Characteristics of Yersinia pseudotuberculosis isolated from animals in Brazil

Strains (105) of Yersinia pseudotuberculosis isolated in Brazil between 1982 and 1990 were bio-serotyped. They were also studied for plasmid profile, autoagglutination and calcium dependence at 37 degrees C, Congo red uptake, pyrazinamidase activity, esculin hydrolysis, salicin fermentation and drug sensitivity: 95.24% were biotype 2, serogroup O:3; 2.86% were biotype 1, serogroup O:1; and 1.90% were biotype 2, non-agglutinable. Plasmids were found in 77.14% of the strains (one in each strain). There was total correlation between the presence of the virulence plasmid and autoagglutination, calcium dependence at 37 degrees C and Congo red uptake. The esculin, salicin and pyrazinamidase tests were not efficient in differentiating pathogenic from non-pathogenic Y. pseudotuberculosis isolates. All strains were highly sensitive to the drugs used. These results indicate that Y. pseudotuberculosis is a potential pathogen for humans in Brazil, especially because the bio-serogroups detected among animals are those most frequently associated with human diseases.

Selective suprascapular and axillary nerve block provides adequate analgesia and minimal motor block. Comparison with interscalene block

Shoulder arthroscopic surgeries evolve with intense postoperative pain. Several analgesic techniques have been advocated. The aim of this study was to compare suprascapular and axillary nerve blocks in shoulder arthroscopy using the interscalene approach to brachial plexus blockade. According to the technique used, sixty-eight patients were allocated into two groups: interscalene group (IG, n=34) and selective group (SG, n=34), with neurostimulation approach used for both techniques. After appropriate motor response, IG received 30 mL of 0.33% levobupivacaine in 50% enantiomeric excess with adrenalin 1:200,000. After motor response of suprascapular and axillary nerves, SG received 15 mL of the same substance on each nerve. General anesthesia was then administered. Variables assessed were time to perform the blocks, analgesia, opioid consumption, motor block, cardiovascular stability, patient satisfaction and acceptability. Time for interscalene blockade was significantly shorter than for selective blockade. Analgesia was significantly higher in the immediate postoperative period in IG and in the late postoperative period in SG. Morphine consumption was significantly higher in the first hour in SG. Motor block was significantly lower in SG. There was no difference between groups regarding cardiocirculatory stability and patient satisfaction and acceptability. Failure occurred in IG (1) and SG (2). Both techniques are safe, effective, and with the same degree of satisfaction and acceptability. The selective blockade of both nerves showed satisfactory analgesia, with the advantage of providing motor block restricted to the shoulder.

TF-Test Modified: new diagnostic dool for human enteroparasitosis

Intestinal parasitosis is highly prevalent worldwide, being among the main causes of illness and death in humans. Currently, laboratory diagnosis of the intestinal parasites is accomplished through manual technical procedures, mostly developed decades ago, which justifies the development of more sensitive and practical techniques. Therefore, the main objective of this study was to develop, evaluate, and validate a new parasitological technique referred to as TF-Test Modified, in comparison to three conventional parasitological techniques: TF-Test Conventional; Rugai, Mattos & Brisola; and Helm Test/Kato-Katz. For this realization, we collected stool samples from 457 volunteers located in endemic areas of Campinas, São Paulo, Brazil, and statistically compared the techniques. Intestinal protozoa and helminths were detected qualitatively in 42.23% (193/457) of the volunteers by TF-Test Modified technique, against 36.76% (168/457) by TF-Test Conventional, 5.03% (23/457) by Helm Test/Kato-Katz, and 4.16% (19/457) by Rugai, Mattos & Brisola. Furthermore, the new technique presented almost perfect kappa agreement in all evaluated parameters with 95% (P < 0.05) of estimation. The current study showed that the TF-Test Modified technique can be comprehensively used in the diagnosis of intestinal protozoa and helminths, and its greater diagnostic sensitivity should help improving the quality of laboratory diagnosis, population surveys, and control of intestinal parasites.

Production of autoantibodies associated with polyclonal activation in Yersinia enterocolitica 0:8-infected mice

