Rotações de Culturas e Afolhamentos

A rotação de culturas é uma prática agronómica importante em todos os sistemas de agricultura. A alternância de culturas de espécies com características distintas ao nível morfológico (sistema radical), ciclo vegetativo (épocas distintas de sementeira e colheita) e ao nível da sua resistência a pragas e doenças, contribui para o aumento da melhoria das características físicas, químicas e biológicas dos solos. A rotação de culturas pode melhorar a estrutura do solo, quer pela introdução de matéria orgânica, quer pela porosidade biológica criada pelas raízes das culturas. O aumento da porosidade biológica conduzirá a uma maior infiltração da água no solo com consequência na redução do escoamento superficial e portanto, da erosão hídrica. O acréscimo da porosidade biológica no solo pelas raízes é de extrema importância, principalmente em sistemas de mobilização nula (sementeira directa). A utilização de plantas leguminosas, como por exemplo a Vicia sativa L. (vicia ou ervilhaca) a Lupinus luteus L.(tremocilha), o Cicer arietinum L. (grão-de-bico) a Pisum sativum L. (ervilha), etc., na rotação, favorecerá o incremento de azoto no solo, o qual será favorável ao crescimento das gramíneas com redução dos seus custos de produção. Outro aspecto extremamente importante da rotação de culturas prende-se com a melhor distribuição do parque de máquinas e da mão-de-obra ao longo do ano, fazendo-se alternar culturas com épocas de sementeira e de colheita diferentes, como por exemplo o Helianthus annuus L. (girassol) que é uma cultura de primavera-verão, o trigo mole (Triticum aestivum L.) e a cevada dística (Hordeum distichum L.) que são culturas de outono-inverno, etc.

Resistência à penetração e atributos químicos em um latossolo do Piauí sob monocultivos e consórcio de gramíneas irrigados.

Embora os benefícios proporcionados pela irrigação às plantas sejam sistematicamente conhecidos, há no Brasil, principalmente nos perímetros irrigados do Estado do Piauí, carência de estudos sobre o efeito dessa prática nos atributos físico, químico e biológico do solo. Com base neste enfoque o objetivo deste trabalho foi avaliar a influência de lâminas de irrigação em sistemas de produção sobre a resistência a penetração da raiz (RP) e atributos químicos de um Latossolo Amarelo Distrófico, visando a implantação do sistema de integração lavoura-pecuária no Perímetro Irrigado Gurguéia, PI.

Seleção de gramíneas forrageiras com base na resistência às cigarrinhas- das-pastagens

2015

Avaliação de gramíneas na microrregião do Médio-Mearim, MA.

Em Bacabal, MA, Foram avaliados o rendimento forrageiro, a percentagem de folhas e de FDN e FDA de Brachiaria brizantha cv. Marandu. Brachiaria decumbens cv. Basilisk, Panicum maximum cvs. Massai, Mombaça, Tanzânia e Tobiatã. O plantio foi realizado no inicio das chuvas (janeiro) de 1999 e os cortes para avaliação, em número de oito, realizaram-se de abril de 1999 até julho de 2000. Foi usado o delineamento inteiramente casualizado com parcelas de 3 x 5 m e três repetições.

Bactérias assimbióticas fixadoras de N na rizosfera de gramíneas forrageiras.

1966

Como água e temperatura afetam a germinação de plantas daninhas poáceas de verão?

2016

Efeito do calcário em latossolo amarelo álico na produção de culturas anuais.

Avaliação da resposta de culturas anuais a aplicação de calcário. Importância do calcário como neutralizador da acidez do solo.

Avaliação do desempenho de gramíneas para paisagismo submetidas à diferentes níveis de fertilidades de solo.

2016

Let's talk about failures : why was the game for children not a success?

A significant proportion of research in the field of human-computer interaction has been devoted to game design. Yet, a multitude of good ideas and enthusiastic game design initiatives exist, where the games never see the light of day. Unfortunately, the causes of these failures remain often unexplored and unpublished. The challenges faced by researchers and practitioners are particularly complex when designing games for special target groups, such as children, or for a serious purpose. The HCI community would benefit from a discussion on these issues in order to avoid researchers and practitioners to repeat mistakes. We want to learn from projects that started with a promising idea, but failed or faced severe challenges. This workshop will be the first at CHI focusing on 'failed game projects'. In particular, workshop participants are encouraged to discuss issues that typically received little attention in publications and hereby contribute to the discussion on failures in the design, development and evaluation of games for and or with children. As a result, the community will benefit from these insights and lessons-learned, which will enhance the design of future (serious) games with/for children.

