Epitopes close to the apolipoprotein B low density lipoprotein receptor-binding site are modified by advanced glycation end products

Advanced glycation end products (AGEs) are thought to contribute to the abnormal lipoprotein profiles and increased risk of cardiovascular disease of patients with diabetes and renal failure, in part by preventing apolipoprotein B (apoB)-mediated cellular uptake of low density lipoproteins (LDL) by LDL receptors (LDLr). It has been proposed that AGE modification at one site in apoB, almost 1,800 residues from the putative apoB LDLr-binding domain, may be sufficient to induce an apoB conformational change that prevents binding to the LDLr. To further explore this hypothesis, we used 29 anti-human apoB mAbs to identify other potential sites on apoB that may be modified by in vitro advanced glycation of LDL. Glycation of LDL caused a time-dependent decrease in its ability to bind to the LDLr and in the immunoreactivity of six distinct apoB epitopes, including two that flank the apoB LDLr-binding domain. ApoB appears to be modified at multiple sites by these criteria, as the loss of glycation-sensitive epitopes was detected on both native glycated LDL and denatured, delipidated glycated apoB. Moreover, residues directly within the putative apoB LDLr-binding site are not apparently modified in glycated LDL. We propose that the inability of LDL modified by AGEs to bind to the LDLr is caused by modification of residues adjacent to the putative LDLr-binding site that were undetected by previous immunochemical studies. AGE modification either eliminates the direct participation of the residues in LDLr binding or indirectly alters the conformation of the apoB LDLr-binding site.

Identification of genes required for Drosophila eye development using a phenotypic enhancer-trap

A novel method of P-element mutagenesis is described for the isolation of mutants affecting the development of the Drosophila compound eye. It exploits the interaction between the Bride of Sevenless (Boss) ligand and the Sevenless (Sev) receptor tyrosine kinase that triggers the formation of the UV-sensitive photoreceptor neuron, R7. Transposition of a boss cDNA transgene, in an otherwise boss mutant background, was used as a “phenotypic trap” in live flies to identify enhancers expressed during a narrow time window in eye development. Using a rapid behavioral screen, more than 400,000 flies were tested for restoration of R7. Some 1,800 R7-containing flies were identified. Among these, 21 independent insertions with expression of the boss reporter gene in the R8 cell were identified by a external eye morphology and staining with an antibody against Boss. Among 900 lines with expression of the boss reporter gene in multiple cells assessed for homozygous mutant phenotypes, insertions in the marbles, glass, gap1, and fasciclin II genes were isolated. This phenotypic enhancer-trap facilitates (i) the isolation of enhancer-traps with a specific expression pattern, and (ii) the recovery of mutants disrupting development of specific tissues. Because the temporal and tissue specificity of the phenotypic trap is dependent on the choice of the marker used, this approach can be extended to other tissues and developmental stages.

Trabajos de seguimiento de la lapa ferruginosa Patella ferruginea Gmelin, 1791 en Andalucía en el marco de la Estrategia Nacional de Conservación de la especie

El gasterópodo marino Patella ferruginea se encuentra incluido en los Catálogos Español y Andaluz de Especies Amenazadas en la categoría “En peligro de extinción”. En 2008 fue aprobada la Estrategia de Conservación Nacional de la especie que establece la realización de un seguimiento de la población cada cuatro años. En Andalucía se ha realizado en 2010 el seguimiento de la especie empleando dos tipos de metodología: los “Controles de crecimiento”, mediante marcaje de ejemplares, y los “Censos exhaustivos” en “Tramos” de costa, para intentar detectar todos los individuos presentes. En el censo de 2010 se han muestreado unos 21 km de costa en 34 localidades, un 5% del litoral andaluz con presencia de la especie, lo que constituye un esfuerzo considerable, pero asumible para el control periódico de la misma. La densidad media detectada es muy baja, de 0,048 ind./m. El mayor número de individuos se encuentra en Cádiz y la población mejor estructurada en la isla de Alborán. Se estima que el tamaño actual de la población en Andalucía ronda los 1.800 ejemplares, lo que constituye un aumento con respecto a inventarios anteriores. Sin embargo, el contingente es muy reducido para garantizar la supervivencia de la especie. La categoría de protección propuesta por el Libro Rojo de los Invertebrados de Andalucía, “En peligro crítico” (MORENO y ARROYO, 2008), debe considerarse, por lo tanto, la más adecuada para la lapa ferruginosa siguiendo los criterios de valoración de la UICN (2001).

