994 resultados para Her-2 Neu Oncogene


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Preface signed by author's son: W.W. Tulloch.

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Vols.1-7 edited by J. Stevenson; v.8-11, by A. J. Crosby; v.12-17, by A. J. Butler (v.17 with S. C. Lomas); v.18-21, by S. C. Lomas (v.18, pt.2-4 with A. B. Hinds); v.22- by R. B. Wernham.

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v. 1. From the first colonization of the valley to the visit of the patriarch Abram.--v. 2. From the visit of Abram to the exodus.

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[im Auftrag des Reichs-Versicherungsamts bearbeitet von Dr. Zacher. Fortgeführt unter Mitwirkung von L. Laß und G.A. Klein]

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The serine protease inhibitor SerpinB2 (PAI-2), a major product of differentiating squamous epithelial cells, has recently been shown to bind and protect the retinoblastoma protein (Rb) from degradation. In human papillomavirus type 18 (HPV-18) -transformed epithelial cells the expression of the E6 and E7 oncoproteins is controlled by the HPV-18 upstream regulatory region (URR). Here we illustrate that PAI-2 expression in the HPV-18-transformed cervical carcinoma line HeLa resulted in the restoration of Rb expression, which led to the functional silencing of transcription from the HPV-18 URR. This caused loss of E7 protein expression and restoration of multiple E6- and E7-targeted host proteins, including p53, c-Myc, and c-Jun. Rb expression emerged as sufficient for the transcriptional repression of the URR, with repression mediated via the C/EB beta-YY1 binding site (URR 7709 to 7719). In contrast to HeLa cells, where the C/EBP beta-YY1 dimer binds this site, in PAI-2- and/or Rb-expressing cells the site was occupied by the dominant-negative C/EBP beta isoform liver-enriched transcriptional inhibitory protein (LIP). PAI-2 expression thus has a potent suppressive effect on HPV-18 oncogene transcription mediated by Rb and LIP, a finding with potential implications for prognosis and treatment of HPV-transformed lesions.