Proteinograma sérico, com ênfase em proteínas de fase aguda, de bovinos sadios e bovinos portadores de enfermidade aguda de ocorrência natural

Nas últimas décadas, as proteínas de fase aguda (PFAs) tornaram-se biomarcadores de escolha em medicina humana para identificação e monitoração de doenças. Não há razão para imaginar que tais pesquisas clínicas não sejam igualmente úteis na medicina veterinária. Com o objetivo de verificar a importância das PFAs como biomarcadores de doenças inflamatórias em bovinos, determinou-se o proteinograma sérico, por meio da técnica de eletroforese SDS-PAGE, com interesse especial nas PFAs. Foram utilizados 30 animais, distribuídos em dois grupos: 15 bovinos sadios e 15 bovinos doentes (cinco com mastite estafilocócica, cinco com fotossensibilização e cinco com onfaloflebite). Os animais foram submetidos a colheitas diárias de sangue durante sete dias, enquanto internados no Hospital Veterinário da Unesp, Campus de Jaboticabal. Ceruloplasmina e haptoglobina apresentaram elevação significativa em animais acometidos por mastite, fotossensibilização e onfaloflebite (275,17% e 343,71%; 175,17% e 230,19%; 114,47% e 144,47%, respectivamente). A α1-glicoproteína ácida foi um bom biomarcador apenas em animais com mastite e fotossensibilização, elevando, respectivamente, suas concentrações séricas em 198,14% e 145,89%. Fibrinogênio mostrou-se um indicador confiável apenas em bovinos com mastite, com elevação de 146,5% em relação ao grupo sadio. Ficou clara a diferença na responsividade de distintas PFAs frente a diferentes estímulos inflamatórios. Ceruloplasmina e haptoglobina foram biomarcadores mais sensíveis e, portanto, mais confiáveis entre as PFAs estudadas nessa espécie.

Qualidade microbiológica e vida útil de filés defumados de tilápia-do-nilo sob refrigeração ou congelamento

The objective of this work was to evaluate the microbiological quality and shelf life of Nile tilapia fillets subjected to different smoking methods and storing conditions. Two smoking processes (cold or hot) were used in fillets with or without pigmentation. Products were stored under refrigeration or freezing, and monitored continually for 28 days for evaluation of their shelf life. Frozen fillets were monitored for 146 days for analysis of thiobarbituric acid (TBA) only. Hot- and cold-smoking reduced the coliform quantity, respectively, by 99.78% and 97.80%. Product storage under refrigeration allowed a 99.73% coliform reduction, and storage under freezing reduced them by 99.83%. Fecal coliform values were within the allowed limits. TBA values in fillets reached their maximum on the 14th storage day. TBA values were higher in treatments under refrigeration storage than in those under freezing, as well as in cold-smoked fillets in comparison to the hot-smoked ones. Hot-smoked process, followed by refrigeration storage, is the most adequate technique to ensure quality and a larger shelf life for Nile tilapia fillets, regardless of pigmentation process.

Efeito dos óleos essenciais de Syzygium aromaticum e Cinnamomum zeylanicume e seus compostos majoritários na produçao de biofilme em cepas de Staphylococcus aureus, isolados de leite de vacas com mastite subclínica

Pós-graduação em Biologia Geral e Aplicada - IBB

Avaliação microbiológica de carcaças congeladas de perus adquiridas no comércio varejista

Pós-graduação em Medicina Veterinária - FCAV

Tratamento da mastite subclínica de ovelhas no período seco com antimicrobiano convencional e antimicrobiano nanoparticulado

Pós-graduação em Medicina Veterinária - FCAV

Avaliação da qualidade microbiológica de um composto produzido a partir de resíduos animais e vegetais

Pós-graduação em Microbiologia Agropecuária - FCAV

CARACTERIZAÇÃO DO STAPHYLOCOCCUS COAGULASE NEGATIVO ISOLADOS DE QUARTOS MAMÁRIOS DE VACAS COM MASTITE SUBCLÍNICA

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Indicadores de qualidade do leite bovino orgânico em duas propriedades leiteiras certificadas do Estado de São Paulo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Escolaridade e volume de produção têm associação com a percepção de risco de produtores de leite no uso de produtos veterinários

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A comparative study of production performance and animal health practices in organic and conventional dairy systems

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Microbiological quality of whole and filleted shelf-tilapia

Despite fish being a rich source of animal nutrients and having numerous associated health benefits, it is an extremely perishable food, prone to a wide range of hazards. The bacterial load associated with shelf-whole-fish organs (e.g. digestive tracts and skin) or mishandling of fish may be a vehicle of infection and become a risk to public health. The objective of this paper is to evaluate the microbiological quality of whole ungutted and filleted shelf-tilapia, as well as assess the safety for human consumption. For this purpose, in order to investigate the distribution and occurrence of bacterial populations, the count of total and thermotolerant coliforms, coagulase-positive Staphylococcus and presence of Salmonella spp. was determined. This paper shows that all fish organs were contaminated with thermotolerant coliform. Skin and fillet show higher populations and occurrence of all microorganisms analyzed. Lower bacterial populations were recovered from the gut and muscles of whole tilapia. Two samples of fillet were contaminated with coagulase-positive Staphylococcus. It can be concluded that the skin and filleted tilapia are important carriers of food-borne pathogens. In addition, fish might become an important cross and self-contamination source. (C) 2014 Elsevier B.V. All rights reserved.

Identification of Corynebacterium spp. isolated from bovine intramammary infections by matrix-assisted laser desorption ionization-time of flight mass spectrometry

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Uso de maltodextrina e prebiótico em rações de leitões desmamados

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Identificação de patógenos e qualidade higiênico-sanitária do caldo de cana (garapa), consumido no interior do estado de São Paulo

The state of São Paulo is the largest producer of cane sugar in Brazil, with approximately 346 million tons in 2008/2009. This great production, associated with hot weather favors trade in sugar cane juice in most cities in the state. Contamination by parasites usually occurs during production but the contamination by bacteria is related with foodhandlers, equipment (grinders) and utensils used in the extraction. Due the lack of data in Botucatu and region, the objective of this study was to analyze 50 samples of syrup, according to the microbiological requirements of RDC Nº 12 (determination of Most Probable Number of thermotolerant coliform and the presence of Salmonella sp) as well the presence of Staphylococcus aureus and Trypanosoma cruzi. Salmonella sp., Staphylococcus aureus and Trypanosoma cruzi were not found, however 82% of the samples presented a higher contamination by thermotolerant coliform than that allowed by microbiological parameters, demonstrating inadequate conditions for retail, besides the lack of hygienic instructions of the sellers

Atividade antimicrobiana da violaceína em linhagens isoladas de mastite bovina

Violacein is a deeply violet pigment produced by Chromobacterium violaceum, an ubique tropical and subtropical bacterium. This molecule presents many biological activities including antimicrobial, antimycotic, antiviral, antiprotozoal and antitumoral. Important reports pointed to a considerable bactericide activity, which brings an extremely necessary quest. This work evaluated the resistance of some bacterias of interest veterinary. It was obtained significant inhibition activity against Staphylococcus aureus came from bovine mastitis. It was also obtained important synergic relations when violacein and penicillin or cloranphenicol are combined