Influence of stocking density on the culture potential of freshwater catfish Pangasius pangasius in pond

Influence of stocking density on the production of freshwater catfish Pangasius pangasius with formulated feed in ponds was studied. The fish fry were collected from the Meghna river near Chandpur which is a natural breeding ground of this fish. Three stocking densities chosen during this experiment were 5000, 8000 and 11000 fry/ha with an initial weight of 42.57 ±2.51 g. The formulated feed was prepared in the laboratory which contained 45% fish meal, 30% mustard oil cake, 15% wheat bran and 10% rice bran to supply 40.48% protein in feed. The growth of P. pangasius in terms of net weight gain was 409.49, 266.49 and 236.37 g at the 5000/ha, 8000/ha and 11000/ha stocking densities respectively, which was statistically significant (p<0.05). High food conversion ratio (FCR) was also observed during this research study which ranged between 7.06 to 7.72. A production of 2.6 tons/ha at the stocking density of 11000/ha; 2.13 tons/ha at the stocking density of 8000/ha and 2.04 tons/ha at the stocking density of 5000/ha was obtained, while the growth of individual fish at the end of experiment was in opposite order. The result of this experiment indicates that stocking density has significant influence on the culture potential of freshwater catfish P.pangasius.

Over-wintering performance of mixed sex and monosex tilapia (Oreochromis niloticus L.) in Bangladesh

An experiment was conducted for six months in 6 experimental ponds (each size 80 of m2) to assess the over-wintering performance between mixed sex and monosex tilapia, Oreochromis niloticus. The experiment was carried out with two treatments each with three replicates. In the first treatment (T1), mixed sex tilapia were stocked in 3 ponds with a mean initial of 4.80±0.18 g. In the second treatment (T2), monosex tilapia were stocked in another 3 ponds with a mean initial weight of 4.81 ±0.20 g. Each pond was stocked with 250 fingerlings. Fish were fed at the rate of 6% of fish body weight at the beginning. The feeding rate was gradually reduced to 2% for the third month and finally increased to 3% for rest of the period. Water quality was monitored fortnightly and the ranges were: temperature17.86-29.10°C, dissolved oxygen 4.25-6.10 mg/1, pH 6.97-7.20 and transparency 24.10-36.50 cm. After 6 months of rearing monosex tilapia attained a significantly (pfood conversion ratio and survival (%)values between the mixed sex and monosex tilapia. The production of monosex tilapia (3723.10 kg/ha) was about 32% higher than that of mixed sex tilapia (2776.28 kg/ha). The net profit/ha generated from the 6 months culture period was calculated as Tk. 43,311.45 and. 69,277.32/- for mixed sex and monosex tilapia respectively. The results of the present study suggested that it is possible to successfully culture tilapia during the winter period and the culture of monosex tilapia is more profitable due to its higher growth rate than that of mixesex tilapia.

Effect of feeding frequency on the growth of common carp (Cyprinus carpio L.) fry

A laboratory-feeding trail was conducted for 45 days with fry of common carp Cyprinus carpio L. (0.45±0.03g) in aquaria in a static indoor fish rearing system. The fry were fed on a pelleted diet containing 33% crude protein having fishmeal as major protein source. The fish fry in five treatments A, B, C, D, and E, each with two replicates were fed on 5% daily ration divided into different feeding frequencies of 2, 3, 4, 5 and 6 times a day respectively in order to observe the growth performance. Each replicate contained 15 fry having total initial weight of 6.87±0.31g. At the end of the feeding trial, significantly different and higher (p<0.05) growth response was observed in treatment C having a feeding frequencies of 4 times a day. Significantly the highest and the lowest percent growth of 334.30 and 218.91% were observed in fish fed on the diet (Treatment C) with 4 times and (Treatment A) 2 times feeding frequencies per day, respectively. Food conversion ratio (FCR) of 1.78 was significantly higher (p

Effect of stocking density on the production of common carp (Cyprinus carpio Lin.) in cages at Kaptai lake, Bangladesh

Growth, survival and food conversion ratio (FCR) of Cyprinus carpio in cages at different stocking density were studied in Kaptai lake. Fingerlings of common carp (12.47 ± 4.29 g) were stocked at 25/m2 , 50/m2 and 75/m2 each with replication in six floating net cages each of Sm x Sm x 4m and reared for 240 days. The growth rate was inversely related to the stocking density with the mean weights of 325.5 ± 11.74 g, 268.99± 8.44 g and 167.0 ± 11.66 g at low, medium and high densities, respectively. At harvest, standing crop biomass averaged 7 .82, 12.83 and 11.58 kg with the survival of 98.9%, 97.6% and 94.4% and food conversion ratios of 4.51, 3.82 and 4.21 for the above three densities, respectively. Weight gain and production at density 25 fish/m 2 were significantly different (p< 0.01) from other two densities. Water quality was not affected in the cages having different stocking densities.

