Defence chemistry modulation by light and temperature (results in 4 excel files, zipped)

The goals of this study were (1) to investigate whether Fucus vesiculosus regulates the production of its antifouling defence chemicals against microfoulers in response to light limitation and temperature shifts and (2) to investigate if different surface concentrations of defence compounds shape epibacterial communities. F. vesiculosus was incubated in indoor mesocosms at five different temperature conditions (5 to 25°C) and in outdoor mesocosms under six differently reduced sunlight conditions (0 to 100%), respectively. Algal surface concentrations of previously identified antifouling compounds - dimethylsulphopropionate (DMSP), fucoxanthin and proline - were determined and the bacterial community composition was characterized by in-depth sequencing of the 16S-rRNA gene. Altogether, the effect of different treatment levels upon defence compound concentrations was limited. Under all conditions DMSP alone appeared to be sufficiently concentrated to warrant for at least a partial inhibitory action against epibiotic bacteria of F. vesiculosus. In contrast, proline and fucoxanthin rarely reached the necessary concentration ranges for self-contained inhibition. Nonetheless, in both experiments along with the direct influence of temperature and light, all three compounds apparently affected (and thereby shaped) the overall bacterial community composition associated with F. vesiculosus since tendencies for insensitivity towards all three compounds were observed among bacterial taxa that typically dominate those communities. Given that the concentrations of at least one of the compounds (in most cases DMSP) were always high enough to inhibit bacterial settlement, we conclude that the capacity of F. vesiculosus for such defence will hardly be compromised by shading or warming to temperatures up to 25°C.

Diseño e implementación de un controlador de ratón gestual para Microsoft Windows con Kinect - Touchless Gesture User Interface

El dispositivo Microsoft Kinect for Windows y similares, han introducido en el mundo del PC una nueva forma de interacción denominada “Touchless Gesture User Interface” o TGUI (Interfaz de Usuario por Gestos sin Contacto) [Gentile et al. 2011]. Se trata de una tecnología novedosa en proceso de evolución. La tecnología de Kinect detecta la presencia de un usuario y monitoriza la posición en el espacio de sus articulaciones principales. Esta información permite desarrollar aplicaciones que posibiliten interactuar al usuario con una computadora mediante gestos y sin la necesidad de estar en contacto con periférico alguno. Desde la invención del periférico ratón en los años 60, resulta curioso que con la frenética evolución que ha experimentado el mundo de la informática en todos estos años, este dispositivo no haya sufrido cambios significativos o no haya sido incluso sustituido por otro periférico. En este proyecto se ha abordado el reto de desarrollar un controlador de ratón gestual para Windows utilizando Microsoft Kinect, de tal forma que se sustituya el uso del típico ratón y sea el propio usuario el que actúe como controlador mediante gestos y movimientos de sus manos. El resultado es llamativo y aporta numerosas mejoras y novedades frente a aplicaciones similares, aunque deja en evidencia algunas de las limitaciones de la tecnología implementada por Kinect a día de hoy. Es de esperar que cuando evolucione su tecnología, su uso se convierta en cotidiano.