Synthesis of the stationary phase IS-Anionic (internal surface-anionic) for extraction of ochratoxin A and B from samples of beers

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Non-stationary frequency analysis of extreme daily rainfall in São Paulo, Brazil

This work is an assessment of frequency of extreme values (EVs) of daily rainfall in the city of São Paulo. Brazil, over the period 1933-2005, based on the peaks-over-threshold (POT) and Generalized Pareto Distribution (GPD) approach. Usually. a GPD model is fitted to a sample of POT Values Selected With a constant threshold. However. in this work we use time-dependent thresholds, composed of relatively large p quantities (for example p of 0.97) of daily rainfall amounts computed from all available data. Samples of POT values were extracted with several Values of p. Four different GPD models (GPD-1, GPD-2, GPD-3. and GDP-4) were fitted to each one of these samples by the maximum likelihood (ML) method. The shape parameter was assumed constant for the four models, but time-varying covariates were incorporated into scale parameter of GPD-2. GPD-3, and GPD-4, describing annual cycle in GPD-2. linear trend in GPD-3, and both annual cycle and linear trend in GPD-4. The GPD-1 with constant scale and shape parameters is the simplest model. For identification of the best model among the four models WC used rescaled Akaike Information Criterion (AIC) with second-order bias correction. This criterion isolates GPD-3 as the best model, i.e. the one with positive linear trend in the scale parameter. The slope of this trend is significant compared to the null hypothesis of no trend, for about 98% confidence level. The non-parametric Mann-Kendall test also showed presence of positive trend in the annual frequency of excess over high thresholds. with p-value being virtually zero. Therefore. there is strong evidence that high quantiles of daily rainfall in the city of São Paulo have been increasing in magnitude and frequency over time. For example. 0.99 quantiles of daily rainfall amount have increased by about 40 mm between 1933 and 2005. Copyright (C) 2008 Royal Meteorological Society

Theoretical study on the reaction mechanism of VO2+ with propyne in gas phase

Possible molecular mechanisms of the gas-phase ion/molecule reaction of VO2+ in its lowest singlet and triplet states ((1)A(1)/(3)A '') with propyne have been investigated theoretically by density functional theory (DFT) methods. The geometries, energetic values, and bonding features of all stationary and intersystem crossing points involved in the five different reaction pathways (paths 1-5), in both high-spin (triplet) and low-spin (singlet) surfaces, are reported and analyzed. The oxidation reaction starts by a hydrogen transfer from propyne molecule to the vanadyl complex, followed by oxygen migration to the hydrocarbon moiety. A hydride transfer process to the vanadium atom opens four different reaction courses, paths 1-4, while path 5 arises from a hydrogen transfer process to the hydroxyl group. Five crossing points between high- and low-spin states are found: one of them takes place before the first branching point, while the others occur along path 1. Four different exit channels are found: elimination of hydrogen molecule to yield propynaldehyde and VO+ ((1)Sigma/(3)Sigma); formation of propynaldehyde and the moiety V-(OH2)(+); and two elimination processes of water molecule to yield cationic products, Prod-fc(+) and Prod-dc(+) where the vanadium atom adopts a four- and di-coordinate structure, respectively.

Fixed points and vacuum energy of dynamically broken gauge theories

We show that if a gauge theory with dynamical symmetry breaking has nontrivial fixed points, they will correspond to extrema of the vacuum energy. This relationship provides a different method to determine fixed points.

Uses of QCD sumrules (QCDSR) from different points of view

We support the idea that the baryon, B with mass MB, couples to its current with a coupling λ2 B ∼ 0.71 M6 B from an analysis of magnetic moment sum rules. And we find a sum rule among the experimental magnetic moments which is independent of the parameters of QCDSR. © 1998 Elsevier Science B.V. All rights reserved.

In vitro antimicrobial activity of different gutta-percha points and calcium hydroxide pastes

The aim of this study was to evaluate the antimicrobial activity of different trademarks and compositions of gutta-percha points and calcium hydroxide pastes used in endodontic therapy. The evaluated material consisted of gutta-percha points containing calcium hydroxide (Roeko™), gutta-percha points containing chlorhexidine (Roeko™), two convencional gutta-percha points (Endo Points™ and Roeko™) and two calcium hydroxide pastes (Calen™ and Calen/PMCC™). Antimicrobial tests included five species of microorganisms: Escherichia coli (ATCC10538), Staphylococcus epidermidis (ATCC12228), Staphylococcus aureus (ATCC6538), Pseudomonas aeruginosa (ATCC27853), and Micrococcus luteus (ATCC9341). The Agar difusion method was employed. The plates were kept at room temperature for 2 h for prediffusion and then incubated at 37°C for 24 h. The triphenyltetrazolium chloride gel was added for optimization and the zones of inhibition were measured. Statistical evaluation was carried out using analysis of variance and Tukey Test. The obtained results showed that all microbial species used in the study were inhibited by the gutta-percha points containing chlorhexidine and by the calcium hydroxide pastes (Calen™ and Calen/ PMCC™), with similar results (p > 0.05). No antimicrobial activity was observed for the other groups. It was concluded that the gutta-percha points containing chlorhexidine presented antimicrobial activity, whereas the gutta-percha points containing calcium hydroxide did not.

