Fator de condição e biologia alimentar de Pimelodus maculatus em três pontos do Rio Piracicaba (SP)

A number of 170 individuals of the species Pimelodus maculatus, a Neotropical fish known as mandi was collected in three distinct locations of the Piracicaba river (Piracicaba, Santa Maria da Serra and Americana – SP0 from April 2009 to February 2010. A common species of catfish whose individuals exist in many neotropical basins was observed to get more information about a possible influence of the water conditions in distant parts of a same river to its condition and also to verify whether the stomach contents of fish collected in these distinct places may distinguish as a possible consequence of food availability. The species were studied by collecting biological data such as standard length (SL) in cm, their total weights (TW ) in grams and sexes to see the composition of fish present in the samples. Length-weight relationships are important means to investigate facts related to the condition of the species in these systems. The analysis was made by using covariance to observe if individuals of similar lengths collected in distinct places may be compared to this purpose. Usual methods to investigate relationships of stomach food contents were applied. By analyzing the condition factor, one may conclude that the mandis collected in the region of Santa Maria da Serra presented similar characteristics to those obtained in Piracicaba, but they differ in this aspect for individuals collected in Americana. This fact is not conclusive about the reasons of this situation, but it may be a first step to other studies in order to get more information about. The degree of stomach repletion may be a first approach to this possible explanation, as individuals of Piracicaba presented usually full stomachs and differed in this way to individuals collected in the two other places

Aplicação da acupuntura em animais silvestres

This study will focus on one part of Traditional Chinese Medicine (TCM), acupuncture - utilized as therapy method for pathologies treatment in humans and animals - , as well as its main techniques. Acupuncture is one branch of TCM, originated from theoretical and empirical knowledge accumulated by popular medicine. Currently, researches performed in humans and animals have shown the effectiveness of this method as an assistance or alternative in illnesses treatment. Besides that, professionals are not merely looking for orthodox medicine knowledge, but also specializations and improvement over the complementary therapies area. There are diverse studies and researches which were and are being done in veterinary, relating the effectiveness of acupuncture in small and large animals. In comparison, in the wild animals’ area the numbers of studies is smaller. However, it is expected that this number increases over the fact that some of these animals’ species are entering the pet market and also because of the possibility of using these methods for treatment and prevention of diseases on zoo animals or other animals kept in captivity

Perfil sanitário e pesquisa de Salmonella em frangos e lingüiças pela metodologia tradicional e pela PCR

Novos hábitos alimentares da população e as mudanças nos sistemas de produção e distribuição dos alimentos podem estar fortemente relacionados aos casos de doenças transmitidas pelo consumo de alimentos contaminados por microorganismos. Isso faz com que a preocupação com a qualidade microbiológica dos alimentos aumente mundialmente. Dentre os principais patógenos, destaca-se a Salmonella sp, que pertence à microbiota gatrentestinal de animais silvestres e de animais domésticos criados para o consumo humano (aves, bovinos e suínos). Este fato torna o problema de grande relevância para as autoridades de saúde pública, pois esses microrganismos podem estar associados às infecções ou surtos provocados pela ingestão de alimentos contaminados. Foram coletadas 35 amostras de lingüiças e 25 cortes de frango, nos principais supermercados do município de Botucatu-SP e avaliados quanto à qualidade higiênico-sanitária através da determinação do número mais provável de coliformes termotolerantes (CT), além da presença de Salmonella sp. Os resultados obtidos mostram que 31% das lingüiças e 84% das amostras de frango estavam fora dos padrões higiênico-sanitários estabelecido pela ANVISA (RDC n° 12), que determina uma quantidade máxima de 5x103 CT/g em lingüiça e 104 /g em carne de aves. Em relação à Salmonella sp, estabeleceu-se a ausência dessa bactéria em 25g de lingüiça e não determina nenhum parâmetro para o frango. Entre as 25 amostras de frango analisadas, duas (8%) foram positivas pela metodologia tradicional. Esse... (Resumo completo, clicar acesso eletrônico abaixo)

