Cytogenetic studies in six species of Scinax (Anura, Hylidae) clade Scinax ruber from northern and northeastern Brazil

Scinax species are still underrepresented in cytogenetic studies, mainly with respect to populations from northeastern and northern Brazil. In this study, we provide new chromosomal information on Scinax boesemani, S. camposseabrai, S. garbei, S. pachycrus, S. trilineatus and S. x-signatus, all belonging to clade S. ruber. They were collected at two locations in the Caatinga biome (northeastern Brazil) and at one in the Amazon (northern Brazil) biomes. Chromosomes were analyzed by conventional staining, C-banding, Ag-NOR staining, and fluorochrome staining. All species shared a modal diploid value of 2n = 24 and fundamental arm number (FN) of 48. Moreover, both chromosomal size and morphology were similar to other species in this Scinax clade. C-banding revealed centromeric heterochromatin in all species, along with terminal species-specific C-bands in some species. Active nucleolar organizer regions (Ag-NORs) were identified at 11q in most species, except for S. boesemani and S. garbei (Ag-NORs at interstitial region of 8q). Differing from most anurans, GC-rich regions were not restricted to NORs, but also coincident with some centromeric and terminal C-bands. These data contribute to the cytotaxonomy of Scinax by providing chromosomal markers and demonstrating the occurrence of microstructural rearrangements and inversions on chromosomal evolution of Scinax.

Karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed

Domestic buffaloes are divided into two group based on cytogenetic characteristics and habitats: the “river buffaloes” with 2n = 50 and the “swamp buffaloes”, 2n = 48. Nevertheless, their hybrids are viable, fertile and identified by a 2n = 49. In order to have a better characterization of these different cytotypes of buffaloes, and considering that NOR-bearing chromosomes are involved in the rearrangements responsible for the karyotypic differences, we applied silver staining (Ag-NOR) and performed fluorescent in situ hybridization (FISH) experiments using 18S rDNA as probe. Metaphases were obtained through blood lymphocyte culture of 21 individuals, including river, swamp and hybrid cytotypes. Ag-NOR staining revealed active NORs on six chromosome pairs (3p, 4p, 6, 21, 23, 24) in the river buffaloes, whereas the swamp buffaloes presented only five NOR-bearing pairs (4p, 6, 20, 22, 23). The F1 crossbreed had 11 chromosomes with active NORs, indicating expression of both parental chromosomes. FISH analysis confirmed the numerical divergence identified with Ag-NOR. This result is explained by the loss of the NOR located on chromosome 4p in the river buffalo, which is involved in the tandem fusion with chromosome 9 in this subspecies. A comparison with the ancestral cattle karyotype suggests that the NOR found on the 3p of the river buffalo may have originated from a duplication of ribosomal genes, resulting in the formation of new NOR sites in this subspecies.

Substâncias húmicas aquáticas do Rio Negro-AM: influência da sazonalidade nas características estruturais, distribuição de carbono e capacidade de complexação por íons Hg(II) em função do tamanho molecular

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Alterações cromossômicas estruturais e evolução caritotípica em Astyanax fasciatus (Teleostei, Characiformes, Characidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A rare non-Robertsonian translocation involving chromosomes 15 and 21

CONTEXTO:Translocações robertsonianas (TR) estão entre os rearranjos estruturais balanceados mais comuns em humanos e compreendem a fusão da cromatina completa do braço longo de dois cromossomos acrocêntricos. No entanto, são raras as translocações não Robertsonianas envolvendo esses cromossomos.RELATO DE CASO:Nós descrevemos uma translocação não balanceada de novo envolvendo os cromossomos 15 e 21. A recém-nascida era filha de uma mãe de 29 anos e de um pai de 42 anos, casal não consanguíneo. Os achados clínicos levaram ao diagnóstico de síndrome de Down (SD) com defeitos cardíacos congênitos graves (persistência do canal arterial e defeito do septo atrioventricular completo), além de baixos comprimento e peso ao nascimento (< 5o e < 10o percentil em curvas de medidas específicas para SD, respectivamente). A análise citogenética convencional revelou o cariótipo 46,XX,der(15)(15pter→15q26.2

Comparative cytogenetics in Astyanax (Characiformes: Characidae) with focus on the cytotaxonomy of the group

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

A dimensão pulsional do sensível: elaborações acerca da percepção em Renaud Barbaras

In this paper, we analyze the theoretical work of Renaud Barbaras, departing from his proposal to highlight and develop the potential of Husserl's theory of perception, based on the doctrine of perceptive donation according to profiles. We mainly focused on his work entitled Desire and distance: introduction to a phenomenology of perception. We underline the description, operated by the author, about the vital movement as desire and the characterization of the subject of the perception as living. In this condition, the formulation about the priority of a dimension emerges, qualified by Barbaras as pulsion towards the objectifying dimension of classical epistemology in the subject-object relation. We indicate that, according to the author, these rearrangements entail the need to consider the body, perception and movement based on the category of life.

Transmissable Dog Cancer Genome Reveals the Origin and History of an Ancient Cell Lineage

Canine transmissible venereal tumor (CTVT) is the oldest known somatic cell lineage. It is a transmissible cancer that propagates naturally in dogs. We sequenced the genomes of two CTVT tumors and found that CTVT has acquired 1.9 million somatic substitution mutations and bears evidence of exposure to ultraviolet light. CTVT is remarkably stable and lacks subclonal heterogeneity despite thousands of rearrangements, copy-number changes, and retrotransposon insertions. More than 10,000 genes carry nonsynonymous variants, and 646 genes have been lost. CTVT first arose in a dog with low genomic heterozygosity that may have lived about 11,000 years ago. The cancer spawned by this individual dispersed across continents about 500 years ago. Our results provide a genetic identikit of an ancient dog and demonstrate the robustness of mammalian somatic cells to survive for millennia despite a massive mutation burden.

Chromosomal organization and evolutionary history of Mariner transposable elements in Scarabaeinae coleopterans

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Male Gonadal Differentiation and the Paedomorphic Evolution of the Testis in Teleostei

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

High Diversity in CMA(3)/DAPI-Banding Patterns in Heteropterans

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

The conservation of number and location of 18S sites indicates the relative stability of rDNA in species of Pentatomomorpha (Heteroptera)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Origin and distribution of AT-rich repetitive DNA families in Triatoma infestans (Heteroptera)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic Spacers

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A importância do conhecimento e da inovação na região metropolitana de Campinas-SP: o caso do condomínio empresarial Praça Capital

The present work is to analyze the importance of exchanging knowledge and technological innovation in Business Condominium Square Capital installed in the Metropolitan Region of Campinas (SP). The condominium corporate Capital Square is the new result of the productive rearrangements that allow the reproduction of capital. This new production space, endowed with infrastructure and sophisticated logistics is able to enable the development of companies installed there in addition to promoting the advancement of technological innovation in the region because of its relations with laboratories, universities and research centers. The research aims to contribute to the understanding of this new form of organization of space activities corresponding to the logic of exchange of knowledge and technological innovation. The logical location of innovation services have similar aspects to economic activities in general, which is distinguished due to the intangible nature of services of high added value through the strategic role of information, the nature of services and because there are services whose logic operation is beyond the economic, but also scientific. The project includes the study in an integrated environment, which allows to evaluate, under various aspects, the importance of established companies and infrastructure available that enable the advancement of skills and knowledge exchange in Capital Square Condo