Efeito da alimentação do adulto na qualidade do endoparasitoide larval da broca comum da cana-de-açúcar

Aiming qualitative improvements in the mass rearing of Cotesia flavipes (Cameron, 1891) (Hymenoptera: Braconidae) in laboratory, tests were conducted with adults feed before parasitism on Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae) larvae, using 14 artificial diets based on sucrose, brewer yeast and honey. The design was completely randomized with 15 treatments and five replications. The biological parameters evaluated in F1 and F2 generations were: number of adults emerged; sex ratio; adult emergence percentage; weight of pupae mass; and male and female longevity. The highest average of emerged adults were 85 in diet with sucrose 5% for F1 generation, and 91 in diet with sucrose 5% + yeast brewer 2,5% for F2; the smaller average of adult emergency were 63.60 in diet with honey 5% + yeast brewer 2.5 % for F1 generation and 66.80 in diet with honey 5% for F2. Diets that produced more females were honey 5% + yeast brewer 2.5%, showing the sex ratio of 0.82 for F1 generation, and sucrose 10% + 2.5% yeast brewer 2.5% (0.75) for F2, while sucrose 10% + yeast brewer 2.5% (0.60 for F1) and honey 2.5% (0.59) provided fewer females for F2 generation. The highest percentage of adult emergence were 93.90% with the diet containing sucrose 2.5% + yeast brewer 2.5%) and 93.89 % with honey 5% + yeast brewer 2.5%, and the lowest 81.71% (honey 5% + yeast brewer 2.5%) for F1 and 78.96 % (honey 5%) to F1 and F2, respectively. The diet with Mel 2.5% produced greater weight of pupae mass. The adult longevity did not differ significantly for all diets in F1 and F2 generations. Diets containing 5% or 10% of sucrose or honey provide, in general, improve the qualitative development of C. flavipes in mass rearing.

Advertisement call, colour variation, natural history, and geographic distribution of Proceratophrys caramaschii (Anura: Odontophrynidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Propagação de rambutanzeiro (Nephelium lappaceum L.) por enxertia

There is great genetic diversity of rambutan plants, thus, to establish commercial orchards culture, it is recommended the use of plants vegetatively propagated. Therefore, this research aimed to determine the best method of grafting, graft protection and type of rootstock for its propagation. The experiment was repeated in two seasons (autumn/winter and spring/summer). The experimental design was completely randomized in both experiments, with four replicates, each experimental unit consisted of 10 plants analyzed in a 4x2x2 factorial with four types of grafting (whip graft-WG; cleft graft -CG; wedge graft-WG; and inverted wedge graft-IWG;), 2 types of graft protection (Biodegradable and plastic) and 2 types of rootstock (without or with leaves). The variables analyzed in this study were: percentage of grafting success, number and length of buds (cm). For grafting performed in autumn /winter, both by cleft grafting as whip graft with biodegradable strip and rootstock leafless provided the best results in propagation of rambutan tree. The best results were observed during the autumn / winter.

Desempenho e uso eficiente da terra de modalidades de consorciação com milho e forrageiras

This study was developed with the aim of evaluating the performance and land use efficiency of different arrangements of intercropping with maize and forage. The field experiment was conducted in Tangar da Serra/MT. The experimental design was a randomized block in factorial scheme 3x2 + 1, were as three arrangements of intercropping, two species of forage and an additional treatment with maize monoculture in four replication. In maize monoculture and intercropping were evaluated: plant height and ear insertion height, plant population, weight of 100 grains, productivity, number of grain rows and number of grain per row. In forages intercropping with maize were evaluated plant population and dry matter accumulation. The arrangements with Maize + Forage sown in the line and Maize + Forage sown in two rows between rows with the species Urochloa hybrid promoted best corn yields. The modality and species affect both the productivity of maize as forages species. Grain yield of maize is higher in monoculture. The higher dry matter accumulation of Urochloa hybrid occurs in the Maize + Forage sown in one row between rows, while, for the Urochloa brizantha the arrangements studied do not affect their yields. The arrangement with best land use efficiency is Maize + Forage sown in two rows between rows for both study forage species.

