Indicadores das condições nutricionais na região Polonoroeste: V. Desnutrição protéico-energética e parasitoses intestinais em um grupo de crianças de 3 a 72 meses de idade da cidade de Mirassol D'Oeste, Mato Grosso, Brasil

Este trabalho tem por objetivo caracterizar a desnutrição protéico-energética associada a parasitose intestinal em grupo de 149 crianças de ambos os sexos, na faixa etária de 3 a 72 meses, da cidade de Mirassol D'Oeste, na região do Projeto Polonoroeste em Mato Grosso. De cada criança foram coletados os seguintes dados: sexo, peso, idade e amostra de fezes para exame parasitológico. Os dados peso/idade obtidos foram analisados pelos critérios de GOMEZ. Utilizou-se como padrão de referência o National Center for Health Statistic (NCHS). Para diagnóstico dos parasitas intestinais executou-se o método de Hoffman, Pons e Janer. O grupo estudado constitui-se em sua maioria de crianças desnutridas, sendo a forma leve de desnutrição mais comum que as formas moderada e grave. As enteroparasitoses foram encontradas em 69% das amostras examinadas. A "Giardia lamblia" foi o protozoário mais comum e o "Ancilostomídeo" o helminto mais encontrado. O teste X² não mostrou relação de dependência entre o estado nutricional e a freqüência de enteroparasitoses.

O recrutamento das elites políticas no Brasil: o caso de Mato Grosso, 1945-2007

Tese apresentada para cumprimento dos requisitos necessários à obtenção do grau de Doutor em Ciência Política

Distribution of phlebotomine sandflies (Diptera: Psychodidae) of medical importance in Mato Grosso State, Brazil

Intense environmental impacts, causing alterations of the natural habitats of fauna, including those of sandfly disease vectors are observed in Mato Grosso State, Central Brazil. Entomologic survey of phlebotomines was based on light trap and was carried out by entomological nucleus of the FUNASA and SES in the period between 1996 and 2001. Eighty eight species were identified, including the following sandflies with medical importance to leishmaniasis: Lutzomyia amazonensis, L. anduzei, L. antunesi, L. ayrozai, L. carrerai carrerai, L. complexa, L. cruzi, L. flaviscutellata, L. intermedia, L. longipalpis, L. migonei, L. paraensis, L. ubiquitalis, L. whitmani and L. yuilli yuilli. Most sandflies of medical importance occurred in the Amazon forest and savannah. L. longipalpis and L. cruzi had high densities in the savannah region. L. flaviscutellata is predominating in both the Amazon forest and the savannah region. L. whitmani and L. antunesi were sampled in the Amazon forest, savannah and marsh land.

Fungal agents in different anatomical sites in Public Health Services in Cuiabá, state of Mato Grosso, Brazil

INTRODUCTION: A contribution to the regional epidemiological profile of the most common fungal agents in Public Health Services in Cuiabá, state of Mato Grosso, including university hospitals and polyclinics. METHODS: Clinical specimens (n = 1,496) from 1,078 patients were collected, submitted to direct mycological exam (potash or stick tape method) and cultured in specific mediums. Dermatophytic and non-dermatophytic agents were identified according to micromorphology (Ridell technique). RESULTS: The majority of the 1,496 specimens were skin (n = 985) and nail exams (n = 472). Of the 800 positive cultures, 246 (30.8%) corresponded to dermatophytes and 336 (42%) to yeasts of the genus Candida, 190 (23.7%) to other yeasts, 27 (3.4%) to non-dermatophytic filamentous fungi and one (0.1%) the agent of subcutaneous mycosis. Lesions considered primary occurred in greater numbers (59.5%) than recurrent lesions (37.4%), with a greater concentration of positivity occurring on the arms and legs. CONCLUSIONS: Comorbidities, allergies and diabetes mellitus were conditions associated with greater positivity in direct mycological exams and cultures. Positive culture was considered a definitive diagnosis of fungal infection and confirmed 47.8% of diagnostic hypotheses.

FIRST REPORT ON Cryptococcus neoformans IN PIGEON EXCRETA FROM PUBLIC AND RESIDENTIAL LOCATIONS IN THE METROPOLITAN AREA OF CUIABÁ, STATE OF MATO GROSSO, BRAZIL

SUMMARYCryptococcosis is a severe systemic mycosis caused by two species of Cryptococcus that affect humans and animals: C. neoformans and C. gattii. Cosmopolitan and emergent, the mycosis results from the interaction between a susceptible host and the environment. The occurrence of C. neoformanswas evaluated in 122 samples of dried pigeon excreta collected in 49 locations in the City of Cuiabá, State of Mato Grosso, Brazil, including public squares (n = 5), churches (n = 4), educational institutions (n = 3), health units (n = 8), open areas covered with asbestos (n = 4), residences (n = 23), factory (n = 1) and a prison (n = 1). Samples collected from July to December of 2010 were seeded on Niger seed agar (NSA). Dark brown colonies were identified by urease test, carbon source assimilation tests and canavanine-glycine-bromothymol blue medium. Polymerase chain reaction primer pairs specific for C. neoformans were also used for identification. Cryptococcus neoformans associated to pigeon excreta was isolated from eight (6.6%) samples corresponding to six (12.2%) locations.Cryptococcus neoformans was isolated from urban areas, predominantly in residences, constituting a risk of acquiring the disease by immunocompromised and immunocompetent individuals.

