Development of a rapid, multi-class method for the confirmatory analysis of anti-inflammatory drugs in bovine milk using liquid chromatography tandem mass spectrometry

A rapid liquid chromatography tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the simultaneous identification, confirmation and quantitation of seven licensed anti-inflammatory drugs (AIDS) in bovine milk. The method was validated in accordance with the criteria defined in Commission Decision 2002/657/EC. Two classes of AIDS were investigated, corticosteroids and non-steroidal anti-inflammatory drugs (NSAIDs). The developed method is capable of detecting and confirming dexamethasone (DXM), betamethasone (BTM), prednisolone (FRED), tolfenamic acid (TV), 5-hydroxy flunixin (5-OH-FLU). meloxicam (MLX) and 4-methyl amino antipyrine (4-MAA) at their associated maximum residue limits (MRLs). These compounds represent all the corticosteroids and NSAIDs licensed for use in bovine animals producing milk for human consumption. These compounds have never been analysed before in the same method and also 4-methyl amino antipyrine has never been analysed with the other licensed NSAIDs. The method can be considered rapid as permits the analysis of up to 30 samples in one day. Milk samples are extracted with acetonitrile; sodium chloride is added to aid partition of the milk and acetonitrile mixture. The acetonitrile extract is then subjected to liquid-liquid purification by the addition of hexane. The purified extract is finally evaporated to dryness and reconstituted in a water/acetonitrile mixture and determination is carried out by LC-MS/MS. Decision limit (CC alpha) values and detection capability (CC beta) values have been established for each compound. (C) 2009 Elsevier B.V. All rights reserved.

Novel Radio Link Buffer Management Schemes for End-User Multi-class Traffic in High Speed Packet Access Networks

The requirement to provide multimedia services with QoS support in mobile networks has led to standardization and deployment of high speed data access technologies such as the High Speed Downlink Packet Access (HSDPA) system. HSDPA improves downlink packet data and multimedia services support in WCDMA-based cellular networks. As is the trend in emerging wireless access technologies, HSDPA supports end-user multi-class sessions comprising parallel flows with diverse Quality of Service (QoS) requirements, such as real-time (RT) voice or video streaming concurrent with non real-time (NRT) data service being transmitted to the same user, with differentiated queuing at the radio link interface. Hence, in this paper we present and evaluate novel radio link buffer management schemes for QoS control of multimedia traffic comprising concurrent RT and NRT flows in the same HSDPA end-user session. The new buffer management schemes—Enhanced Time Space Priority (E-TSP) and Dynamic Time Space Priority (D-TSP)—are designed to improve radio link and network resource utilization as well as optimize end-to-end QoS performance of both RT and NRT flows in the end-user session. Both schemes are based on a Time-Space Priority (TSP) queuing system, which provides joint delay and loss differentiation between the flows by queuing (partially) loss tolerant RT flow packets for higher transmission priority but with restricted access to the buffer space, whilst allowing unlimited access to the buffer space for delay-tolerant NRT flow but with queuing for lower transmission priority. Experiments by means of extensive system-level HSDPA simulations demonstrates that with the proposed TSP-based radio link buffer management schemes, significant end-to-end QoS performance gains accrue to end-user traffic with simultaneous RT and NRT flows, in addition to improved resource utilization in the radio access network.

Elastic-net prefiltering for two-class classification

A two-stage linear-in-the-parameter model construction algorithm is proposed aimed at noisy two-class classification problems. The purpose of the first stage is to produce a prefiltered signal that is used as the desired output for the second stage which constructs a sparse linear-in-the-parameter classifier. The prefiltering stage is a two-level process aimed at maximizing a model's generalization capability, in which a new elastic-net model identification algorithm using singular value decomposition is employed at the lower level, and then, two regularization parameters are optimized using a particle-swarm-optimization algorithm at the upper level by minimizing the leave-one-out (LOO) misclassification rate. It is shown that the LOO misclassification rate based on the resultant prefiltered signal can be analytically computed without splitting the data set, and the associated computational cost is minimal due to orthogonality. The second stage of sparse classifier construction is based on orthogonal forward regression with the D-optimality algorithm. Extensive simulations of this approach for noisy data sets illustrate the competitiveness of this approach to classification of noisy data problems.

