Morphology and morphometry of feline bone marrow-derived mesenchymal stem cells in culture

Mesenchymal stem cells (MSC) are increasingly being proposed as a therapeutic option for treatment of a variety of different diseases in human and veterinary medicine. Stem cells have been isolated from feline bone marrow, however, very few data exist about the morphology of these cells and no data were found about the morphometry of feline bone marrow-derived MSCs (BM-MSCs). The objectives of this study were the isolation, growth evaluation, differentiation potential and characterization of feline BM-MSCs by their morphological and morphometric characteristics. in vitro differentiation assays were conducted to confirm the multipotency of feline MSC, as assessed by their ability to differentiate into three cell lineages (osteoblasts, chondrocytes, and adipocytes). To evaluate morphological and morphometric characteristics the cells are maintained in culture. Cells were observed with light microscope, with association of dyes, and they were measured at 24, 48, 72 and 120h of culture (P1 and P3). The non-parametric ANOVA test for independent samples was performed and the means were compared by Tukey's test. On average, the number of mononuclear cells obtained was 12.29 (±6.05x10(6)) cells/mL of bone marrow. Morphologically, BM-MSCs were long and fusiforms, and squamous with abundant cytoplasm. In the morphometric study of the cells, it was observed a significant increase in average length of cells during the first passage. The cell lengths were 106.97±38.16µm and 177.91±71.61µm, respectively, at first and third passages (24 h). The cell widths were 30.79±16.75 µm and 40.18±20.46µm, respectively, at first and third passages (24 h).The nucleus length of the feline BM-MSCs at P1 increased from 16.28µm (24h) to 21.29µm (120h). However, at P3, the nucleus length was 26.35µm (24h) and 25.22µm (120h). This information could be important for future application and use of feline BM-MSCs.

Effect of particle morphology on the mechanical and thermo-mechanical behavior of polymer composites

Fiber reinforced polymer composites have been used in many applications, such as in automobile, aerospace and naval industries, due basically to their high strength-to-weight and modulus-to-weight, among other properties. Even though particles are usually not able to lead to the level of reinforcement of fibers, particle reinforced polymer composites have been proposed for many new applications due to their low cost, easy fabrication and isotropic properties. In this work, polymer composites were prepared by incorporating glass particles of different morphologies on poly(aryl sulfones) matrices. Particles with aspect ratios equal to 1, 2.5 and 10 were used. The prepared composites were characterized using electron microscopy and thermal analysis. Mechanical properties of the composites were evaluated using a four-point bending test. The thermo-mechanical behavior of the obtained composites was also investigated. The results showed that the morphology of the particles alter significantly the mechanical properties of composites. Particles with larger values of aspect ratio led to large elastic modulus but low levels of strain at failure. This result was explained by modeling the thermo-mechanical behavior of the composites using a viscoelastic model. Parameters of the model, obtained from a Cole-Cole type of plot, demonstrated that interactions at the polymer-reinforcing agent interface were higher for composites with large aspect ratio particles. Higher levels of interactions at interfaces can lead to higher degrees of stress transfer and, consequently, to composites with large elastic modulus, as experimentally observed.

Effect of additives, precursors and process conditions on particle morphology

The objective of the thesis was to develop methods to manufacture and control calcium carbonate crystal nucleation and growth in precipitation process. The work consists of experimental part and literature part that addresses theory of nucleation, crystallization and precipitation. In the experimental part calcium carbonate was precipitated using carbonization reaction. Precipitation was carried out in presence of known morphology controlling agents (anionic polymers and sodium silicate) and by using different operation conditions. Formed material was characterized using SEM images, and its thermal stability was assessed. This work demonstrates that carbon dioxide feeding rate and concentrations of calcium hydroxide and additives can be used to control size, shape and amount of precipitating calcium carbonate.