Resposta funcional da joaninha Cryptolaemus predando Cochonilha branca em diferentes temperaturas e substratos vegetais

This work was carried out to evaluate the functional response of Cryptolaemus montrouzieri Mulsant, 1850 (Coleoptera: Coccinellidae) fed with Planococcus citri Risso, 1813 (Hemiptera: Pseudococcidae) reared on a pumpkin hybrid (Cucurbita maxima x Cucurbita moscata) (Cucurbitaceae), seedlings of Rangpur lime (Citrus limonia) Rutaceae) and potato (Solanum tuberosum) (Solanaceae) at two temperatures. The predation rate of C. montrouzieri was measured using Petri dishes of 15 cm diameter with 1, 2, 4, 8, 16 and 24 adults of P. Citri. One third instar larva, one fourfh instar and one newly emerged adult (without differentiation of sex) of C. montrouzieri were added to each plate. The study was conducted in climatic chambers at temperatures of 25 and 30 degrees C and photophase of 12 hours. The predation rate was evaluated after 24 hours of prey exposition to the predator, by counting the number of preys trapped in the different treatments and control. The statistical design was completely randomized with four treatments x 6 subplots with 7 repetitions, the two temperatures. The values obtained were subjected to analysis of variance, to relate the number of scales preyed by larvae and adults of C. montrouzieri set up in different substrates. The amount of prey consumed by larvae and adults of the predator increased with increasing the prey density until it reaches a plateau, characterizing functional response type II. In general, the number of scales preyed by larvae and adults of C. montrouzieri was higher on potato and under temperature of 30 degrees C.

Crecimiento, acumulación de macronutrientes y producción de melón cantaloupo y amarillo

Fueron conducidos dos experimentos individuales en el municipio de Mossoró, Rio Grande do Norte, Brazil. Se empleó dos cultivares de melón, ‘Olimpic express’ (del tipo Cantaloupo) e ‘Iracema’ (del tipo Amarillo), para evaluar el crecimiento y la acumulación de macronutrientes, bajo el diseño de bloques al azar con siete tratamientos (épocas de muestreo) y tres repeticiones. Los muestreos de plantas fueron realizados a los 14, 21, 28, 35, 42, 49 y 56 días después del trasplante (DDT). El crecimiento fue lento hasta 28 DDT en ambos cultivares evaluados, intensificándose en el periodo siguiente, alcanzando a los 56 DDT, 246.4g planta-1 , en ‘Olimpic express’,y 266.9 g planta-1, en ‘Iracema’, siendo la materia seca (MS) de los frutos correspondientes a 60% y 64% de la MS total, respectivamente. Mayores acumulaciones de N, P y K fueron obtenidos en los frutos, mientras de Ca, Mg y S en las hojas. Al final del ciclo, en ‘Olimpic express’, cuya productividad fue de 32 t ha-1, fueron acumulados 173.4, 110.1, 101.1, 26.9, 15.6 y 13.5 kg ha-1 de K, Ca, N, Mg, S y P, respectivamente, y en ‘Iracema’, cuya productividad fue de 38 t ha-1, fueron acumulados 136.0, 93.9, 84.1, 22.6, 15.4 y 9.5 kg ha-1 de K, N, Ca, Mg, S y P, respectivamente. En relación al total acumulado, las exportaciones de N, P, K, Ca, Mg y S en los frutos fueron de 61, 73, 66, 9, 35 y 39% (‘Olimpic express’)y 58, 70, 55, 6, 33 y 41% (‘Iracema’). Con una menor producción de frutos y una mayor acumulación de nutrientes, ‘Olimpic express’ ha demostrado ser menos eficiente en el uso de nutrientes que ‘Iracema’.

Frequência fenotípica de asas e olhos ao longo de gerações em populações de laboratório de chrysoperla externa (Neuroptera: Chrysopidae)

In massal rearing of natural enemies with the goal of biological control, the procedures adopted for establishment and maintenance of the individual founders of the colonies may have undesirable effects on population genetic structure of laboratory. This situation influences the success of rearing and effectiveness in the field. The objective of this study was to evaluate, along of generations two laboratory populations (Jaboticabal and Piracicaba) of Chrysoperla externa (Hagen) (Neuroptera: Chrysopidae), founded with different numbers of adults (1, 5, 10, 15 and 20 couples), the frequency of morphological variants, size of wings and eye color, such as parameters for inferences about the homozygosity degrees. For eye color were assessed the frequency, while for the size of wings was measured the width and the length of the right mesothoracic wings. The eye color variants for C. externa populations may be monitored in the laboratory aiming at detecting inbreeding, whereas the measurements of length and width of wings should not be adopted for this purpose.

