Modulação nitrérgica na regulação ocitocinérgica da secreção do peptídeo natriurético atrial em cardiomiócitos

Historicamente conhecida por suas ações sobre o sistema reprodutor, hoje se sabe que a ocitocina (OT) também pode contribuir para a regulação da homeostase cardiovascular e hidroeletrolítica. A OT é produzida nos núcleos supra-óptico e paraventricular do hipotálamo e liberada para o plasma a partir de terminais neurais da pituitária posterior, no entanto, muitos estudos identificaram locais extra-cerebrais de produção OT, incluindo o coração e o endotélio vascular. A ativação de seus receptores em células endoteliais, bem como em sistemas hipotalâmicos/hipofisários e cardíaco, pode resultar na produção de óxido nítrico (NO). O presente trabalho teve como objetivo verificar o papel do NO na regulação da secreção de peptídeo natriurético atrial (ANP) estimulada por OT em cultura primária de cardiomiócitos de embriões de camundongos. Para tal, corações de embriões de camundongos Balb C, com 19 a 21 dias de vida intra-uterina, foram isolados e cultivados para os ensaios com OT e demais substâncias interferentes na síntese de NO e GMPc seu segundo mensageiro. A adição de concentrações crescentes de OT (0.1, 1, 10 e 100 μM) induziu aumento proporcional na secreção de ANP e nitrato para o meio, confirmando a ação estimuladora da OT em cardiomiócitos. O bloqueio da liberação de ANP estimulada por OT (10 μM) foi observada após adição de Ornitina Vasotocina (CVI-OVT) (100 μM), um antagonista específico de OT. Este antagonista inibiu a secreção basal de ANP, quando adicionado individualmente, sugerindo que a OT pode atuar via mecanismo autócrino, tônico estimulatório sobre a secreção de ANP. Amplificação da secreção de ANP estimulada por OT (10 μM) foi observada após sua associação com L-NAME, um inibidor da sintase de óxido nítrico (NOS) (600 μM), e ODQ (100 μM), um inibidor da guanilato ciclase solúvel, sugerindo a ocorrência de feedback negativo nitrérgico na liberação de ANP estimulada por OT no cardiomiócito. Os resultados obtidos mostraram modulação nitrérgica inibidora sobre a secreção de ANP estimulada por OT.

Uma análise do espiritismo em Fortaleza-CE, com ênfase na expansão territorial do grupo espírita Paulo e Estevão (GEPE), na perspectiva de visibilidade do espaço religioso

Pós-graduação em Geografia - IGCE

O corpo da alma: cosmos, casa e corpo espírita kardecista

O objeto deste estudo é compreender e destacar o papel do corpo no campo religioso investindo em uma análise da corporeidade espírita kardecista brasileira a partir de suas representações sociais e imaginário. O esforço analítico e a distribuição dos capítulos estão baseados no esquema macro/microcósmico de “Cosmos-casa-corpo”. O primeiro capítulo situa a construção do Espiritismo Kardecista por seu codificador, o intelectual francês Hippolyte Rivail, conhecido por seu codinome, Allan Kardec (Paris, 1804-1869) e suas pretensões de unificar “ciência, filosofia e religião”, produzindo um Cosmos. O segundo capítulo apresenta o centro espírita, espaço sagrado de seu universo ritual. O terceiro capítulo está centrado no referencial semântico “corpo”, que surge como instrumento heurístico e recorte de análise. Analisando as concepções e imaginário sobre o corpo no Espiritismo Kardecista, o trabalho propõe que as relações entre o mundo espiritual, o centro espírita e corpo são determinantes para a compreensão da pessoa espírita.

Estado do Tocantins: política e religião a construção do espaço de representação tocantinense

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Morfo-anatomia e desenvolvimento pós-seminal de cinco espécies de Bromeliaceae

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Violência doméstica contra mulheres: Centro de Referência da mulher - Araraquara

Pós-graduação em Ciências Sociais - FCLAR

Diversidade sexual e ensino de ciências: buscando sentidos

Pós-graduação em Educação para a Ciência - FC

Superação da dormência em sementes de Brachiaria humidicola cv. BRS Tupi durante o armazenamento

Pós-graduação em Agronomia (Agricultura) - FCA

Arte naïf: da Santa Ceia aos Orixás

Pós-graduação em Artes - IA

Germination of Roystonea borinquena OF Cook (Arecaceae) Seeds

This work aimed to study the effect of the temperature on the seed germination and to describe the morphology and germination process of disseminules (seeds with stucked endocarp) of R. borinquena species. The fruits were harvested and had their pulp (exocarp and mesocarp) removed and the disseminules were shade dried. The experimental design was entirely randomized and six treatments were performed (temperature conditions: constant at 25, 30 and 35 degrees C; alternated at 25-35 and 20-30 degrees C; and room temperature) with four replications with 25 disseminules per plot, placed in plastic boxes (gerbox) containing vermiculite. The germination rate and germination speed index were calculated, and the data were submitted to the variance analysis, and the means compared by the Tukey test. For the morphological studies, the external and internal sides of the disseminules, the embryo and the representative germination stages were sketched. The samples were fixed in FAA (formalin - acetic acid - ethyl alcohol). It was concluded that the highest germination rate and faster germination were at the constant temperature of 35 degrees C and alternated of 25-35 degrees C. The seeds are albuminous, with a hard endosperm that takes almost the entire interior of the disseminules; the embryo is lateral, peripheral and poorly differentiated; the germination is adjacent.

