Padronização das plaquetas preparadas in house para atividade do cofator da ristocetina

Pós-graduação em Pesquisa e Desenvolvimento (Biotecnologia Médica) - FMB

Evaluation of the diagnostic value of the ELISA tests developed by using EgHF, Em2 and EmII/3-10 antigens in the serological diagnosis of alveolar echinococcosis

Alveolar echinococcosis (AE), caused by larva stage of Echinococcus multilocularis, is one of the lethal parasitic diseases of man and a major public health problem in many countries in the northern hemisphere. When the living conditions and habits in Turkey were considered in terms of relation with the life cycle of the parasite, it was suggested that AE has been much more common than reported mainly from the Eastern Anatolia region of Turkey. Since in vitro serologic diagnosis tests with high specificity for AE have not been used in our country, most of the cases with liver lesions were misdiagnosed by radiological investigations as malignancies. The aim of this study was to evaluate the diagnostic value of the in-house ELISA methods developed by using three different antigens (EgHF, Em2, EmII/3-10) in the serological diagnosis of AE. The study samples included a total of 100 sera provided by Bern University Parasitology Institute where samples were obtained from patients with helminthiasis and all were confirmed by clinical, parasitological and/or histopathological means. Ten samples from each of the cases infected by E.multilocularis, E.granulosus, Taenia solium, Wuchereria bancrofti, Strongyloides stercolaris, Ascaris lumbricoides, Toxocara canis, Trichinella spiralis, Fasciola hepatica and Schistosoma haematobium were studied. In the study, EgHF (E.granulosus hydatid fluid) antigens were prepared in our laboratory from the liver cyst fluids of sheeps with cystic echinococcosis, however Em2 (E.multilocularis metacestode-purified laminated layer) and EmII/3-10 (E.multilocularis recombinant protoscolex tegument) antigens were provided by Bern University Parasitology Institute. Flat bottom ELISA plates were covered with EgHF, Em2 and EmII/3-10 antigens in the concentrations of 2.5 µg, 1 µg and 0.18 µg per well, respectively, and all sera were tested by EgHF-ELISA, Em2-ELISA and EmII/3-10-ELISA methods. For each tests, the samples which were reactive above the cut-off value (mean OD of negative controls+2 SD) were accepted as positive. The sensitivity of the ELISA tests performed with EgHF, Em2 and Em2II/3-10 antigens were estimated as 100%, 90% and 90%, respectively, whereas the specificity were 63%, 91% and 91%, respectively. When Em2-ELISA and EmII/3-10-ELISA tests were evaluated together, the specificity increased to 96%. Our data indicated that the highest sensitivity (100% with EgHF-ELISA) and specificity (96% with Em2-ELISA + EmII/3-10-ELISA) for the serodiagnosis of AE can be achieved by the combined use of the ELISA tests with three different antigens. It was concluded that the early and accurate diagnosis of AE in our country which is endemic for that disease, could be supported by the use of highly specific serological tests such as Em2-ELISA ve EmII/3-10-ELISA contributing radiological data.

Analysis of house prices to assess economic impacts of new public transport infrastructure: Madrid metro line 12.

Transportation infrastructure is known to affect the value of real estate property by virtue of changes in accessibility. The impact of transportation facilities is highly localized as well, and it is possible that spillover effects result from the capitalization of accessibility. The objective of this study was to review the theoretical background related to spatial hedonic models and the opportunities that they provided to evaluate the effect of new transportation infrastructure. An empirical case study is presented: the Madrid Metro Line 12, known as Metrosur, in the region of Madrid, Spain. The effect of proximity to metro stations on housing prices was evaluated. The analysis took into account a host of variables, including structure, location, and neighborhood and made use of three modeling approaches: linear regression estimation with ordinary least squares, spatial error, and spatial lag. The results indicated that better accessibility to Metrosur stations had a positive impact on real estate values and that the effect was marked in cases in which a house was for sale. The results also showed the presence of submarkets, which were well defined by geographic boundaries, and transport fares, which implied that the economic benefits differed across municipalities.

The Economic Value of Residential Land in Historical Areas: An Application of the Residual Method to the Secondary Market

Land value bears significant weight in house prices in historical town centers. An essential aim for regulating the mortgage market, particularly in the financial and property crisis that countries such as Spain are undergoing, is to have at hand objective procedures for its valuation, whatever the conditions (location, construction, planning). Of all the factors contributing to house price make-up, the land is the only one whose value does not depend on acquisition cost, but rather on the location-time binomial. That is to say, the specific circumstances at that point and at the exact moment of valuation. For this reason, the most commonly applied procedure for land valuation in town centers is the use of the residual method: once the selling price of new housing in a district is known, the other necessary costs and expenses of development are deducted, including those of building and the developer’s profit. The value left is that of the land. To apply these procedures it is vital to have figures such as building costs, technical fees, tax costs, etc. But, above all, it is essential to obtain the selling price of the new housing. This is not always feasible, on account of the lack of newbuild development in this location. This shortage of information occurs in historical town cities, where urban renewal is slight due to the heritage-protection policies, and where, nevertheless there is substantial activity in the secondary market. In these circumstances, as an alternative for land valuation in consolidated urban areas, we have the adaptation of the residual method to the particular characteristics of the secondary market. To these ends, there is the proposal for the appreciation of the dwelling which follows, in a backwards direction, the application of traditional depreciation methods proposed by the various valuation manuals and guidelines. The reliability of the results obtained is analyzed by contrasting it with published figures for newly-built properties, according to different rules applied in administrative appraisals in Spain and the incidence of an eventual correction due to conservation state.