Growth performance and nutrient utilization of catfish Clarias gariepinus fed varying inclusion level of fermented unsieved yellow maize

This study was conducted using 150 fish of Clarias gariepinus to investigate the growth performance and nutrient utilization of Clarias gariepinus fed five treatment diets containing varying inclusion level of fermented unsieved maize. The diets were grouped into CT, T1, T2, T3, and T4 with inclusion levels of 25%, 50%, 75%, and 100% of fermented unsieved maize respectively. Highest weight gain was recorded in T4 with value of 10.24 and lowest weight was recorded in CT with 9.17. High FCR were observed in T2 with value of 0.70 and lower value was observed in T4 with value of 0.62. While, T2, T3, and T4 have highest survival rates with values of 90% in each treatment CT and T1 recorded 80% and 70% respectively. There was a significant (p< 0.05) difference between the food conversion ratios treatment T4 with the best value and other treatments. There was a significant (p< 0.05) difference between the levels of fermented unsieved maize inclusion and the specific growth rate of the experimental fish. The highest value of protein level and feed efficiency were observed in T4 at significant difference level (p< 0.05) than other treatments. It was concluded that fermentation of maize in fish feed has positive effects on the nutritional value of the feed. It is recommend that fermented maize can replace raw maize in fish feed diet for growth performance. KEYWORDS: Fermentation, yellow maize, Clarias gariepinus, Fish, Feed.

Growth performance and nutrient utilization of catfish Clarias gariepinus fed varying inclusion level of fermented unsieved yellow maize

This study was conducted using 150 fish of Clarias gariepinus to investigate the growth performance and nutrient utilization of Clarias gariepinus fed five treatment diets containing varying inclusion level of fermented unsieved maize. The diets were grouped into CT, T1, T2, T3, and T4 with inclusion levels of 25%, 50%, 75%, and 100% of fermented unsieved maize respectively. Highest weight gain was recorded in T4 with value of 10.24 and lowest weight was recorded in CT with 9.17. High FCR were observed in T2 with value of 0.70 and lower value was observed in T4 with value of 0.62. While, T2, T3, and T4 have highest survival rates with values of 90% in each treatment CT and T1 recorded 80% and 70% respectively. There was a significant (p< 0.05) difference between the food conversion ratios treatment T4 with the best value and other treatments. There was a significant (p< 0.05) difference between the levels of fermented unsieved maize inclusion and the specific growth rate of the experimental fish. The highest value of protein level and feed efficiency were observed in T4 at significant difference level (p< 0.05) than other treatments. It was concluded that fermentation of maize in fish feed has positive effects on the nutritional value of the feed. It is recommend that fermented maize can replace raw maize in fish feed diet for growth performance.

Effects of dietary supplementation of commercial human probiotic and antibiotic on the growth rate and content of intestinal microflora in ornamental fishes

The effects of dietary supplementation of commercial human probiotic, Lactobacil and antibiotic, oxytetracycline on the growth, survival, disease resistance and content of intestinal microflora in two ornamental fishes, viz., goldfish, Carassius auratus and swordtail, Xiphophorus helleri were studied. The total wet weight gain, food conversion ratio and specific growth rate of C. auratus did not vary significantly (p>0.05) among treatments. While in X. helleri, significant differences existed in the total wet weight gain, survival, food conversion ratio and specific growth rate among treatment groups (p<0.05). The counts of antibiotic resistant bacteria in fish gut increased with days of culture in all the treatments and the increase was more in antibiotic fed fishes. A reduction in the development of antibiotic resistance among the bacterial flora of fish gut was noticed in probiotic fed groups of C auratus and X. helleri. The results of the present study revealed that the effects of human probiotic on the growth, survival and disease resistance of ornamental fishes are variable and difficult to reproduce the similar effect on different species.

Suitable stocking density of tilapia in an aquaponic system

An aquaponic system was studied through the integrated culture of mono-sex GIFT and two types of vegetables viz. morning glory, Ipomoea reptans and taro, Colocasia esculenta in a recirculating system for 15 weeks. Tilapia fry of uniform size of 0.76 g were released in three treatments (stocking densities): 106 fish/m³ (T1), 142 fish/m³ (T2) and 177 fish/m³ (T3) to assess the effect of stocking density on the growth performance of fish. Fish were fed with a commercial feed containing 25% protein. Weight gain (g) of tilapia ranged from 19.41 to 32.67 g and was inversely related with stocking density. Percent weight gain varied between 2553.99 and 4298.68% and was significantly different among the treatments. SGR ranged from 3.09 to 3.59% per day and varied significantly. FCR varied from 2.19 to 2.69 and had a positive correlation with stocking density. The highest survival rate (%) was achieved in T1 (99%) followed by T2 (98%) and T3 (96%). Production of fish ranged from 3.43 to 3.52 kg/m³ and was inversely related with stocking density. The present study demonstrated that 106 fish/m³ was the best stocking density in terms of growth, food conversion ratio, survival and production for tilapia culture in the aquaponic system.

