Selective Formation of Sed5p-containing SNARE Complexes Is Mediated by Combinatorial Binding Interactions

Sed5p is the only syntaxin family member required for protein transport through the yeast Golgi and it is known to bind up to nine other soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) proteins in vivo. We describe in vitro binding experiments in which we identify ternary and quaternary Sed5p-containing SNARE complexes. The formation of SNARE complexes among these endoplasmic reticulum- and Golgi-localized proteins requires Sed5p and is syntaxin-selective. In addition, Sed5p-containing SNARE complexes form selectively and this selectivity is mediated by Sed5p-containing intermediates that discriminate among subsequent binding partners. Although many of these SNAREs have overlapping distributions in vivo, the SNAREs that form complexes with Sed5p in vitro reflect their functionally distinct locales. Although SNARE–SNARE interactions are promiscuous and a single SNARE protein is often found in more than one complex, both the biochemical as well as genetic analyses reported here suggest that this is not a result of nonselective direct substitution of one SNARE for another. Rather our data are consistent with the existence of multiple (perhaps parallel) trafficking pathways where Sed5p-containing SNARE complexes play overlapping and/or distinct functional roles.

Mathematical notebook, and commonplace book, circa 1689-1769

Manuscript notebook, possibly kept by Harvard students, containing 17th century English transcriptions of arithmetic and geometry texts, one of which is dated 1689-1690; 18th century transcriptions from John Ward’s “The Young Mathematician’s Guide”; and notes on physics lectures delivered by John Winthrop, the Hollis Professor of Mathematics and Natural Philosophy at Harvard from 1738 to 1779. The notebook also contains 18th century reading notes on Henry VIII, Tudor succession, and English history from Daniel Neal’s “The History of the Puritans” and David Hume’s “History of England,” and notes on Ancient history, taken mainly from Charles Rollin’s “The Ancient History of the Egyptians, Carthaginians, Assyrians, Babylonians, Medes and Persians, Macedonians and Grecians.” Additionally included are an excerpt from Plutarch’s “Lives” and transcriptions of three articles from “The Gentleman’s Magazine, and Historical Chronicle,” published in 1769: “A Critique on the Works of Ovid”; a book review of “A New Voyage to the West-Indies”; and “Genuine Anecdotes of Celebrated Writers, &.” The flyleaf contains the inscription “Semper boni aliquid operis facito ut diabolus te semper inveniat occupatum,” a variation on a quote of Saint Jerome that translates approximately as “Always good to do some work so that the devil may always find you occupied.” In the seventeenth and eighteenth centuries, Harvard College undergraduates often copied academic texts and lecture notes into personal notebooks in place of printed textbooks. Winthrop used Ward’s textbook in his class, while the books of Hume, Neal, and Rollin were used in history courses taught at Harvard in the 18th century.

L'opposition sous les Césars /

Où se trouvaient les mécontents.--L'opposition des gens du monde.--L'exil d'Ovide.--Les délateurs.--Un roman de moeurs sous Néron.--Les écrivains de l'opposition.

Les XV. livres de la Metamorphose d'Ovide, poete treselegant : contenants l'Olympe des hystoires poetiqves, traduitz de latin en françois.

Page numbers 383-384 repeated.

Ovid. Metamorphoses,

Latin and English on opposite pages.

Ovid and his influence,

Bibliography: p. 180-184.

Ovid and his influence.

Bibliography: p. 180-184.

Die Metamorphosen /

Includes bibliographical references and index.

Reports of cases in the Supreme Court of Appeals of Virginia.

Some vols. also contain reports of cases in the General Court of Virginia.

Saggi filologici.

Bibliographical footnotes.

De Ovidii Heroidum codice gissensi.

Vita.

Ibis, storia di un poemetto greco.

At head of title: A. Rostagni.

Ovidii Halieutica ; Gratii et Nemesiani Cynegetica /

Mode of access: Internet.

Obras de Ovidio /

Title taken from Vol. 3.

Selections from Ovid's Metamorphoses.

Mode of access: Internet.