Polyclonal lymphocyte stimulation is one of the immunomodulatory mechanisms induced by arthritogenic pathogens. In this study we examined the polyclonal activation potential of a virulent strain of Y. enterocolitica serotype O:8 (WA 2707+) and its plasmidless isogenic pair (WA 2707-). SPF Swiss mice were infected intragastrically and spleen cells were obtained on days 7, 14, 21, 28, 35 and 42 after infection. The number of cells secreting nonspecific immunoglobulins of IgG, IgM and IgA isotypes was determined by the ELISPOT technique. The presence of serum-specific antibodies was investigated by ELISA and the presence of autoantibodies by dot-blot assay. Although the patterns of infection of the two bacterial strains were almost the same, only the animals infected with the virulent strain presented clinical anomalies. Neither arthritic nor inflammatory signs were observed in the joints of the infected animals. The greatest activation observed was that of the nonspecific IgM-secreting cells, and their peak of secretion occurred between the 28th and the 42nd day after infection, for both strains of Y. enterocolitica O:8. Only the animals infected with the virulent strain (WA 2707+) produced IgG-specific antibodies in the serum, from the 28th day after infection. The serum of animals infected with either strain showed reactivity to all the autologous constituents tested, mainly on the 28th and 42nd day after infection. It was concluded that infection of mice with either the virulent strain of Y. enterocolitica O:8 or with its plasmidless isogenic pair resulted in the polyclonal activation of the splenic B lymphocytes including some autoreactive clones.

TF-Test modified: new diagnostic tool for human enteroparasitosis

Intestinal parasitosis is highly prevalent worldwide, being among the main causes of illness and death in humans. Currently, laboratory diagnosis of the intestinal parasites is accomplished through manual technical procedures, mostly developed decades ago, which justifies the development of more sensitive and practical techniques. Therefore, the main objective of this study was to develop, evaluate, and validate a new parasitological technique referred to as TF-Test Modified, in comparison to three conventional parasitological techniques: TF-Test Conventional; Rugai, Mattos & Brisola; and Helm Test/Kato-Katz. For this realization, we collected stool samples from 457 volunteers located in endemic areas of Campinas, São Paulo, Brazil, and statistically compared the techniques. Intestinal protozoa and helminths were detected qualitatively in 42.23% (193/457) of the volunteers by TF-Test Modified technique, against 36.76% (168/457) by TF-Test Conventional, 5.03% (23/457) by Helm Test/Kato-Katz, and 4.16% (19/457) by Rugai, Mattos & Brisola. Furthermore, the new technique presented “almost perfect kappa” agreement in all evaluated parameters with 95% (P < 0.05) of estimation. The current study showed that the TF-Test Modified technique can be comprehensively used in the diagnosis of intestinal protozoa and helminths, and its greater diagnostic sensitivity should help improving the quality of laboratory diagnosis, population surveys, and control of intestinal parasites.

Consultório odontológico: uma AET utilizando-se da EWA

This work tells the first steps of a larger project, that has for objective to develop the ergonomic evaluation of clinics odontologics installed in the Basic Units of Health of the municipal districts from São Paulo of São José dos Campos and Bauru. It is looked for to the end of the work to have characterized them, making possible like this the analysis in the way as it happens the interface among the users, equipments and atmosphere. For so much some will be used methodologies consecrated in the international scenery. It is told the results obtained with the first developed analysis, as test equal calibration of the chosen methodologies, as well as determination of procedures and worn-out times, for planning of the process.

Swarm-based descriptor combination and its application for image classification

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Tempos de barbante: declínio e revitalização da literatura de cordel. Da Primeira República à contemporaneidade

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Construção de um indicador qualitativo (status de saúde) para avaliação da qualidade de saúde animal de propriedades rurais

Aiming at developing a tool for assessing the magnitude of farm animal health, an indicator named herd “animal health status” was built. To illustrate the construction of the indicator, serum samples were taken from 923 goats on 17 goat farms in the state of Sao Paulo, Brazil, where three diseases were evaluated along with their impact percentages: caprine arthritis encephalitis, toxoplasmosis and neosporose – all of an infectious and transmissible nature and the cause of considerable economic losses. The mathematical rationale underlying the building of the “animal health status” indicator ranks properties on a 0 - 100% scale in terms of disease positivity in the herd, with the lowest indicator value indicating the highest cumulative disease frequency (0% = all animals tested positive for the three diseases, 100% = all animals tested negative for all diseases). Anti-T. gondii, anti-N. caninum and anti-CAEV antibodies were tested using indirect immunofluorescence reaction (IIFR ≥ 16), Neospora agglutination test (NAT ≥ 25) and agarose gel immunodiffusion (AGID positive or negative), respectively. The animal health status of the farms ranged from 32.38% to 96.40% according to disease positivity. The animal health status value will have a direct reflection on the production chain both on and off the farm, providing not only great market advantages, due to how the expression of animal health will add value to livestock farms, as well as on the consumer who can check the quality of animal health at the beginning of the production chain.

