Funding was obtained from a Capes Fellowship to NM, grants CNPq Universal 481351/ 2011-6, CNPq PQ 306604/2012-4, FAPERN/CNPq Pronem 003/2011, Capes SticAmSud, and FAPESP/CEPID/Neuromat to S.R. CNPq Universal 473554/2011-9 and 480053/ 2013-8, CNPq PQ 308558/2011-1, FACEPE/CNPq-PRONEX APQ- 0203-1.05/08, FACEPE/CNPq-PRONEM APQ-1415-1.05/10, and CNAIPS to M.C

Funding was obtained from a Capes Fellowship to NM, grants CNPq Universal 481351/ 2011-6, CNPq PQ 306604/2012-4, FAPERN/CNPq Pronem 003/2011, Capes SticAmSud, and FAPESP/CEPID/Neuromat to S.R. CNPq Universal 473554/2011-9 and 480053/ 2013-8, CNPq PQ 308558/2011-1, FACEPE/CNPq-PRONEX APQ- 0203-1.05/08, FACEPE/CNPq-PRONEM APQ-1415-1.05/10, and CNAIPS to M.C

Molecular analysis of the bacterial diversity in a specialized consortium for diesel oil degradation

Diesel oil is a compound derived from petroleum, consisting primarily of hydrocarbons. Poor conditions in transportation and storage of this product can contribute significantly to accidental spills causing serious ecological problems in soil and water and affecting the diversity of the microbial environment. The cloning and sequencing of the 16S rRNA gene is one of the molecular techniques that allows estimation and comparison of the microbial diversity in different environmental samples. The aim of this work was to estimate the diversity of microorganisms from the Bacteria domain in a consortium specialized in diesel oil degradation through partial sequencing of the 16S rRNA gene. After the extraction of DNA metagenomics, the material was amplified by PCR reaction using specific oligonucleotide primers for the 16S rRNA gene. The PCR products were cloned into a pGEM-T-Easy vector (Promega), and Escherichia coli was used as the host cell for recombinant DNAs. The partial clone sequencing was obtained using universal oligonucleotide primers from the vector. The genetic library obtained generated 431 clones. All the sequenced clones presented similarity to phylum Proteobacteria, with Gammaproteobacteria the most present group (49.8 % of the clones), followed by Alphaproteobacteira (44.8 %) and Betaproteobacteria (5.4 %). The Pseudomonas genus was the most abundant in the metagenomic library, followed by the Parvibaculum and the Sphingobium genus, respectively. After partial sequencing of the 16S rRNA, the diversity of the bacterial consortium was estimated using DOTUR software. When comparing these sequences to the database from the National Center for Biotechnology Information (NCBI), a strong correlation was found between the data generated by the software used and the data deposited in NCBI.

Análise espacial dos fatores da equação universal de perda de solo em área de nascentes

O objetivo deste trabalho foi avaliar a perda de solo de área de nascentes da Microbacia do Córrego do Tijuco, SP. Foi utilizada a análise espacial dos fatores da equação universal da perda de solo (EUPS), em integração com análise de componentes principais e geoestatística. A perda de solo média, estimada para a área, foi de 118,5 Mg ha‑1 por ano, considerada alta. Próximo à zona urbana, houve alta interação dos fatores erosividade da chuva e práticas conservacionistas, o que evidencia grande perda de solo, em razão da concentração da água proveniente da camada impermeabilizada urbana, com alta velocidade de escoamento. Nos divisores de águas, a atuação da erodibilidade foi proeminente, em contraste com o fator topográfico. Foram observadas áreas com atuação conjunta destes fatores, inclusive em locais de inclinação suave, porém com alto potencial natural de erosão. A interação das análises multivariadas e geoestatística permite a estratificação da área, identifica locais com propriedades específicas quanto à perda de solo, e espacializa os fatores do processo erosivo e suas interações ao longo do relevo.

Os jogos com regras na perspectiva do desenho universal: contribuições à educação matemática inclusiva

To think about a school that is for everyone has been a challenge for many people connected to education worldwide demanding from researchers of each level of knowledge an association to such effort. The study presented on this paper unites itself to the voices, movements and researches of these scholars, seeking to contribute on building possibilities on which mathematics can be thought and worked on schools in order for every student to learn, whether they have some sort of deficiency, disorders, syndromes or not. This essay has the goal to investigate the possibilities of inclusive pedagogical practices mediated by math games with rules, developed and used throughout the Universal Design perspective; a qualitative research took place with a collaborative methodology that involved managers, teachers and students from a public school situated on the city of Natal/Brazil. On the investigation math games with rules were developed and made according to the Universal Design concept, starting from initial studies which articulated theoretical groundings to the reality of school and the teacher s conceptions. After that, classes using these tools were planned collectively which oriented inclusive pedagogical practices of classes from the 1st to the 4th year of elementary school. Throughout the process many instruments such as: tape recording, video footages, notes from the researcher; the teachers and the students were used for constant work evaluation and also to record the research data. In the end, the data indicated effective contributions of the mediated pedagogical practices by games with rules under the perspective of Universal Design for Inclusive Mathematics Education

Um sistema computacional para diagnosticar viroses de plantas usando a técnica de PCR com construção de primers espécie-específicos