La economía callejera en las ciudades contemporáneas. La redes efímeras de venta ambulante como modelo para la reconstrucción de lo urbano

Según Robert Neuwirth alrededor de 1.800 millones de personas trabajan en la economía informal, generando unos 10 billones de dólares anuales. Este autor afirma que si todas las personas vinculadas a dicho modelo económico se reunieran en un único país, éste sería la segunda potencia económica mundial. Junto a la importancia económica del modelo, su flexibilidad al contexto urbano es también destacable. La economía callejera, en particular, está vinculada a la construcción de redes urbanas complejas donde el individuo puede decidir y negociar. Este aspecto es lo que hace de este tipo de apropiación urbana una referencia fundamental para la innovación en la construcción de ciudad y, en especial, para el Urbanismo Táctico. En este artículo se analiza cómo las construcciones urbanas vinculadas a la economía callejera y a la venta ambulante se relacionan con los conceptos que diversos autores han empleado para definir las dinámicas contemporáneas. Primero se aborda la manera en que la venta ambulante teje, relaciona y transforma las redes urbanas existentes (sociales, productivas, económicas y de identidad) y busca su vínculo con los siguientes conceptos: ‘red’, ‘rizoma’, ‘radicante’, ‘punto de paso obligado’ y ‘hacker’. Seguidamente se reflexiona sobre la materialización de la economía informal en las ciudades actuales, relacionando este fenómeno con los siguientes conceptos: ‘el derecho a la ciudad’, ‘lo informal’, ‘TAZ’ y el urbanismo ‘emergente’, entre otros.