Comportamento de forrageamento de Surucuá-variado (Trogon Surrucura) (Aves, Trogonidae)

Os frugívoros desempenham papel fundamental na manutenção das florestas ao dispersarem as sementes dos frutos que consomem. Dentre as aves tropicais, a família Trogonidae é uma importante representante de tal relação ecológica e por serem sensíveis a alterações no ambiente, são bioindicadores da qualidade do habitat. O presente trabalho teve por objetivo descrever o comportamento de forrageamento de Trogon surrucura no Parque Estadual da Cantareira, São Paulo, e registrar e levantar na literatura os itens alimentares consumidos pela espécie. Foi observado que T. surrucura capturou presas preferencialmente em folhas verdes, utilizou-se da manobra investir-pairar na maior parte dos eventos de forrageamento, e não retornou aos poleiros de partida em 92% dos casos. Entre os itens alimentares levantados, os mais registrados foram artrópodes (principalmente insetos da família Tettigoniidae e lepidópteros), seguidos de frutos (Eugenia uniflora, Euterpe edulis entre outros), além de vertebrados (anuros da família Hylidae) e flores (do gênero Ipomoea). Como já citado em literatura, a espécie mostrou-se comum na área de estudo

Comportamento de forrageamento de Surucuá-variado (Trogon Surrucura) (Aves, Trogonidae)

Os frugívoros desempenham papel fundamental na manutenção das florestas ao dispersarem as sementes dos frutos que consomem. Dentre as aves tropicais, a família Trogonidae é uma importante representante de tal relação ecológica e por serem sensíveis a alterações no ambiente, são bioindicadores da qualidade do habitat. O presente trabalho teve por objetivo descrever o comportamento de forrageamento de Trogon surrucura no Parque Estadual da Cantareira, São Paulo, e registrar e levantar na literatura os itens alimentares consumidos pela espécie. Foi observado que T. surrucura capturou presas preferencialmente em folhas verdes, utilizou-se da manobra investir-pairar na maior parte dos eventos de forrageamento, e não retornou aos poleiros de partida em 92% dos casos. Entre os itens alimentares levantados, os mais registrados foram artrópodes (principalmente insetos da família Tettigoniidae e lepidópteros), seguidos de frutos (Eugenia uniflora, Euterpe edulis entre outros), além de vertebrados (anuros da família Hylidae) e flores (do gênero Ipomoea). Como já citado em literatura, a espécie mostrou-se comum na área de estudo

Inclusive J/psi production in pp collisions at root s=2.76 TeV

The ALICE Collaboration has measured inclusive J/psi production in pp collisions at a center-of-mass energy root s = 2.76 TeV at the LHC. The results presented in this Letter refer to the rapidity ranges vertical bar y vertical bar < 0.9 and 2.5 < y <4 and have been obtained by measuring the electron and muon pair decay channels, respectively. The integrated luminosities for the two channels are L-int(e) = 1.1 nb(-1) and L-int(mu) = 19.9 nb(-1), and the corresponding signal statistics are N-J/psi(e+e-) = 59 +/- 14 and N-J/psi(mu+mu-) = 1364 +/- 53. We present d sigma(J/psi)/dy for the two rapidity regions under study and, for the forward-y range, d(2)sigma(J/psi)/dydp(t) in the transverse momentum domain 0 < p(t) < 8 GeV/c. The results are compared with previously published results at root s = 7 TeV and with theoretical calculations. (C) 2012 CERN. Published by Elsevier B.V. All rights reserved.

J/psi Polarization in pp Collisions at root s=7 TeV

The ALICE Collaboration has studied J/psi production in pp collisions at root s = 7 TeV at the LHC through its muon pair decay. The polar and azimuthal angle distributions of the decay muons were measured, and results on the J/psi polarization parameters lambda(theta) and lambda(phi) were obtained. The study was performed in the kinematic region 2: 5 < y < 4, 2 < p(t) < 8 GeV/c, in the helicity and Collins-Soper reference frames. In both frames, the polarization parameters are compatible with zero, within uncertainties.

Homens, saúde e políticas públicas: a equidade de gênero em questão

O artigo tem por objetivo estabelecer questões acerca do tema Homens, Saúde e Políticas Públicas para a viabilização do debate sobre o assunto, com base em referências teóricas e empíricas relacionadas a essas questões. Inicialmente, alguns marcos históricos de temática são apresentados para que melhor se situe o debate. Em seguida, apresenta-se panorama da agenda de gênero nas políticas públicas para se introduzir a discussão acerca da inserção dessa perspectiva no âmbito das políticas de saúde. Após essa discussão, aborda-se o questionamento sobre o fato de as políticas de saúde dos homens promoverem ou não a equidade de gênero. Nas considerações finais, aponta-se para a complexidade que envolve a elaboração, a implementação e a avaliação das políticas de saúde que visam à equidade de gênero, bem como se destaca a necessidade de a política brasileira voltada para a saúde dos homens articular-se com outras políticas para que a matriz de gênero seja transversal no campo da saúde.