Evidence of cryptic and pseudocryptic speciation in the Paracalanus parvus species complex (Crustacea, Copepoda, Calanoida), supplementary material

Introduction Many marine planktonic crustaceans such as copepods have been considered as widespread organisms. However, the growing evidence for cryptic and pseudo-cryptic speciation has emphasized the need of re-evaluating the status of copepod species complexes in molecular and morphological studies to get a clearer picture about pelagic marine species as evolutionary units and their distributions. This study analyses the molecular diversity of the ecologically important Paracalanus parvus species complex. Its seven currently recognized species are abundant and also often dominant in marine coastal regions worldwide from temperate to tropical oceans. Results COI and Cytochrome b sequences of 160 specimens of the Paracalanus parvus complex from all oceans were obtained. Furthermore, 42 COI sequences from GenBank were added for the genetic analyses. Thirteen distinct molecular operational taxonomic units (MOTU) and two single sequences were revealed with cladistic analyses (Maximum Likelihood, Bayesian Inference), of which seven were identical with results from species delimitation methods (barcode gaps, ABDG, GMYC, Rosenberg's P(AB)). In total, 10 to 12 putative species were detected and could be placed in three categories: (1) temperate geographically isolated, (2) warm-temperate to tropical wider spread and (3) circumglobal warm-water species. Conclusions The present study provides evidence of cryptic or pseudocryptic speciation in the Paracalanus parvus complex. One major insight is that the species Paracalanus parvus s.s. is not panmictic, but may be restricted in its distribution to the northeastern Atlantic.

Distribution of euphausiids (Crustacea) at the Great Meteor seamount, Atlantic Ocean

In the course of the voyages 9a and 9c (1967) and 19 (1970) of the RV "Meteor" samples of plankton and neuston have been taken in the area of the Great Meteor Seamount. The euphausiids of this material have been examined quantitatively as well as qualitatively in order to study the influence of the Great and Small Meteor Seamount on a vertically migrating group of plankton. 20 species could be identified. All stem from the surrounding deep water and belong to the tropical and subtropical fauna. On the plateau of the Great Meteor Seamount no indigenous species have been encountered and also the typical neritic species from the west coast off Africa are lacking. As for the euphausiids no relationships exist between the Great Meteor Seamount and the shelf area of West Africa. The dominant species around the Meteor Seamount were Euphausia brevii, Stylocheiron suhmii, E. hemigibba, S. longicorne and Thysanopoda subaequalis. Using the index of diversity (Simpson) distinct differences in the composition of species could be shown to exist between the plateau area of the Meteor Seamount and the surrounding sea. On the plateau of the Great Meteor Seamount the number of species was only 7, E. brevis and S. suhmii dominated. None of the species occurred in great numbers and none is adapted to the specific environmental conditions of the plateau of the Meteor Seamount. The fauna of the plateau is a depauperate one as compared with that of the surrounding sea. This can be explained by the fact that adult euphausiids require for their existence greater water depths than are found above the plateau of the Meteor Seamount.