Email categorization using multi-stage classification technique

This paper presents an innovative email categorization using a serialized multi-stage classification ensembles technique. Many approaches are used in practice for email categorization to control the menace of spam emails in different ways. Content-based email categorization employs filtering techniques using classification algorithms to learn to predict spam e-mails given a corpus of training e-mails. This process achieves a substantial performance with some amount of FP tradeoffs. It has been studied and investigated with different classification algorithms and found that the outputs of the classifiers vary from one classifier to another with same email corpora. In this paper we have proposed a multi-stage classification technique using different popular learning algorithms with an analyser which reduces the FP (false positive) problems substantially and increases classification accuracy compared to similar existing techniques.

Multi-classifier classification of spam email on an ubiquitous multi-core architecture

This paper presents an innovative fusion based multi-classifier email classification on a ubiquitous multi-core architecture. Many approaches use text-based single classifiers or multiple weakly trained classifiers to identify spam messages from a large email corpus. We build upon our previous work on multi-core by apply our ubiquitous multi-core framework to run our fusion based multi-classifier architecture. By running each classifier process in parallel within their dedicated core, we greatly improve the performance of our proposed multi-classifier based filtering system. Our proposed architecture also provides a safeguard of user mailbox from different malicious attacks. Our experimental results show that we achieved an average of 30% speedup at the average cost of 1.4 ms. We also reduced the instance of false positive, which is one of the key challenges in spam filtering system, and increases email classification accuracy substantially compared with single classification techniques.

Clustering based multi-label classification for image annotation and retrieval

This paper presents a novel multi-label classification framework for domains with large numbers of labels. Automatic image annotation is such a domain, as the available semantic concepts are typically hundreds. The proposed framework comprises an initial clustering phase that breaks the original training set into several disjoint clusters of data. It then trains a multi-label classifier from the data of each cluster. Given a new test instance, the framework first finds the nearest cluster and then applies the corresponding model. Empirical results using two clustering algorithms, four multi-label classification algorithms and three image annotation data sets suggest that the proposed approach can improve the performance and reduce the training time of standard multi-label classification algorithms, particularly in the case of large number of labels.

Dual-random ensemble method for multi-label classification of biological data

This paper presents a dual-random ensemble multi-label classification method for classification of multi-label data. The method is formed by integrating and extending the concepts of feature subspace method and random k-label set ensemble multi-label classification method. Experiemental results show that the developed method outperforms the exisiting multi-lable classification methods on three different multi-lable datasets including the biological yeast and genbase datasets.

Image to text translation by multi-label classification

This paper presents an image to text translation platform consisting of image segmentation, region features extraction, region blobs clustering, and translation components. A multi-label learning method is suggested for realizing the translation component. Empirical studies show that the predictive performance of the translation component is better than its counterparts when employed a dual-random ensemble multi-label classification algorithm.

Image to text translation by multi-label classification

This thesis includes the development of an architectural framework for the proposed image to text translation system containing four components. Selection of appropriate algorithms for the first three components developed three effective multi-label classification algorithms for the fourth component, i.e. the translation component, for different problem settings.

Efficient dimensionality reduction and one-class classification for content-based image retrieval

The thesis investigates various machine learning approaches to reducing data dimensionality, and studies the impact of asymmetric data on learning in image retrieval. Efficient algorithms are proposed to reduce the data dimensionality. Integration strategies for one-class classification are designed to address asymmetric data issue and improve retrieval effectiveness.

Empirical study of multi-label classification methods for image annotation and retrieval

This paper presents an empirical study of multi-label classification methods, and gives suggestions for multi-label classification that are effective for automatic image annotation applications. The study shows that triple random ensemble multi-label classification algorithm (TREMLC) outperforms among its counterparts, especially on scene image dataset. Multi-label k-nearest neighbor (ML-kNN) and binary relevance (BR) learning algorithms perform well on Corel image dataset. Based on the overall evaluation results, examples are given to show label prediction performance for the algorithms using selected image examples. This provides an indication of the suitability of different multi-label classification methods for automatic image annotation under different problem settings.