Digital storytelling: un’applicazione didattica per ripensare ai media attraverso i media

El presente trabajo de investigación propone un proyecto de investigación centrado en los pre-adolescentes para estudiar, a través deautobiografías digitales (digital storytelling, DST) producidas por ellos, laspracticas cultural-medial y las representaciones que ellos han construido sobre los medios, en relación con los contextos de uso significativos, como el hogar, la escuela o su grupo de par. Los medios forman parte de las actividades cotidianas de los niños quien los emplean en prácticas de socialización, aprendizaje, juego y de construcción de la identidad y del conocimiento. Los digital storytelling,insertados en el contexto escolar, serán empleados como metodología didácticapara la adquisición de competencias tecnológicas y como instrumento de lainvestigación. Los DST, como practica cultural de los chicos, constituirán nuestrocampo para la observación de las modalidades de uso de los medios por parte delos jóvenes y sus capacidad de recombinar lenguajes y construir contenidos. LosDST proporcionaran, además, la posibilidad de acceder al universo simbólicoinfantil para comprender el significado que las tecnologías tienen en sus cotidianidad

Ocular and systemic bio-distribution of rhodamine-conjugated liposomes loaded with VIP injected into the vitreous of Lewis rats.

PURPOSE: Local delivery of therapeutic molecules encapsulated within liposomes is a promising method to treat ocular inflammation. The purpose of the present study was to define the biodistribution of rhodamine-conjugated liposomes loaded with vasoactive intestinal peptide (VIP), an immunosuppressive neuropeptide, following their intravitreal (IVT) injection in normal rats. METHODS: Healthy seven- to eight-week-old Lewis male rats were injected into the vitreous with empty rhodamine-conjugated liposomes (Rh-Lip) or with VIP-loaded Rh-Lip (VIP-Rh-Lip; 50 mM of lipids with an encapsulation efficiency of 3.0+/-0.4 mmol VIP/mol lipids). Twenty-four h after IVT injection, the eyes, the cervical, mesenteric, and inguinal lymph nodes (LN), and spleen were collected. The phenotype and distribution of cells internalizing Rh-Lip and VIP-Rh-Lip were studied. Determination of VIP expression in ocular tissues and lymphoid organs and interactions with T cells in cervical LN was performed on whole mounted tissues and frozen tissue sections by immunofluorescence and confocal microscopy. RESULTS: In the eye, 24 h following IVT injection, fluorescent liposomes (Rh-Lip and VIP-Rh-Lip) were detected mainly in the posterior segment of the eye (vitreous, inner layer of the retina) and to a lesser extent at the level of the iris root and ciliary body. Liposomes were internalized by activated retinal Müller glial cells, ocular tissue resident macrophages, and rare infiltrating activated macrophages. In addition, fluorescent liposomes were found in the episclera and conjunctiva where free VIP expression was also detected. In lymphoid organs, Rh-Lip and VIP-Rh-Lip were distributed almost exclusively in the cervical lymph nodes (LN) with only a few Rh-Lip-positive cells detected in the spleen and mesenteric LN and none in the inguinal LN. In the cervical LN, Rh-Lip were internalized by resident ED3-positive macrophages adjacent to CD4 and CD8-positive T lymphocytes. Some of these T lymphocytes in close contact with macrophages containing VIP-Rh-Lip expressed VIP. CONCLUSIONS: Liposomes are specifically internalized by retinal Müller glial cells and resident macrophages in the eye. A limited passage of fluorescent liposomes from the vitreous to the spleen via the conventional outflow pathway and the venous circulation was detected. The majority of fluorescent liposomes deposited in the conjunctiva following IVT injection reached the subcapsular sinus of the cervical LN via conjuntival lymphatics. In the cervical LN, Rh-Lip were internalized by resident subcapsular sinus macrophages adjacent to T lymphocytes. Detection of VIP in both macrophages and T cells in cervical LN suggests that IVT injection of VIP-Rh-Lip may increase ocular immune privilege by modulating the loco-regional immune environment. In conclusion, our observations suggest that IVT injection of VIP-loaded liposomes is a promising therapeutic strategy to dampen ocular inflammation by modulating macrophage and T cell activation mainly in the loco-regional immune system.