Biologia comparada de populações da lagarta–do–cartucho em folhas de milho e mandioca

The fall armyworm is a pest that feeds on various botanies species. The objective of this experiment was to study the biology of this pest in corn and cassava leaves. Caterpillars were collected in area under cultivation with cassava and maintained on artificial diet for two generations. Under controlled conditions in a climatic chamber (B.O.D) in the laboratory (25 degrees, 60 +/- 10% RH and photophase 14 hours) were evaluated daily 50 caterpillars in corn treatments and 50 in cassava, where duration and viability of the larval phase and pupal, weight of pupas were observed after 24 hours, deformation percentage of pupas and adults, longevity, fecundity and total life cycle. The viability of larvae fed on leaves of maize and cassava was 74% and 60%, respectively. The larval period of the insects was shorter in maize 16.89 days (seven instars) and cassava 20.08 days (six instars). The pupal phase lasted 11.42 days in cassava treatment and 10.87 in the maize. The pupal weight of females and males was higher in corn 204.91 mg and 198.97 mg, respectively. The biological cycle varied depending on the ingested food. Adult longevity lasted 9.88 days for insects fed on cassava leaves. Therefore, cassava affected the development of S. frugiperda.

Plantas utilizadas para tratamento da malária e males associados em comunidades indígenas no rio Uapés em São Gabriel da Cachoeira - AM

Pós-graduação em Agronomia (Horticultura) - FCA

Filogeografia de Nasutitermes corniger (Isoptera: Termitidae: Nasutitermitinae) na região Neotropical

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Anatomy of extrafloral nectaries in Fabaceae from dry-seasonal forest in Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo fitoquímico e biológico dos extratos das folhas de Mimosa caesalpiniifolia Bentham

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo fitoquímico e biológico dos extratos das folhas de Mimosa caesalpiniifolia Bentham

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Phylogeny and cryptic diversity in geckos (Phyllopezus; Phyllodactylidae; Gekkota) from South America's open biomes

The gecko genus Phyllopezus occurs across South America's open biomes: Cerrado, Seasonally Dry Tropical Forests (SDTF, including Caatinga), and Chaco. We generated a multi-gene dataset and estimated phylogenetic relationships among described Phyllopezus taxa and related species. We included exemplars from both described Phyllopezus pollicaris subspecies, P. p. pollicaris and P. p. przewalskii. Phylogenies from the concatenated data as well as species trees constructed from individual gene trees were largely congruent. All phylogeny reconstruction methods showed Bogertia lutzae as the sister species of Phyllopezus maranjonensis, rendering Phyllopezus paraphyletic. We synonymized the monotypic genus Bogertia with Phyllopezus to maintain a taxonomy that is isomorphic with phylogenetic history. We recovered multiple, deeply divergent, cryptic lineages within P. pollicaris. These cryptic lineages possessed mtDNA distances equivalent to distances among other gekkotan sister taxa. Described P. pollicaris subspecies are not reciprocally monophyletic and current subspecific taxonomy does not accurately reflect evolutionary relationships among cryptic lineages. We highlight the conservation significance of these results in light of the ongoing habitat loss in South America's open biomes. (C) 2011 Elsevier Inc. All rights reserved.

Brazilian research on extremophiles in the context of astrobiology

Extremophiles are organisms adapted to grow at extreme ranges of environmental variables, such as high or low temperatures, acid or alkaline medium, high salt concentration, high pressures and so forth. Most extremophiles are micro-organisms that belong to the Archaea and Bacteria domains, and are widely spread across the world, which include the polar regions, volcanoes, deserts, deep oceanic sediments, hydrothermal vents, hypersaline lakes, acid and alkaline water bodies, and other extreme environments considered hostile to human life. Despite the tropical climate, Brazil has a wide range of ecosystems which include some permanent or seasonally extreme environments. For example, the Cerrado is a biome with very low soil pH with high Al+3 concentration, the mangroves in the Brazilian coast are anaerobic and saline, Pantanal has thousands of alkaline-saline lakes, the Caatinga arid and hot soils and the deep sea sediments in the Brazilian ocean shelf. These environments harbour extremophilic organisms that, coupled with the high natural biodiversity in Brazil, could be explored for different purposes. However, only a few projects in Brazil intended to study the extremophiles. In the frame of astrobiology, for example, these organisms could provide important models for defining the limits of life and hypothesize about life outside Earth. Brazilian microbiologists have, however, studied the extremophilic micro-organisms inhabiting non-Brazilian environments, such as the Antarctic continent. The experience and previous results obtained from the Brazilian Antarctic Program (PROANTAR) provide important results that are directly related to astrobiology. This article is a brief synopsis of the Brazilian experience in researching extremophiles, indicating the most important results related to astrobiology and some future perspectives in this area. Received 29 February 2012, accepted 25 May 2012, first published online 11 July 2012