SEROLOGICAL MARKERS OF VIRAL, SYPHILITIC AND TOXOPLASMIC INFECTION IN CHILDREN AND TEENAGERS WITH NEPHROTIC SYNDROME: CASE SERIES FROM MATO GROSSO STATE, BRAZIL

Some infections can be the cause of secondary nephrotic syndrome. The aim of this study was to describe the experience of a Renal Disease Reference Clinic from Central Brazil, in which serological markers of some infectious agents are systematically screened in children with nephrotic syndrome. Data were obtained from the assessment of medical files of all children under fifteen years of age, who matched nephrotic syndrome criteria. Subjects were tested for IgG and IgM antibodies against T. gondii and cytomegalovirus; antibodies against Herpes simplex, hepatitis C virus and HIV; and surface antigen (HBsAg) of hepatitis B virus. The VDRL test was also performed. 169 cases were studied. The median age on the first visit was 44 months and 103 (60.9%) patients were male. Anti-CMV IgG and IgM were found in 70.4% and 4.1%, respectively. IgG and IgM against Toxoplasma gondii were present in 32.5% and 5.3%, respectively. Two patients were positive for HBsAg, but none showed markers for HIV, hepatitis C, or Treponema pallidum. IgG and IgM against herpes simplex virus were performed on 54 patients, of which 48.1% and 22.2% were positive. IgM antibodies in some children with clinical signs of recent infection suggest that these diseases may play a role in the genesis of nephrotic syndrome.

SAINT LOUIS ENCEPHALITIS VIRUS IN MATO GROSSO, CENTRAL-WESTERN BRAZIL

The dengue virus (DENV), which is frequently involved in large epidemics, and the yellow fever virus (YFV), which is responsible for sporadic sylvatic outbreaks, are considered the most important flaviviruses circulating in Brazil. Because of that, laboratorial diagnosis of acute undifferentiated febrile illness during epidemic periods is frequently directed towards these viruses, which may eventually hinder the detection of other circulating flaviviruses, including the Saint Louis encephalitis virus (SLEV), which is widely dispersed across the Americas. The aim of this study was to conduct a molecular investigation of 11 flaviviruses using 604 serum samples obtained from patients during a large dengue fever outbreak in the state of Mato Grosso (MT) between 2011 and 2012. Simultaneously, 3,433 female Culex spp. collected with Nasci aspirators in the city of Cuiabá, MT, in 2013, and allocated to 409 pools containing 1-10 mosquitoes, were also tested by multiplex semi-nested reverse transcription PCR for the same flaviviruses. SLEV was detected in three patients co-infected with DENV-4 from the cities of Cuiabá and Várzea Grande. One of them was a triple co-infection with DENV-1. None of them mentioned recent travel or access to sylvatic/rural regions, indicating that transmission might have occurred within the metropolitan area. Regarding mosquito samples, one pool containing one Culex quinquefasciatus female was positive for SLEV, with a minimum infection rate (MIR) of 0.29 per 1000 specimens of this species. Phylogenetic analysis indicates both human and mosquito SLEV cluster, with isolates from genotype V-A obtained from animals in the Amazon region, in the state of Pará. This is the first report of SLEV molecular identification in MT.

DENGUE OUTBREAK IN MATO GROSSO STATE, MIDWESTERN BRAZIL

Dengue virus (DENV) is the most frequent arbovirus worldwide. In this study, we report a large outbreak in Mato Grosso State (MT). Serum samples from 604 patients with acute febrile illness for less than five days were inoculated in C6/36 cells, then infected cells were subjected to an indirect immunofluorescence test for DENV serotypes and yellow fever virus. Serum samples were submitted to a multiplex-semi-nested-RT-PCR for 11 flaviviruses. DENV-4 was isolated in 150/604 (24.8%) and DENV-1 in 19/604 (3.1%) specimens. By RT-PCR, 331 (54.8%) samples tested positive for DENV; 321 had single infections (DENV-4 n = 305; DENV-1 n = 15; DENV-3 n = 1), nine had co-infections of DENV-1/DENV-4, and one of DENV-2/DENV-4. DENV-4 was detected in 315/331 (95.2%) positive patients from 17 municipalities, and DENV-1 in 24/331 (7.2%) patients from five cities in north-central MT and the city of Cuiaba. The incidence of infection was higher in patients aged 20-39 (142/331; 42.9%). The NS5 partial nucleotide sequence of DENV-1 was most similar to that of genotype V, DENV-2 to Southeast Asian/American, DENV-3 to genotype III, and DENV-4 to genotype II strains, considered the most frequent strains in Brazil. This outbreak coincided with the introduction of DENV-4 in the state. Cuiaba was hyperendemic for the four DENV serotypes, highlighting the necessity for arbovirus surveillance in MT.