Effects of weed control through cowpea intercropping on mayze morphology and yield

Intercropping combined with competitive maize cultivars can reduce the use of herbicides to control weeds. The objective of this work was to evaluate the effects of intercropping cowpea and maize, as well as hand-weeding on maize morphology and yield. The experimental design was in randomized complete blocks, with treatments arranged in split-plots and five replications. The plots consisted of four maize cultivars (BA 8512, BA 9012, EX 4001, EX 6004) and the split-plots consisted of the following treatments: no-weeding; twice hand-weeding (20 and 40 days after sowing); and intercropping with cowpea ('Sempre Verde' cultivar), both maize and cowpea sown at the same time. The variables evaluated were: maize fresh green ears and grain yield; characteristics of internodes, leaves, tassels, ears, grains; plant height and ear insertion height; number of weed plants and species; fresh and dry biomass of weed species and cowpea. Ten weed species were outstanding during the experiment, many of them from the Poaceae family. No interactions were found between weed control method and maize cultivars for most variables evaluated; and plants from hand-weeded split-plots showed superior mean values compared to plants from non-weeded and intercropped split-plots, both not differing from each other. The cowpea was inefficient in controlling weed, reducing the maize yields and not producing any grain. The maize cultivars 'BA 8512' and 'BA 9012 showed the highest mean green ear yield, and the highest grain yield in hand-weeded, no-weeded and intercropped split-plots. On the other hand, the maize cultivar 'EX 6004' showed such high means only in no-weeded and intercropped split-plots. 'EX 4001 presented the worst means in these variables for hand-weeded, no-weeded ant intercropped split-plots.

SEED MORPHOLOGY IN OnobrychisMILLERSECTION HYMENOBRYCHIS DC. FROM TURKEY

ABSTRACT The seed (nutlet) morphology of four Onobrychis Miller (Fabaceae: subfamily Papilionoideae, Section Hymenobrychis DC.) taxa from Turkey, including three endemic taxa, was examined using scanning electron microscopy. Onobrychis tournefortii, O. galegifolia, O. cappadocica, O. albiflora. The seed examined exhibited variation in size, shape, colour, and surface sculpturing. Seed size ranged between 4.0-5.2 mm length and 2.0-3.6 mm width. Observed shapes included; ellipticus anguste asymmtricus reniformis, Ovatus anguste asymmetricus reniformis and ellipticus reniformis. Seed surface sculpturing revealed two distinct types: reticulate and rugulate. Species of Onobrychisare generally similar and confused with those of Hedysarumduring the identification process. Seed surface micromorphology can suggest taxonomical diagnostic characters for distinguishing species. Many of these characteristics are diagnostic at both the generic and specific levels.

Morphology and anatomy in Heliconia angusta Vell. and H. velloziana L. Emygd. (Zingiberales: Heliconiaceae) from the Atlantic forest of southeastern Brazil

Morphological and anatomical features of roots, stems, leaves, and scapes were studied in Heliconia angusta and H. velloziana from the Atlantic forest in the southeastern of Brazil. Morphologically H. angusta and H. velloziana show differences in their sizes, blade shapes, number and shape of inflorescence bracts. On the other hand, they have common anatomical characteristics such as: roots with air-canals in the cortex; rhizomes with isolated fiber bundles, collateral vascular bundles, and uniseriate endodermis and pericycle; leaves presenting air-canals and collateral vascular bundles forming arcs, and thin-walled epidermal cells; scapes with collateral vascular and fiber bundles in the cortex. The distribution of the fiber bundles in the leaves and in the scapes was different for each species, having a taxonomical value, H. velloziana presenting continuous fiber bundles. Air-canals in roots and leaves with narrow mesophyll might be related to the moist understorey of the Atlantic forest habitats.

Morphology and anatomy of the fruit and seed in development of Chorisia speciosa A. St.-Hil. - Bombacaceae

The structure of the fruit and seed in development of Chorisia speciosa are described with the main purpose of clarifying the origin and nature of the hairs that cover the seeds and aiding future taxonomical and ecological studies of the group. The fruit is an ellipsoid loculicide capsule and presents the exocarp formed by 7-10 cells layers, with very thick walls and evident simple pits. A great number of mucilage secretory cavities and ramified vascular bundles, accompanied by fibers, occur in the parenchymatic mesocarp. The endocarp derives from the ventral epidermis of the ovary wall, whose cells undergo a gradual elongation, become lignified, and constitute the trichomes which cover the mature seeds. The fruit aperture occurs by means of a suture evident in the ovarian wall in the middle region of the carpel leaf. Anatropous and bitegmic ovules, provided by a hypostase, give rise to campilotropous and bitegmic seeds. The testa is uniseriate, the exotegmen is completely formed by macrosclereids, and mucilage secretory cavities occur in the mesotegmen. The endotegmen, which is differentiated in the endothelium, is crushed in the mature seed. The plicate embryo, which occupies practically the entire seminal cavity, is found between endosperm layers, both being rich in lipids.

