843 resultados para n-3 LC-PUFA biosynthesis enzymes


Relevância:

30.00% 30.00%

Publicador:

Resumo:

Botryosphaeria rhodina MAMB-05 produced β-1,3-glucanases and botryosphaeran when grown on glucose, while Trichoderma harzianum Rifai only produced the enzyme. A comparison of long-term cultivation (300h) by B. rhodina demonstrated a correlation between the formation of botryosphaeran (48h) and its consumption (after 108h), and de-repression of β-1,3-glucanase synthesis when glucose was depleted from the nutrient medium, whereas for T. harzianum enzyme production commenced during exponential growth. Growth profiles and levels of β-1,3-glucanases produced by both fungi on botryosphaeran also differed, as well as the production of β-1,3-glucanases and β-1,6-glucanases on glucose, lactose, laminarin, botryosphaeran, lasiodiplodan, curdlan, Brewer's yeast powder and lyophilized fungal mycelium, which were dependent upon the carbon source used. A statistical mixture-design used to optimize β-1,3-glucanase production by both fungi evaluated botryosphaeran, glucose and lactose concentrations as variables. For B. rhodina, glucose and lactose promoted enzyme production at the same levels (2.30UmL -1), whereas botryosphaeran added to these substrates exerted a synergic effect favorable for β-glucanase production by T. harzianum (4.25UmL -1). © 2010 Elsevier B.V.

Relevância:

30.00% 30.00%

Publicador:

Resumo:

A fibrolytic enzyme complex was added to the pre-starter diet. Broiler chicks were randomly distributed into five treatments, consisting of a diet with no enzyme addition and four test diets supplemented with 100, 200, 300 and 400g/T of an enzyme complex. The dietary inclusion of the enzyme complex increased weight gain, and the dose of 300g/T improved weight gain and worsened feed conversion ratio.

Relevância:

30.00% 30.00%

Publicador:

Resumo:

Suplemento al Manual del Usuario y al Manual de Generación de Bases de Datos

Relevância:

30.00% 30.00%

Publicador:

Resumo:

Contiene los campos que se invierten para generar el archivo invertido sobre el cual se realizan búsquedas de información.

Relevância:

30.00% 30.00%

Publicador:

Resumo:

Explica los procedimientos a seguir para realizar las funciones de crear o poblar bases de datos, con el resultado de una búsqueda o con el total de una base de datos.

Relevância:

30.00% 30.00%

Publicador:

Resumo:

The physiological state of yeast cells changes during culture growth as a consequence of environmental changes (nutrient limitations, pH and metabolic products). Cultures that grow exponentially are heterogeneous cell populations made up of cells regulated by different metabolic and/or genetic control systems. The strain of baker's yeast selected by plating commercial compressed yeast was used for the production of glycerol-3- phosphate dehydrogenase. Glycerol-3-phosphate dehydrogenase (GPD) has been widely used in the enzyme assays with diverse compounds of industrial interest, such as glycerol or glycerol phosphate, as well as a number of important bioanalytical applications. Each cell state determines the level of key enzymes (genetic control), fluxes through metabolic pathways (metabolic control), cell morphology and size. The present study was carried out to determine the effects of environmental conditions and carbon source on GPD production from baker's yeast. Glucose, glycerol, galactose and ethanol were used as carbon sources. Glycerol and ethanol assimilations required agitation, which was dependent on the medium volume in the fermentation flask for the greatest accumulation of intracellular GPD. Enzyme synthesis was also affected by the initial pH of the medium and inoculum size. The fermentation time required for a high level of enzyme formation decreased with the inoculum size. The greatest amount of enzyme (0.45 U/ml) was obtained with an initial pH of 4.5 in the medium containing ethanol or glycerol. The final pH was maintained in YP-ethanol, but in the YP-glycerol the final pH increased to 6.9 during growth.

Relevância:

30.00% 30.00%

Publicador:

Resumo:

Error en símbolo en tapa y contraportada (S/2006/20)

Relevância:

30.00% 30.00%

Publicador:

Resumo:

Contiene la organizacion de los trabajos, resumen de los debates y conclusiones de la reunion que tuvo por objetivo analizar, por una parte, la instrumentacion de la Estrategia Internacional del Desarrollo (EID) y las perspectivas de America Latina y el Caribe para el fin del decenio de 1980, teniendo presente la evolucion de la crisis y, por la otra, la deuda externa de America Latina y el Caribe.