Influence of brush type as a carrier of adhesive solutions and paper points as an adhesive-excess remover on the resin bond to root dentin

Purpose: To evaluate the influence of the brush type as a earner of priming adhesive solutions and the use of paper points as a remover of the excess of these solutions on the push-out bond strength of resin cement to bovine root dentin. The null hypotheses were that brush type and the use of paper points do not affect the bond strength. Materials and Methods: The canals of 80 single-root bovine roots (16 mm in length) were prepared at 12 mm using the preparation drill (FRC Postec Plus, Ivoclar). Half of each root was embedded in acrylic resin and the specimens were divided into 8 groups, considering the factors brush type (4 levels) and paper point (2 levels) (n = 10): Gr 1: small microbrush (Cavi-Tip, SDI); Gr 2: Microbrush (Dentsply); Gr 3: Endobrush (Bisco); Gr 4: conventional brush (Bisco); Gr 5: Cavi-Tip (SDI) + paper points; Gr 6: Microbrush (Dentsply) + paper points; Gr 7: Endobrush (Bisco) + paper points; Gr 8: conventional brush (Bisco) + paper points. The root dentin was treated with a multistep total-etch adhesive system (All Bond 2). The adhesive system was applied using each microbrush, with and without using paper points. One fiber post was molded with addition silicon and 80 posts were made of resin cement (Duolink), The resin posts were luted (Duolink resin cement), and the specimens were stored for 24 h in water at 37°C. Each specimen was cut into 4 disk-shaped samples (1.8 mm in thickness), which were submitted to the push-out test. Results: The brush type (p < 0.0001) (small microbrush > microbrush = endobrush = conventional brush) and the use of paper points (p = 0.0001) (with > without) influenced the bond strength significantly (two-way ANOVA). The null hypotheses were rejected. Conclusion: The smallest brush (Cavi-Tip) and the use of paper points significantly improved the resin bond to bovine root dentin.

Interplanetary natural routes via Moon and Lagrangiam points L1 and L2

Swing-by techniques are extensively used in interplanetary missions to minimize fuel consumption and to raise payloads of spaceships. The effectiveness of this type of maneuver has been proven since the beginning of space exploration. According to this premise, we have explored the existence of a natural and direct links between low Earth orbits and the lunar sphere of influence, to obtain low-energy interplanetary trajectories through swing-bys with the Moon and the Earth. The existence of these links are related to a family of retrograde periodic orbits around the Lagrangian equilibrium point L1 predicted for the circular, planar, restricted three-body Earth-Moon-particle problem. The trajectories in these links are sensitive to small disturbances. This enables them to be conveniently diverted reducing so the cost of the swing-by maneuver. These maneuvers allow us a gain in energy sufficient for the trajectories to escape from the Earth-Moon system and to stabilize in heliocentric orbits between the Earth and Venus or Earth and Mars. On the other hand, still within the Earth sphere of influence, and taking advantage of the sensitivity of the trajectories, is possible to design other swing-bys with the Earth or Moon. This allows the trajectories to have larger reach, until they can reach the orbit of other planets as Venus and Mars.(3σ)Broucke, R.A., Periodic Orbits in the Restricted Three-Body Problem with Earth-Moon Masses, JPL Technical Report 32-1168, 1968.

Optimal low cost transfer to L4 and L5 lagrangian points

Lagrangian points L4 and L5 lie at 60 degrees ahead of and behind Moon in its orbit with respect to the Earth. Each one of them is a third point of an equilateral triangle with the base of the line defined by those two bodies. These Lagrangian points are stable for the Earth-Moon mass ratio. Because of their distance electromagnetic radiations from the Earth arrive on them substantially attenuated. As so, these Lagrangian points represent remarkable positions to host astronomical observatories. However, this same distance characteristic may be a challenge for periodic servicing mission. In this work, we introduce a new low-cost orbital transfer strategy that opportunistically combine chaotic and swing-by transfers to get a very efficient strategy that can be used for servicing mission on astronomical mission placed on Lagrangian points L4 or L5. This strategy is not only efficient with respect to thrust requirement, but also its time transfer is comparable to others known transfer techniques based on time optimization. Copyright ©2010 by the International Astronautical Federation. All rights reserved.