Reference intervals for common carotid intima-media thickness measured with echotracking: relation with risk factors.

AIMS: Common carotid artery intima-media thickness (CCIMT) is widely used as a surrogate marker of atherosclerosis, given its predictive association with cardiovascular disease (CVD). The interpretation of CCIMT values has been hampered by the absence of reference values, however. We therefore aimed to establish reference intervals of CCIMT, obtained using the probably most accurate method at present (i.e. echotracking), to help interpretation of these measures. METHODS AND RESULTS: We combined CCIMT data obtained by echotracking on 24 871 individuals (53% men; age range 15-101 years) from 24 research centres worldwide. Individuals without CVD, cardiovascular risk factors (CV-RFs), and BP-, lipid-, and/or glucose-lowering medication constituted a healthy sub-population (n = 4234) used to establish sex-specific equations for percentiles of CCIMT across age. With these equations, we generated CCIMT Z-scores in different reference sub-populations, thereby allowing for a standardized comparison between observed and predicted ('normal') values from individuals of the same age and sex. In the sub-population without CVD and treatment (n = 14 609), and in men and women, respectively, CCIMT Z-scores were independently associated with systolic blood pressure [standardized βs 0.19 (95% CI: 0.16-0.22) and 0.18 (0.15-0.21)], smoking [0.25 (0.19-0.31) and 0.11 (0.04-0.18)], diabetes [0.19 (0.05-0.33) and 0.19 (0.02-0.36)], total-to-HDL cholesterol ratio [0.07 (0.04-0.10) and 0.05 (0.02-0.09)], and body mass index [0.14 (0.12-0.17) and 0.07 (0.04-0.10)]. CONCLUSION: We estimated age- and sex-specific percentiles of CCIMT in a healthy population and assessed the association of CV-RFs with CCIMT Z-scores, which enables comparison of IMT values for (patient) groups with different cardiovascular risk profiles, helping interpretation of such measures obtained both in research and clinical settings.

PlGF-1 and VEGFR-1 pathway regulation of the external epithelial hemato-ocular barrier. A model for retinal edema.

VEGF is considered as an important factor in the pathogenesis of macular edema. VEGF induces the rupture of the blood retinal barrier and may also influence the retinal pigment epithelial (RPE) outer retinal barrier. The aim of this work was to analyze the influence of the VEGF receptor pathways in the modulation of the RPE barrier breakdown in vitro and in vivo. The ARPE19 human junctions in culture are modulated by VEGF through VEGFR-1 but not through VEGFR-2. PlGF-1, that is a pure agonist of VEGFR-1, is produced in ARPE-19 cells under hypoxic conditions and mimics VEGF effects on the external retinal barrier as measured by TER and inulin flux. In vivo, the intravitreous injection of PlGF-1 induces a rupture of the external retinal barrier together with a retinal edema. This effect is reversible within 4 days. VEGF-E, that is a pure agonist of VEGFR-2, does not induce any acute effect on the RPE barrier. These results demonstrate that PlGF-1 can reproduce alterations of the RPE barrier occurring during diabetic retinopathy.

Premissas historicas do surgimento e desenvolvimento dos mass-media nos paises lusofonos

