Expression and processing of Plasmodium berghei SERA3 during liver stages.

Cysteine proteases mediate liberation of Plasmodium berghei merozoites from infected hepatocytes. In an attempt to identify the responsible parasite proteases, we screened the genome of P. berghei for cysteine protease-encoding genes. RT-PCR analyses revealed that transcription of four out of five P. berghei serine repeat antigen (PbSERA) genes was strongly upregulated in late liver stages briefly before the parasitophorous vacuole membrane ruptured to release merozoites into the host cell cytoplasm, suggesting a role of PbSERA proteases in these processes. In order to characterize PbSERA3 processing, we raised an antiserum against a non-conserved region of the protein and generated a transgenic P. berghei strain expressing a TAP-tagged PbSERA3 under the control of the endogenous promoter. Immunofluorescence assays revealed that PbSERA3 leaks into the host cell cytoplasm during merozoite development, where it might contribute to host cell death or activate host cell proteases that execute cell death. Importantly, processed PbSERA3 has been detected by Western blot analysis in cell extracts of schizont-infected cells and merozoite-infected detached hepatic cells.

Ceramic formulation and processing design for plutonium disposition

The focus of this research programme is to develop a single phase ceramic wasteform for waste PuO2 that is unsuitable for fuel manufacture. A suite of synthetic mineral systems have been considered including titanate, zirconate, phosphate and silicate based matrices. Although a wealth of information on plutonium disposition in some of the systems exists in the literature, the data is not always directly comparable which hinders comparison between different ceramic hosts. The crux of this research has been to compile a database of information on the proposed hosts to allow impartial comparison of the relative merits of each system. © 2009 Materials Research Society.

The Jewel cutting and polishing industry in Puerto Rico.

Cover title.

Uruguay's livestock and meat industry.

Mode of access: Internet.

Data pertinent to a review of minimum wage rates established under the Fair labor standards act for newly covered employment in the banking, insurance, and finance industry in Puerto Rico /

Alternate pages blank.

Data pertinent to the establishment of minimum wage rates under the Fair labor standards act for employment newly covered by the amendments of 1966 in the hotel and motel industry in Puerto Rico /

"WH67-712."

Data pertinent to the establishment of minimum wage rates under the Fair labor standards act for newly covered employment in the laundry and cleaning industry in Puerto Rico /

"WH67-301"

Technological and economic assessment of manganese nodule mining and processing /

Mode of access: Internet.

Supplementary data pertaining to employee earnings pertinent to the review of minimum wage rates under the Fair Labor Standards Act in the hotel and motel industry in Puerto Rico /

"October 1968."

In the matter of: public hearing before Special Industry Committee no. 41-C for the paper, paper products, printing, and publishing industry in Puerto Rico. 17, 18, and 19 September, 1958 ... San Juan, Puerto Rico. Reporters: John F. Hrozencik [and] Genevieve Biolsi.

Maynard E. Pirsig, chairman.

In the matter of: Public hearing before Special industry committee no. 32-C for the paper, paper products, printing and publishing industry in Puerto Rico. August 29-30, 1957. Reporters: James A. Brownlee and John F. Hrozencik.

Mode of access: Internet.

Source separation collection and processing equipment : final report.

"January 1981"--Cover.