Métodos analíticos para a identificação de espécies animais

The food industry has been rapidly modernized, and with this the disposal and the consumption of industrialized food has been increasing continually. These products lose their original morphological characteristics, requiring fast and reliable tests that could help to identify the species in question, as most fraudulent behavior in the milk and dairy industry (meat and fish) is carried out where there is partial or total exchange of the original material for other with less value at market. Nowadays there is a lot of techniques that can be used for the identification of animal species, based on muscle protein, or DNA analysis. In the case of protein based analysis, we can mention several types of electrophoresis and immunologic methods, as ELISA. In the case of DNA based methods, we have several assays that use the amplification of DNA fragments, known as PCR, as proof. All these techniques have advantages and disadvantages that can be affected by factors- the sample condition, or the degree of relation between the species in question. Because of this, it’s necessary that a continuous study looking for the improvement of the available techniques, making sure that the confirmation of food authenticity is in place. This is to ensure the true product value, to comply with labeling regulationand and protect the consumer of frauds

Cicatrização cutânea por segunda intenção em pequenos animais

This study has the objective to show the physiologic process involved in the small animals wound healing, including his phases, types of wounds, the topic products, like anti-septic and ointments, and his mains indications for wound by second intention. In the actual market exists the increasing emergence of topic using products, vegetable healing ointments , being plants extracts or oils despite of a searching for more efficient products, fasters and cheaper, although don´t know much about the effects of these products What are the new types of treatment for wounds expected for the future

Endocardite infecciosa em pequenos animais

Infective endocarditis is a process in which an infection attacks the heart endothelial surface, and is commonly caused by bacterial colonization, which is called bacterial endocarditis. It is a condition rarely found in dogs and cats, and is more prevalent in male dogs of large size. It mainly affects the left side of the heart, affecting the mitral and aortic valves with greater frequency. The circulation of the bacterium in the bloodstream is what gives rise endocarditis, and is caused by any non-aseptic process that serves as a gateway for bacterium in the body, as from a skin lesion, even as an invasive procedure, such as, catheterization and surgery. The ante-mortem diagnosis is difficult because the clinical signs of endocarditis are varied and common to other diseases, summing up the signs of infection (fever, lethargy, weight loss), and presence of heart murmur and may show signs of congestive heart failure. Thus, the diagnosis is most often through autopsy. To arrive at a diagnosis should be used, besides the history and physical examination, some laboratory tests, especially blood cultures and echocardiography. Treatment is accomplished through the use of antibiotics for long period of time, it is very important to follow the results of susceptibility after its outcome is revealed. The prognosis for bacterial endocarditis ranges from guarded to poor, and can be assessed mainly by the echocardiography. There are few studies in veterinary about the bacterial endocarditis, and the majority is case reports

Desenvolvimento da técnica de MALDI Imaging utilizando tecidos animais

Currently the study of important molecular compounds present in low abundance in some tissues has been a challenge for proteomic analysis classic. An analysis requires more exploratory investigation of small regions of a tissue or a group of cells. MALDI Imaging Technology (MSI) is an application of mass spectrometry facing the chemical analysis of intact tissues. Thus, advances in mass spectrometry MALDI being obtained by the integration of histology, the best methods and automation are the main tools of data analysis. This tool has become essential to analyze the spatial distribution of peptides and proteins throughout the tissue sections, providing an enormous amount of data with minimum sample preparation. Thus, the aim of this study was to develop the technique of MALDI Imaging using tissue from glioblastoma multiforme (GBM), a form of most common malignant tumor in the brain. For this we used the printer chemical ChIP-1000 (Chemical Inkjet Printer, Shimadzu) and mass spectrometer type Maldi-ToF-ToF (Axima Performance, Shimadzu), a search of the identifications were performed in databases such as SwissProt. We identified more than forty proteins with diverse functions such as proteins F-actin-capping and Thymosin to the structure and organization cellular and proteins such several Tumor necrosis factor receptor development-related pathology. The development of this technique will permit to carry-out proteomic analysis directly into the tissue, enabling earlier diagnosis of diseases, as well as the identification and characterization of potential biomarkers of disease.