Effects of stocking density on growth and production of GIFT (Oreochromis niloticus)

The study was carried out to assess the effects of stocking density on growth and production of GIFT for a period of 100 days. Three stocking densities were used 150, 200 and 250 fish/decimal; designated as treatment T1, T2 and T3 respectively having two replicates for each. Commercial pellet feeds were fed at the rate of 30% body weight up to first 10 days and then gradually it was readjusted to 22%, 18%, 15%, 12%, 10%, 8%, 6%, 5% and 4% respectively after every 10 days interval. The result showed that the fish in the treatment T1 stocked with the lowest stocking density (150 fish/dec) resulted in best individual weight gain (148.65g) followed by those in treatment T2 and T3 respectively. The specific growth rates (SGR) at every 10 days were ranged from 6.59 to 1.11 in different treatments during the experimental period. The food conversion ratio (FCR) values ranged between 1.82 to 2.03 with treatment T1 showing the lowest FCR. The survival rate ranged between 84 to 92%. Treatment T1 and treatment T2 showed significantly higher survival than Treatment T3. The fish production rate in treatment T1, T2 and T3 were 18.58, 23.87 and 26.78 kg/decimal respectively.

Interactions of dietary levels of protein and energy on rainbow trout (Oncorhynchus mykiss) in desert brackish water

A 3x3 factorial experiment was conducted to determine the optimum protein to energy (P/E) ratio for rainbow trout in brackish water. Three crud protein levels and three energy levels at each protein level were utilized. Diets were made in semi-purified that in all of them fish meal, casein and gelatin as the sources of protein and dextrin, starch and oil as the sources of energy were used. Each of experimental diets was fed to triplicate groups of 20 fish with an average individual weight of 81.5 g in 9 2000-1 flow trough fiberglass tanks. During this experiment water temperature, dissolved oxygen, PH and EC were 15±2°C, 6.5-8.1 mg/1, 7.7-8.6 and 25400 grills respectively. The diets were fed at a rate between 1.6-2 wet body weight% per day depended to water temperature in three equal rations and adjusted two weekly for 84 days. At each of protein levels, weight gain percent (%WG), average daily growth percent (%ADG), protein efficiency ratio (PER), apparent net protein utilization percent (%ANPU), or percent of protein deposited, specific growth rate (SGR) and condition factor (CF) were found to increase and food conversion ratio (FCR) was found to decrease with an increasing energy levels from 370 to 430 Kcal/100g. Fish fed a 35% protein, 430 Kcal/100g energy diet with a P/E ratio of 81.4 mg protein/ Kcal PFV energy, attained the best growth performance. Fat and moisture of carcass were affected by protein and energy levels of test diets while protein and ash of carcass were relatively constant in different treatments.

Physiological advantages may contribute to successful invasion of the exotic Cyprinus carpio into the Xingyun Lake, China

Feeding and growth traits of Cyprinus carpio and Cyprinus pellegrini (both at age-0) were compared in three experiment, in an attempt to analyze potential causes for the displacement of the native C. pellegrini in the Xingyun Lake, Yuxi, Yunan, China. Experiment I was conducted in water which fluctuated between 15 and 20 degrees C. Experiment II and III were conducted in a laboratory and water temperature was maintained between 20 degrees C and 25 degrees C, respectively. Three common trends were noted for all three experiments: (1) feeding rate of C. carpio was lower than that of C. pellegrini, and this difference was found to be significant in experiment I; (2) growth rate of C. carpio was higher than C. pellegrini, and the difference was found to be significant in experiment II; (3) food conversion efficiency and energy retention efficiency for C. carpio were higher than those of C. pellegrini, and significant differences were noted in experiment I and II. Since the growth period for fish in the Xingyun Lake generally occurs when water temperatures are between 15 and 25 degrees C, it can be suggested that C. carpio has advantages over C. pellegrini in growth and food utilization efficiency, and lower food consumption than C. pellegrini. These physiological traits of C. carpio might allow this species to be more resistant to food shortage and predation, and may be partially responsible for the displacement of C. pellegrini by C. carpio.

