Morphological, biological and molecular characterization of three strains of Trypanosoma cruzi Chagas, 1909 (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sordida (Stal) 1859 (Hemiptera, Reduviidae) and a domestic cat

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Monitoramento da suscetibilidade de populações de Triatoma sordida Stål, 1859 (Hemiptera: Reduviidae) ao inseticida deltametrina, na região Centro-Oeste do Brasil

INTRODUÇÃO: Populações de Triatoma sordida Stål, 1859 foram investigadas quanto à suscetibilidade à deltametrina. MÉTODOS: Análise por meio de bioensaios por aplicação tópica em 11 populações de T. sordida procedentes dos Estados de Goiás, Mato Grosso e Mato Grosso do Sul. RESULTADOS: As estimativas de DL50 e RR50 demonstraram elevados níveis de suscetibilidade (DL50 < 1 e RR50 < 2). Entretanto, as análises do coeficiente angular da curva dose resposta revelaram que as populações de triatomíneos dos municípios de Firminópolis/GO, Posse/GO, Poxoréu/MT, Douradina/MS e Aparecida do Taboado/MS apresentam maiores probabilidades de evolução de resistência, portanto, mais propícias a tolerar o tratamento com deltametrina. CONCLUSÕES: Detectaram-se pequenas alterações de suscetibilidade e baixos níveis de resistência, porém as alterações temporais de suscetibilidade deverão ser continuamente monitoradas, a fim de nortear adequadamente as ações de controle dos vetores da DC.

Memórias de uma Escola Isolada Rural: estudo de um Livro de Visitas (1928-1948)

Cet article étudie le livre de visites de l'ancienne école mixte de la Fazenda Ponte Alta/Bela Vista, liée au groupe scolaire de la ville de Bariri, dans l'état de São Paulo (SP). À partir des registres de vingt ans d'activités (1928-1948), nous retraçons les exigences des inspecteurs primaires et si ces exigences provenaient (ou non) de professeurs et de la communauté paysanne pour ébaucher un cadre socio-historique de l'enseignement des premières lettres dans la zone rurale de l'intérieur de l'état de São Paulo. Il en résulte que le discours qui défendait l'égalité de chances pour les populations urbaines et rurales négligeait généralement le besoin de promouvoir l'égalité de conditions pour que la communauté rurale puisse profiter des chances qui lui étaient promises.

First case of an infection of the metacercariae of Austrodiplostomum compactum (Lutz, 1928) (Digenea, Diplostomidae) in Hypostomus regani (Ihering, 1905) (Siluriformes: Loricariidae).

Austrodiplostomum compactum (Lutz, 1928) (Digenea, Diplostomidae) was recorded infecting the eyes of a Hypostomus regani (Ihering, 1905) (Siluriformes: Loricariidae). This is the first report of a loricarid fish infected with A. compactum.

Relative growth and sexual maturity of the stone crab Menippe nodifrons Stimpson, 1859 (Brachyura, Xanthoidea) in southeastern Brazil

O objetivo do presente estudo foi determinar o crescimento relativo e a maturidade sexual morfológica de Menippe nodifrons. As coletas foram realizadas na Praia Grande e Tenório, Ubatuba, São Paulo. Os caranguejos foram separados quanto ao sexo e mensurados na região da largura (LC) e comprimento da carapaça, comprimento e altura dos própodos quelares direito e esquerdo, largura do abdome (LA) nas fêmeas e comprimento do gonopódio (CG) nos machos. Obteve-se 399 indivíduos, sendo 195 machos e 204 fêmeas. Os machos atingiram a maturidade sexual com 29,7mm LC e as fêmeas com 31,6mm LC. Para as fêmeas a melhor relação que indicou a muda da puberdade foi LA vs. LC, sendo que o crescimento foi alométrico negativo na fase jovem e alométrico positivo após a muda da puberdade. Para os machos foi CG vs. LC evidenciando crescimento alométrico positivo na fase jovem e isométrico na fase adulta.