Contribuição ao estudo da esquistossomose e das enteroparasitoses em Mato Grosso: relato dos primeiros três casos autoctones de esquistossomose mansoni no Estado de Mato Grosso

Num total de 19.463 exames de fezes, realizados de 19 de janeiro de 1972 a 31 de dezembro de 1977 no Hospital Santa Maria Bertila de Guiratinga (Mato Grosso), 270 apresentaram ovos vivos de Schistosoma mansoni. A distribuição por sexo dos portadores de esquistossomose foi a seguinte: 167 homens (61,8%) e 103 mulheres (38,2%). Com referência à naturalidade, 229 pacientes eram naturais do Estado de Minas Gerais, 17 de Pernambuco, 14 da Bahia, quatro de Alagoas, três do Piauí e três de Mato Grosso. Os três pacientes de Mato Grosso são do sexo feminino, com 10, 17 e 23 anos. Dois são naturais e residem no município do Rio Negro e um reside no município de Rondonópolis sendo natural de Poxoréo. Os três nunca sairam do Estado. Achamos que estes casos revestem-se de grande importância epidemiológica pois que representam a primeira constatação de esquistossomose mansoni autóctone no Estado do Mato Grosso. Ê provável que esteja acontencendo em Mato Grosso o que aconteceu recentemente em Goiás: a implantação em área virgem de focos autóctones de Schistosoma mansoni, através de portadores vindos de zonas endêmicas de outros estados, particularmente Minas Gerais, Bahia e estados do Nordeste.

Hepatite Delta no Estado de Mato Grosso: apresentação de cinco casos

Cinco casos graves de infecção pelos vírus das hepatites B e D foram diagnosticados em jovens oriundos do norte do Mato Grosso, onde é comum a ocorrência de hepatite B, mas não de hepatite D. A proximidade com os Estados do Acre e do Amazonas e a migração interna podem explicar a introdução do vírus da hepatite D na região. Os autores salientam a necessidade de manter vigilância epidemiológica para casos de hepatite D na região.

Avaliação de genótipos de girassol em Mato Grosso, na safrinha de 2014.

RESUMO: Objetivou-se avaliar o desempenho agronômico de genótipos de girassol, em ensaio da Rede de Ensaios de Avaliação de Genótipos de Girassol, na safrinha de 2014, visando à indicação para cultivo no Estado de Mato Grosso. O experimento foi conduzido na área experimental do Instituto Federal de Mato Grosso (IFMT), campus de São Vicente - MT, na safrinha de 2014, empregando-se delineamento em blocos ao acaso, com quatro repetições. Foram efetuadas avaliações de altura de planta, rendimento de aquênios, teor de óleo e rendimento de óleo. Os resultados foram submetidos à análise de variância e as médias foram comparadas pelo teste de Duncan ao nível de 5% de significância. Os genótipos GNZ NEON, SYN 045, AGUARÁ 06, M734, MG 360 e MG 305 foram superiores na avaliação de rendimento de aquênios. Em teor de óleo, os genótipos MG 360, MG 305, HLA 2012, SYN 3950HO, PARAÍSO 20 e AGUARÁ 04 se destacaram. O intenso ataque de pássaros prejudicou o desempenho dos genótipos de girassol. ABSTRACT: This work aimed to evaluate the agronomic performance of the sunflower genotypes under testing by the Network of Evaluative Experiments with Sunflower Genotypes, on off-season of 2014, proposing an indication for cultivation in the state of Mato Grosso, Brazil. Experiment was conducted in the Federal Institute of Mato Grosso, campus of São Vicente - MT, using randomized complete block design, with four replications. Measurements were made, evaluating plant height, achene yield, oil content and oil yield. The results were submitted to analysis of variance and compared using the Duncan test at 5% of significance level. Genotypes GNZ NEON, SYN 045, AGUARÁ 06, M734, MG 360 and MG 305 were higher in achenes yield evaluation. Regarding oil content, the genotypes MG 360, MG 305, HLA 2012, SYN 3950HO, PARAISO 20 and AGUARÁ 04 were best. The intense attack of birds affected performance of sunflower genotypes.

Associação entre a produção anual de ouro em garimpos e incidência de malária em Mato Grosso - Brasil, 1985-1996

Baseado em dados secundários realizou-se análise epidemiológica do tipo ecológico da série histórica da incidência parasitária anual da malária, produção oficial anual garimpeira de ouro e gastos financeiros do Programa de Controle da Malária na Bacia Amazônica relativo ao Estado de Mato Grosso, no período de 1985-1996. Associação positiva e estatisticamente significante (p<0,001) entre produção de ouro e IPA foi observada em análise multivariada, ainda que controlada por gastos financeiros. Esse achado contribui para a elucidação da tendência de redução da malária em MT, observada na última década.