Estado da arte sobre sexualidade e educação sexual: estudo analítico-descritivo de teses e dissertações produzidas na Universidade Estadual Paulista

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A magia em um texto técnico agronômico da antiguidade romana: o 'Opus agriculturae', de Paládio

This work analyses the chapter 35, book I, of the agricultural treatise Opus agriculturæ, written by Rutilius Taurus Aemilianus Palladius (V C.E.). In that chapter the author presents some recipes, called remedia, to protect the farm and the garden against weeds and weather phenomena, as blight and fogs. The magical practices are identified according to both the fundamental principles of magic (similarity, contiguity, contrariety), which rule magical thought, and some elements of magical symbology. As the author seems not to distinguish magic and science, for he brings together both kinds of recipes, the analysis of some remedia emphasizes a specific study on the materials and substances employed in those recipes and their value in Science today. This leads to the discussions of how magical thought works, what are the limits (if they actually exist) between Magic and Science and between Magic and Religion. This work covers the subjects above and it has the following pourposes: demonstrate the characteristics that authorize the remedia described by Palladius to be classified as folk magic; identify the relations between this kind of practice with more complex forms of magic, as with religion, with science; demonstrate the contribution of the folk magic in ancient Rome farming for the formulation of more apropriated criteria of evaluating magic in comparison to religious and scientific thought.

State of the art on animal embryonic chimeras

Embryonic chimerism is generally used in basic research and in vivo diagnosis of undifferentiated embryonic stem cells (ESC), mostly using mice embryos, although there have been reports in the literature on using rat, rabbit, sheep, chicken, primate, bovine, goat and pig embryos. Several techniques can currently be used to produce chimeric embryos, including microinjection, co-culture with ESC, fusion and aggregation. Although microinjection is the most commonly used method in mice, the mere aggregation of embryos with ESC may result in viable chimeras and be as efficient as microinjection. In mice, this chimerism technique has been shown to have the advantage of aggregating embryos in different stages of development with different ploidy, in addition to using ESC in the tetraploid complementation assay. Compared to other techniques for producing chimeras, the aggregation technique is a cheaper, faster and easier methodology to be performed. Moreover, aggregation can be simplified by chemically removing the zona pellucida with pronase or acidic Tyrode’s solution and be enhanced by using the Well of the Well culture system in combination with adhesion molecules, such as phytohemagglutinin. The most commonly used stages for chimerism by aggregation are those that precede the full compaction of the morula. In these stages, embryos have low-tension adherent junctions at the tangential point between two blastomeres. During the embryonic development of mice, the inner cell mass differentiates into epiblast and hypoblast. These layers will originate the fetal tissues and a portion of the extraembryonic tissues (yolk sac, allantois and amnion), whereas the trophectoderm (TE) gives rise to the chorion. A functional TE is essential for the complex molecular communications that occur between the embryo and the uterus. Embryos produced by somatic cell nuclear transfer, such as commercial cattle clones or endangered species, are subject to large fetal and neonatal losses. Hence embryo complementation with heterologous TE could be of assistance to decrease these losses and might as well assist development of high-value embryos in other approaches.

Valores priorizados por estudantes universitários de um curso de psicologia de uma universidade pública

Most research on the psychology of morality defines moral as the rules and values whose purpose is to regulate interpersonal relations. In this study, although I have considered such premise valid, I have also taken into account aspects related to the self. Thus, aiming at verifying whether university students are guided by values which are public, private and/or linked to glory, I have analyzed the values prioritized by them. Informants were 170 first-year psychology undergraduates in a public university located in western São Paulo state, male and female, with an average age of 20 years. To collect the data, I applied a questionnaire concerning factual factors (age, sex and religion) and the values cherished by the undergraduates. The results – analyzed according to the psychology of virtues – have shown that approximately 70% of the valid answers given by students pointed friendship (32%) and intelligence (35%) as the most prioritized values. Public values were hardly mentioned. Concerning the forms of glory, although the percentage for money and fame was not significant, when the informants justified the choice of friendship and intelligence, they conceived such values as a means to obtain money and fame. I have concluded that these individuals give little importance to the values related to duties (which are public) and to some form of individual harmony.

Sistema atende mulher: sistema de informação no centro de referência da mulher Ednalva Bezerra

The physical, psychological, sexual, moral and patrimonial violence affects women of different ages, social classes, ethnic and racial groups, levels of education and religion, all over Brazil. One of the obstacles in the strategies of prevention and facing violence against women, in public care services, focuses on the absence of an information system, automation of data and care flows. The existence of reliable data is essential for performing diagnoses, goals and conducting research on women in situations of violence who access the service agencies. In this sense, the goal of research is to present the experience of implementing a policy of data management called Woman System, in Ednalva Bezerra Women's Reference Center, which offers host/psychological and social care, guidance and legal referral for women that face any violence situation. As methodological paths, we adopt research in action. In our analyzes, we found that in this kind of public service, the use of the data system may improve the full and humanized care to women in situations of violence. Thus, the System Woman Care, still a developing phase, appears to improve the flow of relevant information within the institution, driving a process of knowledge, decision-making and intervention against gender violence.