Primeiro registro de predação de Poecilia reticulata Peters, 1859 e Phalloceros caudimaculatus (Hensel, 1868) por Noctilio leporinus (Linnaeus, 1758) (Chiroptera, Noctilionidae)

Noctilio leporinus (Chiroptera, Noctilionidae) has adaptations to piscivorous feeding habits, but also consumes crustaceans and insects. Between June 2005 and May 2006, bats were surveyed with mist nets during two evenings per month in the gallery forest of Corrego dos Tenentes, Pindorama, São Paulo State, Brazil. The objectives were to collect fecal samples and identify the fish species that were consumed by N. leporinus in this fragmented forest landscape. We documented two new fish species to its diet, the guppy Poecilia reticulata, Peters, 1859 and the "guaru" Phalloceros caudimaculatus (Hensel, 1868). Both fish species forage near the surface of water for insects and zooplankton, which is an area also utilized by N. leporinus when foraging for insects, crustaceans and fishs.

Metacercariae of Diplostomum compactum Lutz, 1928 (Trematoda, Diplostomidae) in the eyes of acara Geophagus brasiliensis Quoy & Gaimard, 1824 (Teleostei, Cichlidae) from Barra Bonita Reservoir - São Paulo, Brazil

Relata-se a ocorrência de metacercárias de Diplostomum compactum (Trematoda: Diplostomidae) infectando Geophagus brasiliensis (Teleostei: Cichlidae) do reservatório de Barra Bonita, rio Tietê, estado de São Paulo, Brasil. As metacercárias foram coletadas no globo ocular, fixadas em solução de AFA e coradas com carmim. As análises morfológicas e morfométricas de cinco espécimes foram realizadas por meio do sistema computadorizado de analise de imagem. Este foi o primeiro registro da ocorrência desse parasito no reservatório de Barra Bonita e também o primeiro registro em acará Geophagus brasiliensis.

The occurrence of Austrodiplostomum compactum (Lutz, 1928) (Digenea: Diplostomidae) metacercariae in the eyes of loricariid fish (Siluriformes: Osteichthyes: Loricariidae) from Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Prevalência, sazonalidade e intensidade de infecção por Diplostomum (Austrodiplostomum) compactum Lutz, 1928 (Digenea, Diplostomidae), em peixes do reservatório de Volta Grande, Estado de Minas Gerais, Brasil

The present study was conducted in the Aquaculture Station of Hydroeletric Power Station situated in Volta Grande Reservoir, MG, Brazil. Seventy freshwater corvinas, Plagiscion squamosissimus, and 66 tucunarés, Cichla ocellaris were captured bimonthly from April 2000 through April 2001 with net and hook. The helmints were identified as Diplostomun (A.) compactmn which showed the highest prevalence in the corvina's eyes in April 2000 (70%), February 2001 (80%) and April 2001 (60%), while in tucunaré occurred in April 2000 (33.3%), August 2000 (18.2%) and October 2000 (18.2%). Nevertheless, increase in the mean intensity of parasites was related in April (6.6), June (6.0), August (18.5) 2000 and February (5.7), April (4.8) 2001 for corvina and in August (16.0) and October (7.0) 2000 for tucunaré. Corvina's females showed infection during all period, while males did not show the same prevalence in June 2000 and April 2001. On the other hand, tucunare's males were infected in all months while females in August and October 2000. The highest prevalence in corvina was observed in the months which presented elevated water temperature (April, October, December 2000 and April 2001). The number of parasites collected in corvina on February 2000 was higher than the one observed in August 2000. The same was not observed for tucunaré. This work demonstrate corvina's high susceptibility to metacercariae of Diplostomum.