Fast-growing transgenic common carp mounfing compensatory growth

Compensatory growth is a phase of accelerated growth apparent when favourable conditions are restored after a period of growth depression. To investigate if F-2 common 'all-fish' growth hormone gene transgenic common carp (Cyprinus carpio) could mount compensatory growth, a 9 week study at 29 degrees C was performed. The control group was fed to satiation twice a day throughout the experiment. The other two groups were deprived of feed for 1 or 2 weeks, respectively, and then fed to satiation during the re-feeding period. At the end of the experiment, the live masses of fish in the deprived groups were still significantly lower than those of the controls. During the re-feeding period, size-adjusted mean specific growth rates and mean feed intakes were significantly higher in the deprived fish than in the controls, indicating a partial compensatory growth response in these fish. No significant differences were found in food conversion efficiency between the deprived and control fish during re-feeding, suggesting that hyperphagia was the mechanism responsible for increased growth rates. The proximate composition of the deprived fish at the end of the experiment was similar to that of the control fish. This study is, to our knowledge, the first to report that fast-growing transgenic fish can achieve partial compensation of growth following starvation. (c) 2007 The Authors Journal compilation (c) 2007 The Fisheries Society of the British Isles.

Dietary methionine requirement for juvenile rockfish, Sebastes schlegeli

A 65-day growth trial was conducted at 19.5 degrees C to determine the optimal dietary methionine for juvenile rockfish, Sebastes schlegeli. Semipurified diets were formulated to be isonitrogenous and isoenergetic. Fish meal was used as intact protein source and crystal amino acid was used as a part of dietary protein. Six experimental diets were formulated to contain 5.8, 10.8, 15.8, 20.8, 25.8 and 30.8 g kg(-1) dietary methionine. The results showed that dietary methionine significantly affected specific growth rate, weight gain, food conversion ratio, protein productive value (PPV), energy retention efficiency, carcass index and body composition. No significant difference was found in hepatosomatic index. The dietary methionine requirement for maximum growth was 13.7 g kg(-1) dry matter or 28.0 g kg(-1) of dietary protein when cystine content was 1.2 g kg(-1) dry matter.

Growth hormone gene transfer in common carp

The first successful case of transgenic fish was achieved in 1984. It is in a model system that the integration and expression of recombinant human growth hormone (hGH) in host red common carp (Cyprinus carpio, red var.) have been thoroughly studied. Recently, the integration sites have been recovered and characterized. Compared with non-transgenic peers, hGH-transgenic fish are prior in dietary utilization and growth performance. In view of bio-safety and bio-ethics, an "all-fish" construct CAgcGH, grass carp growth hormone fused with common carp P-actin promoter, has been generated and transferred into Yellow River carp (C carpio, local strain in Yellow River) fertilized eggs. Under middle-scale trial, CAgcGH-transgenics show higher growth rate and food conversion efficiency than the controls, which is consistent to laboratory findings. To avoid the potential impact of transgenic fish on the environment, a sterile strain of transgenic triploid fish has been successfully produced. The "all-fish" transgenic common carp is also approved safe enough as daily food, according to a test based on the pathological principles of new medicines issued by the Ministry of Health of China. The "all-fish" transgenic common carp with growth enhancement is now ready for market, but looking for governmental authorization. (C) 2003 Editions scientifiques et medicales Elsevier SAS and Ifremer/IRD/Inra/Cemagref. All rights reserved.

The influence of diets containing dried bivalve feces and/or powdered algae on growth and energy distribution in sea cucumber Apostichopus japonicus (Selenka) (Echinodermata : Holothuroidea)

In recent years, bivalve feces and powdered algae have been used as the food sources of holothurians in China. In this study, growth and energy budget for sea cucumber Apostichopus japonicus (Selenka) with initial wet body,veights of 32.5 1.0 g (mean +/- SE, n=45) when fed with five different granule diets containing dried bivalve feces and/or powdered algae in water temperature 13.2-19.8 degrees C and salinity 30-32ppt were quantified in order to investigate how diets influence growth and energy distribution and to find out the proper diet for land-based intensive culture of this species. Results showed that diets affected the food ingestion, feces production, food conversion efficiency and apparent digestive ratios, hence the growth and energy budget. Sea cucumbers fed with dried feces of bivalve showed poorer energy absorption, assimilation and growth than individuals fed with other four diets; this could be because feces-drying process removed much of the benefits. Dried bivalve feces alone, therefore, were not a suitable diet for sea cucumbers in intensive cultivation. The mixed diets of feces and powered algae showed promising results for cultivation of sub-adult Apostichopus japonicus, while animals fed with powdered algae alone, could not obtain the best growth. According to SGR of tested animals, a formula of 75% feces and 25% powdered algae is the best diet for culture of this species. Extruded diets were used in the present experiment to overcome shortcomings of the traditional powdered feeds, however, it seems a conflict exists between drying bivalve feces to form extruded diets and feeding sea cucumbers with fresh feces which contain beneficial bacteria. Compared with other echinoderms, in holothurians the energy deposited in growth is lower and the energy loss in feces accounts for the majority of the ingested energy. Such detailed information could be helpful in further development of more appropriate diets for culture of holothurians. (c) 2006 Elsevier B.V. All rights reserved.