A Proteomic Study on Cell Cycle Progression of Endothelium Exposed to Tumor Conditioned Medium and the Possible Role of Cyclin D-1/E

This study was designed to comprehensively analyze the differential expression of proteins from human umbilical vein endothelial cells (HUVECs) exposed to tumor conditioned medium (TCM) and to identify the key regulator in the cell cycle progression. The HUVECs were exposed to TCM from breast carcinoma cell line MDA-MB-231, then their cell cycle distribution was measured by flow cytometer (FCM). The role of protein in cell cycle progression was detected via two-dimensional polyacrylamide gel electrophoresis (2-DE) and western blotting. Following the stimulation of TCM, HUVECs showed a more cells in the S phase than did the negative control group (ECGF-free medium with 20% FBS), but the HUVECs' level was similar to the positive control group (medium with 25 mug/ml ECGF and 20% FBS). Increased expression of cyclin D-1/E and some changes in other related proteins occurred after incubation with TCM. From our results, we can conclude that breast carcinoma cell line MDA-MB-231 may secrete soluble pro-angiogenic factors that induce the HUVEC angiogenic switch, during which the expression of cell cycle regulator cyclin D-1/E increases and related proteins play an important role in this process.

P.v.b. virgillii maronis : bucolicorum, eclogaex., georgicorum, libri iii., aeneidos, libri xii. et in ea, mauri servii honorati : grammatici comentarii, ex antiquiss : exemplaribus longe meliores et auctiores : ex bibliotheca petri danielis i.c. : accessit fabij planciadis fulgentij liber de continentia virgiliana, auctior e mss. codd. item iunij philargyrij commentariolus in bucolica & georgica virgilij. : cum certissimo et copiosissimo indice

As Bucólicas são constituídas de dez éclogas, consideradas como traduzidas ou imitadas de Teócrito. As Geórgias são um poema didático no qual se acentua a predileção de Virgílio pela vida campestre. A Eneida é uma utopia política que o poeta não conseguiu acabar e foi publicada contra a sua vontade expressa.

Io. gottl. heineccii, iureconsulti quondam celeberrimi : recitationes in elementa iuris civilis secundum ordinem institutionum, accedunt io. christ. gottl. heineccii ... commentarius de vita, fatis ac scriptis b. parentis, auctior emendatior christiani thomasii, delineatio historiae juris nec non burc. gottelf. struvii, bibliotheca juris selectissima cum notis hen. contelmanni, ubi praestantiora iurisconsultorum opera ad nostra usque tempora referuntur

Título Uniforme : Corpus Juris Civilis. Institutas.

Motor vessel "N. B. Scofield" cruise one of 1953. Cruise Report 53-S-1

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Cruise no. 2 of the M/V N.B. Scofield for 1957, 57S2-Albacore. Cruise report 57-S-2

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El vídeo-tutorial: una valiosa herramienta para el b-learning

Ponencia leída en el Foro de Comunicaciones IkasArt III (BEC Barakaldo, 2011.11.11)

Hugonis grotii de jure belli ac pacis :libri tres, cum annotatis auctoris, nec non j.f. gronovii notis, & j. barbeyracii animadversionibus, commentariis insuper locupletissimis henr. l.b. de cocceii, sacrae regiae majestati borussicae, quondam a consiliis secretioribus, sub titulo grotii illustrati antea editis, nunc ad calcem cujusque capitis adjectis : insertis quoque observationibus samuelis l.b. de coceii, henrici fillii, regni borussici cancellarii, ... adduntur tandem ipsius grotti dissertatio de mari libero, ac libellus singularis de aequitate, indulgentia et facilitate

Titulo do volume 5: Samuelis l.b. de cocceii, summi regni borussici canncellari... introductio ad Henrici l.b. de cocceii grotium illustratum.

Cruise 2 of the N. B. Scofield for 1951. Cruise Report 51-S-2

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Cruise 3 of the N. B. Scofield for 1951. Cruise Report 51-S-3

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Cruise 4 of the N. B. Scofield for 1951. Cruise Report 51-S-4

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Cruise 5 of the N. B. Scofield for 1951. Cruise Report 51-S-5

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Cruise 6 of the N. B. Scofield for 1951. Cruise Report 51-S-6

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Cruise 7 of the N. B. Scofield for 1951. Cruise Report 51-S-7

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Cruise 8 of the N. B. Scofield for 1951. Cruise Report 51-S-8

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Cruise 1 of the N. B. Scofield for 1952. Cruise Report 52-S-1

(PDF contains 3 pages.)