Optimal low-cost transfer to L4 and L5 lagrangian points based on G-family of periodic orbits

An alternative transfer strategy to send spacecrafts to stable orbits around the Lagrangian equilibrium points L4 and L5 based in trajectories derived from the periodic orbits around LI is presented in this work. The trajectories derived, called Trajectories G, are described and studied in terms of the initial generation requirements and their energy variations relative to the Earth through the passage by the lunar sphere of influence. Missions for insertion of spacecrafts in elliptic orbits around L4 and L5 are analysed considering the Restricted Three-Body Problem Earth- Moon-particle and the results are discussed starting from the thrust, time of flight and energy variation relative to the Earth. Copyright© (2012) by the International Astronautical Federation.

Saddle points and rare collisions under scaling approach in a Fermi accelerator with two nonlinear terms

Rare collisions of a classical particle bouncing between two walls are studied. The dynamics is described by a two-dimensional, nonlinear and area-preserving mapping in the variables velocity and time at the instant that the particle collides with the moving wall. The phase space is of mixed type preventing diffusion of the particle to high energy. Successive and therefore rare collisions are shown to have a histogram of frequency which is scaling invariant with respect to the control parameters. The saddle fixed points are studied and shown to be scaling invariant with respect to the control parameters too. © 2012 Elsevier B.V. All rights reserved.

Overall and differentiated session ratings of perceived exertion at different time points following a circuit weight training workout

Session ratings of perceived exertion (SRPE) have been considered to provide a quantitative evaluation of the entire exercise session in different types of resistance training. In this study we investigated the ability of SRPE to assess exercise strain in a circuit weight training (CWT) workout and the influence of time lag to report SRPE. Ten healthy male volunteers (22.3±2.8 years, 72.5±6.5kg, and 175±5cm) completed a CWT session involving three circuits of five multiple joint exercises with single sets of 20 repetitions at 30% one repetition maximum (1-RM). Heart rate [63.7-75.0% maximum heart rate (%HRmax)], blood lactate (5.6-7.6mM) as well as overall, chest, and active muscle RPE increased significantly (p<0.05) throughout the CWT, but no significant differences were found between ratings of perceived exertion (RPE) types. Overall, chest and active muscle SRPE were accessed 10 minutes, 20 minutes, and 30 minutes after the workout, with no significant main effects or SRPE type×time interaction being found (p>0.05). Finally, no significant differences (p>0.05) were observed between averaged SRPE and RPE responses (overall: 3.7±0.6 vs. 3.5±0.9; chest: 3.8±0.7 vs. 3.6±0.8; active muscle; 3.7±0.7 vs. 3.5±0.7). These results suggest SRPE, irrespective of the moment at which it is taken, to be a useful tool for assessing global exercise strain in a CWT workout, providing coaches, physicians, and exercisers a practical way for monitoring this type of resistance training. © 2013.

Genome-wide association study for birth weight in Nellore cattle points to previously described orthologous genes affecting human and bovine height

Background: Birth weight (BW) is an economically important trait in beef cattle, and is associated with growth- and stature-related traits and calving difficulty. One region of the cattle genome, located on Bos primigenius taurus chromosome 14 (BTA14), has been previously shown to be associated with stature by multiple independent studies, and contains orthologous genes affecting human height. A genome-wide association study (GWAS) for BW in Brazilian Nellore cattle (Bos primigenius indicus) was performed using estimated breeding values (EBVs) of 654 progeny-tested bulls genotyped for over 777,000 single nucleotide polymorphisms (SNPs).Results: The most significant SNP (rs133012258, PGC = 1.34 × 10-9), located at BTA14:25376827, explained 4.62% of the variance in BW EBVs. The surrounding 1 Mb region presented high identity with human, pig and mouse autosomes 8, 4 and 4, respectively, and contains the orthologous height genes PLAG1, CHCHD7, MOS, RPS20, LYN, RDHE2 (SDR16C5) and PENK. The region also overlapped 28 quantitative trait loci (QTLs) previously reported in literature by linkage mapping studies in cattle, including QTLs for birth weight, mature height, carcass weight, stature, pre-weaning average daily gain, calving ease, and gestation length.Conclusions: This study presents the first GWAS applying a high-density SNP panel to identify putative chromosome regions affecting birth weight in Nellore cattle. These results suggest that the QTLs on BTA14 associated with body size in taurine cattle (Bos primigenius taurus) also affect birth weight and size in zebu cattle (Bos primigenius indicus). © 2013 Utsunomiya et al.; licensee BioMed Central Ltd.