Morphology, reproduction and development of Hypoglossum hypoglossoides (Stackhouse) Collins & Hervey (Ceramiales, Rhodophyta) from the south and southeastern Brazilian coast

Hypoglossum hypoglossoides (Stackhouse) Collins & Hervey is reported for the first time from the infralittoral of São Paulo and Santa Catarina states. The species was already reported to the states of Rio de Janeiro, Espírito Santo and Bahia as Hypoglossum tenuifolium (Harvey) J. Agardh var. carolinianum Williams. A detailed description of the morphology and reproduction is given based on field-collected material. Unialgal cultures were initiated from tetraspore germination, and growth rates of gametophytes were determined under different conditions of temperature, photoperiod and irradiance. Gametophytes grew well between 15 to 30 ºC, 14L:10D and 10L:14D photoperiods and irradiance from 20 to 120 µmol photons.m-2.s-1, but presented low percentage of fertile plants in low temperature (15 ºC). Gametophytes cultured in laboratory developed only male reproductive structures. Physiological responses of H. hypoglossoides indicate that the species is well adapted to temperature and light variations which could explain its range of vertical (6-26 m depth) and latitudinal distribution (from Fernando de Noronha to Santa Catarina) as well as the absence of sexual reproduction in the southern limit of its distribution.

Morphology and reproduction of Predaea feldmannii Børgesen (Nemastomataceae, Rhodophyta), an uncommon species from Brazil

The coast of Espirito Santo State is located in a biogeographic transition zone presenting high diversity. A renewed interest in this region occurred in the 1990s, resulting in various new records and new species descriptions added to the Brazilian marine flora. The study of the infralitoral of this region is just beginning, and a detailed exploration with use of Scuba diving is revealing a flora containing many little-known taxa, particularly of red algae. As a first result, Predaea feldmannii Børgesen is being described for the Brazilian coast. The occurrence of the genus Predaea in Brazil has been considered with restriction since representatives of this genus had been referred only once by Howe & Taylor in 1931, being first considered as Platoma. The occurrence of Predaea in Brazil is now confirmed, and its vegetative and reproductive structures are described in detail. Predaea feldmannii can be recognized by having gonimoblast initial formed on a bulge of the connecting filament, cortical filaments of nearly equal lengths, lack of gland cells, dense clusters of nutritive cells borne only on cells immediatelly contiguous to the auxiliary cell, and a three-celled carpogonial branch.

Morphology and histochemistry of glandular trichomes of Cordia verbenacea DC. (Boraginaceae) leaves

The objective of this work was to study, using light and fluorescence microscopy and scanning electron microscopy, the morphology and secretory products of glandular trichomes of Cordia verbenacea DC. (Boraginaceae), known as 'baleeira', a species used in folk medicine as anti-inflammatory, analgesic, anti-ulcerogenic and healing agent. Two classes of glandular trichomes were recognized, globular and reniform. A morphological study of the secretory head and the characterization of the secretory product are also presented. Secretory products of globular trichomes consisted of essential oils, whereas reniform trichomes consisted basically of phenolic compounds such as flavonoids. No pre-established regions for releasing secretory products were found.

Pollen morphology of species of Hortia (Rutaceae)

A palinological study covering all 10 species of the Neotropical genus Hortia Vand. (Rutaceae) was conducted. Pollen grains were collected from herbarium exsiccates, acetolysed and mounted in glycerine jelly on glass slides. The grains were studied under light and scanning electron microscopy, including measurements of polar and equatorial diameters, shape of the grains, number and shape of apertures, and thickness of the exine. The results demonstrated that the genus is stenopalynous, with pollen grains in monads, subprolate to prolate, 3-colporate, with very thick exine and a psilate-perforate pattern of ornamentation. Pollen grains of all species revealed a great similarity, with few variations in the pattern of ornamentation of exine, number and form of apertures and measurements. Although well-characterized palinologycally, pollen features did not furnish relevant information on the position of Hortia into an intrafamilial phylogeny.

Morphology and reproduction of Mesophyllum erubescens (Foslie) Me. Lemoine (Corallinales, Rhodophyta) from Southern Brazil

The genus Mesophyllum Me. Lemoine includes around 147 species, of which only three have been referred to the Brazilian coast. Mesophyllum erubescens was originally described from Fernando de Noronha Archipelago, Brazil (type locality). Here we present the first detailed description of M. erubescens based on Brazilian material. Samplings were made through scuba diving at the Biological Marine Reserve of Arvoredo Island, Santa Catarina. The relations of M. erubescens with other similar species, especially from the American Atlantic studied by W.R. Taylor are discussed.