More naturalness, less control: The effect of natural mapping on the co-located player experience

In the past years, there has been a surge in game controllers that allow players to play in a more physical, more natural way. In this paper we present an experimental study of the effect of gaming using these naturally mapped controllers on the player experience in a social setting. Results support the hypothesis that more naturally mapped controllers augment spatial presence. Furthermore, the results suggest that gaming with more naturally mapped controllers augment social presence for female players, but not for male players. However, gaming via naturally mapped controllers decreases perceived control and actual performance. Hence, users with high performance expectations might not benefit from gaming via naturally mapped controllers.

Equine herpesvirus infections in yearlings in South-East Queensland.

Twelve nasal swabs were collected from yearling horses with respiratory distress and tested for equid herpesvirus 1 (EHV-1) and equid herpesvirus 4 (EHV-4) by real-time PCR targeting the glycoprotein B gene. All samples were negative for EHV-1; however, 3 were positive for EHV-4. When these samples were tested for EHV-2 and EHV-5 by PCR, all samples were negative for EHV-2 and 11 were positive for EHV-5. All three samples that were positive for EHV-4 were also positive for EHV-5. These three samples gave a limited CPE in ED cells reminiscent of EHV-4 CPE. EHV-4 CPE was obvious after 3 days and was characterised by syncytia. None of the samples produced cytopathic effect (CPE) on African green monkey kidney (Vero) cells or hamster kidney (BSR) cells. Four of the samples, which were positive in the EHV-5 PCR, produced CPE on rabbit kidney (RK13) cells and equine dermis (ED) cells. EHV-5 CPE on both cell lines was slow and was apparent after four 7-day passages. On RK13 cells, the CPE was characteristic of equid herpesvirus, with the formation of syncytia. However, in ED cells, the CPE was characterised by ring-shaped syncytia. For the first time, a case of equine respiratory disease involving dual infection with EHV-4 and EHV-5 has been reported in Queensland (Australia). This was shown by simultaneously isolating EHV-4 and EHV-5 from clinical samples. EHV5 was recovered from all samples except one, suggesting that EHV5 was more prevalent in young horses than EHV2.

Dachau concentration camp - a memoir May to December of 1938

This memoir provides a detailed description of daily life and misery in the concentration camp Dachau. The first eight chapters are missing which would cover Felix Klinen's life in Vienna. The existing memoir then starts with his deportation to Dachau, and ends shortly before his transfer to Buchenwald concentration camp, covering the time from May to December of 1938. Translated from the German by Sanda Vero

Inhibition of replication of rinderpest virus by 5-fluorouracil

5-Fluorouracil (5FU), an analogue of uracil, was found to inhibit the production of infectious particles of rinderpest virus (RPV) in Vero cells (African green monkey kidney cells) by 99%, at a concentration of 1 μg/ml. The levels of individual mRNA specific for five of the virus genes were also reduced drastically, while the level of mRNA for a cellular housekeeping gene—glyceraldehyde-3-phosphate dehydrogenase (GAPDH)—was unaltered by fluorouracil treatment of infected cells. Both virus RNA and protein synthesis showed inhibition in a dose-dependent manner. The virions which budded out of 5-fluorouracil-treated cells also contained reduced amounts of virus proteins compared with virus particles from untreated cells.

Host factor Ebp1 inhibits rinderpest virus transcription in vivo

ErbB3 binding protein Ebp1 has been shown to downregulate ErbB3 receptor-mediated signaling to inhibit cell proliferation. Rinderpest virus belongs to the family Paramyxoviridae and is characterized by the presence of a non-segmented negative-sense RNA genome. In this work, we show that rinderpest virus infection of Vero cells leads to the down-regulation of the host factor Ebp1, at both the mRNA and protein levels. Ebp1 protein has been shown to co-localize with viral inclusion bodies in infected cells, and it is packaged into virions, presumably through its interaction with the N protein or the N-RNA itself. Overexpression of Ebp1 inhibits viral transcription and multiplication in infected cells, suggesting that a mutual antagonism operates between host factor Ebp1 and the virus.