Metacercárias de Diplostomum (Austrodiplostomum) compactum Lutz, 1928 (Digenea, Diplostomidae) em peixes do rio Paraná, Brasil. Prevalência, sazonalidade e intensidade de infecção

This paper aims at evaluating the occurrence of Diplostommn (Anstrodiplostomum compactum (Digenea: Diplostomatidae) in the eyeball of Plagioscion sqnamosissimus (corvina) and Cichla ocellaris (tucunaré), monthly captured in Paraná river, Presidente Epitácio, state of São Paulo, Brazil. From 61 corvinas under analysis, 56 of them contained parasites (92%) with intensity rate of 42.0 host parasites. From an amount of 81 tucunarés, 45 contained parasites (55%) with intensity rate of 9.3 parasites. Corvinas showed prevalence from 71 to 100% with the highest intensity rates (103.3 and 106.9) from February to March, 2001. Comparatively, the tucunaré showed the highest prevalence from November 2000 (90%) to February 2001 (80%). The tucunaré highest intensity rates occurred on September, 2000 (12.7), December, 2000 (12.2) and February, 2001 (16.1). Metacercariae showed a hollow womb body and a posterior region of a sinuous conic protuberance, an oral sucker smaller than the acetabulae, a simple acetabulae located in the pre-equatorial region of helminto. A short pre-pharynx followed by a muscular pharynx, genital pore next to acetubulae. Corvina metacercariae presents 1.434,0 μm (880 to 1.840) long by 611.2 ± 93.4 μm (400 to 792) wide. Tucunaré metacercariae, 1.462.4 μm (960 to 2.480) by 710.8 μm (560 to 960). Authors confirmed corvina great susceptibility to Diplomastum and the relationship between the parasitism and the aquatic and climatic parameters.

Estudo biológico e biométrico dos estádios ninfais de Rhodnius prolixus Stäl, 1859 (Hemiptera, Reduviidae) sob condições laboratoriais

Eggs and nymphs originated from couples of Rhodnius prolixus obtained from nymphs of the 5th instar were used for biological cycle and biometric studies. The following biological cycle parameters were determined under a temperature of 28°C and relative humidity, varying between 52 - 94% : medium period of incubation: 13.01 days; rate of eggs eclosion: 77.6%; medium period of development of the 1st, 2nd, 3rd, 4 th, 5th instar nymphs : 19.33, 19.09, 20.38, 24.37, 38.14 days, respectively; percentual of deaths in the nymph instar: 26.70, 14.00, 18.26, 17.02, 35.47% respectively; percentual of changes per instar nymphs; 73.30, 86.03, 81.73, 82.97, 64.52%, respectively. Biometric measurements performed, showed that in all the instars the abdomen is the largest segment. In the four first instars, the head is larger than the thorax. In the fifth instar, the head and thorax present are about the same size.

The megalopa stage of Portunus spinimanus Latreille, 1819 and Portunus gibbesii (Stimpson, 1859) (Decapoda, Brachyura, Portunidae) from the southeastern Atlantic coast of the United States

The identification of megalopae from plankton samples is difficult, because this larval stage is the least well known among crab larvae, unknown in some species and poorly described in others. Wild megalopa specimens of some swimming crabs (family Portunidae Rafinesque, 1815) were captured alive from neuston samples obtained during summer surveys near the coast of Charleston, South Carolina (U.S.A). For identification purposes, larvae were reared to the 8th juvenile instar. After reaching the 5th juvenile instar, the juvenile crabs exhibited morphological features suitable for identification to the species level. The specimens belonged to two species of Portunidae, Portunus spinimanus Latreille, 1819 and P. gibbesii (Stimpson, 1859). Their megalopae were described in detail and compared to other portunid megalopae known from the southeastern Atlantic coast of the U.S.A. Species-specific characters of portunid megalopae are the number of carpal spines on the chelipeds, the relative size of the sternal spines (7th sternite), the number of antennal flagellum segments, and the setation of mouthparts. Copyright © 2007 Magnolia Press.

Importancia de los sustratos biogénicos para el cangrejo de piedra Menippe nodifrons Stimpson, 1859 (Brachyura: Eriphioidea)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

A presença feminina na ação educacional presbiteriana no Brasil do século XIX (1859-1899)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)