It proposes a established computational solution in the development of a software to construct species-specific primers, used to improve the diagnosis of virus of plant for PCR. Primers are indispensable to PCR reaction, besides providing the specificity of the diagnosis. Primer is a synthetic, short, single stranded piece of DNA, used as a starter in PCR technique. It flanks the sequence desired to amplify. Species-specific primers indicate the well known region of beginning and ending where the polymerase enzyme is going to amplify on a certain species, i.e. it is specific for only a species. Thus, the main objective of this work is to automatize the process of choice of primers, optimizing the specificity of chosen primers by the traditional method

Begomovírus infectando a cultura de pimentão no estado de São Paulo

Vírus do gênero Begomovirus são transmitidos por mosca-branca Bemisia tabaci G., e constituem um dos problemas fitossanitários sérios em diversas culturas. Plantas de pimentão coletadas em oito regiões do Estado de São Paulo, foram submetidas a extração de DNA total e PCR com primers universais e degenerados para begomovírus, que amplificam parte da região codificadora para a proteína capsicial. Os dados indicam a presença de begomovírus em pimentão nas cinco regiões coletadas. Análise das seqüências do DNA viral e análise filogenética revelaram identidade com dois begomovírus nativo da América. Tomato severe rugose virus - ToSRV (AY029750) e com Tomato yellow vein streak virus (ToYVSV, AY829113), espécies descritas infectando tomateiro no Brasil. A presença de begomovírus em pimentão foi verificada nas regiões de Alvinlândia, Ubirajara, Botucatu, Elias-Fausto, Paulínia, Mogi Guaçu, Paranapanema e Pirajú.

Entre a fé, a obra social e a publicidade: uma análise crítica do discurso da responsabilidade social da Igreja Universal do Reino de Deus

Situated on Applied Linguistics (PENNYCOOK, 1998; MOITA LOPES, 2003, 2006, 2008, 2009), this thesis, which is inscribed in a qualitative-interpretative approach of a critical analysis perspective , lies on the speech of social responsibility and the way like that is employed in seeking for legitimacy and prestige within the neopentecostal brazilian religious field, more specifically of the Universal Church of the Kingdom of God. The general goal of this research is t reflect upon the speech on social responsibility and the rethoric of selfpromotion of the Universal Church through the role of social actors in the making of his her identities, materialized in the newspaper Folha Universal. In order to achieve that, we have conjugated, in this research, social and discursive analysis. On the linguistic-discursive approach, the research is based on the Critical Analysis of Discourse (ACD), specially in Fairclough (2001, 2003, 2006), a proposal that provides theoretical-methodological tools to investigate the language beyond the linguistic structures , that is, the discourse, social practices in which it occurs and more ample structures. Theoretical assumptions were also used of Sistemico-Functional Linguistics(LSF), matching with categories of the Transitivity System of Halliday (1994, 2004), of the forms of representation of social actors in the socioeconomic perspective by Van Leeuwen (1997, 2008) and of the Appraisal system by Martin and White (2005). As we develop the argumentation on thesobre social role of religion in this thesis, we make use of the authors such as Freston (1994), Oro (1997, 2003), Campos (1996, 1999), Mariano (1999), Meneses (2008), among several ones. We have also used a series of concepts and categories coming from the field of communication and marketing on the business social responsability and social marketing . In this area, we take as references the contributions of Bueno (2003), Fossá and Sartoretto (2003) and Zenone (2006). The corpus of the work is framed by news taken at the newspaper Folha Universal, in which are given the social responsibility actions of the church . The timeframe used was on the editions of 2010 thru 2012. Results found at he analysis of the News lead to semantic features of Assessment of Affection, Judgment and Appreciation, many times followed by Gradation, and the Attribution, one of the subsystems of the Attachment, are evidence of positive assessments for the Universal Church and its agents and make up rethorical elements which provide structure for the discourse of the Universal Church at the newspaper Folha Universal consisted of its image (style) of social responsibility . Results show that the most frequente social actors of the discourse are, on one hand, the Universal Church itself and its volunteers, famous (actors, actresses, presenters), politicians and authorities, on the other hand, the population which was helped by the Church social projects . The first group seems to be Always activated, however the second one, most of the time rather passive. These are also represented by assimilation in most of the occurrences, however the other ones by individualization and nomination entitled by honorification, except for the volunteers that are represented either as an individual, or as a group

Bond strength between acrylic resin and maxillofacial silicone

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

EFFECT of METAL PRIMERS on BOND STRENGTH of RESIN CEMENTS TO BASE METALS

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Flexibility and Torsional Strength of ProTaper and ProTaper Universal Rotary Instruments Assessed by Mechanical Tests

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

New primers for the amplification and sequencing of nuclear loci in a taxonomically wide set of reptiles and amphibians

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Universal scaling in Bardeen-Cooper-Schrieffer superconductivity in two dimensions in non-s waves

The solutions of a renormalized BCS model are studied in two space dimensions for s, p and d waves for finite-range separable potentials. The gap parameter, the critical temperature T-c, the coherence length xi and the jump in specific heat at T-c as a function of the zero-temperature condensation energy exhibit universal scalings. In the weak-coupling limit, the present model yields a small xi and large T-c, appropriate for high-T-c cuprates. The specific heat, penetration depth and thermal conductivity as functions of temperature show universal scaling for p and d waves.