and technological companies in all the necessary developments to be carried out. In this paper, we present some aspects of this mission, including the transfer trajectories to be used, and details of buss and payload subsystems that are being developed and will be used. Copyright ©2010 by the International Astronautical Federation. All rights reserved.

Characterization of flavonoids and naphthopyranones in methanol extracts of Paepalanthus chiquitensis herzog by HPLC-ESI-IT-MSn and their mutagenic activity

A HPLC-ESI-IT-MSn method, based on high-performance liquid chromatography coupled to electrospray negative ionization multistage ion trap mass spectrometry, was developed for rapid identification of 24 flavonoid and naphthopyranone compounds. The methanol extracts of the capitulae and scapes of P. chiquitensis exhibited mutagenic activity in the Salmonella/microsome assay, against strain TA97a. © 2013 by the authors.

Produção de biomassa, rendimento e composição do óleo essencial de manjericão (Ocimum basilicum L.) em função de reguladores vegetais

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análise dos fotoiniciadores presentes em resinas compostas em função da cor

Pós-graduação em Ciências Odontológicas - FOAR

Avaliação do perfil farmacocinético do florfenicol em plasma bovino após aplicação intramuscular de duas doses e avaliação da sua eficácia a bactérias sensíveis

Pós-graduação em Ciência Animal - FMVA

Produção de fitomassa, teor e composição química do óleo essencial de Baccharis dracunculifolia DC. em função da adubação orgânica

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Caracterização da diversidade genética e da composição química dos óleos essenciais de populações de Ocimum selloi Benth

Pós-graduação em Agronomia (Horticultura) - FCA