A nossa investigacao esta dedicada a paises que falam essencialmente a lingua portuguesa, nao sera de admirar que a maioria dos livros por nos usados sejam de autores e ediccao portuguesa. No decorrer deste trabalho recorremos a livros e trabalhos academicos de autores como: Carvalho, Arons de: A Censura À Imprensa Na Época Marcelista; Minerva,Coimbra, 1999; Evora, Silvino Lopes: As fontes jornalísticas na televisão caboverdiana, ediccoes FCC, Universidade do Minho,2004; Fontcuberta: Mar de, A Notícia – Pistas para compreender o mundo; Editorial Notícias “Tradução de Fernando Cascais”,Lisboa, 1999; Mata, Maria José: A Autocrítica no Jornalismo – O ombudsman na imprensa nacional e estrangeira; Minerva Editora,Coimbra, 2002; Pigeat, Henri: Médias et Déontologie – Règles du jeu ou jeu sans règles; Presses Universitaires Francaises, Paris, 1997; Sodré, Nelson Werneck. História da imprensa no Brasil. 2ª ed., Rio de Janeiro: Graal, 1977; Sousa, Jorge Pedro: As Notícias e os seus Efeitos; Minerva Editora,Coimbra, 2000; Veiga Manuel: Cabo Verde insularidade e Literatura, editions Karthala, Paris, 1998; Bem como, um conjunto de leis e projectos de lei ligados a comunicacao social, e algumas entrevistas. Estrutura do trabalho: Esta tese e constituida de: introducao, tres capitulos, conclusao, bibliografia e alguns anexos num total de . Na introducao explica-se a escolha do tema, a sua actualidade, o objecto de investigacao e claro a sua inovacao. No primeiro capitulo estudamos o context do aparecimento dos media nos paises lusofonos nos exemplos de Cabo Verde, Brasil, e Portugal. No Segundo cappitulo, analizamos as leis, os processos de controlo e financiamento dos mass-media e a regulacao dos conteudos dos programas de radio e televisao. No terceiro capitulo, a situacao actual dos meios de comunicacao social e do Jornalismo na CPLP, nele esta tambem exposta a parte practica do trabalho ou seja a analize da relacao do publico com os media e o nivel de confianca do publico na informacao por eles veiculada.

Virtual Guide On Ocular Self-Examination To Support The Self-Care Practice For People With HIV/AIDS

Objetivou-se descrever processo de desenvolvimento da cartilha virtual sobre autoexame ocular para pessoas com HIV/aids. A proposta metodológica seguiu as cinco etapas preconizadas por Falkembach: análise e planejamento, modelagem, implementação, avaliação e distribuição. A adequação da versão impressa para virtual requereu a construção de um vídeo tutorial, agregação de fotos ilustrativas para visualização de possíveis alterações oculares e ferramenta de interatividade com demonstração do resultado do exame ao usuário. Na avaliação inicial do material, foram diagnosticadas falhas no layout. Assim, comandos foram recolocados, unificados, dispostos em local de fácil visualização e foi feita a adequação da linguagem. Considera-se possível promover aproximação do usuário com métodos de prevenção na área da saúde ocular por meio de cartilha virtual, contribuindo para desenvolvimento de habilidades e divulgação do autoexame.


Stoneley wave modeling in heterogeneous porous media with viscous pore fluids

We implemented Biot-type porous wave equations in a pseudo-spectral numerical modeling algorithm for the simulation of Stoneley waves in porous media. Fourier and Chebyshev methods are used to compute the spatial derivatives along the horizontal and vertical directions, respectively. To prevent from overly short time steps due to the small grid spacing at the top and bottom of the model as a consequence of the Chebyshev operator, the mesh is stretched in the vertical direction. As a large benefit, the Chebyshev operator allows for an explicit treatment of interfaces. Boundary conditions can be implemented with a characteristics approach. The characteristic variables are evaluated at zero viscosity. We use this approach to model seismic wave propagation at the interface between a fluid and a porous medium. Each medium is represented by a different mesh and the two meshes are connected through the above described characteristics domain-decomposition method. We show an experiment for sealed pore boundary conditions, where we first compare the numerical solution to an analytical solution. We then show the influence of heterogeneity and viscosity of the pore fluid on the propagation of the Stoneley wave and surface waves in general.

Oligopotent stem cells are distributed throughout the mammalian ocular surface.