Importância de aves na dispersão de semenetes em áreas restauradas do interior de São Paulo

A prática da Restauração Ecológica teve um aumento considerável nas ultimas décadas no Brasil, a fim de remanejar a integridade ecológica dos ecossistemas degradados. No entanto, em virtude da prática ser ainda recente faz-se necessário maiores estudos na área para compreender a relação e a importância dos frugívoros nas redes de interações, bem como as mudanças no desenvolvimento da biodiversidade durante o processo de restauração, visando otimizar os resultados de práticas futuras. Desse modo, o presente trabalho teve como finalidade analisar a estrutura da comunidade de aves do sub-bosque, bem como determinar as principais espécies envolvidas no processo de dispersão de sementes realizado por elas em três áreas restauradas do interior de São Paulo (Iracemápolis, Santa Bárbara d’Oeste e Cosmópolis), com diferentes idades de plantio (14, 25, 57 anos respectivamente), tendo uma área nativa como referência. Para isso foram realizadas redes de neblina para capturar as aves do sub-bosque, e coletar amostras de fezes, afim de se analisar possíveis sementes no conteúdo estomacal/intestinal. Constatou-se que a espécie de Turdus leucomelas foi a mais abundante em todas áreas de estudo, e a mais expressiva para a dispersão de sementes. Iracemápolis e Santa Bárbada d’Oeste apresentaram a maior taxa de captura de indivíduos, enquanto que Cosmópolis apresentou a maior similaridade da avifauna com a mata nativa. Em relação à distribuição das guildas alimentares da avifauna nas áreas, predominaram-se espécies insetívoras em detrimento da baixa presença de frugívoros. Em conclusão, há uma importância significativa da restauração em áreas degradadas para recrutar a avifauna, mesmo que a maioria dos indivíduos sejam de espécies menos sensíveis à fragmentação

Falência renal aguda em pequenos animais

The pets, mostly dogs and cats are susceptible to a variety of situations that can lead to acute kidney failure, such as infection and intoxication, besides other common causes such as dehydration or other pre-existing diseases. The abrupt decrease in renal function in these animals characterizes a severe clinical condition and requires specialized veterinary medical care since it increases the death. Early diagnosis is an important factor for the survival of these animals and so is the appropriate treatment. New technologies on diagnosis and treatment of serious diseases in veterinary medicine have made acute renal failure successfully reversed by hemodialysis in cases where conventional therapy may not be effective. In cases like that, hemodialysis treatment may be the only chance of survival for the animals, which makes this therapy more and more important and necessary, especially in referral centers of veterinary assistance

Caracterização da fauna recebida e avaliação dos procedimentos em Centros de Triagem de Animais Silvestres (CETAS)

Estima-se que anualmente, em função do tráfico de animais silvestres, cerca de 12 milhões de espécimes sejam retirados de nossas florestas, sendo que apenas uma ínfima porcentagem de indivíduos é recuperada e destinada à soltura com o devido rigor técnico. O tráfico de animais silvestres constitui um dos fatores mais relevantes da destruição da fauna, acarretando em um desequilíbrio na estruturação e manutenção dos ecossistemas. Este comércio ilegal constituise o terceiro maior do mundo, perdendo apenas para o tráfico de narcóticos e armas. Assim, para otimizar o processo de soltura da fauna como estratégia para conservação de espécies torna-se necessário uma análise quantitativa e qualitativa da fauna atendida e dos procedimentos realizados nos Centros de Triagem de Animais Silvestres (CETAS). Nesse sentido, o presente trabalho teve como objetivo fazer uma caracterização da fauna recebida e uma avaliação dos procedimentos realizados em dois CETAS, além de discutir sobre a importância desse órgão na triagem, reabilitação e conservação de animais silvestres. O presente estudo é justificado pela escassez de informações sobre a fauna retirada da natureza. Estas são necessárias para servir de base científica para a adoção de políticas públicas para o combate ao tráfico de animais silvestres; elaborar um programa de fauna que contemple a triagem e destinação adequada e contribuir para o desenvolvimento de trabalhos educativos com o fim de esclarecimento da sociedade. Foram analisados o “Livro de Registro” do CETAS da Fundação Animália e o “Livro de Plantão” do CETAS DEPAVE – 3, bem como os “Relatórios CETAS” que esses dois Centros enviaram ao IBAMA. O período escolhido foi do ano de 2003 a 2008. Os dados foram compilados e organizados em planilhas do Microsoft Office Excel e foram elaborados tabelas e gráficos para análise dos resultados.

