Nuclear medicine instrumentation development : DOE program study group report.

"UC-48."

Program Trixy : multi-group analysis of nuclear reactors in three space dimensions : for the IBM type 704 computer /

"Physics and Math. TID-4500 (15th Ed.)"--Title page.

Oversight--the Nuclear Regulatory Commission research program [microform] : hearings before the Subcommittee on Energy Research and Production of the Committee on Science and Technology, U.S. House of Representatives, Ninety-seventh Congress, second session, May 18, 19; June 15, 1982.

"No. 128."

The Illinois Spent Nuclear Fuel and High-Level Waste Inspection and Escort Program /

"5/99"--Colophon.

Nuclear medicine technology program.

Mode of access: Internet.

100,000,000 miles safely steamed on nuclear power : the United States Naval Nuclear Propulsion Program, January 1955 - April 1994.

Shipping list no.: 94-0322-P.

Nuclear waste : quarterly report on DOE's nuclear waste program as of December 31, 1985 : report to Congressional requesters /

"B-202377"--P. [1].

Review of the magnetic fusion program of the Department of Energy : hearing before the Subcommittee on Fossil and Nuclear Energy Research, Development and Demonstration of the Committee on Science and Technology, U.S. House of Representatives, Ninety-fifth Congress, second session, September 18, 1978.

"No. 86."

MHD program review : hearings before the Subcommittee on Fossil and Nuclear Energy Research, Development and Demonstration of the Committee on Science and Technology, U.S. House of Representatives, Ninety-fifth Congress, second session, May 2, 4, August 3, 1978.

"No. 76."

Department of Energy : DOE's nuclear safety enforcement program should be strengthened : report to the Chairman, Committee on Commerce, House of Representatives /

"B-282435"--P. 1.

Department of Energy's civilian Nuclear Waste Program : hearing before the Committee on Energy and Natural Resources, United States Senate, One Hundred Second Congress ....

Shipping list no.: 91-436-P (pt. [1]).

Nuclear waste program : hearings before the Committee on Energy and Natural Resources, United States Senate, One hundredth Congress, first session ...

"June 29, 1987"--Pt. 4.

Nuclear and mitochondrial DNA markers in traceability of retail beef samples

Several characteristics are important in a traceability system of animal products, such as age at slaughter, breed composition, besides information of the productive chain. In general, the certification agent records information about the animals and the system which it came from, although cannot guarantee that the slaughtering, meat processing and distribution are error proof. Besides, there is a differential price, at least at the international market, based on sex and breed composition of the animals. Genetic markers allow identification of characteristics controlled in the beef cattle traceability program, as sex and breed composition, in order to correctly identify and appraise the final product for the consumer. The hypothesis of this study was that the majority beef samples retailed in the local market originate from female with a great participation of zebu breeds. Therefore, the objective of this work was to characterize retail beef samples with DNA markers that identify cattle sex and breed composition. Within 10 beef shops localized in Pirassununga, SP, Brazil, 61 samples were collected, all were genotyped as harboring Bos taurus mitochondrial DNA and 18 were positive for the Y chromosome amplification (male). For the marker sat1711b-Msp I the frequency of the allele A was 0.278 and for the marker Lhr-Hha I the frequency of the allele T was 0.417. The results of sat1711b-Msp I and Lhr-Hha I allelic frequencies are suggestive that the proportion of indicus genome compared with the taurine genome in the market meat is smaller than the observed in the Nellore breed. The procedure described in this study identified sex and subspecies characteristics of beef meat samples, with potential application in meat products certification in special as an auxiliary tool in beef cattle traceability programs.

Haplotype distribution of five nuclear genes based on network genealogies and Bayesian inference indicates that Trypanosoma cruzi hybrid strains are polyphyletic

Chagas disease is still a major public health problem in Latin America. Its causative agent, Trypanosoma cruzi, can be typed into three major groups, T. cruzi I, T. cruzi II and hybrids. These groups each have specific genetic characteristics and epidemiological distributions. Several highly virulent strains are found in the hybrid group; their origin is still a matter of debate. The null hypothesis is that the hybrids are of polyphyletic origin, evolving independently from various hybridization events. The alternative hypothesis is that all extant hybrid strains originated from a single hybridization event. We sequenced both alleles of genes encoding EF-1 alpha, actin and SSU rDNA of 26 T. cruzi strains and DHFR-TS and TR of 12 strains. This information was used for network genealogy analysis and Bayesian phylogenies. We found T. cruzi I and T. cruzi II to be monophyletic and that all hybrids had different combinations of T. cruzi I and T. cruzi II haplotypes plus hybrid-specific haplotypes. Bootstrap values (networks) and posterior probabilities (Bayesian phylogenies) of clades supporting the monophyly of hybrids were far below the 95% confidence interval, indicating that the hybrid group is polyphyletic. We hypothesize that T. cruzi I and T. cruzi II are two different species and that the hybrids are extant representatives of independent events of genome hybridization, which sporadically have sufficient fitness to impact on the epidemiology of Chagas disease.

Autoinhibition by an internal nuclear localization signal revealed by the crystal structure of mammalian importin alpha

Importin alpha is the nuclear import receptor that recognizes classical monopartite and bipartite nuclear localization signals (NLSs). The structure of mouse importin alpha has been determined at 2.5 Angstrom resolution. The structure shows a large C-terminal domain containing armadillo repeats, and a less structured N-terminal importin beta-binding domain containing an internal NLS bound to the NLS-binding site. The structure explains the regulatory switch between the cytoplasmic, high-affinity form, and the nuclear, low-affinity form for NLS binding of the nuclear import receptor predicted by the current models of nuclear import. Importin beta conceivably converts the low- to high-affinity form by binding to a site overlapping the autoinhibitory sequence. The structure also has implications for understanding NLS recognition, and the structures of armadillo and HEAT repeats.