Donde se rompen las nubes : Lever House : Nueva York 1950

En la victoriosa América de la posguerra, origen de la nueva configuración del mundo occidental, surgió una singular obra que iba a representar la abstracción de los valores universales de la arquitectura moderna. La construcción de la Lever House en el Nueva York de 1950 no sólo iba a propiciar un punto de inflexión en la Historia del rascacielos y de la arquitectura corporativa americana, definiría a su vez un nuevo paradigma a partir del cual se establecerían generalizados modelos para la construcción del futuro. La oportuna confluencia entre los acentos pragmáticos del mundo capitalista americano y las formulaciones positivistas de la arquitectura moderna europea, convenientemente recogidas como manifiesto construído del Estilo Internacional, supusieron resolver de manera definitiva el prolongado dilema americano de cómo debería ser la adecuada representación del edificio corporativo moderno. El estudio se detendrá en el valor de la metáfora, la conversación y la oportunidad de una obra cuya proyección trascendió ampliamente las aspiraciones de sus creadores. Provocando una fuerte idealización que llegaría hasta nuestros días. ABSTRACT In the postwar victorious America, origin of the new configuration of the Western World, a unique work will represent the abstraction of the universal values of modern architecture. The construction of the Lever House in the New York of 1950 would not only bring about a turning point in the history of American skyscrapers and corporate architecture, in turn define a new paradigm from which generalized models for building the future. The timely confluence between pragmatic accents of the American capitalist world and the positivistic formulations of the modern European architecture, conveniently collected as constructed manifesto of the International Style, definitively accounted to resolve that old American dilemma of how should be the adequate representation of the Modern corporate building. The study will focus on the value of metaphor, conversation and the opportunity of a work whose projection widely transcended the aspirations of its creators. Causing an strong idealization would reach to nowadays.

Battlefield of Spotsylvania Court House, Virginia and vicinity, 1864 (Raster Image)

This layer is a georeferenced raster image of the historic paper map: Map of the battle field of Spottsylvania C.H. : showing the field of operations of the Army of the Potomac commanded by Maj. Gen. George G. Meade U.S.A., from May 8th to 21st, 1865 [i.e. 1864], surveyed under the orders of Bvt. Col. J.C. Duane, Major of Engineers, Chief Engineer, Army of the Potomac, by Bvt. Maj. C.W. Howell, 1st Lieut. of Engineers ; assisted by Messrs. L.C. Oswell, L. Bell, and R.B. Talfor ; J. Bien, lithographer, New York. It was published ca. 1865. Scale [1:15,840]. Covers area surrounding Spotsylvania and Spotsylvania Battlefield, Virginia. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Virginia State Plane North Coordinate System (in Meters) (Fipszone 4501). All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, or other information associated with the principal map. This map shows features such as roads, drainage, dwellings with names of inhabitants, vegetation, Union and Confederate troop lines and defenses, and more. Relief shown by hachures. Includes note. This layer is part of a selection of digitally scanned and georeferenced historic maps of the Civil War from the Harvard Map Collection. Many items from this selection are from a collection of maps deposited by the Military Order of the Loyal Legion of the United States Commandery of the State of Massachusetts (MOLLUS) in the Harvard Map Collection in 1938. These maps typically portray both natural and manmade features, in particular showing places of military importance. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.

Provision of facilities management services in Sri Lankan commercial organisations: is in-house involvement necessary?

Purpose - The purpose of this paper is to explore sourcing strategies for facilities management services using core-competency theory of outsourcing. The aim is to develop a screening framework for deciding the suitability of outsourcing versus in-house delivery for these services based on three levels of managerial functions prevalent in a typical commercial organization.

Design/methodology/approach - A questionnaire survey was administered for facilities managers in Sri Lanka to investigate the relative importance of these managerial functions for facilities management services and obtain their opinions on the best delivery mode. The managerial functions were derived from a literature review and verified using three semi-structured interviews prior to the questionnaire survey design.

Findings - The findings showed that facilities management services that are aligned to strategic functions are suitable for in-house delivery, while those that are aligned to tactical and operational functions for outsourcing.

Research limitations/implications - Further studies should be conducted and extended to other types of organizations beyond commercial ones. Secondly, the quantitative study employed a smaller sample (n = 40), and the survey items were based on the review of literature which was verified using a very small number of interviews (n = 3).

Practical implications - The proposed framework can be utilized when choosing the best facilities management approach for commercial organizations in developing countries such as Sri Lanka.

Originality/value - This study contributes to the body of knowledge on the subject of facilities management by exploring the context in Sri Lankan which has not previously been done.

The relationship between house prices and demographic variables: an Australian case study

Purpose – The process for examining the value of house prices in an urban city has given limited attention, if any, to demographic variables associated with urban geography. Although the disciplines of property/real estate and demography have moved closer, little progress has been made when modelling house prices using population-related data in the field of urban geography to explain the level of house prices.

Design/methodology/approach – This paper proposes an innovative model to examine the influence of population variables on the level of house prices. It used a two-stage approach as follows: principal components analysis (PCA) identified social dimensions from a range of demographic variables, which were then retained for further analysis. This information was sourced from two Australian Bureau of Statistics censuses undertaken involving all Melbourne residents during 1996, 2001, 2006 and 2011; multiple regression analysis examined the relationship between the retained factor scores from the PCA (as independent variables) and established residential house prices (as the dependent variable).

Findings – The findings confirm the demographic profile of each household, which is directly related to their decisions about housing location and house prices. Based on a case study of Melbourne, Victoria, it was demonstrated that households with specific demographic characteristics are closely related to a certain level of house prices at the suburban level.

Originality/value – This is an innovative study which has not been previously undertaken for an extended period of time to facilitate an analysis of change over time.