Hantavirus glycoprotein GN in virion assembly and regulation of interferon response

Hantaviruses have a tri-segmented negative-stranded RNA genome. The S segment encodes the nucleocapsid protein (N), M segment two glycoproteins, Gn and Gc, and the L segment the RNA polymerase. Gn and Gc are co-translationally cleaved from a precursor and targeted to the cis-Golgi compartment. The Gn glycoprotein consists of an external domain, a transmembrane domain and a C-terminal cytoplasmic domain. In addition, the S segment of some hantaviruses, including Tula and Puumala virus, have an open reading frame (ORF) encoding a nonstructural potein NSs that can function as a weak interferon antagonist. The mechanisms of hantavirus-induced pathogenesis are not fully understood but it is known that both hemorrhagic fever with renal syndrome (HFRS) and hantavirus (cardio) pulmonary syndrome (HCPS) share various features such as increased capillary permeability, thrombocytopenia and upregulation of TNF-. Several hantaviruses have been reported to induce programmed cell death (apoptosis), such as TULV-infected Vero E6 cells which is known to be defective in interferon signaling. Recently reports describing properties of the hantavirus Gn cytoplasmic tail (Gn-CT) have appeared. The Gn-CT of hantaviruses contains animmunoreceptor tyrosine-based activation motif (ITAM) which directs receptor signaling in immune and endothelial cells; and contain highly conserved classical zinc finger domains which may have a role in the interaction with N protein. More functions of Gn protein have been discovered, but much still remains unknown. Our aim was to study the functions of Gn protein from several aspects: synthesis, degradation and interaction with N protein. Gn protein was reported to inhibit interferon induction and amplication. For this reason, we also carried out projects studying the mechanisms of IFN induction and evasion by hantavirus. We first showed degradation and aggresome formation of the Gn-CT of the apathogenic TULV. It was reported earlier that the degradation of Gn-CT is related to the pathogenicity of hantavirus. We found that the Gn-CT of the apathogenic hantaviruses (TULV, Prospect Hill virus) was degraded through the ubiquitin-proteasome pathway, and TULV Gn-CT formed aggresomes upon treatment with proteasomal inhibitor. Thus the results suggest that degradation and aggregation of the Gn-CT may be a general property of most hantaviruses, unrelated to pathogenicity. Second, we investigated the interaction of TULV N protein and the TULV Gn-CT. The Gn protein is located on the Golgi membrane and its interaction with N protein has been thought to determine the cargo of the hantaviral ribonucleoprotein which is an important step in virus assembly, but direct evidence has not been reported. We found that TULV Gn-CT fused with GST tag expressed in bacteria can pull-down the N protein expressed in mammalian cells; a mutagenesis assay was carried out, in which we found that the zinc finger motif in Gn-CT and RNA-binding motif in N protein are indispensable for the interaction. For the study of mechanisms of IFN induction and evasion by Old World hantavirus, we found that Old World hantaviruses do not produce detectable amounts of dsRNA in infected cells and the 5 -termini of their genomic RNAs are monophosphorylated. DsRNA and tri-phosphorylated RNA are considered to be critical activators of innate immnity response by interacting with PRRs (pattern recognition receptors). We examined systematically the 5´-termini of hantavirus genomic RNAs and the dsRNA production by different species of hantaviruses. We found that no detectable dsRNA was produced in cells infected by the two groups of the old world hantaviruses: Seoul, Dobrava, Saaremaa, Puumala and Tula. We also found that the genomic RNAs of these Old World hantaviruses carry 5´-monophosphate and are unable to trigger interferon induction. The antiviral response is mainly mediated by alpha/beta interferon. Recently the glycoproteins of the pathogenic hantaviruses Sin Nombre and New York-1 viruses were reported to regulate cellular interferon. We found that Gn-CT can inhibit the induction of IFN activation through Toll-like receptor (TLR) and retinoic acid-inducible gene I-like RNA helicases (RLH) pathway and that the inhibition target lies at the level of TANK-binding kinase 1 (TBK-1)/ IKK epislon complex and myeloid differentiation primary response gene (88) (MyD88) / interferon regulatory factor 7 (IRF-7) complex.