Ecuador, 1892 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Carta geográfica del Ecuador, por Teodoro Wolf ; publicada por órden del supremo gobierno de la república y trabajada bajo las presidencias de los EE. señores D.J.M. Plácido Caamaño y D. Antonio Flores. It was published by Instituto geográfica de H. Wagner & E. Debes in 1892. Scale 1:445,000. Covers coastal Ecuador and portions of Colombia and Peru. Map in Spanish. The image inside the map neatline is georeferenced to the surface of the earth and fit to the South America Lambert Conformal Conic projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, railroads, roads, territorial boundaries, shoreline features, and more. Relief shown by shading and spot heights. Includes also insets: La region oriental del Ecuador -- Archipiélago de Galápagos, and table of altitudes of cities, villages and other inhabited spots, and, a table administrative divisions and subdivisions of Ecuador. This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Central & Eastern Africa, 1887 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Central Africa : on a scale of 1:10,000,000, By Dr. F. Boas. It was published by Hart & Von Arx in 1887. Scale 1:10,000,000 The image inside the map neatline is georeferenced to the surface of the earth and fit to the Africa Sinusoidal projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, shoreline features, and more. Relief shown by shading. This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Rome, Italy, 1891 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Pianta di Roma, col patrocinio del comune di Roma. It was published by editore Loreto Pasqualucci [per] dall' Istituto cartografico italiano in 1891. Scale 1:6,000. Map in Italian.The image inside the map neatline is georeferenced to the surface of the earth and fit to the European Datum 1950, Universal Transverse Mercator (UTM) Zone 33N projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as roads, railroads and stations, drainage, built-up areas and selected buildings, walls, gates, fortification, ground cover, and more. Relief shown by shading. Includes also index and inset: Receniti di Roma nei successivi periodi della sua storia.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Brussels, Belgium, 1907 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Plan général de Bruxelles et des communes suburbaines, dressé par M. Frosty ; gravé par A. Verwest. It was published by Librairie Falk Fils in 1907. Scale 1:10,000. Covers a portion of Brussels, Belgium. Map in French.The image inside the map neatline is georeferenced to the surface of the earth and fit to the Belgian Lambert 1972 coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as roads, railroads and stations, street-railroads, drainage, built-up areas and selected buildings, parks, ground cover, city districts, and more.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Berlin, Germany, 1920 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Kiessling's grosser plan von Berlin : mit Voroten und Völlständiger Stadt - u. Ringbahn. It was published by Alexius Kiessling in 1920. Scale 1:20,000. Covers a portion of Berlin, Germany. Map in German. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Deutsches Hauptdreiecksnetz (DHDN) 3-degree Gauss-Kruger Zone 4 coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as roads, railroads and stations, drainage, selected buildings, parks, ground cover, city districts, and more. Relief shown by spot heights.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Palermo, Italy, 1891 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Nuova pianta della città di Palermo. It was published by Carlo Clausen, editore in 1891. Scale 1:10,000. Covers a portion of Palermo, Italy. Map in Italian.The image inside the map neatline is georeferenced to the surface of the earth and fit to the European Datum 1950, Universal Transverse Mercator (UTM) Zone 33N projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as roads, railroads and stations, drainage, built-up areas, selected buildings, parks, ground cover, shoreline features, and more. Includes index. Relief shown by shading.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Berlin, Germany, 1895 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Plan von Berlin : mit sämtlichen Vororten und vollständiger Stadt- und Ringbahn, bearbeitet und herausgegeben von Julius Straube ; Geographisches Institut und Landkarten. It was published by Geographisches Institut und Landkarten-Verlag Jul. Straube in 1895. Scale 1:17,777. Covers Berlin, Germany. Map in German.The image inside the map neatline is georeferenced to the surface of the earth and fit to the Deutsches Hauptdreiecksnetz (DHDN) 3-degree Gauss-Kruger Zone 4 coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as roads, railroads and stations, street railways, drainage, built-up areas and selected buildings, city districts, parks, and more.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Bristol, England, 1890 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Plan of Bristol, drawn, engraved by G.W. Bacon & Co. New and rev. ed. It was published by James Fawn & Son, ca. 1890. Scale 1:10,560. The image inside the map neatline is georeferenced to the surface of the earth and fit to the 'British National Grid' coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as roads, railroads and stations, street railway lines, drainage, built-up areas and selected buildings, cemeteries, parks, and more. This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Coasts, West Scotland, United Kingdom, 1800 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: The Lewis and west coast of Scotland : from the best authorities, by Stuart Amos Arnold, and other experienced navigators. It was published by D. & E. Steele, at the Navigation Warehouse, Little Tower Hill in Jany. 1st, 1800. Scale [1:275,000]. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Europe Lambert Conformal Conic coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, shoreline features (rocks, shoals, anchorage points, ports, inlets, etc.), and more. Relief shown by hachures and pictorially; depths shown by soundings. Includes index.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Cork, Ireland, 1771 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: A survey of the city and suburbs of Cork, By J. Rocque, 1759. It was printed for Robert, Sayer, No. 53 in Fleet Street in 1771. Scale 1:2,400.The image inside the map neatline is georeferenced to the surface of the earth and fit to the Irish Grid (Transverse Mercator 1965 (TM-65)) coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as roads, drainage, built-up areas and selected buildings, ground cover, parks, gardens, docks, and more. Relief shown by shading.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Central Europe Region, 1859 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Neueste Eisenbahn-Karte von Central-Europa : mit genauer Angabe aller Stationen und den influirenden Post-Verbindungen, nach dem neuesten und zuverlässigsten Quellen bearbeitet von U. Hendschel. It was published by Carl Jügel's Verlag in 1859. Scale 1:1,900,000. Covers the Central Europe region. Map in German.The image inside the map neatline is georeferenced to the surface of the earth and fit to the Europe Lambert Conformal Conic coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, roads, railroads, territorial and administrative boundaries, shoreline features, and more. Relief shown by hachures. Includes also scale comparison chart and index of political units.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.