Síndromes Paraneoplásicas em Pequenos Animais

Paraneoplastic syndromes are being increasingly recognized in dogs and cats in veterinary medicine as they often are oncological emergencies and may help in early diagnosis of primary neoplasm, performing a more effective treatment, which usually is the removal of the tumor, and thereby improving the quality of life of the animal and perhaps prognosis. The syndrome is caused by the tumor through the production of substances such as hormones and cytokines that are released into circulation and are responsible for the appearance of distant signals from the primary neoplasm. These syndromes can involve multiple systems of the body, among them we mention: the hematology, the dermatologist, the neurologist, endocrine and musculoskeletal

Aspectos gerais da síndrome anorexia caquexia do câncer em pequenos animais

The cancer anorexia-cachexia syndrome is the most common paraneoplastic syndrome in Veterinary Medicine. It is characterized by severe loss of muscle mass and adipose tissue resulting in severe unintentional weight loss, anemia, fatigue, negative nitrogen balance, immune dysfuntion and other metabolic disturbances. The SAC is not only a result of inadequate intake of nutrients. The tumor requires large amounts of nutrients to allow growth and causes changes in pacient metabolism to get this energy. Recent studies suggest that the metabolic changes by cancer can be measured by hormones and cytokines produced or by the patient or the tumor, but this not completely understood. Animals with SAC have lower survival time, the greater chance of complications during treatment and lower quality of life. With the increase in the number of cancer cases in domestic animals and longer lifespan after diagnosis of malignant disease through the use of antineoplastics drugs, diagnosis and treatment of cancer anorexia-cachexia syndrome has shown great importance in that patients may have higher survival then better quality of life. This paper aims to provide information about this complex and multifunctional syndrome and its possible treatments

Plantas alimentares de raízes e tubérculos na agricultura familiar: um estudo de caso como enfoque etnobotânico com agricultores do município de Rio Claro

A erosão genética de espécies domesticadas, provocada pelos processos associados a Revolução Verde despertou o interesse mundial pela agrobiodiversidade manejada em sistemas agrícolas de pequena escala onde situam-se agricultores familiares. Propostas de conservação in situ e/ou on farm têm sido elaboradas. No Brasil, a agricultura familiar tem grande importância na produção de alimentos e a mandioca (Manihot esculenta Crantz) é a espécie que se sobressai, sendo a agricultura familiar responsável por 87% da sua produção. O presente estudo teve como objetivos: um levantamento etnobotânico dos cultivos alimentícios de raízes e tubérculos de agricultores familiares do município de Rio Claro, São Paulo; e analisar aspectos sócio-econômicos visando estratégias para a conservação in situ de variedades por eles cultivadas e potencial continuidade das atividades pelas gerações futuras. Foram entrevistados 31 agricultores, em sua maioria homens, usando o método “Bola de neve”, distribuídos entre nove bairros ou distritos rurais da cidade de Rio Claro. As entrevistas realizadas para coleta dos dados foram semi-estruturadas, tanto para dados sócioeconômicos como para os dados sobre as variedades reconhecidas pelos agricultores. As entrevistas foram realizadas nos sítios dos agricultores e suas áreas de cultivo foram visitadas, onde foram feitas coletas botânicas. Os dados foram analisados de forma qualitativa utilizando-se gráficos e tabelas e descrições do estudo de caso. A espécie predominante da amostra foi a mandioca. Os agricultores mostraram possuir fatores positivos que os caracterizam como mantenedores da diversidade agrícola, como o fato de cultivarem etnovariedades de mandioca diversas e terem um sistema de caracterização e reconhecimento delas de base empírica, contudo, estes agricultores perderam, em sua maioria... (Resumo completo, clicar acesso eletrônico abaixo)

