The SNAP45 subunit of the small nuclear RNA (snRNA) activating protein complex is required for RNA polymerase II and III snRNA gene transcription and interacts with the TATA box binding protein.

The RNA polymerase II and III small nuclear RNA (snRNA) promoters contain a common basal promoter element, the proximal sequence element (PSE). The PSE binds a multisubunit complex we refer to as the snRNA activating protein complex (SNAPc). At least four polypeptides are visible in purified SNAPc preparations, which migrate with apparent molecular masses of 43, 45, 50, and 190 kDa on SDS/polyacrylamide gels. In addition, purified preparations of SNAPc contain variable amounts of TATA box binding protein (TBP). An important question is whether the PSEs of RNA polymerase II and III snRNA promoters recruit the exact same SNAP complex or slightly different versions of SNAPc, differing, for example, by the presence or absence of a subunit. To address this question, we are isolating cDNAs encoding different subunits of SNAPc. We have previously isolated the cDNA encoding the 43-kDa subunit SNAP43. We now report the isolation of the cDNA that encodes the p45 polypeptide. Antibodies directed against p45 retard the mobility of the SNAPc-PSE complex in an electrophoretic mobility shift assay, indicating that p45 is indeed part of SNAPc. We therefore refer to this protein as SNAP45. SNAP45 is exceptionally proline-rich, interacts strongly with TBP, and, like SNAP43, is required for both RNA polymerase II and III transcription of snRNA genes.

List of students, 1789

Small notebook with brown paper covers containing handwritten lists of the members of the Harvard Classes of 1790, 1791, 1792, and 1793 (through Charles Jackson) with unidentified annotations next to some names of a, c, o, s, t, and x.

Interleaved almanac, 1746

The 1746 diary is interleaved in Poor Richard's, An almanack for the year of Christ 1746. The thin paper-covered book holds brief notes about Holyoke’s daily life, written on blank pages bound with the almanac. More consistently filled out, the diary continues information about Holyoke’s senior year at Harvard, and his appointment as a schoolteacher in Lexington, Massachusetts. The September and October entries reference local military movements of King George’s War. The diary includes regular entries on individuals who preached and lectured at Harvard. Holyoke mentions an earthquake in February, and in March he notes that Harvard Professor John Winthrop "had a lecture: Solving ye Aurora Bor[ealis]."

Letter from Rufus King to William Tudor,

One letter regarding a meeting between the elder William Tudor and the King of England at the Court of Saint James.

Luther's reply to King Henry VIII, now first Englished after the lapse of four centuries,

Mode of access: Internet.

The Voyage of Admiral George Carlton, in search of loyalty. : A poetic epistle.

Page [2] is blank.

Fair Em, the miller's daughter of Manchester : with the love of William the Conqueror /

Imprint fictitious. Printed by the editor for private circulation. Cf. Jaggard, William. Shakespeare bibliography, 1911, page 552.

A scripture catechism, for the instruction of children and servants /

Includes publisher's advertisement on lower cover.

The armies of the first French Republic and the rise of the marshals of Napoleon I ...

Preface signed: Charles F. Phipps.

Bismarck, the man and the statesman : being the reflections and reminiscences of Otto, prince von Bismarck,

Translation of the author's Gedanken und erinnerungen, Stuttgart, 1898.

The life of Louis XI, the rebel dauphin and the statesman king, from his original letters and other documents;

"Printed in Great Britain."

Memoirs of Elizabeth Stuart, queen of Bohemia, daughter of King James the First. Including sketches of the state of society in Holland and Germany, in the 17th century.

Mode of access: Internet.

Lives of men of letters & science who flourished in the time of George III.

Vol. 2 has imprint: London, H. Colburn.

The reform act, 1832; the correspondence of the late Earl Grey with His Majesty King William IV. and with Sir Herbert Taylor, from Nov. 1830 to June 1832;

Mode of access: Internet.

The life and reign of William the Fourth.

Written in collaboration with John Watkins. cf. Dict. nat. biog.