Morphology and anatomy of the developing fruit of Maclura tinctoria, Moraceae

(Morphology and anatomy of the developing fruit of Maclura tinctoria, Moraceae). Maclura tinctoria (L.) D. Don ex Steudel was selected for the present study due of its economic and medicinal importance. The purpose of this investigation is to present a detailed description of the fruit development, specially by: (a) defining the fruit type presented by the species, and (b) characterizing the seed type of the species based upon the presence or not of mechanical tissue on the seed-coat. The fruit originates from the subglobose female inflorescence which consists of small unipistillate flowers with superior ovary, unilocular and uniovular apical placentation. The mature fruit is multiple, constituted of small drupes. The ovule is ana-campylotropous, suspended, bitegmic and crassinucellate. The mature seed is flattened, slightly ovated, cream colored, with unspecialized membrane coat with thin-walled cells more or less crushed. The seed has parenchymatic endosperm with lipophilic content. The embryo is straight, with two cotyledons of the same size. Ontogenetic studies reveal that the fruits are infrutescences. The fleshy edible part is derived from the perigone and inflorescence axis. The drupes consist of a single pyrene of macrosclereids.

Genetic interactions involving major genes at the dwarf locus in egg-type chickens

Three egg-type stocks segregating dwarf (dw) and bantam (dwB) genes in female progeny were produced from the same 18 heterozygous (dwB/dw) sires used to inseminate dams of three different genotypes: normal (dw+), dwarf (dw) and bantam (dwB) dams. The heritability of 8-week body weight estimated from full-sibs of the same phenotype of progeny was 0.40, and that estimated from paternal half-sibs of the same phenotype (dwarf or bantam), and from the same genotype of dam was 0.38. Therefore, maternal and non-additive effects within genotypic classes of dam made little contribution to the genetic variance for 8-week body weight among their progeny. The interaction of sires (S) with genotypes (dw+, dw and dwB) of dam (G) was significant at the 5% level. This indicates that the rankings of the sires within each one of the three genotypes of dam were not the same, probably due to non-additive genetic variation among genotypes of dams. The evidence indicated that in general the genes from individual sires combined differently with each type of dam (G). Those genes which combined well with the genes from normal (dw+) dams combined poorly with both the genes from the dwarf (dw) and the genes from the bantam (dwB) dams. The interaction of sires (S) with phenotypes (dwarf and bantam) of progeny (P) was significant at the 10% level. The results indicated a probable gene x genotype interaction for 8-week weight between genes at the dwarf locus (dw and dwB) and the background genotype (single and/or polygenes). The correlation among paternal half-sibs was influenced more by the S x G than by the S x P interaction, but the effects tended to be cumulative

Synthesis of stocks and phenotypic effects of dwarf and bantam sex-linked major genes in egg-type chickens

The present study describes the production of stocks segregating dwarf (dw), bantam (dwB) and normal (dw+) alleles, as well as the characters, shank length, adult body weight, age at sexual maturity and egg production. Heterozygous K dw+/k dwB sires were mated to normal (dw+) dams to produce stock D6.a, and mated to dwB females to produce stock D6.b. Stock D4.a came from mating F1 heterozygous dwB dw sires to dwarf Leghorns. In a third series of matings, 7/8 Sebright and 1/8 dw-Leghorn dwB dw sires were crossed to three groups of dams of different genotypes. The progeny of the normal (dw+), dwarf (dw), and bantam (dwB) dams were designated as stocks D4.b, D4.c and D4.d, respectively. The dw+ dams were White Leghorn strain cross females. The difference between the rate of laying of normal (69.7%) and their bantam sisters (68.6%) was not statistically significant when the average 32-week body weight of the dw+ sisters was 1,897 g. However, when the 32-week body weight of the normal daughters from the same sires and smaller dams was around 1,646 g, the difference between the rate of laying of the normal (78.1%) and their bantam sisters (75.9%) was significant (P < 0.05). The dwB gene may have a similar but smaller effect on the rate of egg laying than its dwarf allele. The difference between sexual maturity of normal and bantam daughters of either the largest or the smallest dams was not statistically significant, even though the smallest dwB pullets were in average 2.9 days older at first egg. The use of shank length combined with adult body weight allowed a precise discrimination between bantams and dwarfs