O discurso do uso de animais nas pesquisas de Educação Física

It is possible in many countries, perhaps all, there is business groups of pharmaceuticals industry, cosmetics together with the universities that use animals in scientific research and testing for the creation of new products and medicines. Currently is growing discussion about standards and criteria for the use of these animals (guinea pigs) and also increases the number of debates on ethical issues in research with animal models. This research seeks to portray the reality of the use of animals in scientific research academic at the University, specifically to research in physical education, as it has used animals in the areas of Physiology of Sport and Physical Activity, Nutrition for Athletes, among others. Initially is shown the pre-reflection, exposing the laws governing the subject, treating the animal experimentation with regard to its history and the issues of speciesism and the methodological mistake. It reveals what it is and as is the use of animals in education and the inability of the student to change that reality, to refuse to participate in the cruel activity, and even they are aware of their rights as in the case of the withdrawal of consciousness. Presents researchers and universities not use animals and ends the pre-debate showing Who is really the most animal research at the university by providing alternatives for them. We conducted a literature survey on animal experimentation, research universities, and alternative methods. The following is the phenomenon in understanding and awareness of the researchers who use animals in their research, conducting a phenomenological analysis of his speeches, revealing the meanings of their animal research. The focus of this study is, therefore, the analysis of discourses of researchers who carried out research using animal experiments (guinea pigs). The phenomenon of understanding of the researchers about their research is made clear... (Complete abstract click ectronic access below)

Avaliação da influência do enriquecimento ambiental nas variáveis comportamentais de saguis-de-cara-branca (Callithrix geoffroyi) em cativeiro

Six species of the genus Callithrix can be found across the Atlantic Forest extension, being the Callithrix geoffroyi, Geoffroy`s marmoset, the least studied species. The objective of this study is to investigate the behavioral aspects of the species in captivity, and how the environmental enrichment techniques influence those behaviors. To this end, we have studied three specimens of C. geoffroyi, in captivity at Municipal Zoo of Piracicaba, São Paulo. The enclosure includes a 37m2 with under masonry, concrete platforms with external deep, trunks, a floor of grass and soil, e grid in their other three sides. The sampling method was focal per time interval, with records every 30 seconds in sessions of 30 minutes, totaling 30 hours for each animal, which were distributed in a baseline phase, an environmental enrichment phase and post-enrichment phase (n = 3660 records in each phase). The twelve enrichment techniques were applied: the physical environment (branches for perches and hoses fabrics for bedding and vanishing points), cognitive (radio, mirror, puzzle food) and food (insects, flowers and eggs hidden, wrapped). Initially, to determine the period of greatest activity of marmosets, we investigated the pattern of activity for three days (9:00 am to 17:00 pm) consecutively at intervals of 15 minutes, recording the number of active and inactive individuals. The 495 records signaled greater activity in the morning, then selected period for the completion of this work. The ethogram comprised 16 behaviors. The inactivity was the predominant phase behavior baseline and post-enrichment with a significant decrease (h=8,62, p <0.01) in the enrichment phase, and a significant increase in the post enrichment (h=18,15, p <0.001). The same was seen for the use of substrate (grid and ground vs. Trunk and concrete, h=5,09, p <0.001 and h=3,98, p <0.05)... (Complete abstract click electronic access below)