The integrity of the cornea, the most anterior part of the eye, is indispensable for vision. Forty-five million individuals worldwide are bilaterally blind and another 135 million have severely impaired vision in both eyes because of loss of corneal transparency; treatments range from local medications to corneal transplants, and more recently to stem cell therapy. The corneal epithelium is a squamous epithelium that is constantly renewing, with a vertical turnover of 7 to 14 days in many mammals. Identification of slow cycling cells (label-retaining cells) in the limbus of the mouse has led to the notion that the limbus is the niche for the stem cells responsible for the long-term renewal of the cornea; hence, the corneal epithelium is supposedly renewed by cells generated at and migrating from the limbus, in marked opposition to other squamous epithelia in which each resident stem cell has in charge a limited area of epithelium. Here we show that the corneal epithelium of the mouse can be serially transplanted, is self-maintained and contains oligopotent stem cells with the capacity to generate goblet cells if provided with a conjunctival environment. Furthermore, the entire ocular surface of the pig, including the cornea, contains oligopotent stem cells (holoclones) with the capacity to generate individual colonies of corneal and conjunctival cells. Therefore, the limbus is not the only niche for corneal stem cells and corneal renewal is not different from other squamous epithelia. We propose a model that unifies our observations with the literature and explains why the limbal region is enriched in stem cells.

On commercial media bias

Within the spokes model of Chen and Riordan (2007) that allowsfor non-localized competition among arbitrary numbers of media outlets, we quantify the effect of concentration of ownership on qualityand bias of media content. A main result shows that too few commercial outlets, or better, too few separate owners of commercial outlets can lead to substantial bias in equilibrium. Increasing the number of outlets (commercial and non-commercial) tends to bring down this bias; but the strongest effect occurs when the number of owners is increased. Allowing for free entry provides lower bounds on fixed costs above which substantial commercial bias occurs in equilibrium.

Allergies aux produits de contraste radiologiques [Allergy to radiographic contrast media].

Allergy to radiographic contrast media Hypersensitivity reactions to radio-contrast media are common in the daily practice. These products are responsible for immediate (< or = 1 hour after administration) and non immediate (> 1 hour after administration) hypersensitivity reactions. A diagnostic work-up by an allergologist with skin tests and in some cases provocation tests is of value in reducing the risk of recurrent hypersensitivity reactions to iodinated contrast media. A careful selection of the patients is required because the incidence of breakthrough reactions is still concerning, even with proper premedication. Practical recommendations are presented in this article. For gadolinium-based contrast agents, data in the literature is not sufficient for suggesting guidelines.

Utilização dos New Media nas Empresas de Comunicação em São Vicente

Este trabalho científico refere-se aos New Media num contexto empresarial, demonstrando essa tecnologia como uma nova forma de comunicar. A delimitação é feita numa perspectiva da ilha de São Vicente, em Cabo Verde, concentrando assim nas empresas de comunicação. Objectiva-se verificar se essas ferramentas da Web 2.0 são utilizadas nesse contexto, se os funcionários são conhecedores dessa tecnologia, qual a prática na sua utilização e se sofrem algum tipo de influência social quando usam os New Media.

Os Media ao Serviço do Imaginário: uma Reflexão Sobre a Rtp Internacional e a Lusofonia

Neste trabalho, não pretendemos analisar detalhadamente a lógica de intervenção relacionada com o desenvolvimento do espaço Lusófono e com a criação da Comunidade dos Países de Língua Portuguesa. Iremos, simplesmente, examinar alguns elementos desta intervenção, nomeadamente no que diz respeito à criação de meios de comunicação especialmente concebidos para dar corpo a esta política. A RTP Internacional e a RTP África serão certamente os canais televisivos que mais visibilidade dão a esta intervenção política, mas o Estado Português tem vindo, também, a promover vários protocolos de cooperação na área da comunicação social. Assim, pretendemos concentrar-nos na lógica de produção e de distribuição de conteúdos televisivos portugueses para as comunidades e países de língua portuguesa e outras iniciativas dos governos portugueses que promovam a exportação e intercâmbio de produtos mediáticos em português. As pertinentes questões relacionadas com a análise da recepção dos produtos culturais não serão abordadas no âmbito deste ensaio.